Activities in Family	β-xylosidase (EC 3.2.1.37); α-L-arabinofuranosidase (EC 3.2.1.55); arabinanase (EC 3.2.1.99); xylanase (EC 3.2.1.8); galactan 1,3-β-galactosidase (EC 3.2.1.145); α-1,2-L-arabinofuranosidase (EC 3.2.1.-); exo-α-1,5-L-arabinofuranosidase (EC 3.2.1.-); [inverting] exo-α-1,5-L-arabinanase (EC 3.2.1.-); β-1,3-xylosidase (EC 3.2.1.-)
Mechanism	Inverting
Clan	GH-F
3D Structure Status	5-fold β-propeller
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Glu
Note	Many members have been assigned to subfamilies as described by Mewis et al. (2016) Appl. Environm. Microbiol. in press (PMID: 26729713).
External resources	CAZypedia;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (8615); Uniprot accession (1416); PDB accession (81); 3D entries (39); cryst (3)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
β-xylosidase (XynB;XylBH43;BH3683)	3.2.1.37	Bacillus halodurans C-125	BAB07402.1 NP_244550.1	Q9K6P5	1YRZ[A,B] 2.00	11
BLi04220 / BL00219 (YxiA)		Bacillus licheniformis DSM 13 = ATCC 14580	AAU43033.1 AAU25654.1 AAU25654.2 ACR87831.1 CAF05510.1 CAV28398.1	Q65D31	3LV4[A,B] 1.70	4
endo-α-1,5-L-arabinanase (Abn2;YxiA;J3A;BSU39330) (Arb43B)	3.2.1.99	Bacillus subtilis subsp. subtilis str. 168	CAB15969.2 ACB06752.1 AIY95249.1 AQR83975.1 AQR88190.1 CAB15969.1 NP_391812.1	B3FRL6 P42293	2X8F[A,B] 1.90 2X8S[A,B] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf 1.50 2X8T[A,B] 1.79 4COT[A] 1.90	4
endo-α-1,5-L-arabinanase (AbnA;BSU28810) (Arb43A)	3.2.1.99	Bacillus subtilis subsp. subtilis str. 168	CAA99586.1 AAV87172.1 AIY94210.1 AQR82908.1 AQR87123.1 CAB14841.1 CAB14841.2 NP_390759.1	P94522	1UV4[A] 1.50	5
β-1,4-xylosidase (BsX;XynB;YnaK;BSU17580)	3.2.1.37	Bacillus subtilis subsp. subtilis str. 168	AAB41091.1 AIY93062.1 AQR81741.1 AQR85955.1 CAB13642.1 CAB13642.2 NP_389640.1	P94489	1YIF[A,B,C,D] 1.80	11
arabinoxylan α-L-1,3-arabinofuranohydrolase (XynD;BsAXH-m23;BSU18160)	3.2.1.55	Bacillus subtilis subsp. subtilis str. 168	CAB13699.1 AIY93126.1 AQR81807.1 AQR86021.1 NP_389698.1	Q45071	3C7E[A] 2.00 3C7F[A] β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp 1.55 3C7G[A] β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp 2.02 3C7H[A] β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp 2.00 3C7O[A] β-D-Glcp-(1-4)-β-D-Galp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.80	16
α-L-arabinofuranoside (Xynb_4;BoGH43A;BACOVA_02654;Bovatus_03073)	3.2.1.55	Bacteroides ovatus ATCC 8483	ALJ47681.1		5JOW[A,B] 1.60 5JOX[A,B] <carb_like_EDG> 1.80 5JOY[A,B] α-L-Araf1N-(1-1)-<non_carb> β-L-Araf1N-(1-1)-<non_carb> 1.90	12
α-L-arabinofuranoside (Xynb_5;BoGH43B;BACOVA_02656;Bovatus_03074)	3.2.1.55	Bacteroides ovatus ATCC 8483	ALJ47682.1		5JOZ[A,B] 2.28	12
α-L-arabinofuranosidase (BT3675;BT_3675)	3.2.1.55	Bacteroides thetaiotaomicron VPI-5482	AAO78780.1 NP_812586.1	Q8A1I6	3QZ4[A,B] 1.74	34
BT2959 / BT_2959		Bacteroides thetaiotaomicron VPI-5482	AAO78065.1 NP_811871.1	Q8A3J5	3NQH[A] 2.11	24
endo-arabinase (BT1873)		Bacteroides thetaiotaomicron VPI-5482	AAO76980.1 NP_810786.1	Q8A6K8	5C0P[A] 1.53	17
BT2895		Bacteroides thetaiotaomicron VPI-5482	AAO78001.1 NP_811807.1	Q8A3Q9	3KST[A,B] 1.70	31
(grape RGII active) α-L-arabinofuranosidase (BT3662;BT_3662)	3.2.1.55	Bacteroides thetaiotaomicron VPI-5482	AAO78767.1 NP_812573.1	Q8A1J9	5MSX[A,B,C] 2.10	34
exo-β-1,3-galactanase (BBIF_0009)		Bifidobacterium bifidum S17	ADO52214.1	E3END6	5FLW[A,B] 1.40	24
α-L-arabinofuranosidase (Xsa43E;bpr_I2319)	3.2.1.37 3.2.1.55	Butyrivibrio proteoclasticus B316	ADL35052.1	E0RYY0	4NOV[A] 1.33	29
endo-α-1,5-L-arabinanase / exo-α-1,5-L-arabinanase 43A (ArbA;CJA_0805) (Arb43A)	3.2.1.99 3.2.1.-	Cellvibrio japonicus Ueda107	ACE84667.1 CAA71485.1	B3PKP8 P95470	1GYD[B] 2.05 1GYE[B] α-L-Araf-(1-5)-α-L-Araf 2.5 1GYH[A,B,C,D,E,F] 1.89	5
α-1,2-L-arabinofuranosidase 43A (CJA_3018) (arabinan-specific) (Abf43A=Gly43N)	3.2.1.-	Cellvibrio japonicus Ueda107	ACE83886.1	B3PD60	3QED[A,B,C,D] 2.99 3QEE[A,B] 1.64 3QEF[A,B] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf α-L-Araf-(1-5)[α-L-Araf-(1-3)]-α-L-Araf-(1-5)-α-L-Araf 1.79	29
β-xylosidase (XynD;CAC3452)		Clostridium acetobutylicum ATCC 824	AAK81382.1 NP_350042.1	Q97DM1	1Y7B[A,B,C,D] 1.60 1YI7[A,B,C,D] 1.90	11
β-xylosidase (Abf2;CAC1529)		Clostridium acetobutylicum ATCC 824	AAK79496.1 NP_348156.1	Q97IW1	3CPN[A] 1.55 3K1U[A] 1.55	26
β-xylosidase (XynB3;HUS_CDS105)	3.2.1.37	Geobacillus stearothermophilus T-6 NCIMB 40222	AAT98625.1 ABI49959.1	Q09LX0	2EXH[A,B,C,D] 1.88 2EXI[A,B,C,D] 2.15 2EXJ[A,B,C,D] β-D-Xylp-(1-4)-β-D-Xylp 2.20 2EXK[A,B,C,D] β-D-Xylp-(1-4)-β-D-Xylp 2.20	11
α-1,5-L-arabinanase (AbnB;HUS_CDS25) (intracellular)	3.2.1.99	Geobacillus stearothermophilus T-6 NCIMB 40222	ACE73676.1	B3EYM8	3CU9[A] 1.06 3D5Y[A] 1.22 3D5Z[A] α-L-Araf-(1-5)-α-L-Araf-(1-5)-β-L-Araf 1.90 3D60[A] 1.90 3D61[A] α-L-Araf-(1-5)-β-L-Araf 1.95	5
endo-α-1,5-L-arabinanase (AbnA) (extracellular)	3.2.1.99	Geobacillus stearothermophilus T-6 NCIMB 40222	ACE73680.1	B3EYN2	5HO0[A] 2.35 5HO2[A] 2.37 5HO9[A,B] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf 2.85 5HOF[A] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf 2.96 5HP6[A] 2.09	4
endo-α-1,5-L-arabinanase (Abn-ts)	3.2.1.99	Geobacillus thermodenitrificans TS-3	BAB64339.1	Q93HT9	1WL7[A] 1.90	5
β-xylosidase (Xyl;GbtXyl43A) (Xyl43A)	3.2.1.37	Geobacillus thermoleovorans IT-08	ABC75004.1	Q2I2N4	cryst	12
α-L-arabinofuranosidase (HoAraf43;Hore_20580)	3.2.1.55	Halothermothrix orenii H 168	ACL70803.1	B8CZV1	4QQS[A,B] 1.10	33
α-L-arabinofuranosidase II (Araf43;LbAraf43;N624_1993)	3.2.1.-	Lactobacillus brevis DSM 1269 / TMW 1.313	APU52333.1 KIO95879.1		5M8B[A,B] 1.90	26
[arabinogalactan] exo-β-1,3-galactanase (Ct1,3Gal43A;CtGH43;Cthe_0661) (1,3Gal43A)	3.2.1.145	Ruminiclostridium thermocellum ATCC 27405	ABN51896.1	A3DD67	3VSF[A,B,C,D,E,F] 2.76 3VSZ[A,B,C,D,E,F] β-D-Galp-(1-3)-β-D-Galp β-D-Galp-(1-3)-β-D-Galp-(1-3)-β-D-Galp 2.89 3VT0[A,B,C,D,E,F] β-D-Galp-(1-4)-β-D-Glcp 2.91 3VT1[A,B,C,D,E,F] β-D-Galp 3.19 3VT2[A,B,C,D,E,F] β-D-Galp1S-(1-1)-<non_carb> 3.00	24
α-L-arabinofuranosidase (Ct43Araf;CtAbf43A;CtGh43;Cthe_1271) (Abf43A)	3.2.1.55	Ruminiclostridium thermocellum ATCC 27405	ABN52503.1	A3DEX4	5A8C[A] 0.97 5A8D[A] 1.65	16
β-xylosidase / α-L-arabinofuranosidase (Xsa;Sxa;SXA)	3.2.1.37 3.2.1.55	Selenomonas ruminantium GA192	AAB97967.1	O52575	3C2U[A,B,C,D] 1.30	11
exo-α-1,5-L-arabinofuranosidase (SaAraf43A;SAV1043) (Araf43A)	3.2.1.-	Streptomyces avermitilis MA-4680 = NBRC 14893	BAC68753.1 NP_822218.1		3AKF[A] 2.20 3AKG[A] α-L-Araf α-L-Araf-(1-5)-α-L-Araf 1.80 3AKH[A] α-L-Araf α-L-Araf-(1-5)-α-L-Araf 1.70 3AKI[A] α-L-Araf1N-(1-1)-<non_carb> 2.00	26
endo-α-1,5-L-arabinanase (AbnA;TpABN;Tpet_0637)	3.2.1.99	Thermotoga petrophila RKU-1	ABQ46657.1	A5IKD4	4KC7[A,B,C] 1.75 4KC8[A,B,C] 1.76	4
β-xylosidase / α-L-arabinofuranosidase (CoXyl43)	3.2.1.37 3.2.1.55	uncultured bacterium	BAS02080.1		5GLK[A,B] 1.70 5GLL[A,B] 1.80 5GLM[A,B] β-D-Xylp-(1-4)-β-D-Xylp β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp 1.80 5GLN[A,B] α-D-Xylp β-D-Xylp β-D-Xylp-(1-4)-β-D-Xylp β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp 1.70 5GLO[A,B] α-L-Arap β-L-Araf 1.80 5GLP[A,B] α-L-Arap 1.80 5GLQ[A,B] β-D-Xylp-(1-4)-β-D-Xylp β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp β-L-Araf 1.70 5GLR[A,B] β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp β-L-Araf 1.70	1
exo-α-1,5-L-arabinanase (Arn3;ARN3)	3.2.1.-	uncultured bacterium	ADB43999.1	D2XML8	4KCB[A,B] 2.90	5
endo-α-1,5-L-arabinanase (Arn2;ARN2) (partial)	3.2.1.99	uncultured rumen bacterium			4KCA[A] 1.90 4KCA[B] 1.90	4
α-L-arabinofuranosidase (Araf43;WAraf43)	3.2.1.-	Weissella sp. 142	APU52332.1		5M8E[A,B] 2.00	26
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
double substituted xylan α-1,3-L-arabinofuranosidase (α-araF;AXHd3;;Abf II)	3.2.1.55	Humicola insolens DSM 18000	CAL81199.1		3ZXJ[A,B] 1.85 3ZXK[A,B] β-D-Xylp-(1-4)[α-L-Araf-(1-2)]-β-D-Xylp-(1-4)-β-D-Xylp 1.44 3ZXL[A,B] 1.87	36
endo-α-1,5-L-arabinanase (AbnS1)	3.2.1.99	Penicillium chrysogenum 31B	BAD89094.2 BAD89094.1 BAJ49868.1	Q5H7M8	cryst	6
exo-β-1,3-galactanase (Pc1,3Gal43A) (1,3Gal43A)	3.2.1.145	Phanerochaete chrysosporium	BAD98241.1	Q50KB2	cryst	24
unclassified
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
β-xylosidase (RS223-BX)	3.2.1.37	uncultured organism	AFP23142.1		4MLG[A,B,C,D,E,F,G,H,I,J, 2.70	1
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