Glycoside Hydrolase Family 43 / Subf 24

Activities in Sub FamilyExo-β-1,3-galactanase (EC 3.2.1.145);
Mechanism Inverting e
ClanGH-F
3D Structure Status5-fold β-propeller
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorGlu
NoteMany members have been assigned to subfamilies as described by Mewis et al. (2016) Appl. Environm. Microbiol. 82:1686-1692 (PMID: 26729713).
External resourcesCAZypedia;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (1185); Uniprot accession (8); PDB accession (16); 3D entries (6); cryst (0)
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 [arabinogalactan] exo-β-1,3-galactanase (Ct1,3Gal43A; CtGH43; Cthe_0661) (1,3Gal43A) 3.2.1.145 Acetivibrio thermocellus ATCC 27405 ABN51896.1 A3DD67
3VSF[A,B,C,D,E,F] 2.76
3VSZ[A,B,C,D,E,F] β-D-Galp-(1-3)-β-D-Galp
β-D-Galp-(1-3)-β-D-Galp-(1-3)-β-D-Galp
2.89
3VT0[A,B,C,D,E,F] β-D-Galp-(1-4)-β-D-Glcp
2.91
3VT1[A,B,C,D,E,F] β-D-Galp
3.19
3VT2[A,B,C,D,E,F] β-D-Galp1S-(1-1)-<non_carb>
3.00
24
 BT2959 / BT_2959   Bacteroides thetaiotaomicron VPI-5482 AAO78065.1
NP_811871.1
 
3NQH[A] 2.11
24
 exo-β-1,3-galactanase (BT0265;BT_0265) 3.2.1.145 Bacteroides thetaiotaomicron VPI-5482 AAO75372.1
NP_809178.1
Q8AB46
6EUF[A,B,C,D] α-L-Rhap-(1-4)-β-D-GlcpA-(1-6)[α-L-Araf-(1-4)][α-L-Araf-(1-3)]-β-D-Galp-(1-6)-β-D-Galp
β-D-GlcpA-(1-6)[α-L-Araf-(1-3)][<non_carb>-(1-4)]-β-D-Galp-(1-6)-β-D-Galp
β-D-GlcpA-(1-6)[α-L-Araf-(1-4)][α-L-Araf-(1-3)]-β-D-Galp-(1-6)-β-D-Galp
2.20
6EUJ[A,B,C,D] 2.75
24
 β-1,3-galactosidase (BT3683)   Bacteroides thetaiotaomicron VPI-5482 AAO78788.1
NP_812594.1
 
6EUG[A] <carb_like_GIM>
1.61
6EUH[A,B,C] <carb_like_DGJ>
2.00
6EUI[A] β-D-Galp
1.76
24
 exo-β-1,3-galactanase (BBIF_0009)   Bifidobacterium bifidum S17 ADO52214.1  
5FLW[A,B] 1.40
24
Eukaryota
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 exo-β-1,3-galactanase (Pc1,3Gal43A) (1,3Gal43A) 3.2.1.145 Phanerochaete chrysosporium BAD98241.1 Q50KB2
7BYS[A,B] 1.40
7BYT[A] α-D-Galp
β-D-Galp
1.50
7BYV[A] β-D-Galp
β-D-Galp-(1-3)-β-D-Galp-(1-3)-β-D-Galp
2.50
7BYX[A,B,C,D] β-D-Galp-(1-3)-β-D-Galp-(1-3)-β-D-Galp
2.30
24
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