Glycoside Hydrolase Family 5 / Subf 5

Activities in Sub Familyendo-b-1,4-glucanase (EC 3.2.1.4)
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (6654); Uniprot accession (60); PDB accession (30); 3D entries (9); cryst (0)
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 endo-β-1,4-glucanase (Cel5A ; Ps_Cel5A; PST_2494) 3.2.1.4 Pseudomonas stutzeri A1501 ABP80146.1 A4VME5
4LX4[A,B,C,D] 1.56
6R2J[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp
1.44
5
 endoglucanase (RBcel1) 3.2.1.4 uncultured bacterium ACO55737.1 C1JI15
4EE9[A] 1.38
4M24[A] β-D-Glcp-(1-4)-β-D-Glcp
1.39
5LJF[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.73
6ZZ3[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU
2.10
7P6G[A] 1.49
7P6H[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
1.73
7P6I[A] 1.47
7P6J[A] β-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU
1.75
5
 XAC0029   Xanthomonas citri pv. citri str. 306 AAM34921.1
NP_640385.1
 
5HOS[A] 1.60
5
 XAC0030 (Egl)   Xanthomonas citri pv. citri str. 306 AAM34922.1
NP_640386.1
 
4W7U[A] 1.48
4W7V[A] β-D-Glcp-(1-4)-β-D-Glcp
1.43
4W7W[A] β-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp
1.05
5HNN[A,B,C] 1.63
5HPC[A] 2.60
5
Eukaryota
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 endo-β-1,4-glucanase (Eng1;EgI;AnCel5A) 3.2.1.4 Aspergillus niger IFO31125 AAG50051.1 O74706
Q9C3Z7
5I77[A] β-D-GlcpNAc
1.80
5I78[A,B] 1.58
5I79[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.35
5
 β-1,4-endoglucanase (GlCel5A;GL28282) (Cel5A) 3.2.1.4 Ganoderma lucidum   A0A1A9TAJ1
5D8W[A,B] 2.86
5D8Z[A,B] β-D-Glcp-(1-4)-β-D-Glcp
2.70
5
 endoglucanase 2 (EG2;EGIIa) (partial) (Cel5A) 3.2.1.4 Talaromyces verruculosus B221-151   A0A1U7Q1U3
5L9C[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp
1.85
6T9F[A,B,C,D] 2.25
6T9G[A,B,C,D] 2.30
6TPC[A,C] 1.52
6YON[A,B,C,D] 2.60
5I6S[A] 2.10
5
 endo-β-1,4-glucanase (Eg1;Eng1;TaCel5A) (Cel5A) 3.2.1.4 Thermoascus aurantiacus IFO 9748 AAL88714.2
AAL16412.1
ACE10214.1
CAT02251.1
CAZ67882.1
Q8TG26
Q96UI5
1GZJ[A,B] 1.62
1H1N[A,B] 1.12
5
 endo-β-1,4-glucanase II (EG2;EGII;Egl2;EG-II;TrEGII;TrCel5A) (formerly EGIII) (Cel5A) 3.2.1.4 Trichoderma reesei AAA34213.1
AAQ24883.1
AAR60515.1
ABA64553.1
ABE22094.1
P07982
Q2F8H3
3QR3[A,B] 2.05
5
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