Glycoside Hydrolase Family 56

Known Activities	hyaluronidase (EC 3.2.1.35); chondroitin hydrolase (EC 3.2.1.-)
Mechanism	Retaining
3D Structure Status	( β / α ) ₇
Catalytic Nucleophile/Base	carbonyl oxygen of C-2 acetamido group of substrate
Catalytic Proton Donor	Glu
External resources	InterPro; PFAM; PRINTS;
Statistics	GenBank accession (311); Uniprot accession (134); PDB accession (6); 3D entries (3); cryst (0)

Summary

All (159) Eukaryota (158) Viruses (1) Structure (3 ) Characterized (22)

Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
hyaluronidase (HYA;Api m 2;Apim2;LOC406146)	3.2.1.35	Apis mellifera	AAA27730.1 AAB45920.1 AAR78242.1 ABH87698.1 NP_001011619.1	Q08169	1FCQ[A] 1FCU[A] 1FCV[A] 2J88[A]
hyaluronidase (Spam1;PH-20;p80)	3.2.1.35	Bos taurus	AAI10184.1 AAP55713.1 AAV41022.1	Q2YDK3 Q7YS45
hyaluronidase / chondroitinase (Hyα-1;T22C8.2)	3.2.1.- 3.2.1.35	Caenorhabditis elegans Bristol N2	BAG48551.1 CAA88874.1 NP_495830.1	Q22675
hyaluronidase PH-20	3.2.1.35	Cavia porcellus	AAB45922.1 CAA39768.1	P23613.1
hyaluronidase (Dol m 2)	3.2.1.35	Dolichovespula maculata	AAA68279.1 AAR78243.1 ABH87699.1 CAJ98548.1	P49371
hyaluronidase (PH-20;SPAM1;HYAL3;PH20) (sperm)	3.2.1.35	Homo sapiens	AAC16482.1 AAC60607.2 AAH26163.1 AAQ96882.1 AAX13985.1 AAX13986.1 AAX13987.1 AAX13988.1 AAY03322.1 ABJ38775.1 ACM84481.1 BAF84918.1 CAA59086.1 NP_003108.2 NP_694859.1	P38567 Q8TC30
chondroitin sulfate hydrolase / hyaluronidase 3 (HYAL3;LUCA-3;LUCA14.2)	3.2.1.- 3.2.1.35	Homo sapiens	AAC02729.1 AAC70915.1 AAD04257.1 AAH05896.1 AAH12892.1 AAM60775.1 AAM60776.1 AAM60777.1 AAM60778.1 ABA67787.1 BAG36242.1 NP_003540.2	O43820 O60540 Q8NFK2 Q8NFK3 Q8NFK4 Q96E56 Q9BRW9
chondroitin sulfate hydrolase / hyaluronidase 1 (HYAL1;Hyal-1;LUCA1;LUCA-1)	3.2.1.- 3.2.1.35	Homo sapiens	AAB67046.1 AAC02730.1 AAD04190.1 AAD09137.2 AAD53277.1 AAE60457.1 AAE60459.1 AAH25774.1 AAH35695.1 AAM60770.1 AAM60771.1 AAM60772.1 AAM60773.1 AAM60774.1 ABJ38770.1 ABJ38772.1 ACK03148.1 BAF83505.1 BAG53447.1 CAC60225.1 CAG46731.1 NP_009296.1 NP_149349.2 NP_695013.1 NP_695014.1 NP_695015.1 NP_695016.1 NP_695017.1 NP_695018.1	Q12794.2 Q6FH23	2PE4[A]
chondroitin sulfate hydrolase / hyaluronidase 2 (Hyal2;LUCA2;LUCA-2)	3.2.1.- 3.2.1.35	Homo sapiens	AAB67045.1 AAC28656.1 AAC62823.1 AAH00692.1 ABA82488.1 ACK03149.1 BAG52554.1 BAG54602.1 CAA03924.1 NP_003764.3 NP_149348.2	B3KRZ2 Q12891.3
chondroitin sulfate hydrolase / hyaluronoglucosaminidase 4 / hyaluronidase 4 (Hyal4)	3.2.1.-	Homo sapiens	AAC98883.1 AAD43186.1 AAI04789.1 AAI04791.1 ACM81328.1 ACM82678.1 ACM82678.1 ACV97636.1 BAI49593.1 NP_036401.1	D0VXG1 Q2M3T9 Q9UL99
hyaluronidase	3.2.1.35	Macaca fascicularis	ABD94494.1 BAE01371.1	P38568
venom hyaluronidase (BmHYA1) (fragment)	3.2.1.35	Mesobuthus martensii	ACY69673.1	D1MBU1
hyaluronidase (Spam1;Hyal1;PH-20;Spam)	3.2.1.35	Mus musculus	AAA76603.1 AAP49832.1 AAX13989.1 AAX13990.1 BAB24161.1 BAC55070.1 NP_033267.1	P48794 P48794.2 Q5D1J0
hyaluronoglucosaminidase 1	3.2.1.35	Mus musculus	AAC15949.1 AAH21636.1 AAL17822.1 BAC26206.1 CAP19319.1 CAP19320.1 CAP19321.1 CAP19322.1 NP_032343.2	O70229 Q8CE62 Q8VDK0 Q91ZJ9
hyaluronidase (Hyal1;Spam1;PH20)	3.2.1.35	Oryctolagus cuniculus	AAA88913.1	P38566
hyaluronidase (Pol a 2)	3.2.1.35	Polistes annularis	AAD52616.1 AAR78239.1 ABH87695.1	Q9U6V9
hyaluronidase 2 (Hyal2)	3.2.1.35	Rattus norvegicus	AAD01980.1 AAH62410.1 AAO49504.1 NP_742037.1	Q80ZC7 Q9Z2Q3
hyaluronidase (HYA1;SfHya1)	3.2.1.35	Synanceia horrida	AAO74499.1	Q801Z8
hyaluronidase (Ves v 2 allergen)	3.2.1.35	Vespula vulgaris	AAB48073.1 AAR78244.1 ABH87700.1	P49370	2ATM[A]
hyaluronidase XKH1	3.2.1.35	Xenopus laevis	AAI69767.1 AAI69795.1 AAL67683.1	Q8UVT7
lysosomal hyaluronidase (Hyal2)	3.2.1.35	Xenopus laevis	AAL55823.1	Q8UVY6
hyaluronidase (embryonic) XEH1	3.2.1.35	Xenopus laevis	AAD28281.1	Q9W6K5

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.