Activities in Sub Family	Endo-β-1,4-glucanase (EC 3.2.1.4); Lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); Exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74);
Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (60); Uniprot accession (36); PDB accession (16); 3D entries (4); cryst (0)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
endo-β-1,4-glucanase (EGPh;EglB;TCel3;PH1171)	3.2.1.4	Pyrococcus horikoshii OT3	AAQ31833.1 AAQ31832.1 BAA30271.1 NP_143072.1	O58925	2ZUM[A] 1.95 2ZUN[A,B,C] β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU β-D-Glcp-(1-4)-β-D-Glcp 2.00 3AXX[A,B,C] β-D-Glcp-(1-4)-β-D-Glcp 1.90 3QHM[A,B,C] β-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 2.01 3QHN[A,B,C] β-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.99 3QHO[A,B,C] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.65 3VVG[A,B,C] 1.90 3W6L[A,B,C] 1.75 3W6M[A,B,C] 1.95 4DM1[A,B,C] 1.75 4DM2[A,B,C] 1.95	1
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
endo-β-1,4-glucanase E1 (EGAc;EGI;TcE1;Acel_0614) (Cel5A)	3.2.1.4	Acidothermus cellulolyticus 11B	AAA75477.1 ABH84883.1 ABK52387.1 ACW33392.1 CAD42489.1 CAR96693.1	P54583	1C0D[A,B] 2.40 1ECE[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 2.40 1VRX[A,B] 2.40	1
endo-β-1,4-glucanase (CelE2, partial)	3.2.1.4	uncultured bacterium	ANN44164.1		8FC0[A,B,C,D,E,F,G,H] 2.10	1
endo-β-1,4-glucanase (EngXCA;EngA) (frameshift)	3.2.1.4	Xanthomonas campestris pv. campestris	CAC18529.1 AAA27612.1	P19487 Q9EVR6	4TUF[A,B,C,D] 2.70	1
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