Activities in Sub Family	Exo-β-1,3-galactanase (EC 3.2.1.145);
Mechanism	Inverting e
Clan	GH-F
3D Structure Status	5-fold β-propeller
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Glu
Note	Many members have been assigned to subfamilies as described by Mewis et al. (2016) Appl. Environm. Microbiol. 82:1686-1692 (PMID: 26729713).
External resources	CAZypedia;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (987); Uniprot accession (8); PDB accession (16); 3D entries (6); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
[arabinogalactan] exo-β-1,3-galactanase (Ct1,3Gal43A; CtGH43; Cthe_0661) (1,3Gal43A)	3.2.1.145	Acetivibrio thermocellus ATCC 27405	ABN51896.1	A3DD67	3VSF[A,B,C,D,E,F] 2.76 3VSZ[A,B,C,D,E,F] β-D-Galp-(1-3)-β-D-Galp β-D-Galp-(1-3)-β-D-Galp-(1-3)-β-D-Galp 2.89 3VT0[A,B,C,D,E,F] β-D-Galp-(1-4)-β-D-Glcp 2.91 3VT1[A,B,C,D,E,F] β-D-Galp 3.19 3VT2[A,B,C,D,E,F] β-D-Galp1S-(1-1)-<non_carb> 3.00	24
BT2959 / BT_2959		Bacteroides thetaiotaomicron VPI-5482	AAO78065.1 NP_811871.1		3NQH[A] 2.11	24
exo-β-1,3-galactanase (BT0265;BT_0265)	3.2.1.145	Bacteroides thetaiotaomicron VPI-5482	AAO75372.1 NP_809178.1	Q8AB46	6EUF[A,B,C,D] α-L-Rhap-(1-4)-β-D-GlcpA-(1-6)[α-L-Araf-(1-4)][α-L-Araf-(1-3)]-β-D-Galp-(1-6)-β-D-Galp β-D-GlcpA-(1-6)[α-L-Araf-(1-3)][<non_carb>-(1-4)]-β-D-Galp-(1-6)-β-D-Galp β-D-GlcpA-(1-6)[α-L-Araf-(1-4)][α-L-Araf-(1-3)]-β-D-Galp-(1-6)-β-D-Galp 2.20 6EUJ[A,B,C,D] 2.75	24
β-1,3-galactosidase (BT3683)		Bacteroides thetaiotaomicron VPI-5482	AAO78788.1 NP_812594.1		6EUG[A] <carb_like_GIM> 1.61 6EUH[A,B,C] <carb_like_DGJ> 2.00 6EUI[A] β-D-Galp 1.76	24
exo-β-1,3-galactanase (BBIF_0009)		Bifidobacterium bifidum S17	ADO52214.1		5FLW[A,B] 1.40	24
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
exo-β-1,3-galactanase (Pc1,3Gal43A) (1,3Gal43A)	3.2.1.145	Phanerochaete chrysosporium	BAD98241.1	Q50KB2	7BYS[A,B] 1.40 7BYT[A] α-D-Galp β-D-Galp 1.50 7BYV[A] β-D-Galp β-D-Galp-(1-3)-β-D-Galp-(1-3)-β-D-Galp 2.50 7BYX[A,B,C,D] β-D-Galp-(1-3)-β-D-Galp-(1-3)-β-D-Galp 2.30	24
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