Glycoside Hydrolase Family 5 / Subf 1

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (510); Uniprot accession (102); PDB accession (15); 3D entries (3); cryst (0)
Archaea
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 endo-β-1,4-glucanase (EGPh;EglB;TCel3;PH1171) 3.2.1.4 Pyrococcus horikoshii OT3 AAQ31833.1
AAQ31832.1
BAA30271.1
NP_143072.1
O58925
2ZUM[A] 1.95
2ZUN[A,B,C] β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU
β-D-Glcp-(1-4)-β-D-Glcp
2.00
3AXX[A,B,C] β-D-Glcp-(1-4)-β-D-Glcp
1.90
3QHM[A,B,C] β-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.01
3QHN[A,B,C] β-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.99
3QHO[A,B,C] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.65
3VVG[A,B,C] 1.90
3W6L[A,B,C] 1.75
3W6M[A,B,C] 1.95
4DM1[A,B,C] 1.75
4DM2[A,B,C] 1.95
1
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 endo-β-1,4-glucanase E1 (EGAc;EGI;TcE1;Acel_0614) (Cel5A) 3.2.1.4 Acidothermus cellulolyticus 11B AAA75477.1
ABH84883.1
ABK52387.1
ACW33392.1
CAD42489.1
CAR96693.1
P54583
1C0D[A,B] 2.40
1ECE[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.40
1VRX[A,B] 2.40
1
 endo-β-1,4-glucanase (EngXCA;EngA) (frameshift) 3.2.1.4 Xanthomonas campestris pv. campestris CAC18529.1
AAA27612.1
Q9EVR6
4TUF[A,B,C,D] 2.70
1
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