Glycoside Hydrolase Family 43

Activities in Familyβ-xylosidase (EC 3.2.1.37); α-L-arabinofuranosidase (EC 3.2.1.55); arabinanase (EC 3.2.1.99); xylanase (EC 3.2.1.8); galactan 1,3-β-galactosidase (EC 3.2.1.145); α-1,2-L-arabinofuranosidase (EC 3.2.1.-); exo-α-1,5-L-arabinofuranosidase (EC 3.2.1.-); [inverting] exo-α-1,5-L-arabinanase (EC 3.2.1.-); β-1,3-xylosidase (EC 3.2.1.-)
Mechanism Inverting
ClanGH-F
3D Structure Status5-fold β-propeller
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorGlu
NoteMany members have been assigned to subfamilies as described by Mewis et al. (2016) Appl. Environm. Microbiol. in press (PMID: 26729713).
External resourcesCAZypedia;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (9168); Uniprot accession (1416); PDB accession (81); 3D entries (39); cryst (3)
All (8596) Archaea (17) Bacteria (8175) Eukaryota (372) Viruses (5) unclassified (27) Structure (39 - 3 cryst) Characterized (151)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 β-xylosidase (XynB;XylBH43;BH3683) 3.2.1.37 Bacillus halodurans C-125 BAB07402.1
NP_244550.1
Q9K6P5
1YRZ[A,B] 2.00
11
 BLi04220 / BL00219 (YxiA)   Bacillus licheniformis DSM 13 = ATCC 14580 AAU43033.1
AAU25654.1
AAU25654.2
ACR87831.1
CAF05510.1
CAV28398.1
Q65D31
3LV4[A,B] 1.70
4
 endo-α-1,5-L-arabinanase (Abn2;YxiA;J3A;BSU39330) (Arb43B) 3.2.1.99 Bacillus subtilis subsp. subtilis str. 168 CAB15969.2
ACB06752.1
AIY95249.1
AQR83975.1
AQR88190.1
CAB15969.1
NP_391812.1
B3FRL6
P42293
2X8F[A,B] 1.90
2X8S[A,B] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf
1.50
2X8T[A,B] 1.79
4COT[A] 1.90
4
 endo-α-1,5-L-arabinanase (AbnA;BSU28810) (Arb43A) 3.2.1.99 Bacillus subtilis subsp. subtilis str. 168 CAA99586.1
AAV87172.1
AIY94210.1
AQR82908.1
AQR87123.1
CAB14841.1
CAB14841.2
NP_390759.1
P94522
1UV4[A] 1.50
5
 β-1,4-xylosidase (BsX;XynB;YnaK;BSU17580) 3.2.1.37 Bacillus subtilis subsp. subtilis str. 168 AAB41091.1
AIY93062.1
AQR81741.1
AQR85955.1
CAB13642.1
CAB13642.2
NP_389640.1
P94489
1YIF[A,B,C,D] 1.80
11
 arabinoxylan α-L-1,3-arabinofuranohydrolase (XynD;BsAXH-m23;BSU18160) 3.2.1.55 Bacillus subtilis subsp. subtilis str. 168 CAB13699.1
AIY93126.1
AQR81807.1
AQR86021.1
NP_389698.1
Q45071
3C7E[A] 2.00
3C7F[A] β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
1.55
3C7G[A] β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
2.02
3C7H[A] β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
2.00
3C7O[A] β-D-Glcp-(1-4)-β-D-Galp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.80
16
 α-L-arabinofuranoside (Xynb_4;BoGH43A;BACOVA_02654;Bovatus_03073) 3.2.1.55 Bacteroides ovatus ATCC 8483 ALJ47681.1  
5JOW[A,B] 1.60
5JOX[A,B] <carb_like_EDG>
1.80
5JOY[A,B] α-L-Araf1N-(1-1)-<non_carb>
β-L-Araf1N-(1-1)-<non_carb>
1.90
12
 α-L-arabinofuranoside (Xynb_5;BoGH43B;BACOVA_02656;Bovatus_03074) 3.2.1.55 Bacteroides ovatus ATCC 8483 ALJ47682.1  
5JOZ[A,B] 2.28
12
 α-L-arabinofuranosidase (BT3675;BT_3675) 3.2.1.55 Bacteroides thetaiotaomicron VPI-5482 AAO78780.1
NP_812586.1
Q8A1I6
3QZ4[A,B] 1.74
34
 BT2959 / BT_2959   Bacteroides thetaiotaomicron VPI-5482 AAO78065.1
NP_811871.1
Q8A3J5
3NQH[A] 2.11
24
 endo-arabinase (BT1873)   Bacteroides thetaiotaomicron VPI-5482 AAO76980.1
NP_810786.1
Q8A6K8
5C0P[A] 1.53
17
 BT2895   Bacteroides thetaiotaomicron VPI-5482 AAO78001.1
NP_811807.1
Q8A3Q9
3KST[A,B] 1.70
31
 (grape RGII active) α-L-arabinofuranosidase (BT3662;BT_3662) 3.2.1.55 Bacteroides thetaiotaomicron VPI-5482 AAO78767.1
NP_812573.1
Q8A1J9
5MSX[A,B,C] 2.10
34
 exo-β-1,3-galactanase (BBIF_0009)   Bifidobacterium bifidum S17 ADO52214.1 E3END6
5FLW[A,B] 1.40
24
 α-L-arabinofuranosidase (Xsa43E;bpr_I2319) 3.2.1.37
3.2.1.55
Butyrivibrio proteoclasticus B316 ADL35052.1 E0RYY0
4NOV[A] 1.33
29
 endo-α-1,5-L-arabinanase / exo-α-1,5-L-arabinanase 43A (ArbA;CJA_0805) (Arb43A) 3.2.1.99
3.2.1.-
Cellvibrio japonicus Ueda107 ACE84667.1
CAA71485.1
B3PKP8
P95470
1GYD[B] 2.05
1GYE[B] α-L-Araf-(1-5)-α-L-Araf
2.5
1GYH[A,B,C,D,E,F] 1.89
5
 α-1,2-L-arabinofuranosidase 43A (CJA_3018) (arabinan-specific) (Abf43A=Gly43N) 3.2.1.- Cellvibrio japonicus Ueda107 ACE83886.1 B3PD60
3QED[A,B,C,D] 2.99
3QEE[A,B] 1.64
3QEF[A,B] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf
α-L-Araf-(1-5)[α-L-Araf-(1-3)]-α-L-Araf-(1-5)-α-L-Araf
1.79
29
 β-xylosidase (XynD;CAC3452)   Clostridium acetobutylicum ATCC 824 AAK81382.1
NP_350042.1
Q97DM1
1Y7B[A,B,C,D] 1.60
1YI7[A,B,C,D] 1.90
11
 β-xylosidase (Abf2;CAC1529)   Clostridium acetobutylicum ATCC 824 AAK79496.1
NP_348156.1
Q97IW1
3CPN[A] 1.55
3K1U[A] 1.55
26
 β-xylosidase (XynB3;HUS_CDS105) 3.2.1.37 Geobacillus stearothermophilus T-6 NCIMB 40222 AAT98625.1
ABI49959.1
Q09LX0
2EXH[A,B,C,D] 1.88
2EXI[A,B,C,D] 2.15
2EXJ[A,B,C,D] β-D-Xylp-(1-4)-β-D-Xylp
2.20
2EXK[A,B,C,D] β-D-Xylp-(1-4)-β-D-Xylp
2.20
11
 α-1,5-L-arabinanase (AbnB;HUS_CDS25) (intracellular) 3.2.1.99 Geobacillus stearothermophilus T-6 NCIMB 40222 ACE73676.1 B3EYM8
3CU9[A] 1.06
3D5Y[A] 1.22
3D5Z[A] α-L-Araf-(1-5)-α-L-Araf-(1-5)-β-L-Araf
1.90
3D60[A] 1.90
3D61[A] α-L-Araf-(1-5)-β-L-Araf
1.95
5
 endo-α-1,5-L-arabinanase (AbnA) (extracellular) 3.2.1.99 Geobacillus stearothermophilus T-6 NCIMB 40222 ACE73680.1 B3EYN2
5HO0[A] 2.35
5HO2[A] 2.37
5HO9[A,B] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf
2.85
5HOF[A] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf
2.96
5HP6[A] 2.09
4
 endo-α-1,5-L-arabinanase (Abn-ts) 3.2.1.99 Geobacillus thermodenitrificans TS-3 BAB64339.1 Q93HT9
1WL7[A] 1.90
5
 β-xylosidase (Xyl;GbtXyl43A) (Xyl43A) 3.2.1.37 Geobacillus thermoleovorans IT-08 ABC75004.1 Q2I2N4
cryst    
12
 α-L-arabinofuranosidase (HoAraf43;Hore_20580) 3.2.1.55 Halothermothrix orenii H 168 ACL70803.1 B8CZV1
4QQS[A,B] 1.10
33
 α-L-arabinofuranosidase II (Araf43;LbAraf43;N624_1993) 3.2.1.- Lactobacillus brevis DSM 1269 / TMW 1.313 APU52333.1
KIO95879.1
 
5M8B[A,B] 1.90
26
 [arabinogalactan] exo-β-1,3-galactanase (Ct1,3Gal43A;CtGH43;Cthe_0661) (1,3Gal43A) 3.2.1.145 Ruminiclostridium thermocellum ATCC 27405 ABN51896.1 A3DD67
3VSF[A,B,C,D,E,F] 2.76
3VSZ[A,B,C,D,E,F] β-D-Galp-(1-3)-β-D-Galp
β-D-Galp-(1-3)-β-D-Galp-(1-3)-β-D-Galp
2.89
3VT0[A,B,C,D,E,F] β-D-Galp-(1-4)-β-D-Glcp
2.91
3VT1[A,B,C,D,E,F] β-D-Galp
3.19
3VT2[A,B,C,D,E,F] β-D-Galp1S-(1-1)-<non_carb>
3.00
24
 α-L-arabinofuranosidase (Ct43Araf;CtAbf43A;CtGh43;Cthe_1271) (Abf43A) 3.2.1.55 Ruminiclostridium thermocellum ATCC 27405 ABN52503.1 A3DEX4
5A8C[A] 0.97
5A8D[A] 1.65
16
 β-xylosidase / α-L-arabinofuranosidase (Xsa;Sxa;SXA) 3.2.1.37
3.2.1.55
Selenomonas ruminantium GA192 AAB97967.1 O52575
3C2U[A,B,C,D] 1.30
11
 exo-α-1,5-L-arabinofuranosidase (SaAraf43A;SAV1043) (Araf43A) 3.2.1.- Streptomyces avermitilis MA-4680 = NBRC 14893 BAC68753.1
NP_822218.1
 
3AKF[A] 2.20
3AKG[A] α-L-Araf
α-L-Araf-(1-5)-α-L-Araf
1.80
3AKH[A] α-L-Araf
α-L-Araf-(1-5)-α-L-Araf
1.70
3AKI[A] α-L-Araf1N-(1-1)-<non_carb>
2.00
26
 endo-α-1,5-L-arabinanase (AbnA;TpABN;Tpet_0637) 3.2.1.99 Thermotoga petrophila RKU-1 ABQ46657.1 A5IKD4
4KC7[A,B,C] 1.75
4KC8[A,B,C] 1.76
4
 β-xylosidase / α-L-arabinofuranosidase (CoXyl43) 3.2.1.37
3.2.1.55
uncultured bacterium BAS02080.1  
5GLK[A,B] 1.70
5GLL[A,B] 1.80
5GLM[A,B] β-D-Xylp-(1-4)-β-D-Xylp
β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
1.80
5GLN[A,B] α-D-Xylp
β-D-Xylp
β-D-Xylp-(1-4)-β-D-Xylp
β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
1.70
5GLO[A,B] α-L-Arap
β-L-Araf
1.80
5GLP[A,B] α-L-Arap
1.80
5GLQ[A,B] β-D-Xylp-(1-4)-β-D-Xylp
β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
β-L-Araf
1.70
5GLR[A,B] β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
β-L-Araf
1.70
1
 exo-α-1,5-L-arabinanase (Arn3;ARN3) 3.2.1.- uncultured bacterium ADB43999.1 D2XML8
4KCB[A,B] 2.90
5
 endo-α-1,5-L-arabinanase (Arn2;ARN2) (partial) 3.2.1.99 uncultured rumen bacterium    
4KCA[A] 1.90
4KCA[B] 1.90
4
 α-L-arabinofuranosidase (Araf43;WAraf43) 3.2.1.- Weissella sp. 142 APU52332.1  
5M8E[A,B] 2.00
26
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 double substituted xylan α-1,3-L-arabinofuranosidase (α-araF;AXHd3;;Abf II) 3.2.1.55 Humicola insolens DSM 18000 CAL81199.1  
3ZXJ[A,B] 1.85
3ZXK[A,B] β-D-Xylp-(1-4)[α-L-Araf-(1-2)]-β-D-Xylp-(1-4)-β-D-Xylp
1.44
3ZXL[A,B] 1.87
36
 endo-α-1,5-L-arabinanase (AbnS1) 3.2.1.99 Penicillium chrysogenum 31B BAD89094.2
BAD89094.1
BAJ49868.1
Q5H7M8
cryst    
6
 exo-β-1,3-galactanase (Pc1,3Gal43A) (1,3Gal43A) 3.2.1.145 Phanerochaete chrysosporium BAD98241.1 Q50KB2
cryst    
24
unclassified
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 β-xylosidase (RS223-BX) 3.2.1.37 uncultured organism AFP23142.1  
4MLG[A,B,C,D,E,F,G,H,I,J, 2.70
1
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