Glycoside Hydrolase Family 5 / Subf 1

Activities in Sub Family	Endo-β-1,4-glucanase (EC 3.2.1.4); Lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); Exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74);
Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (57); Uniprot accession (40); PDB accession (16); 3D entries (4); cryst (0)

Summary

Download GH5_1 (1604) Taxonomic display Structure (4) Characterized (38)


Archaea
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
endo-β-1,4-glucanase (EglB;TCel4;PAB0632)	3.2.1.4	pubmed	Pyrococcus abyssi GE5	CAB49854.1 AEG76944.1 NP_126623.1	Q9V052		1
endo-β-1,4-glucanase (EGPh;EglB;TCel3;PH1171)	3.2.1.4	pubmed	Pyrococcus horikoshii OT3	BAA30271.1 NP_143072.1	O58925	2ZUM[A] 2ZUN[A,B,C] 3AXX[A,B,C] 3QHM[A,B,C] 3QHN[A,B,C] 3QHO[A,B,C] 3VVG[A,B,C] 3W6L[A,B,C] 3W6M[A,B,C] 4DM1[A,B,C] 4DM2[A,B,C]	1
multidomain glycosidase (MDG; ADU37_CDS22600)	3.2.1.4	pubmed	Thermococcus sp. 2319x1	ALV63957.1	A0A0U3SGP7		1
endo-β-1,4-glucanase (Cel5A; Vul_Cel5A)	3.2.1.4	pubmed	uncultured archaeon	AZP54316.1	A0A3Q9CZQ1		1
Bacteria
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
Cthe_0405 (CelL)	3.2.1.4	pubmed	Acetivibrio thermocellus ATCC 27405	ABN51643.1	A3DCG4		1
endo-β-1,4-glucanase B (CelB;EgB;Cthe_0536) (Cel5A)	3.2.1.4	pubmed	Acetivibrio thermocellus ATCC 27405	ABN51772.1	P04956		1
endo-β-1,4-glucanase G (CelG;Cthe_2872) (Cel5D)	3.2.1.4	pubmed	Acetivibrio thermocellus ATCC 27405	ABN54070.1	Q05332		1
cellobiohydrolase (CelO) (Cel5F)	3.2.1.74	pubmed	Acetivibrio thermocellus F7	CAB76938.1	Q9L3J2		1
endo-β-1,4-glucanase B (CelB;EgB) (Cel5A)	3.2.1.4	pubmed	Acetivibrio thermocellus NCIB 10682	CAA27266.1	P04956		1
endo-β-1,4-glucanase G (CelG) (Cel5D)	3.2.1.4	pubmed	Acetivibrio thermocellus NCIB 10682	CAA49187.1	Q05332		1
endo-β-1,4-glucanase E1 (EGAc; EGI; TcE1; Acel_0614) (Cel5A)	3.2.1.4	pubmed	Acidothermus cellulolyticus 11B	AAA75477.1 ABH84883.1 ABK52387.1 ACW33392.1 CAD42489.1 CAR96693.1	P54583	1C0D[A,B] 1ECE[A,B] 1VRX[A,B]	1
endo-β-1,4-glucanase (Egl;AZC_1301)	3.2.1.4	pubmed	Azorhizobium caulinodans ORS 571	BAF87299.1	A8I0V5		1
endo-β-1,4-glucanase / exo-β-1,4-glucanase (CelB;EgB)	3.2.1.4	pubmed	Caldicellulosiruptor saccharolyticus	CAA31936.1 A43802	P10474		1
cellulase (CBH-B;CelB;Csac_1078)	3.2.1.4	pubmed	Caldicellulosiruptor saccharolyticus DSM 8903	ABP66692.1	A4XIF7		1
multidomain endoglucanase (CelB)	3.2.1.4	pubmed	Caldicellulosiruptor sp. Tok7B.1	AAD30364.1	Q9X3P6		1
endo-β-1,4-glucanase D (CenD;Celf_1924) (Cel5A)	3.2.1.4	pubmed	Cellulomonas fimi ATCC 484	AAA23089.1 AEE46054.1	F4GZL0 P50400		1
endo-β-1,4-glucanase B (CelB;CflB;CelCflB;SP18)	3.2.1.4	pubmed	Cellulomonas flavigena CDBB-531	AAD48494.3 AAD48494.2	Q9S3V3		1
bifunctional cellulase / expansin (CelA;pCM1_0020)	3.2.1.4	pubmed	Clavibacter michiganensis subsp. michiganensis NCPPB 382	CAA44467.2 CAM98473.1	A5CLK3 Q9K5C7		1
cellulase (CelA;pCS0061)	3.2.1.4	doi	Clavibacter sepedonicus ATCC 33113	AAK16222.1 CAQ03244.1	B0RJ54 Q9AF65		1
endo-β-1,4-glucanase 5A (CpCel5A;Cphy_1163) (Cel5A)	3.2.1.4	pubmed	Lachnoclostridium phytofermentans ISDg	ABX41541.1	A9KN42		1
endo-β-1,4-glucanase	3.2.1.4	pubmed	Paenibacillus polymyxa	AAA22631.1 AAY03292.1	P23548		1
endo-β-1,4-glucanase (Endo5A)	3.2.1.4	pubmed	Paenibacillus sp. ICGEB2008 / MTCC 5639	AEB00655.1	G0YA73		1
endo-β-1,4-glucanase (Egl)	3.2.1.4	URL	Paenibacillus sp. KCTC8848P	AAL83749.1	Q8RPZ3		1
CMCase (P66;Ccel_2337)	3.2.1.4	pubmed	Ruminiclostridium cellulolyticum H10	ACL76673.1	B8I5D7		1
cellulase N (CelN; Ccel_0740) (Cel5N)	3.2.1.4	pubmed	Ruminiclostridium cellulolyticum H10	ACL75118.1 AAG45162.1	B8I7W1 Q9EYQ0		1
lichenase (Ra2830;CUS_5609)	3.2.1.73	pubmed	Ruminococcus albus 8	EGC04285.1	E9S8W4		1
endo-β-1,4-glucanase (GH5B;RUM_06330) (Cel5B)	3.2.1.4	pubmed	Ruminococcus champanellensis 18P13 = JCM 17042	CBL16847.1	D4LB52		1
endoglucanase (EGtf2; Cel5B)) (Cel5B)	3.2.1.4	pubmed	Thermobifida fusca TM51	AAP56348.1	Q7X2N2		1
endoglucanase 5B (Tfu_2712; Cel5B) (Cel5B)	3.2.1.4	pubmed	Thermobifida fusca YX	AAZ56745.1	Q47LC7		1
multidomain cellulase	3.2.1.4	doi	thermophilic anaerobe NA10	BAA22939.1	O24820		1
endocellulase (CelA) (Cel5A)	3.2.1.4	doi	Thermus caldophilus GK24	AAK60011.1	Q93Q07		1
endo-β-1,4-glucanase (CelE2, partial)	3.2.1.4	pubmed	uncultured bacterium	ANN44164.1	A0A193DQY0	8FC0[A,B,C,D,E,F,G,H]	1
endo-β-1,4-glucanase (EngXCA;EngA) (frameshift)	3.2.1.4	pubmed	Xanthomonas campestris pv. campestris	CAC18529.1 AAA27612.1	P19487 Q9EVR6	4TUF[A,B,C,D]	1
endo-β-1,4-glucanase (EngXCA;XccEG;XCC3521)	3.2.1.4	doi	Xanthomonas campestris pv. campestris str. ATCC 33913	AAM42791.1 NP_638867.1	P19487		1
endo-β-1,4-glucanase	3.2.1.4	pubmed	Xanthomonas sp. EC102	ACU30064.1	C7FFK1		1
endoglucanase (XF0818)	3.2.1.4	pubmed	Xylella fastidiosa 9a5c	AAF83628.1 NP_298108.1	Q9PF60		1
unclassified
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
β-glucanase	3.2.1.4	pubmed	uncultured organism	ADD61853.1	D9ZEU9		1
cellulase 5K (Cel5K)	3.2.1.4	pubmed	uncultured organism	AEC45566.1	F5CT74		1
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