Activities in Family	[retaining] α-1,4-glucan branching enzyme (EC 2.4.1.18); [retaining] 4-α-glucanotransferase / amylomaltase (EC 2.4.1.25); [retaining] α-amylase (EC 3.2.1.1); [retaining] maltogenic α-amylase (EC 3.2.1.133); α-galactosidase (EC 3.2.1.22); [retaining] pullulanase (EC 3.2.1.41); [retaining] cyclomaltodextrinase (EC 3.2.1.54);
Mechanism	Retaining a
3D Structure Status	( β / α ) 7 barrel
Catalytic Nucleophile/Base	Glu
Catalytic Proton Donor	Asp
External resources	CAZypedia;
Statistics	GenBank accession (5285); Uniprot accession (30); PDB accession (12); 3D entries (7); cryst (0)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
glycogen branching enzyme (GBE;PhGBE;PH1386)	2.4.1.18 3.2.1.1	Pyrococcus horikoshii OT3	BAA30492.1 NP_143265.1	O50094	5WU7[A,B] 2.31
maltose-forming α-amylase (PSMA;Py04_0872)	3.2.1.133	Pyrococcus sp. ST04	AFK22464.1	I3RE04	4CMR[A,B] 1.80
bifunctional amylopullulanase cyclomaltodextrinase (Apu-Sm;SMApu;Smar_1407) (intracellular)	3.2.1.1 3.2.1.41 3.2.1.54	Staphylothermus marinus F1	ABN70497.1	A3DPD7	7E1Y[A,B,C,D,E,F,G,H] 2.90
branching enzyme (TkGBE; TK1436)	2.4.1.18	Thermococcus kodakarensis KOD1	BAD85625.1 CAT71088.1	Q5JDJ7	3N8T[A] 2.40 3N92[A] β-D-Glcp 2.89 3N98[A] β-D-Glcp 1.87
4-α-glucanotransferase (Jgt)	2.4.1.25	Thermococcus litoralis	BAA22063.1	O32462	1K1W[A] α-D-Glcp 2.80 1K1X[A,B] 2.40 1K1Y[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp 2.40
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
[retaining] α-1,4-glucan branching enzyme / α-amylase (AmyC; GBE; TM1438)	2.4.1.18 3.2.1.1	Thermotoga maritima MSB8	AHD18669.1 AGL50368.1 AKE29197.1 QFD27285.1	R4P360	2B5D[X] 2.20
glycogen branching enzyme (GBE; TtGBE; TTHA1902; TT1467)	2.4.1.18	Thermus thermophilus HB8	BAD71725.1	Q5SH28	1UFA[A] 2.20 3P0B[A] 1.35
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