Glycoside Hydrolase Family 3

Activities in Familyβ-glucosidase (EC 3.2.1.21); xylan 1,4-β-xylosidase (EC 3.2.1.37); β-glucosylceramidase (EC 3.2.1.45); β-N-acetylhexosaminidase (EC 3.2.1.52); α-L-arabinofuranosidase (EC 3.2.1.55); glucan 1,3-β-glucosidase (EC 3.2.1.58); glucan 1,4-β-glucosidase (EC 3.2.1.74); isoprimeverose-producing oligoxyloglucan hydrolase (EC 3.2.1.120); coniferin β-glucosidase (EC 3.2.1.126); exo-1,3-1,4-glucanase (EC 3.2.1.-); β-N-acetylglucosaminide phosphorylases (EC 2.4.1.-)
Mechanism Retaining
Catalytic Nucleophile/BaseAsp (experimental);
Catalytic Proton DonorGlu for hydrolases (experimental); histidine for phosphorylases (experimental)
NoteA recent paper by Macdonald et al. (PMID=25533455) has shown that the enzymes that cleave b-hexosaminides are in fact retaining b-glycoside phosphorylases.
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX ;
Statistics GenBank accession (18870); Uniprot accession (2560); PDB accession (114); 3D entries (37); cryst (4)
All (18004) Archaea (96) Bacteria (16777) Eukaryota (1029) Viruses (1) unclassified (101) Structure (37 - 4 cryst) Characterized (298)
| 1 | ... | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3D
 β-glucosidase (Bgl3) (Bgl3A) 3.2.1.21 Talaromyces funiculosus NCL1 AFU91382.1    
 Bgl2 (fragment)   Talaromyces funiculosus NCL1 AFU91384.1    
 β-glucosidase (Bgl3A) 3.2.1.21 Talaromyces leycettanus JCM12802 AMR94085.1    
 β-glucosidase   Talaromyces piceae ATQ35958.1    
 β-glucosidase   Talaromyces piceae ATQ35961.1    
 β-glucosidase   Talaromyces piceae ATQ35957.1    
 β-glucosidase   Talaromyces piceae ATQ35964.1    
 β-glucosidase   Talaromyces piceae ATQ35956.1    
 β-glucosidase   Talaromyces piceae ATQ35960.1    
 β-glucosidase   Talaromyces piceae ATQ35965.1    
 β-glucosidase   Talaromyces piceae ATQ35959.1    
 β-xylosidase (Xyl2;XYL2)   Talaromyces purpureogenus ATCC MYA-38 AKH40279.1    
 β-glucosidase (Bgl3;Bgl) 3.2.1.21 Talaromyces purpureogenus KJS506 (KACC 93053P) ACV87737.1 C9E9M9  
 β-glucosidase (Bgl2;BGLII;Bg2;BGII) 3.2.1.21 Thermoascus aurantiacus IFO 9748 AAY33982.1
AAY33983.1
Q4U4W7  
 β-glucosidase (Bgl1;Bg1;TaBgl1) 3.2.1.21 Thermoascus aurantiacus IFO 9748 AAZ95587.1
AAZ95588.1
Q0ZUL0  
 β-xylosidase   Thermoascus aurantiacus QS 7-2-4 AMW64957.1    
 β-glucosidase   Thermoascus aurantiacus RCK 2011 AFU51372.1    
 β-1,4-glucosidase (Bgl2)   Thermoascus aurantiacus var. levisporus ABX56926.1 A9QUC3  
 β-1,4-glucosidase (Bgl2)   Thermoascus aurantiacus var. levisporus ABX56927.1 A9QUC4  
 β-1,4-glucosidase (Bgl1)   Thermoascus aurantiacus var. levisporus ABX79552.1
ABX79553.1
A9UFC6  
 MYCTH_62925   Thermothelomyces thermophila ATCC 42464 AEO53892.1    
 MYCTH_2059579   Thermothelomyces thermophila ATCC 42464 AEO56238.1    
 β-glucosidase (MYCTH_80304) 3.2.1.21
3.2.1.58
3.2.1.-
Thermothelomyces thermophila ATCC 42464 AEO58175.1    
 β-glucosidase (MtBgl3a;MYCTH_66804) (Bgl3A) 3.2.1.21 Thermothelomyces thermophila ATCC 42464 AEO58343.1    
 MYCTH_50705   Thermothelomyces thermophila ATCC 42464 AEO58346.1    
 MYCTH_2306784   Thermothelomyces thermophila ATCC 42464 AEO58968.1    
 MYCTH_104628   Thermothelomyces thermophila ATCC 42464 AEO60531.1    
 MYCTH_38200   Thermothelomyces thermophila ATCC 42464 AEO61246.1    
 MYCTH_104106   Thermothelomyces thermophila ATCC 42464 AEO61778.1    
 MYCTH_2308667 (fragment)   Thermothelomyces thermophila ATCC 42464 AEO59951.1    
 MYCTH_2129052 (fragment)   Thermothelomyces thermophila ATCC 42464 AEO59952.1    
 MYCTH_58882   Thermothelomyces thermophila ATCC 42464 AEO60477.1    
 THITE_2110159   Thielavia terrestris NRRL 8126 AEO64229.1    
 THITE_2110595   Thielavia terrestris NRRL 8126 AEO64446.1    
 THITE_2111169   Thielavia terrestris NRRL 8126 AEO64832.1    
 THITE_2113984   Thielavia terrestris NRRL 8126 AEO66128.1    
 THITE_2044936   Thielavia terrestris NRRL 8126 AEO67341.1    
 THITE_2144859   Thielavia terrestris NRRL 8126 AEO67512.1    
 THITE_2118136   Thielavia terrestris NRRL 8126 AEO68568.1    
 THITE_2119055   Thielavia terrestris NRRL 8126 AEO69052.1    
 THITE_122652   Thielavia terrestris NRRL 8126 AEO70559.1    
 THITE_2107118 (fragment)   Thielavia terrestris NRRL 8126 AEO62672.1    
 THITE_2107120 (fragment)   Thielavia terrestris NRRL 8126 AEO62673.1    
 THITE_2108759 (fragment)   Thielavia terrestris NRRL 8126 AEO63458.1    
 THITE_125239   Thielavia terrestris NRRL 8126 AEO68026.1    
 THITE_2031888 (short fragment)   Thielavia terrestris NRRL 8126 AEO69750.1    
 secreted protein   Thraustotheca clavata ATCC 34112 AIG55676.1    
 secreted protein   Thraustotheca clavata ATCC 34112 AIG55594.1    
 secreted protein (fragment)   Thraustotheca clavata ATCC 34112 AIG55725.1    
 secreted protein   Thraustotheca clavata ATCC 34112 AIG55751.1    
 β-glucosidase (TkBgl3A) (Bgl3A) 3.2.1.21 Trichoderma koningiopsis FCD3-1 AIB10099.1    
 β-xylosidase (Bxl1) (Xyl3A) 3.2.1.37 Trichoderma reesei CAA93248.1
CAW52645.1
CBC23920.1
Q92458 5A7M[A,B]
5AE6[A,B]
 TRIREDRAFT_79669 (Nag3)   Trichoderma reesei QM6a EGR47352.1    
 β-glucosidase (Cel3B)   Trichoderma reesei QM6A AAP57755.1
ABE16061.1
Q7Z9M5  
 Cel3c (Cel3C)   Trichoderma reesei QM6A AAP57756.1
ABH82050.1
Q7Z9M4  
 Cel3d (fragment) (Cel3D)   Trichoderma reesei QM6A AAP57759.1 Q7Z9M1  
 Cel3e (Cel3E)   Trichoderma reesei QM6A AAP57760.1
ACH26685.1
Q7Z9M0  
 β-glucosidase 1 (Bgl1;Bgl-1;TrBGL1) (Cel3A) 3.2.1.21 Trichoderma reesei QM9414 AAA18473.1 Q12715 3ZYZ[A]
4I8D[A,B]
 β-glucosidase (Cel3D)   Trichoderma reesei QM9414 BAP59014.1    
 β-glucosidase (Cel3G)   Trichoderma reesei QM9414 BAP59015.1    
 ORF   Trichoderma reesei QM9414 BAP59016.1    
 β-glucosidase   Trichoderma sp. SSL ACH92574.1 B5TYI5  
 β-glucosidase (epB-BGL) 3.2.1.21 Trichoderma sp. W63 / CGMCC 5209 AJC01624.1    
 β-N-acetylglucosaminidase (Nag3)   Trichoderma virens ACH72647.1 B5M694  
 xln   Trichoderma virens AMP44571.1    
 β-glucosidase I (Bgl1;BGL1;VbglI) 3.2.1.21 Trichoderma viride AS 3.3711 ACS93768.1 C6GGC9  
 β-glucosidase (Bgl1)   Trichoderma viride AS 3.3711 AAQ76093.1 Q6UJY0  
 β-glucosidase   Trigonella foenum-graecum AQY19164.1    
 β-glucosidase   Trigonella foenum-graecum AQY19164.1    
 β-D-glucan exohydrolase   Triticum aestivum AAM13694.1 Q8RWR5  
 unnamed protein product   Triticum aestivum CAW52652.1
CBC23934.1
CBD35273.1
   
 unnamed protein product   Triticum aestivum CAW52653.1
CBC23936.1
CBD35274.1
   
 ORF (fragment)   Triticum aestivum BAD06320.1 Q75RZ3  
 TRAES_3BF074800100CFD_c1   Triticum aestivum CDM84404.1    
 β-glucosidase 3.2.1.21 Tropaeolum majus CAA07070.1 O82074  
 β-glucosidase, partial (fragment)   uncultured fungus AKI33967.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33959.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33966.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33971.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33963.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33964.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33958.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33969.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33965.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33962.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33960.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33968.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33970.1    
 β-glucosidase, partial (fragment)   uncultured fungus AKI33961.1    
 β-glucosidase, partial (fragment)   uncultured fungus ARQ32329.1    
 β-glucosidase, partial (fragment)   uncultured fungus ARQ32330.1
ARU79426.1
   
 β-glucosidase, partial (fragment)   uncultured fungus ARQ32328.1    
 ORF (fragment)   uncultured fungus ARU79444.1    
 ORF (fragment)   uncultured fungus ARU79429.1    
 ORF (fragment)   uncultured fungus ARU79425.1    
 ORF (fragment)   uncultured fungus ARU79433.1    
 ORF (fragment)   uncultured fungus ARU79445.1    
 ORF (fragment)   uncultured fungus ARU79434.1    
 β-glucosidase (Bgl1) 3.2.1.21 Uromyces viciae-fabae CAE01320.1 Q70KQ7  
 UBRO_06098   Ustilago bromivora UB2112 SAM84689.1    
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