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Glycoside Hydrolase Family 5

Activities in Family[retaining] arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-);
β-1,3-mannanase (EC 3.2.1.-);
β-rutinosidase /α-L-rhamnose-(1,6)-β-D-glucosidase (EC 3.2.1.-);
Glucomannan-specific endo-β-1,4-glucanase (EC 3.2.1.-);
Mannan exo-β-1,4-mannosidase (EC 3.2.1.-);
Steryl β-glucosidase (EC 3.2.1.104);
Endoglycosylceramidase (EC 3.2.1.123);
Chitosanase (EC 3.2.1.132);
β-D-galactofuranosidase (EC 3.2.1.146);
β-primeverosidase (EC 3.2.1.149);
Oligoxyloglucan reducing-end-specific cellobiohydrolase (EC 3.2.1.150);
Xyloglucan-specific endo-β-1,4-glucanase / endo-xyloglucanase (EC 3.2.1.151);
Endo-β-1,6-galactanase (EC 3.2.1.164);
Hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168);
β-glucosidase (EC 3.2.1.21);
β-galactosidase (EC 3.2.1.23);
β-mannosidase (EC 3.2.1.25);
Xylan β-1,4-xylosidase (EC 3.2.1.37);
Endo-β-1,3-glucanase / laminarinase (EC 3.2.1.39);
Endo-β-1,4-glucanase (EC 3.2.1.4);
β-glucosylceramidase (EC 3.2.1.45);
β-galactosylceramidase (EC 3.2.1.46);
β-N-acetylhexosaminidase (EC 3.2.1.52);
α-L-arabinofuranosidase (EC 3.2.1.55);
Exo-β-1,3-glucanase (EC 3.2.1.58);
Endo-β-1,3(4)-glucanase / lichenase-laminarinase (EC 3.2.1.6);
Lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73);
Exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74);
Endo-β-1,6-glucanase (EC 3.2.1.75);
Endo-β-1,4-mannanase (EC 3.2.1.78);
Endo-β-1,4-xylanase (EC 3.2.1.8);
Cellobiohydrolase (EC 3.2.1.91);
Mechanism Retaining e
ClanGH-A
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (30664); Uniprot accession (670); PDB accession (295); 3D entries (109); cryst (0)
Summary
Download GH5 (43911) Taxonomic display Structure (109) Characterized (622)
| 1 | 2 |
unclassified
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 Endo-1,4-β-mannosidase (fragment)   feces metagenome SJX74200.1     7
 Cellulase(EC:3.2.1.4)   feces metagenome SJX74201.1     4
 β-1,4-mannanase   feces metagenome SJX74184.1     8
 UFOVP58_148   freshwater metagenome CAB4125475.1    
 endo-β-1,4-mannanase 3.2.1.78 metagenome MCF0172482.1   8D89[A] 57
 ancestral endocellulase Cel5A (LFCA_EG) 3.2.1.4 synthetic construct     6GJF[B,C,D,E,F]
6GJF[A] 		2
 BN138_543   termite gut metagenome CCO21355.1     22
 endoglucanase (umCel5N) 3.2.1.4 uncultured microorganism ACH67609.1 B5LZ80   37
 endo-β-1,4-glucanase (cel3623) 3.2.1.4 uncultured microorganism AOA60286.1     37
 endo-β-1,4-glucanase (cel36) 3.2.1.4 uncultured microorganism AOA60287.1     2
 endo-β-1,4-glucanase (cel7482) 3.2.1.4 uncultured microorganism AOA60285.1     37
 cellodextrinase (UmCel5Q) (UmCel5Q)   uncultured microorganism ADB80112.1     38
 endoglucanase (LC2)   uncultured microorganism ADB80100.1     4
 endoglucanase (UmCel5K) (Cel5K) 3.2.1.4 uncultured microorganism ADB80108.1 D3JVT9   26
 endoglucanase (UmCel5R) (Cel5R)   uncultured microorganism ADB80110.1     26
 cellodextrinase (UmCel5P) (Cel5P)   uncultured microorganism ADB80111.1     26
 cellodextrinase (UmCel5O) (Cel5O)   uncultured microorganism ADB80113.1     38
 endoglucanase / xylanase (CelA;RuCelA) 3.2.1.73 uncultured microorganism ADK55024.1 E2DQY5   4
 BT-05   uncultured microorganism ADR64667.1     4
 BT-06   uncultured microorganism ADR64668.1     4
 cellulase (BT-01) (partial) 3.2.1.4 uncultured microorganism ADR64663.1 E5F2K8   4
 BT-02 (fragment)   uncultured microorganism ADR64664.1     4
 BT-03   uncultured microorganism ADR64665.1    
 BT-04   uncultured microorganism ADR64666.1     4
 cellulase (Umcel5B29;UmCel5B_29) 3.2.1.4 uncultured microorganism AEK98797.1 G0Z5J9   4
 cellulase (Cel5-A24) (fragment)   uncultured microorganism AEV46036.1     4
 cellulase (Cel5-A7) (fragment)   uncultured microorganism AEV46031.1     4
 cellulase (cel5-A9) (fragment)   uncultured microorganism AEV46032.1     4
 cellulase (Cel5-A12) (fragment)   uncultured microorganism AEV46033.1     4
 cellulase (Cel5-A15) (fragment)   uncultured microorganism AEV46034.1     4
 cellulase (Cel5-A22) (fragment)   uncultured microorganism AEV46035.1     4
 cellulase (Cel5-A26) (fragment)   uncultured microorganism AEV46037.1     4
 cellulase (Cel5-A27) (fragment)   uncultured microorganism AEV46038.1     4
 cellulase (Cel5-A28) (fragment)   uncultured microorganism AEV46039.1     4
 cellulase (Cel5-A32) (fragment)   uncultured microorganism AEV46040.1     4
 cellulase (Cel5_A24)   uncultured microorganism AEX97595.1     4
 cellulase (Cel5_A27)   uncultured microorganism AEX97596.1     4
 cellulase (Cel14b22) 3.2.1.4 uncultured microorganism AFQ39736.1 M4HLK8   4
 endo-β-1,4-glucanase (CelZ)   uncultured microorganism AHB33631.1     4
 cellulase (Cel28A) 3.2.1.4 uncultured microorganism AHW46443.1 X5CIW4   4
 β-glucanase (RuCelA;C29-2) 3.2.1.4 uncultured microorganism ACA61132.1 B1PLH5   4
 β-glucanase (C35-2) 3.2.1.4 uncultured microorganism ACA61135.1 B1PLH8   4
 β-glucanase (C67-1) 3.2.1.4 uncultured microorganism ACA61137.1 B1PLI0   4
 β-glucanase (C5614-1) 3.2.1.4 uncultured microorganism ACA61140.1 B1PLI3   4
 cellulase (DC2-3)   uncultured microorganism ACA61144.1     4
 β-glucanase (DC2-4) 3.2.1.4 uncultured microorganism ACA61145.1 B1PLI8   4
 β-glucanase (DC3-1) 3.2.1.4 uncultured microorganism ACA61149.1 B1PLJ2   4
 cellulase (DC9-2) 3.2.1.4 uncultured microorganism ACA61152.1 B1PLJ5   26
 β-glucanase (DC20-2) 3.2.1.73 uncultured microorganism ACA61156.1 B1PLJ9   26
 β-glucanase (DC23-3) 3.2.1.73 uncultured microorganism ACA61160.1 B1PLK3   4
 cellulase (DM1-1)   uncultured microorganism ACA61162.1     37
 β-glucanase (M8-2) 3.2.1.73 uncultured microorganism ACA61165.1 B1PLK8   37
 β-glucanase (M11-2) 3.2.1.4 uncultured microorganism ACA61168.1 B1PLL1   26
 β-glucanase (M40-2)   uncultured microorganism ACA61171.1     38
 endoglucanase   uncultured organism ANS70888.1     4
 cellulase CM4   uncultured organism ARI46376.1     2
 cellulase CM3   uncultured organism ARI46375.1     4
 endo-β-1,4-glucanase (G08-17) (fragment) 3.2.1.4 uncultured organism ACY24829.1 D8VN44   26
 endo-β-1,4-glucanase (G10-10) 3.2.1.4 uncultured organism ACY24859.1 D8VN74   2
 ORF   uncultured organism ADD61406.1
ADD62011.1		     7
 ORF   uncultured organism ADD61407.1
ADD62012.1		     2
 ORF   uncultured organism ADD61482.1
ADD61488.1		     21
 β-glucanase 3.2.1.6 uncultured organism ADD61786.1 D9ZEN2   37
 β-glucanase 3.2.1.6 uncultured organism ADD61809.1 D9ZEQ5   4
 β-glucanase 3.2.1.4 uncultured organism ADD61853.1 D9ZEU9   1
 β-glucanase 3.2.1.4 uncultured organism ADD61911.1 D9ZF07   4
 ORF   uncultured organism ADD61973.1     4
 ORF   uncultured organism ADD61974.1    
 multimodular β-glucanase   uncultured organism ADD61840.1     2
 SARM_0071 / 181694_69520 / TX-1 3.2.1.4 uncultured organism ADX05742.1 E9NSP7   4
 SARM_0068 / 3004993_158220 / TW-3 3.2.1.4 uncultured organism ADX05739.1 E9NSP4   37
 SARM_0054 / 1557092_108510 / MH-18 3.2.1.4 uncultured organism ADX05725.1 E9NSN0   38
 SARM_0050 / 1194088_27810 / TW-48   uncultured organism ADX05721.1     4
 SARM_0047 / 1057205_158590 / TW-15 3.2.1.4 uncultured organism ADX05718.1 E9NSM3  
 SARM_0046 / 1029836_111030 / MH-2 3.2.1.73 uncultured organism ADX05717.1 E9NSM2   38
 SARM_0034 / 694713_55880 / TW-39 3.2.1.4 uncultured organism ADX05705.1 E9NSL0   4
 SARM_0032 / 558318_19410 / MH-20 3.2.1.4 uncultured organism ADX05703.1 E9NSK8   4
 SARM_0027 / 458803_07710 / TW-20   uncultured organism ADX05698.1     4
 SARM_0026 / 457020_01700 / TW-40 3.2.1.4 uncultured organism ADX05697.1 E9NSK2 5UHX[A]
5WH8[A] 		2
 SARM_0026 / 457020_01700 / TW-40   uncultured organism ADX05697.1 E9NSK2  
 endo-β-1,4-glucanase (SARM_0025;421339_68070;TW-2) 3.2.1.4 uncultured organism ADX05696.1 E9NSK1   46
 SARM_0017 / 3953955_161040 / TW-49 3.2.1.4 uncultured organism ADX05688.1 E9NSJ3   4
 SARM_0013 / 3932955_213080 / TW-1 3.2.1.4 uncultured organism ADX05684.1 E9NSI9   2
 SARM_0086 / 0_06533 / TW-18 (fragment)   uncultured organism ADX05761.1    
 SARM_0084 / 0_04358 / TW-56 (fragment; probable frameshift) 3.2.1.4 uncultured organism ADX05760.1 E9NSR5   4
 SARM_0083 / 0_01399 / MH-11 (fragment; probable frameshift)   uncultured organism ADX05757.1     52
 endo-β-1,4-glucanase (SARM_0085 / 0_04975 / TW-16) 3.2.1.4 uncultured organism ADX05752.1 E9NSQ7   52
 SARM_0081 / 0_14031 / TW-4 3.2.1.4 uncultured organism ADX05751.1 E9NSQ6   26
 endo-β-1,3(4)-glucanase (SARM_0069 / 3271578_13460 / TW-19) 3.2.1.6 uncultured organism ADX05740.1 E9NSP5   26
 endo-β-1,4-glucanase(SARM_0066 / 2698429_129360 / TW-22) 3.2.1.4 uncultured organism ADX05737.1 E9NSP2   52
 SARM_0063 / 2527869_58630 / TW-33 3.2.1.4 uncultured organism ADX05734.1 A0A076MPD7
E9NSN9		 4YHG[A,B] 4
 SARM_0061 / 2463295_41230 / MH-3 3.2.1.4 uncultured organism ADX05732.1 E9NSN7   26
 SARM_0058 / 2395619_81340 / TW-44 3.2.1.4 uncultured organism ADX05729.1 E9NSN4   4
 SARM_0055 / 1696514_56150 / TW-29 (fragment)   uncultured organism ADX05726.1     2
 SARM_0038 / 798985_48230 / TW-11   uncultured organism ADX05709.1     8
 SARM_0004 / 3671981_90060 / MH-13 (fragment)   uncultured organism ADX05675.1     38
 CEL-5M GH5 cellulase   uncultured organism QFQ13830.1     2
 cellulase 5K (Cel5K) 3.2.1.4 uncultured organism AEC45566.1 F5CT74   1
 cellulase (CelEx-BR12) 3.2.1.4
3.2.1.8		 uncultured organism AGK74985.1 N0CH57   38
 endoglucanase (CelH) (fragment)   uncultured organism WPA94121.1     25
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