Glycoside Hydrolase Family 44

Known Activities	endoglucanase (EC 3.2.1.4); xyloglucanase (EC 3.2.1.151)
Mechanism	Retaining
3D Structure Status	( β / α ) ₈
Catalytic Nucleophile/Base	Glu
Catalytic Proton Donor	Glu
Note	formerly known as cellulase family J. Note that the stereochemistry of the reaction has recently been corrected : Kitago et al., J. Biol. Chem. 282(2007)35703-11 (PMID: 17905739)
External resources	CAZypedia;
Commercial Enzyme Provider(s)	NZYTech; PROZOMIX;
Statistics	GenBank accession (70); Uniprot accession (39); PDB accession (14); 3D entries (4); cryst (0)

Summary

All (58) Bacteria (55) Eukaryota (3) Structure (4 ) Characterized (11)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
bifunctional β-mannanase / endo-β-1,4-glucanase (ManA)	3.2.1.78	Caldicellulosiruptor saccharolyticus	AAA71887.1	P22533.2
endoglucanase (EG;CAC0915)	3.2.1.4 3.2.1.8	Clostridium acetobutylicum ATCC 824	AAK78891.1 NP_347551.1	Q977Y3	3IK2[A]
endoglucanase (CelJ) (Cel9D-Cel44A)	3.2.1.4	Clostridium thermocellum F1	BAA12070.1	P71140	2D8G 2E0P[A] 2E4T[A] 2EEX[A] 2EJ1[A] 2EO7[A] 2EQD[A]
endo-β-1,4-glucanase A (CelA)	3.2.1.4	Paenibacillus lautus PL236	AAA22303.1 CAA01933.1	P29719.1
xyloglucanase	3.2.1.151	Paenibacillus pabuli	AAW12878.1 ACE10761.1
xyloglucanase	3.2.1.151	Paenibacillus polymyxa	AAW12877.1 ACE10760.1
xyloglucanase / β-mannanase (Cel44C-Man26A)	3.2.1.4 3.2.1.78	Paenibacillus polymyxa GS01	AAW12876.1 ABC88431.1 ACE10759.1		2YIH[A] 2YJQ[A,B] 2YKK[A] 3ZQ9[A]
cellulase (EndB)	3.2.1.4	Ruminococcus flavefaciens 17	CAC83072.1	Q934F9
xyloglucanase / endo-β-1,4-glucanase B (CelB)	3.2.1.151 3.2.1.4	Ruminococcus flavefaciens FD-1	AAA95959.1	Q52743
bifunctional glucanase-xylanase (CelM2)	3.2.1.4 3.2.1.8	uncultured bacterium	ABL11223.1	A1E9A6	3FW6[A] 3II1[A]
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
endoglucanase (37GP1)	3.2.1.4	Bankia gouldi	AAM56790.1 ABW61101.1 ABY00380.1 CAH68691.1 CAT81451.1

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.