Glycoside Hydrolase Family 5 / Subf 10

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-)
Activities in Sub Family
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (29); Uniprot accession (14); PDB accession (4); 3D entries (3); cryst (0)
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 Fisuc_0728 / FSU_1166 3.2.1.78 Fibrobacter succinogenes subsp. succinogenes S85 ACX74338.1 C9RMW0   10
 MY04_1929   Flammeovirga sp. MY04 ANQ49303.1     10
 MY04_5409   Flammeovirga sp. MY04 ANQ52741.1     10
 β-1,4-mannanase   Flammeovirga yaeyamensis MY04 ACA05117.2 B1A1I2   10
 LPB138_02855   Lutibacter sp. LPB0138 AOW19684.1     10
 MROS_1693   Melioribacter roseus P3M-2 AFN74927.1     10
 Sde_2285 (ManR) (Gly5S)   Saccharophagus degradans 2-40 ABD81545.1 Q21ID4   10
 STHERM_c00920   Spirochaeta thermophila DSM 6192 ADN01068.1 E0RU12   10
 Spith_0079   Spirochaeta thermophila DSM 6578 AEJ60366.1     10
 TJEJU_1866   Tenacibaculum jejuense KCTC 22618 SNR15572.1     10
 Scaffold00026.9 (Man1)   uncultured bacterium AEV59732.1     10
 BA893_23980   Vibrio natriegens CCUG 16373 ANQ24656.1     10
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