Glycoside Hydrolase Family 5 / Subf 25

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (155); Uniprot accession (23); PDB accession (16); 3D entries (3); cryst (0)
| 1 | 2 |
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 CDL62_01845   Alkalitalea saponilacus DSM 24412 ASB47982.1     25
 ANT_18750   Anaerolinea thermophila UNI-1 BAJ63901.1     25
 SAMN00777080_1515   Aquiflexum balticum DSM 16537 SMD42944.1     25
 Astex_2497   Asticcacaulis excentricus CB 48 ADU14148.1 C5SQ05   25
 NK6_7446   Bradyrhizobium diazoefficiens NK6 BAR60597.1     25
 AAV28_13865   Bradyrhizobium diazoefficiens USDA 110 AND88774.1     25
 blr3367   Bradyrhizobium diazoefficiens USDA 110 NP_770007.1
BAC48632.1
Q89PW3   25
 BD122_29925   Bradyrhizobium diazoefficiens USDA 122 APO54584.1     25
 RN69_31130   Bradyrhizobium japonicum E109 AJA64257.1     25
 BKD09_RS33400   Bradyrhizobium japonicum J5 APG13256.1     25
 BJS_06698   Bradyrhizobium japonicum SEMIA 5079 AHY51746.1     25
 BJ6T_64240   Bradyrhizobium japonicum USDA 6 BAL11673.1     25
 BN2626_G22_6460   Bradyrhizobium sp. CUU20044.1     25
 BF49_0114   Bradyrhizobium sp. CUT09034.1     25
 BCCGELA001_14935   Bradyrhizobium sp. CCGE-LA001 AMA57428.1     25
 Cabys_1971   Caldithrix abyssi DSM 13497 LF13 APF18720.1     25
 Cseg_2049   Caulobacter segnis ATCC 21756 ADG10516.1 D5VGS3   25
 B7G68_10495 (fragment)   Caulobacter segnis TK0059 AVQ04414.1     25
 Caul_1283 (Cel5A) 3.2.1.4 Caulobacter sp. K31 ABZ70413.1 B0SYT2   25
 ATE48_16720   Caulobacteraceae bacterium OTSz_A_272 ANP47437.1     25
 BN1096_520152   Clostridioides difficile CDS85201.1     25
 C3348_13235   Clostridioides difficile 08-00495 AVB50523.1     25
 TW87_15900   Clostridioides difficile 08ACD0030 AMM58636.1     25
 C3353_13230   Clostridioides difficile 09-00072 AVB61456.1     25
 C3349_13235   Clostridioides difficile 10-00071 AVB57828.1     25
 C3351_13240   Clostridioides difficile 10-00078 AVB65027.1     25
 C3352_13240   Clostridioides difficile 10-00253 AVB68617.1     25
 C3347_13230   Clostridioides difficile 12-00008 AVB34576.1     25
 C3350_13245   Clostridioides difficile 12-00011 AVB54202.1     25
 CD2546   Clostridioides difficile 630 CAJ69433.1
AJP12293.1
Q182N3   25
 CDIF630erm_02798   Clostridioides difficile 630 delta erm ARE63472.1     25
 CD630DERM_25460   Clostridioides difficile 630Derm CEJ99169.1     25
 BN1095_620120   Clostridioides difficile 7032989 CDT65567.1     25
 BN1097_680032   Clostridioides difficile 7032994 CDS88444.1     25
 C4J70_12430   Clostridioides difficile AK AVI13053.1     25
 CDIF1296T_02673   Clostridioides difficile ATCC 9689 = DSM 1296 AKP43506.1     25
 BZ168_05215   Clostridioides difficile BR81 AQU09054.1     25
 C3W74_13230   Clostridioides difficile CD-10-00484 AVB41920.1     25
 CD196_2386   Clostridioides difficile CD196 CBA64573.1 C9XMR5   25
 CGC51_13185   Clostridioides difficile DH/NAP11/106/ST-42 ASN90242.1     25
 A6J95_16270   Clostridioides difficile FDAARGOS_267 ARC16276.2     25
 ERS445050_02541 (CelH)   Clostridioides difficile NCTC13307 CKH36269.1     25
 CWR57_12555   Clostridioides difficile R0104a AUA37788.1     25
 C4E25_05975   Clostridioides difficile R1 AVD42455.1     25
 C4E26_05970   Clostridioides difficile R2 AVD38929.1     25
 CDR20291_2433   Clostridioides difficile R20291 CBE05753.1 C9YPB9   25
 C3L34_13715   Clostridioides difficile R20291 AVB38292.1     25
 C4E42_18635   Clostridioides difficile R3 AVD37620.1     25
 CWR54_12590   Clostridioides difficile W0003a AUA29857.1     25
 CWR55_12800   Clostridioides difficile W0022a AUA22565.1     25
 CWR56_12870   Clostridioides difficile W0023a AUA26314.1     25
 PCZ31_1913 (CelH)   Clostridioides difficile Z31 ALP03840.1     25
 NPD4_734   Clostridium butyricum CDC_51208 APF24128.1     25
 ATD26_15375   Clostridium butyricum JKY6D1 ALS18212.1     25
 ATN24_16655   Clostridium butyricum KNU-L09 ALP91715.1     25
 BBB49_14670   Clostridium butyricum TK520 AOR95284.1     25
 AZ909_15110   Clostridium butyricum TOA ANF15335.1     25
 endo-β-1,4-glucanase D (CelD;CelCCD;EGCCD;Ccel_0840) (Cel5D) 3.2.1.4 [Clostridium] cellulolyticum H10 ACL75216.1
BAA14354.1
P25472.1
P25472   25
 A9D14_07150   Croceicoccus marinus E4A9 ARU16008.1     25
 AEP_03465 (CelH)   Curvibacter sp. AEP1-3 ARV20385.1     25
 SAMN06298216_1039   Cytophagales bacterium TFI 002 SOE20551.1     25
 DICTH_0008 (Cel5A) 3.2.1.4 Dictyoglomus thermophilum H-6-12 ACI18520.1 B5YAS2   25
 DICTH_0506   Dictyoglomus thermophilum H-6-12 ACI19154.1 B5YCY0   25
 DICTH_0505   Dictyoglomus thermophilum H-6-12 ACI19692.1 B5YCX9   25
 cellulase (Dtur_0276)   Dictyoglomus turgidum DSM 6724 ACK41602.1 B8DYX6   25
 endoglucanase (LC1;CelA;Dtur_0670) (Cel5B) 3.2.1.4 Dictyoglomus turgidum DSM 6724 ACK41955.1 B8DZM2   25
 Dtur_0669   Dictyoglomus turgidum DSM 6724 ACK41954.1 B8DZM1   25
 endoglucanase (FgCel5A;Cel14.1) (Cel5A) 3.2.1.4 Fervidobacterium gondwanense AB39 AFY97404.1     25
 NA23_08975   Fervidobacterium islandicum AW-1 AMW33355.1     25
 endo-β-1,4-glucanase (FnCel5A;Fnod_1560) (Cel5A) 3.2.1.4 Fervidobacterium nodosum Rt17-B1 ABS61403.1
ADD73709.1
A7HNC0 3NCO[A,B]
3RJX[A]
3RJY[A]
25
 Ferpe_1841   Fervidobacterium pennivorans DSM 9078 AFG35892.1     25
 JM64_06420   Fervidobacterium pennivorans DYC ANE42311.1     25
 MY04_5155   Flammeovirga sp. MY04 ANQ52487.1     25
 J421_5858   Gemmatirosa kalamazoonesis KBS708 AHG93393.1     25
 Hbal_1973   Hirschia baltica ATCC 49814 ATCC 59814 ACT59657.1 C6XKU0   25
 AEM38_08310   Hyphomonadaceae bacterium UKL13-1 AMS30920.1     25
 Isop_0323   Isosphaera pallida ATCC 43644 ADV60918.1     25
 IX53_01755   Kosmotoga pacifica SLHLJ1 AKI98197.1     25
 Mahau_0466   Mahella australiensis 50-1 BON AEE95680.1     25
 SAMN04489810_2829   Microbacterium pygmaeum DSM 23142 SDH33403.1     25
 A7982_03354   Minicystis rosea DSM 24000 APR78007.1     25
 SAMN05216490_2516   Mucilaginibacter mallensis MP1X4 SDT11367.1     25
 RG1141_CH32600   Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI 1141 CDN55595.1     25
 RG540_CH33430   Neorhizobium galegae bv. orientalis str. HAMBI 540 CDN49507.1     25
 Cel5A   Paenibacillus curdlanolyticus B-6 AKP16689.1     25
 B9T62_03945   Paenibacillus donghaensis KCTC 13049 ASA20023.1     25
 BSF38_02917   Paludisphaera borealis PX4 APW61403.1     25
 Psesu_1585   Pseudoxanthomonas suwonensis 11-1 ADV27430.1     25
 UC35_16420   Ramlibacter tataouinensis 5-10 AMO25510.1     25
 Rta_10360   Ramlibacter tataouinensis TTB310 AEG92121.1     25
 RB5256   Rhodopirellula baltica SH 1 CAD78375.1
NP_866594.1
Q7UGF4   25
 Rpdx1_4768   Rhodopseudomonas palustris DX-1 ADU46314.1 D2M8H1   25
 AD2_02267   Ruminiclostridium thermocellum AD2 ALX09255.1     25
 bifunctional endo-β-1,4-glucanase / β-1,4-xylanase H (CelH;EgH;CtCelH;CtCel5E;Cthe_1472) (Lic26A;Cel5E) 3.2.1.4
3.2.1.8
Ruminiclostridium thermocellum ATCC 27405 ABN52701.1
CCF82162.1
P16218 4U3A[A,B]
4U5I[A,B]
4U5K[A,B]
5BYW[A,B,C,D,E]
25
 Clo1313_2234   Ruminiclostridium thermocellum DSM 1313 ADU75267.1     25
 LQRI_2266   Ruminiclostridium thermocellum DSM 2360 LQRI ANV77007.1     25
 endo-β-1,4-glucanase / β-1,3:1,4-glucanase H (CelH) (Lic26A;Cel5E) 3.2.1.4
3.2.1.73
Ruminiclostridium thermocellum NCIB 10682 AAA23225.1 H6SHY4
P16218
  25
 C1T17_01980   Sphingobium sp. SCG-1 AUW60024.1     25
 C1T17_03720 (fragment)   Sphingobium sp. SCG-1 AUW60059.1     25
 MC45_17220   Sphingomonas taxi ATCC 55669 AIT08352.1     25
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