Activities in Family	amylomaltase or 4-α-glucanotransferase (EC 2.4.1.25)
Mechanism	Retaining
Clan	GH-H
3D Structure Status	( β / α ) 8
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Glu
External resources	CAZypedia;
Statistics	GenBank accession (5994); Uniprot accession (794); PDB accession (22); 3D entries (8); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
amylomaltase (MalM;Aq_723)	2.4.1.25	Aquifex aeolicus VF5	AAC06897.1 CAE51853.1 NP_213497.1	O66937	1TZ7[A,B] 2.15
amylomaltase (CgAM;MalQ;cg2523)	2.4.1.25	Corynebacterium glutamicum ATCC 13032	BAB99690.1 CAF20639.1 CCH25437.1 NP_601497.1	Q8NNA7	5B68[A] 1.70 5JJH[A] 1.80
4-α-glucanotransferase (MalQ;b3416)	2.4.1.25	Escherichia coli str. K-12 substr. MG1655	AAC76441.1 AAA24106.1 AAA58214.1 AIZ89581.1 ALI38902.1 AMC96303.1 AUG18120.1 AVI53577.1 NP_417875.1	P15977	4S3P[A,B] 2.80 4S3Q[A,B,C] α-D-Glcp-(1-4)-α-D-Glcp 2.10 4S3R[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp 2.10
amylomaltase (MalQ)	2.4.1.25	Thermus aquaticus ATCC 33923	BAA33728.1	O87172	1CWY[A] 2.00 1ESW[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.90 5JIW[A] 1.73
4-α-glucanotransferase / amylomaltase (TBGT)	2.4.1.25	Thermus brockianus	AAY89060.1	Q2VJA0	2X1I[A] <carb_like_INS> 2.36
4-α-glucanotransferase / amylomaltase (TTHA1261)	2.4.1.25	Thermus thermophilus HB8	BAD71084.1 CAE51852.1	Q5SIV3	1FP8[A] 2.30 1FP9[A] 3.10 2OWC[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp-(1-4)-ASP 2.05 2OWW[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp-(1-4)-ASP α-D-4-deoxy-Glcp 2.20 2OWX[A] 2.50
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
4-α-glucanotransferase (Dpe1;AtDPE1;At5g64860/MXK3.9) (plastidial)	2.4.1.25	Arabidopsis thaliana	BAA97298.1 AAK59831.1 AAL87397.1 AED97962.1 NP_201291.1	Q9LV91	5CPQ[A,B] 2.13 5CPS[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.80 5CPT[A,B] α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-ASP 2.30 5CQ1[A,B] α-D-Glcp α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-ASP 2.30 5CSU[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp 2.53 5CSY[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-ASP α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp 2.05
4-α-glucanotransferase (DpeP; D-enzyme)	2.4.1.25	Solanum tuberosum	CAA48630.1	Q06801	1X1N[A] 1.80
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