Glycoside Hydrolase Family 5 / Subf 9

Activities in Sub Family	β-glucosidase (EC 3.2.1.21); Exo-β-1,3-glucanase (EC 3.2.1.58); Endo-β-1,6-glucanase (EC 3.2.1.75);
Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (3626); Uniprot accession (17); PDB accession (13); 3D entries (2); cryst (0)

Eukaryota

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

Subf

exo-β-1,3-glucanase (Xog; XOG1; Exg; Ca49c10.05)

CAA39908.1
AAV05307.1
CAA21969.1

1CZ1[A]		1.85
1EQC[A]	<carb_like_CTS>	1.85
1EQP[A]		1.90
2PB1[A]	Î±-D-Glcp2F-(1-4)-GLU Î²-D-Glcp2F-(1-1)-<non_carb>	1.90
2PBO[A]		1.85
2PC8[A]	Î²-D-Glcp	1.80
2PF0[A]		1.90
3N9K[A]	Î²-D-Glcp-(1-3)-Î²-D-Glcp Î²-D-Glcp-(1-3)-Î²-D-Glcp-(1-3)-Î²-D-Glcp	1.70
3O6A[A]		2.00
4M80[A]		1.86
4M81[A]	Î±-D-Glcp1F Î²-D-Glcp-(1-1)-<non_carb>	1.86
4M82[A]	Î²-D-Glcp Î²-D-Glcp-(1-6)-Î²-D-Glcp-(1-1)-<non_carb>	1.59

exo-β-1,3-glucanase 1 / flavonoid β-glucosidase (Exg1;Bgl1;Scw6;YLR300W)

AAA34599.1
AAB67345.1
AAT93088.1
NP_013403.1

1.75