Glycoside Hydrolase Family 5 / Subf 7

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (504); Uniprot accession (158); PDB accession (26); 3D entries (10); cryst (0)
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 β-mannosidase 5A (Man5A) 3.2.1.25 Cellvibrio mixtus NCIMB 8633 AAS19695.1 Q6QT42
1UUQ[A] 1.5
1UZ4[A] <carb_like_IFL>
1.71
7
 endo-β-1,4-mannanase (Man;TpMan;TpManGH5;Tpet_1542) 3.2.1.78 Thermotoga petrophila RKU-1 ABQ47550.1
YP_001245126.1
A5IMX7
3PZ9[A] 1.42
3PZG[A] 1.40
3PZI[A] β-D-Glcp
1.55
3PZM[A] 1.50
3PZN[A] 1.50
3PZO[A] α-D-Glcp-(1-4)-α-D-Glcp
1.55
3PZQ[A] α-D-Glcp-(1-4)-α-D-Glcp
1.92
7
Eukaryota
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 endo-β-1,4-mannanase (ManBK) (partial) 3.2.1.78 Aspergillus niger BK 01 ACJ06979.1 B6V876
3WH9[A,B] 1.57
7
 endo-β-1,4-mannanase (Man5) (partial)   Neurospora sitophila CCI55471.1  
4AWE[A] 1.40
7
 endo-β-1,4-mannanase (PaMan5A;Pa_6_490) (Man5A) 3.2.1.78 Podospora anserina S mat+ CAP71606.1
ADO14134.1
CDP31001.1
B2B3C0
3ZIZ[A] 1.40
7
 β-mannanase (RmMan5B) (Man5B) 3.2.1.78 Rhizomucor miehei CAU432 AGV01048.1  
4LYP[A,B] 1.28
4LYQ[A] β-D-Manp-(1-4)-β-D-Manp-(1-4)-α-D-Lyxp
2.00
4LYR[A] 2.50
4NRR[A,B] β-D-Manp-(1-4)-β-D-Fruf
2.40
4NRS[A,B] β-D-Manp-(1-4)-α-D-Manp
2.57
7
 β-mannanase (RmMan5A) (Man5A) 3.2.1.78 Rhizomucor miehei CAU432 / CGMCC 4967 AGC24277.1  
4L3M[A] 2.26
4QP0[A] 2.30
7
 endo-β-mannanase 4a / mannan transglycosylase/hydrolase (Man4;LeMan4;LeMAN4a;LeMAN4i;MTH) 3.2.1.78
2.4.1.-
Solanum lycopersicum AAK97760.1
AAK56557.1
AAK97759.2
Q8L5J1
Q8RVL3
Q93WT4
1RH9[A] 1.50
7
 β-mannanase 3.2.1.78 Talaromyces trachyspermus ATCC 24792 BAP19029.1  
3WFL[A] 1.60
7
 β-mannanase (TrMan5A) (Man5A) 3.2.1.78 Trichoderma reesei RUTC-30 AAA34208.1
ABJ41268.1
Q99036
1QNO[A] 2.0
1QNP[A] 1.5
1QNQ[A] 1.65
1QNR[A] β-D-Manp-(1-4)-β-D-Manp
1.4
1QNS[A] 1.5
7
Top




Last update: 2017-10-09 © Copyright 1998-2017
AFMB - CNRS - Université d'Aix-Marseille