Activities in Sub Family	β-mannosidase (EC 3.2.1.25); Endo-β-1,4-mannanase (EC 3.2.1.78);
Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (1545); Uniprot accession (72); PDB accession (28); 3D entries (10); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
β-mannosidase 5A (Man5A) (Man5A)	3.2.1.25	Cellvibrio mixtus NCIMB 8633	AAS19695.1	Q6QT42	1UUQ[A] 1.5 1UZ4[A] <carb_like_IFL> 1.71 7ODJ[A]	7
endo-β-1,4-mannanase (Man;TpMan;TpManGH5;Tpet_1542)	3.2.1.78	Thermotoga petrophila RKU-1	ABQ47550.1 YP_001245126.1	A5IMX7	3PZ9[A] 1.42 3PZG[A] 1.40 3PZI[A] β-D-Glcp 1.55 3PZM[A] 1.50 3PZN[A] 1.50 3PZO[A] α-D-Glcp-(1-4)-α-D-Glcp 1.55 3PZQ[A] α-D-Glcp-(1-4)-α-D-Glcp 1.92 6TN6[A] 1.45	7
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
endo-β-1,4-mannanase (ManBK) (partial)	3.2.1.78	Aspergillus niger BK 01	ACJ06979.1	B6V876	3WH9[A,B] 1.57	7
endo-β-1,4-mannanase (Man5; CsMan5) (partial)	3.2.1.78	Neurospora sitophila DSM 16514	CCI55471.1		4AWE[A] 1.40	7
endo-β-1,4-mannanase (PaMan5A;Pa_6_490) (Man5A)	3.2.1.78	Podospora anserina S mat+	CAP71606.1 ADO14134.1 CDP31001.1	B2B3C0	3ZIZ[A] 1.40	7
β-mannanase (RmMan5B) (Man5B)	3.2.1.78	Rhizomucor miehei CAU432	AGV01048.1	A0A075C6T6	4LYP[A,B] 1.28 4LYQ[A] β-D-Manp-(1-4)-β-D-Manp-(1-4)-α-D-Lyxp 2.00 4LYR[A] 2.50 4NRR[A,B] β-D-Manp-(1-4)-β-D-Fruf 2.40 4NRS[A,B] β-D-Manp-(1-4)-α-D-Manp 2.57	7
β-mannanase (RmMan5A) (Man5A)	3.2.1.78	Rhizomucor miehei CAU432 / CGMCC 4967	AGC24277.1	L7SVX1	4L3M[A] 2.26 4QP0[A] 2.30	7
endo-β-mannanase 4a / mannan transglycosylase/hydrolase (Man4; LeMan4; LeMAN4a; LeMAN4i; MTH)	3.2.1.78	Solanum lycopersicum	AAK97760.1 AAK56557.1 AAK97759.2	Q8L5J1 Q8RVL3 Q93WT4	1RH9[A] 1.50	7
β-mannanase	3.2.1.78	Talaromyces trachyspermus ATCC 24792	BAP19029.1	A0A077JNB5 A0A087WNH1	3WFL[A] 1.60	7
β-mannanase (Man1; TrMan5A) (Man5A)	3.2.1.78	Trichoderma reesei RUTC-30	AAA34208.1 ABJ41268.1	Q99036	1QNO[A] 2.0 1QNP[A] 1.5 1QNQ[A] 1.65 1QNR[A] β-D-Manp-(1-4)-β-D-Manp 1.4 1QNS[A] 1.5	7
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