Glycoside Hydrolase Family 65

Known Activities	α,α-trehalase (EC 3.2.1.28); maltose phosphorylase (EC 2.4.1.8); trehalose phosphorylase (EC 2.4.1.64); kojibiose phosphorylase (EC 2.4.1.230); trehalose-6-phosphate phosphorylase (EC 2.4.1.-); nigerose phosphorylase (EC 2.4.1.-)
Mechanism	Inverting
Clan	GH-L
3D Structure Status	( α / α ) ₆
Catalytic Nucleophile/Base	phosphate for phosphorylases; water for hydrolases
Catalytic Proton Donor	Glu
External resources	CAZypedia; InterPro; PFAM;
Statistics	GenBank accession (792); Uniprot accession (430); PDB accession (1); 3D entries (2); cryst (1)

Summary

All (701) Archaea (3) Bacteria (634) Eukaryota (62) unclassified (2) Structure (2 - 1 cryst) Characterized (15)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
maltose phosphorylase (MPase)	2.4.1.8	Bacillus sp. RK-1	BAC54904.1	Q84IX5
nigerose phosphorylase (Cphy_1874)	2.4.1.-	Clostridium phytofermentans ISDg	ABX42243.1	A9KT32
trehalose phosphorylase (TPase)	2.4.1.64	Geobacillus stearothermophilus SK-1	BAC20640.1	Q8GRC3
maltose phosphorylase (MalP;LBA1870)	2.4.1.8	Lactobacillus acidophilus NCFM	AAV43670.1 CAN87417.1
maltose phosphorylase	2.4.1.8	Lactobacillus brevis		Q7SIE1	1H54[A]
maltose phosphorylase	2.4.1.8	Lactobacillus sanfranciscensis DSM 20451(T)	CAA11905.1	O87772
trehalose-6-phosphate phosphorylase (TrePP;YeeA;L39593;LL0428)	2.4.1.-	Lactococcus lactis subsp. lactis Il1403	AAK04526.1 NP_266584.1	Q9CID5.3
maltose phosphorylase (MapA)	2.4.1.8	Paenibacillus sp. SH-55	BAD97810.1 BAJ22988.1	Q50LH0
trehalose phosphorylase (TreP)	2.4.1.64	Thermoanaerobacter brockii ATCC 35047	AAE18727.1 AAE24711.1 BAB97299.1	Q8L164	cryst
kojibiose phosphorylase (KojP;KPase)	2.4.1.230	Thermoanaerobacter brockii ATCC 35047	AAE30762.1 BAB97300.1	Q8L163
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
AN9340.2 (TreA)	3.2.1.28	Aspergillus nidulans FGSC A4	EAA66407.1
acid trehalase (Atc1)	3.2.1.28	Candida albicans	AAV05390.1
acid trehalase (TreA)	3.2.1.28	Emericella nidulans	AAB57642.1	P78617
acid trehalase (Atm1)	3.2.1.28	Metarhizium acridum CQMA102	ABB51158.1 ABB51159.1 ABO93464.1
acid trehalase (vacuolar) (Ath1;YPR026w;YP9367.06)	3.2.1.28	Saccharomyces cerevisiae S288c	CAA58961.1 CAA89280.1 CAA95022.1 NP_015351.1	P48016

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.