Glycoside Hydrolase Family 3

Activities in Familyβ-glucosidase (EC 3.2.1.21); xylan 1,4-β-xylosidase (EC 3.2.1.37); β-glucosylceramidase (EC 3.2.1.45); β-N-acetylhexosaminidase (EC 3.2.1.52); α-L-arabinofuranosidase (EC 3.2.1.55); glucan 1,3-β-glucosidase (EC 3.2.1.58); glucan 1,4-β-glucosidase (EC 3.2.1.74); isoprimeverose-producing oligoxyloglucan hydrolase (EC 3.2.1.120); coniferin β-glucosidase (EC 3.2.1.126); exo-1,3-1,4-glucanase (EC 3.2.1.-); β-N-acetylglucosaminide phosphorylases (EC 2.4.1.-)
Mechanism Retaining
Catalytic Nucleophile/BaseAsp (experimental);
Catalytic Proton DonorGlu for hydrolases (experimental); histidine for phosphorylases (experimental)
NoteA recent paper by Macdonald et al. (PMID=25533455) has shown that the enzymes that cleave b-hexosaminides are in fact retaining b-glycoside phosphorylases.
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX ;
Statistics GenBank accession (20155); Uniprot accession (2560); PDB accession (117); 3D entries (40); cryst (4)
All (19268) Archaea (102) Bacteria (18008) Eukaryota (1056) Viruses (1) unclassified (101) Structure (40 - 4 cryst) Characterized (300)
| 1 | ... | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | ... | 11 |
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3D
 ORF   Hypoxylon pulicicidum MF5954 AUM60063.1    
 β-glucosidase (BG)   Inonotus obliquus JL01 AMK37826.1    
 KLLA0D00330g   Kluyveromyces lactis NRRL Y-1140 CAH00182.1 Q6CSK3  
 KLLA0E25047g   Kluyveromyces lactis NRRL Y-1140 CAH00166.1 Q6CLW0  
 KLLA0E14674g   Kluyveromyces lactis NRRL Y-1140 CAG99696.1 Q6CN80  
 β-glucosidase   Kluyveromyces marxianus ATCC 12424 CAA29353.1 P07337  
 KLMA_30011   Kluyveromyces marxianus DMKU3-1042 BAO39306.1    
 KMAR_30004   Kluyveromyces marxianus NBRC 1777 BAP70809.1    
 β-glucosidase I (Bgl;KmBglI) 3.2.1.21 Kluyveromyces marxianus NBRC1777 ACY95404.1 D1GCC6 3ABZ[A,B,C,D]
3AC0[A,B,C,D]
 ATY40_BA7503307 (Bgli_aspfU)   Komagataella pastoris ATCC 28485 ANZ76010.1    
 AT249_GQ6704321 (Bgli_aspfU)   Komagataella phaffii ATCC 76273 AOA64421.1    
 BQ9382_C4-5255 (Acib2eukg773049)   Komagataella phaffii CBS 7435 SOP83950.1    
 PP7435_Chr4-1004 (Bgl7)   Komagataella phaffii CBS 7435 CCA41153.1    
 PAS_chr4_0013   Komagataella phaffii GS115 CAY71242.1
AOA69586.1
C4R6L7  
 β-glucosidase (BglN) 3.2.1.21 Kuraishia capsulata 35M5N AAA91297.1 Q12653  
 β-xylosidase / β-glucosidase (Lxyl-p1-1) 3.2.1.21
3.2.1.37
Lentinula edodes M95.33 AET31457.1
AET31458.1
   
 β-xylosidase / β-glucosidase (Lxyl-p1-2) (7-β-xylosyltaxane-active) 3.2.1.21
3.2.1.37
Lentinula edodes M95.33 AET31459.1
AET31460.1
   
 ORF   Leptosphaeria maculans JN3 CBX90257.1 E4ZG37  
 ORF   Leptosphaeria maculans JN3 CBX91338.1 E4ZJQ3  
 ORF   Leptosphaeria maculans JN3 CBX92329.1 E4ZLH0  
 ORF   Leptosphaeria maculans JN3 CBX93055.1 E4ZNJ6  
 ORF   Leptosphaeria maculans JN3 CBX93190.1 E4ZPG5  
 ORF   Leptosphaeria maculans JN3 CBX93517.1 E4ZPY2  
 ORF   Leptosphaeria maculans JN3 CBX95814.1 E4ZW38  
 ORF   Leptosphaeria maculans JN3 CBX97437.1 E5A1H3  
 ORF   Leptosphaeria maculans JN3 CBX99837.1 E5A892  
 ORF   Leptosphaeria maculans JN3 CBX90866.1 E4ZHN3  
 ORF   Leptosphaeria maculans JN3 CBX94846.1 E4ZTJ0  
 ORF (fragment)   Leptosphaeria maculans JN3 CBX95324.1 E4ZXB4  
 ORF (fragment)   Leptosphaeria maculans JN3 CBY00592.1 E5AAA1  
 β-glucosidase (Bgl1)   Leucoagaricus gongylophorus Ae322 CDJ79822.1    
 LRAMOSA06040   Lichtheimia ramosa CDS13866.1    
 LRAMOSA03589   Lichtheimia ramosa CDS11326.1    
 LRAMOSA03590   Lichtheimia ramosa CDS11327.1    
 LRAMOSA03599   Lichtheimia ramosa CDS11336.1    
 LRAMOSA04739   Lichtheimia ramosa CDS12545.1    
 LRAMOSA01544   Lichtheimia ramosa CDS07595.1    
 LRAMOSA02354   Lichtheimia ramosa CDS09677.1    
 LRAMOSA03168   Lichtheimia ramosa CDS10492.1    
 exo-1,3-β-glucanase (LP-ExoII)   Lilium longiflorum BAD13764.1 Q75Z80  
 exo-β-glucanase (ExoI)   Lilium longiflorum AAR14129.1 Q6UY81  
 β-glucan glucohydrolase (fragment)   Lolium perenne AFA36531.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50338.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50339.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50345.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50348.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50349.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50350.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50351.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50354.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50355.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50357.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50358.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50360.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50361.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50362.1    
 glycoside hydrolase family 3, partial (fragment)   Lophodermium sp. ZY-2014 AIJ50363.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50340.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50341.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50342.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50343.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50344.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50346.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50347.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50352.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50353.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50356.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50359.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50364.1    
 ORF (fragment)   Lophodermium sp. ZY-2014 AIJ50365.1    
 β-glucosidase 3.2.1.21 Magnaporthe grisea Y34 AAX07690.1 Q5EMW3  
 β-glucosidase (MoCel3B;MG03508.4) (Cel3B) 3.2.1.21 Magnaporthe oryzae 70-15 XP_360965.1 G4N7Z0  
 MG10038.4   Magnaporthe oryzae 70-15 XP_365193.1 G4N9A6  
 MG08250.4 (fragment)   Magnaporthe oryzae 70-15 XP_362798.1    
 MG10167.4   Magnaporthe oryzae 70-15 XP_365947.1    
 β-glucosidase (MoCel3A;MG09353.4) (Cel3A) 3.2.1.21 Magnaporthe oryzae 70-15 XP_364573.1 G4NI45  
 MG08985.4   Magnaporthe oryzae 70-15 XP_364140.1    
 MG08123.4   Magnaporthe oryzae 70-15 XP_362540.1    
 MG09272.4 (MoCel3F) (Cel3F)   Magnaporthe oryzae 70-15 XP_364427.1 G4MQA2  
 MG01396.4 (MoCel3D) (Cel3D)   Magnaporthe oryzae 70-15 XP_363470.1 G4MZH4  
 MGG_00621   Magnaporthe oryzae 70-15 XP_003718403.1
XP_368623.1
   
 MGG_12489   Magnaporthe oryzae 70-15 XP_003715843.1    
 MGG_11210   Magnaporthe oryzae 70-15 XP_003714391.1
XP_363514.1
XP_363515.1
   
 MGG_08583   Magnaporthe oryzae 70-15 XP_003716109.1
XP_362854.1
   
 MGG_01912   Magnaporthe oryzae 70-15 XP_003719348.1
EHA46981.1
XP_365210.1
   
 MGG_05599   Magnaporthe oryzae 70-15 XP_003710480.1
XP_360225.1
   
 MGG_01885   Magnaporthe oryzae 70-15 XP_003714903.1
XP_363958.1
   
 MGG_14903 (fragment)   Magnaporthe oryzae 70-15 XP_003719616.1
XP_361731.1
   
 MGG_07634 (fragment)   Magnaporthe oryzae 70-15 XP_003711559.1
XP_367723.1
   
 OOU_Y34scaffold00591g3   Magnaporthe oryzae Y34 ELQ37480.1
XP_364315.1
   
 OOU_Y34scaffold01031g15   Magnaporthe oryzae Y34 ELQ32819.1    
 MSYG_3929   Malassezia sympodialis ATCC 42132 SHO79579.1    
 α-L-arabinofuranosidase/β-D-xylosidase   Malus domestica ADI79208.1 D9D7L0  
 β-xylosidase / α-L-arabinosidase (Xyl1) 3.2.1.37
3.2.1.55
Medicago sativa subsp. x varia ABQ45227.1 A5JTQ2  
 β-xylosidase / α-L-arabinosidase (Xyl2)   Medicago sativa subsp. x varia ABQ45228.1 A5JTQ3  
 MtrDRAFT_AC148816g17v1 (fragment)   Medicago truncatula ABD28424.1    
 MtrDRAFT_AC148816g20v1   Medicago truncatula ABD28427.1 Q2HVM0  
 MtrDRAFT_AC152921g13v1 (fragment)   Medicago truncatula ABE78096.1    
 MtrDRAFT_AC145329g13v1 + MtrDRAFT_AC145329g14v1   Medicago truncatula ABE90987.1
ABE90988.1
ABP02856.1
ABP02857.1
   
 MtrDRAFT_AC148816g40v2   Medicago truncatula ABN05788.1 A2Q1G3  
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