Glycoside Hydrolase Family 43 / Subf 5

Activities in Sub FamilyExo-α-1,5-L-arabinofuranosidase (EC 3.2.1.-);
[inverting] endo-α-1,5-L-arabinanase (EC 3.2.1.99);
Mechanism Inverting e
ClanGH-F
3D Structure Status5-fold β-propeller
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorGlu
NoteMany members have been assigned to subfamilies as described by Mewis et al. (2016) Appl. Environm. Microbiol. 82:1686-1692 (PMID: 26729713).
External resourcesCAZypedia;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (349); Uniprot accession (13); PDB accession (15); 3D entries (6); cryst (0)
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 endo-α-1,5-L-arabinanase (BlAbn1; BLi03029; BL00353) 3.2.1.99 Bacillus licheniformis DSM 13 = ATCC 14580 AAU41895.1
AAU24536.1
Q65GB9
6B7K[A,B,C,D] 2.55
5
 endo-α-1,5-L-arabinanase (AbnA;BSU28810) (Arb43A) 3.2.1.99 Bacillus subtilis subsp. subtilis str. 168 CAA99586.1
AAV87172.1
AIY94210.1
AQR82908.1
AQR87123.1
CAB14841.1
CAB14841.2
NP_390759.1
A0A6M3ZJV7
P94522
1UV4[A] 1.50
5
 endo-α-1,5-L-arabinanase / exo-α-1,5-L-arabinanase 43A (ArbA; CJA_0805) (Arb43A) 3.2.1.99
3.2.1.-
Cellvibrio japonicus Ueda107 ACE84667.1
CAA71485.1
B3PKP8
P95470
1GYD[B] 2.05
1GYE[B] α-L-Araf-(1-5)-α-L-Araf
2.5
1GYH[A,B,C,D,E,F] 1.89
5
 α-1,5-L-arabinanase (AbnB;HUS_CDS25) (intracellular) 3.2.1.99 Geobacillus stearothermophilus T-6 NCIMB 40222 ACE73676.1 B3EYM8
3CU9[A] 1.06
3D5Y[A] 1.22
3D5Z[A] α-L-Araf-(1-5)-α-L-Araf-(1-5)-β-L-Araf
1.90
3D60[A] 1.90
3D61[A] α-L-Araf-(1-5)-β-L-Araf
1.95
6F1G[A] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf
1.67
5
 endo-α-1,5-L-arabinanase (Abn-ts) 3.2.1.99 Geobacillus thermodenitrificans TS-3 BAB64339.1 Q93HT9
1WL7[A] 1.90
6A8H[A] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf
1.65
6A8I[A,B] α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf
α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf
1.90
5
 exo-α-1,5-L-arabinanase (Arn3;ARN3) 3.2.1.- uncultured bacterium ADB43999.1 D2XML8
4KCB[A,B] 2.90
5
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