Glycoside Hydrolase Family 74

Activities in Family	Oligoxyloglucan reducing-end-specific cellobiohydrolase (EC 3.2.1.150); Xyloglucan-specific endo-β-1,4-glucanase / endo-xyloglucanase (EC 3.2.1.151); Endo-β-1,4-glucanase (EC 3.2.1.4);
Mechanism	Inverting e
3D Structure Status	7-fold β-propeller
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Asp (experimental)
External resources	CAZypedia;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (1540); Uniprot accession (35); PDB accession (19); 3D entries (12); cryst (0)

Summary

Download GH74 (2011) Taxonomic display Structure (12) Characterized (37)

Bacteria

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

β-1,4-xyloglucan hydrolase / xyloglucanase (XghA) (Xgh74A)

3.2.1.151

Acetivibrio thermocellus F7/YS

CAE51306.1

Q70DK5

2CN2[A,B,C,D]		2.1
2CN3[A,B]	α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp β-D-Galp-(1-2)-α-D-Xylp-(1-6)[α-D-Xylp-(1-6)-β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp	1.95

avicelase (AviIII;AcGH74;Acel_0618) (Cel74A)

Acidothermus cellulolyticus 11B

ABK52391.1
ACE14916.1
ACE14917.1
ACE14920.1
ACS09146.1
ACS09147.1
ACS09150.1

4LGN[A]

1.82

endo-xyloglucanase (Calla_2311)

3.2.1.151

Caldicellulosiruptor acetigenus 6A

AEM74844.1

G2PXX7

6P2M[A]

β-D-Galp-(1-2)-α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp

1.98

endo-xyloglucanase (CjGH74; CJA_2477) (Gly74A)

3.2.1.151

Cellvibrio japonicus Ueda107

ACE84745.1

B3PKK9

5FKQ[A,B]		1.71
5FKR[A]		2.28
5FKS[A]	α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp β-D-Galp-(1-2)-α-D-Xylp-(1-6)[α-D-Xylp-(1-6)-β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp	1.99
5FKT[A,B]		1.52

endo-xyloglucanase (Niako_1751)

3.2.1.151

Niastella koreensis GR20-10

AEV98114.1

G8TAF2

6P2L[A]

α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp

1.08

endo-xyloglucanase (PGRAT_12815)

3.2.1.151

Paenibacillus graminis DSM 15220

AIQ68396.1

A0A089M3R3

6P2N[A]

1.35

endo-xyloglucanase (CD191_11325)

3.2.1.151

Paenibacillus odorifer CBA7130

AWV33160.1

6MGJ[A,B,C,D,E,F,G,H]		2.00
6MGK[A,B,C,D]	β-D-Galp-(1-2)-α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp β-D-Galp-(1-2)-α-D-Xylp-(1-6)[α-D-Xylp-(1-6)-β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp	2.10
6MGL[A]	α-D-Xylp-(1-6)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp	1.50

endo-xyloglucanase (M271_10845)

3.2.1.151

Streptomyces rapamycinicus NRRL 5491

AGP53770.1

A0A0A0NH71

6P2O[A,B]

α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
β-D-Galp-(1-2)-α-D-Xylp-(1-6)[α-D-Xylp-(1-6)-β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp

1.88

SACTE_0562

Streptomyces sp. SirexAA-E

AEN08502.1

5JWZ[A,B]

1.70

xyloglucanase (Xgh; Xcc-Xgh; XCC1752)

3.2.1.151

Xanthomonas campestris pv. campestris str. ATCC 33913

AAM41043.1
NP_637119.1

Q8P9U5

7KN8[A,B]