Glycoside Hydrolase Family 43 / Subf 4

Activities in Family	β-xylosidase (EC 3.2.1.37); α-L-arabinofuranosidase (EC 3.2.1.55); arabinanase (EC 3.2.1.99); xylanase (EC 3.2.1.8); galactan 1,3-β-galactosidase (EC 3.2.1.145); α-1,2-L-arabinofuranosidase (EC 3.2.1.-); exo-α-1,5-L-arabinofuranosidase (EC 3.2.1.-); [inverting] exo-α-1,5-L-arabinanase (EC 3.2.1.-); β-1,3-xylosidase (EC 3.2.1.-)
	Activities in Sub Family
Mechanism	Inverting
Clan	GH-F
3D Structure Status	5-fold β-propeller
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Glu
Note	Many members have been assigned to subfamilies as described by Mewis et al. (2016) Appl. Environm. Microbiol. in press (PMID: 26729713).
External resources	CAZypedia;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (441); Uniprot accession (83); PDB accession (14); 3D entries (5); cryst (0)

All (415) Bacteria (412) unclassified (3) Structure (5) Characterized (14)

Bacteria

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

Subf

BLi04220 / BL00219 (YxiA)

AAU43033.1
AAU25654.1
AAU25654.2
ACR87831.1
CAF05510.1
CAV28398.1

1.70

endo-α-1,5-L-arabinanase (Abn2;YxiA;J3A;BSU39330) (Arb43B)

CAB15969.2
ACB06752.1
AIY95249.1
AQR83975.1
AQR88190.1
CAB15969.1
NP_391812.1

endo-α-1,5-L-arabinanase (AbnA) (extracellular)

5HO0[A]		2.35
5HO2[A]		2.37
5HO9[A,B]	α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf	2.85
5HOF[A]	α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf-(1-5)-α-L-Araf	2.96
5HP6[A]		2.09

endo-α-1,5-L-arabinanase (AbnA;TpABN;Tpet_0637)

4KC7[A,B,C]		1.75
4KC8[A,B,C]		1.76

endo-α-1,5-L-arabinanase (Arn2;ARN2) (partial)

4KCA[A]		1.90
4KCA[B]		1.90