Activities in Family	Endo-β-1,4-galactanase (EC 3.2.1.89);
Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
External resources	CAZypedia;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (5500); Uniprot accession (25); PDB accession (16); 3D entries (7); cryst (0)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
endo-β-1,4-galactanase (IaGal;Igag_0688)	3.2.1.89	Ignisphaera aggregans DSM 17230	ADM27518.1	E0SSW8	7OSK[A,B] 2.65
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
endo-β-1,4-galactanase (BLGAL; YvfO; BL00263; BLi04276)	3.2.1.89	Bacillus licheniformis DSM 13 = ATCC 14580	AAU25711.1 AAO31370.1 AAU43089.1 ACS08442.1	Q62NF0 Q65CX5	1R8L[A,B] 2.60 1UR0[A,B] β-D-Galp-(1-4)-β-D-Galp-(1-4)-β-D-Galp 2.5 1UR4[A,B] β-D-Galp-(1-4)-β-D-Galp 2.2 2CCR[A,B] β-D-Galp-(1-4)-β-D-Galp β-D-Galp-(1-4)-β-D-Galp-(1-4)-β-D-Galp 2.3 2GFT[A,B] β-D-Galp-(1-4)-β-D-Galp-(1-4)-β-D-Galp 2.30 2J74[A,B] β-D-Galp-(1-4)-β-D-Galp β-D-Galp-(1-4)-β-D-Galp-(1-4)-β-D-Galp 2.6
endo-β-1,4-galactanase (BtGH53;BT4668;BT_4668)	3.2.1.89	Bacteroides thetaiotaomicron VPI-5482	AAO79773.1 NP_813579.1	Q89YR3	6GP5[A,B] 1.93 6GPA[A,B] β-D-Galp 1.79
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
endo-β-1,4-galactanase 1 (Gal1; AAGAL)	3.2.1.89	Aspergillus aculeatus KSM 510	AAA32692.1	P48842	1FHL[A] 2.30 1FOB[A] 1.80 6Q3R[A,B] β-D-Galp-(1-4)-β-D-Galp 2.69
endo-β-1,4-galactanase (EnGAL;AN5727.2)	3.2.1.89	Aspergillus nidulans FGSC A4	ABF50874.1 EAA62820.1	Q5B153	4BF7[A] 2.00
endo-β-1,4-galactanase (HiGal)	3.2.1.89	Humicola insolens	AAE73521.1 AAN99815.1 CAG44583.1	P83691	1HJQ[A] 2.55
endo-β-1,4-galactanase (MtGal)	3.2.1.89	Thermothelomyces thermophilus	AAE73520.1 AAN99814.1 ACS08439.1	P83692	1HJS[A,B,C,D] 1.87 1HJU[A,B,C,D] 2.15
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