Glycoside Hydrolase Family 5 / Subf 53

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (20); Uniprot accession (7);
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 glycosyl hydrolase family 5_53 domain-containing protein   Alteromonadaceae bacterium Bs02 AIF91557.1     53
 glycosyl hydrolase family 5_53 domain-containing protein   Alteromonadaceae bacterium Bs08 AIF91548.1     53
 glycosyl hydrolase family 5_53 domain-containing protein   Alteromonadaceae bacterium Bs08 AIF91545.1     53
 glycosyl hydrolase family GH5_53 domain-containing protein   Alteromonadaceae bacterium Bs12 AIF91526.1     53
 glycosyl hydrolase family 5_53 domain-containing protein   Alteromonadaceae bacterium Bs12 AIF91542.1     53
 glycosyl hydrolase family 5_53 domain-containing protein   Alteromonadaceae bacterium Bsc2 AIF91521.1     53
 cellodextrinase C / 5A (CelC;CJA_1462) (Cel5A) 3.2.1.74 Cellvibrio japonicus Ueda107 ACE82870.1
CAA43597.1
B3PDK2
P27033
  53
 CBR65_03680   Cellvibrio sp. PSBB006 ARU26597.1     53
 B0D95_07975   Cellvibrio sp. PSBB023 AQT60034.1     53
 YC6258_02765   Gynuella sunshinyii YC6258 AJQ94803.1     53
 YC6258_01195   Gynuella sunshinyii YC6258 AJQ93243.1     53
 A3224_13335 (fragment)   Microbulbifer thermotolerans DAU221 AMX04259.1     53
 cellulase (Cel;Cell JV)   Pseudomonas fluorescens ADK13057.1 D9J046   53
 endoglucanase 5I (Sde_3420) (Cel5I)   Saccharophagus degradans 2-40 ABD82675.1 Q21F54   53
 sce5351 (CelC)   Sorangium cellulosum So ce56 CAN95514.1 A9FXH1   53
 SCE1572_20220   Sorangium cellulosum So0157-2 AGP36616.1     53
 TERTU_0428   Teredinibacter turnerae T7901 ACR12792.1 C5BMU2   53
 TERTU_0427   Teredinibacter turnerae T7901 ACR13327.1 C5BMU1   53
 BSQ33_03035   Vibrio gazogenes ATCC 43942 ASA54802.1     53
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