Glycoside Hydrolase Family 74

Known Activities	endoglucanase (EC 3.2.1.4); oligoxyloglucan reducing end-specific cellobiohydrolase (EC 3.2.1.150); xyloglucanase (EC 3.2.1.151)
Mechanism	Inverting
3D Structure Status	7-fold β-propeller
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Asp (experimental)
External resources	CAZypedia;
Commercial Enzyme Provider(s)	NZYTech; PROZOMIX;
Statistics	GenBank accession (133); Uniprot accession (66); PDB accession (6); 3D entries (4); cryst (0)

Summary

All (111) Bacteria (93) Eukaryota (18) Structure (4 ) Characterized (15)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
β-1,4-xyloglucan hydrolase / xyloglucanase (XghA) (Xgh74A)	3.2.1.151	Clostridium thermocellum F7/YS	CAE51306.1	Q70DK5	2CN2[A,B,C,D] 2CN3[A,B]
xyloglucanase (Xeg)	3.2.1.151	Jonesia DSM 14140	CAD58415.1
xyloglucanase (XEG74) (Xeg74)	3.2.1.151	Paenibacillus sp. KM21	BAE44527.1	Q3MUH7
xyloglucan-specific endo-β-1,4-glucanase (SCO6545)	3.2.1.151	Streptomyces coelicolor A3(2)	CAA20642.1 NP_630626.1	O86727
xyloglucanase (Tfu_1612) (Xeg74)	3.2.1.151	Thermobifida fusca YX	AAZ55647.1 ZP_00056977.1	Q47PH2	1WWO[1]
endo-β-1,4-glucanase (TM0305) (Cel74)	3.2.1.4	Thermotoga maritima MSB8	AAD35393.1 NP_228117.1	Q9WYE1
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
avicelase III (Cmc2;AviIII;FIII Avicelase)	3.2.1.4	Aspergillus aculeatus F-50	ACE14921.1 ACS09151.1 BAA29031.1	O74170
oligoxyloglucan reducing end-specific xyloglucanobiohydrolase (OREX;AN1542.2)	3.2.1.150	Aspergillus nidulans FGSC A4	EAA64249.1
xyloglucanase C (EglC)	3.2.1.151	Aspergillus niger CBS 120.49 / N400	AAK77227.1	Q8TFP1
xyloglucanase (Xgl) (peptide fragment) (Xgl74A)	3.2.1.151	Chrysosporium lucknowense
oligoxyloglucan reducing end-specific cellobiohydrolase (OXG-RCBH)	3.2.1.150	Geotrichum sp. M128	BAC22065.1 CAF02212.1 CAF02249.1	Q8J0D2	1SQJ[A,B] 2EBS[A,B]
xyloglucanase (Xeg)	3.2.1.151	Geotrichum sp. M128	BAD11543.1	Q764N8	3A0F[A]
xyloglucanase (Egl6) (Cel74A)	3.2.1.151	Hypocrea jecorina QM6A	AAP57752.1 ABH71452.1 ABJ18610.1	Q7Z9M8
xyloglucanase (Xgh74B)	3.2.1.151	Phanerochaete chrysosporium K-3	BAF95189.1	A9CSH7
β-glucosidase (P21) (peptide fragment)	3.2.1.151	Stachybotrys sp. N1

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.