Glycoside Hydrolase Family 5 / Subf 32

Activities in Sub FamilyNo known activity in this subfamily.
Activities in Family:
[retaining] b-1,4-mannan transglycosylase (EC 2.4.1.-);[retaining] arabinoxylan-specific endo-b-1,4-xylanase (EC 3.2.1.-);[retaining] exo-xyloglucanase (EC 3.2.1.-);b-1,3-mannanase (EC 3.2.1.-);b-glycosidase (EC 3.2.1.-);b-rutinosidase /a-L-rhamnose-(1,6)-b-D-glucosidase (EC 3.2.1.-);glucomannan-specific endo-b-1,4-glucanase (EC 3.2.1.-);exo-b-1,4-mannobiohydrolase / mannobiose-producing exo-b-mannanase (EC 3.2.1.100);steryl b-glucosidase (EC 3.2.1.104);endoglycosylceramidase (EC 3.2.1.123);chitosanase (EC 3.2.1.132);b-D-galactofuranosidase (EC 3.2.1.146);b-primeverosidase (EC 3.2.1.149);xyloglucan-specific endo-b-1,4-glucanase / endo-xyloglucanase (EC 3.2.1.151);endo-b-1,6-galactanase (EC 3.2.1.164);hesperidin 6-O-a-L-rhamnosyl-b-glucosidase (EC 3.2.1.168);b-glucosidase (EC 3.2.1.21);b-galactosidase (EC 3.2.1.23);b-mannosidase (EC 3.2.1.25);xylan b-1,4-xylosidase (EC 3.2.1.37);endo-b-1,3-glucanase / laminarinase (EC 3.2.1.39);endo-b-1,4-glucanase (EC 3.2.1.4);b-glucosylceramidase (EC 3.2.1.45);b-galactosylceramidase (EC 3.2.1.46);b-N-acetylhexosaminidase (EC 3.2.1.52);a-L-arabinofuranosidase (EC 3.2.1.55);exo-b-1,3-glucanase (EC 3.2.1.58);endo-b-1,3(4)-glucanase / lichenase-laminarinase (EC 3.2.1.6);lichenase / endo-b-1,3-1,4-glucanase (EC 3.2.1.73);exo-b-1,4-glucanase / cellodextrinase (EC 3.2.1.74);endo-b-1,6-glucanase (EC 3.2.1.75);endo-b-1,4-mannanase (EC 3.2.1.78);endo-b-1,4-xylanase (EC 3.2.1.8)
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (6); Uniprot accession (0);




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