Glycoside Hydrolase Family 68 / Subf 2

Activities in Sub Family[retaining] levansucrase / sucrose:fructan 6-fructosyltransferase (6-SFT) (EC 2.4.1.10);
[retaining] inulosucrase / sucrose:fructan 1-fructosyltransferase (EC 2.4.1.9);
β-fructofuranosidase / invertase (EC 3.2.1.26);
Mechanism Retaining a
ClanGH-J
3D Structure Status5-fold β-propeller
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorGlu
External resourcesCAZypedia;
Statistics GenBank accession (1273); Uniprot accession (36); PDB accession (22); 3D entries (7); cryst (0)
Archaea
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 inulosucrase (InuHj; HacjB3_04715) 2.4.1.9 Halalkalicoccus jeotgali B3 ADJ14334.1 D8J9C2
7BJ4[A,B,C,D,E,F,G,H,I,J] α-D-Glcp-(1-1)-<non_carb>
α-D-Glcp-(1-2)-β-D-Fruf
2.72
7BJ5[A,B,C,D,E,F,G,H,I,J] 2.75
7BJC[A,B,C,D,E,F,G,H,I,J] α-D-Glcp-(1-2)-β-D-Fruf
3.11
2
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 β-fructofuranosidase (Bff;BffA;ArFFase) 3.2.1.26 Arthrobacter sp. K-1 BAB72022.1 Q8VW87
3VSR[A] 2.00
3VSS[A] β-D-Fruf
1.97
3WPU[A,B] 1.60
3WPV[A,B,C] 1.81
3WPY[A,B,C] 2.00
3WPZ[A,B,C] 2.27
2
 β-fructosyltransferase (BiBftA; Bind_2021) 2.4.1.10 Beijerinckia indica subsp. indica ATCC 9039 ACB95643.1 B2IF78
6M0D[A] 2.20
6M0E[A] β-D-Fruf
β-D-Frup
1.35
8I2Q[A] 2.15
8I2R[A] β-D-Fruf
β-D-Frup
1.36
2
 levansucrase (Brs-LS; EH206_19850) 2.4.1.10 Brenneria nigrifluens ATCC 13028 QCR06203.1 G7LSK3
7EHR[A] 1.33
7EHS[A] 1.60
7EHT[A] 1.45
7FDZ[A] α-D-Glcp-(1-2)-β-D-Fruf
1.35
2
 EAM_3468 (LsC) 2.4.1.10 Erwinia amylovora ATCC 49946 CBJ48143.1 A0A0M3KKU6
D4IGH9
4D47[A,B,C,D,E,F,G,H] α-D-Glcp
β-D-Fruf
2.77
2
 levansucrase (ETA_34670; LsC) 2.4.1.10 Erwinia tasmaniensis Et1/99 CAO98513.1 B2VCC3
6FRW[A] 1.52
6RV5[A] β-D-Fruf-(2-6)-β-D-Fruf
1.58
7OSO[A] 1.40
2
 levansucrase (LdsA) 2.4.1.10 Gluconacetobacter diazotrophicus SRT4 AAB36606.1 Q43998
Q5I5I3
1W18[A,B] 2.5
2
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