Glycoside Hydrolase Family 3

Activities in Familyβ-glucosidase (EC; xylan 1,4-β-xylosidase (EC; β-glucosylceramidase (EC; β-N-acetylhexosaminidase (EC; α-L-arabinofuranosidase (EC; glucan 1,3-β-glucosidase (EC; glucan 1,4-β-glucosidase (EC; isoprimeverose-producing oligoxyloglucan hydrolase (EC; coniferin β-glucosidase (EC; exo-1,3-1,4-glucanase (EC 3.2.1.-); β-N-acetylglucosaminide phosphorylases (EC 2.4.1.-)
Mechanism Retaining
Catalytic Nucleophile/BaseAsp (experimental);
Catalytic Proton DonorGlu for hydrolases (experimental); histidine for phosphorylases (experimental)
NoteA recent paper by Macdonald et al. (PMID=25533455) has shown that the enzymes that cleave b-hexosaminides are in fact retaining b-glycoside phosphorylases.
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX ;
Statistics GenBank accession (20620); Uniprot accession (2560); PDB accession (117); 3D entries (40); cryst (4)
All (19731) Archaea (102) Bacteria (18469) Eukaryota (1058) Viruses (1) unclassified (101) Structure (40 - 4 cryst) Characterized (300)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ... | 11 |
Protein Name EC#OrganismGenBank UniprotPDB/3D
 An11g00200   Aspergillus niger CBS 513.88 CAK48253.1 A5ABF5  
 An11g06090 + An11g06080 (fragments)   Aspergillus niger CBS 513.88 CAK40763.1
 An14g01770   Aspergillus niger CBS 513.88 CAK46489.1 A2R2S3  
 An15g01890   Aspergillus niger CBS 513.88 CAK42292.1 A2R4X0  
 An15g04800   Aspergillus niger CBS 513.88 CAK42457.1 A2R5M1  
 An16g09090   Aspergillus niger CBS 513.88 CAK47105.1 A2R914  
 An17g00300   Aspergillus niger CBS 513.88 CAK97390.1 A2R967  
 An17g00520 (probable fragment)   Aspergillus niger CBS 513.88 CAK97412.1 A2R989  
 β-glucosidase (Bgl1;An18g03570) Aspergillus niger CBS 513.88 CAK48740.1 A2RAL4  
 β-glucosidase   Aspergillus niger CCTCC M2012129 AGC30596.1    
 β-glucosidase (Bgl1) Aspergillus niger CGMCC 3.316 AFJ19133.1    
 xylosidase XlnD   Aspergillus niger DSM 26641 ALN49269.1    
 β-xylosidase, partial (XlsiV)   Aspergillus niger E-1 BAX03547.1    
 ORF   Aspergillus niger E-1 BAX03548.1    
 β-glucosidase (fragment)   Aspergillus niger FTA008 ACJ64497.1 B7U4V8  
 β-xylosidase (XlnD) Aspergillus niger GS1 ADD69953.1    
 β-glucosidase   Aspergillus niger NIBGE ACN87968.1 C0M0K9  
 β-glucosidase   Aspergillus niger NIBGE 06 ABN73102.1 B0YIR9  
 β-glucosidase (Bgl)   Aspergillus niger NRRL 3135 CBA02054.1 C7C4Z9  
 β-glucosidase D, partial   Aspergillus niger TCCC41661 ANA68435.1    
 β-glucosidase   Aspergillus niger zju-2 ACJ02084.1 B6V747  
 β-glucosidase (Cel3) Aspergillus oryzae CAD67686.1
 β-xylosidase (XylA;Xyl-1) Aspergillus oryzae KBN616 BAA28267.1
 β-glucosidase (B7;BglA;Bgl1;Ao?G;BGHG1;BglC;AO090009000356) (Cel3A) Aspergillus oryzae RIB40 BAE54829.1 Q2UUD6 5FJJ[A,B,C,D]
 AO090009000554   Aspergillus oryzae RIB40 BAE54990.1 Q2UTX5  
 AO090005000337   Aspergillus oryzae RIB40 BAE55411.1 Q2USQ4  
 β-xylosidase (XylA;Xyl-1;AO090005000986) Aspergillus oryzae RIB40 BAE55977.1 Q2UR38  
 AO090001000266 (BglE)   Aspergillus oryzae RIB40 BAE56805.1 Q2UNR0  
 β-glucosidase 5 (Bgl5;BglG;AO090001000544) Aspergillus oryzae RIB40 BAE57053.1 Q2UN12  
 AO090003000741 (possible fragment)   Aspergillus oryzae RIB40 BAE57884.1 Q2UKN1  
 β-glucosidase 1 / aryl β-glucosidase (Bgl1;BglJ;AO090003001511) Aspergillus oryzae RIB40 BAE58551.1 Q2UIR4  
 AO090026000123   Aspergillus oryzae RIB40 BAE59617.1 Q2UFP8  
 AO090012000003   Aspergillus oryzae RIB40 BAE60251.1 Q2UDW4  
 AO090012000135 (BglB)   Aspergillus oryzae RIB40 BAE60358.1 Q2UDK7  
 AO090166000048   Aspergillus oryzae RIB40 BAE61700.1 Q2U9R5  
 AO090166000090   Aspergillus oryzae RIB40 BAE61738.1 Q2U9M7  
 AO090701000244   Aspergillus oryzae RIB40 BAE61980.1 Q2U8Y5  
 AO090701000274 (BglD)   Aspergillus oryzae RIB40 BAE62006.1 Q2U8V9  
 AO090701000841 (BglI)   Aspergillus oryzae RIB40 BAE62494.1 Q2U7H1  
 aryl β-glucosidase (BglA;AO090038000223) Aspergillus oryzae RIB40 BAE64040.1 Q2U325  
 β-1,6-glucosidase (BglH;AO090038000425) Aspergillus oryzae RIB40 BAE64214.1 Q2U2K1  
 AO090011000140   Aspergillus oryzae RIB40 BAE64689.1 Q2U176  
 AO090103000019   Aspergillus oryzae RIB40 BAE65506.1 Q2TZ17  
 AO090103000120   Aspergillus oryzae RIB40 BAE65591.1 Q2TYT2  
 aryl β-glucosidase (BglF;AO090103000127) Aspergillus oryzae RIB40 BAE65597.1 Q2TYS6  
 AO090010000034   Aspergillus oryzae RIB40 BAE65953.1 Q2TXS0  
 β-glucosidase (Bgl;BGL1) Aspergillus saccharolyticus CBS 127449 AEL79685.1    
 β-xylosidase (XlnD) Aspergillus sp. BCC125 AGB57183.1    
 Orf4   Aspergillus terreus AH-02-30-F7 AGC95327.1    
 β-glucosidase (BglS;ATEG_07931) Aspergillus terreus NIH2624 EAU32193.1 Q0CEF3  
 β-glucosidase   Aspergillus terreus SUK-1 ACY03273.1 D0VKF5  
 β-glucosidase 3 (fragment) Aspergillus wentii   P29090  
 β-1,4-xylosidase (XylI) Aureobasidium melanogenum ATCC 20524 BAI82526.1 D4AHT5  
 Bgl1   Bipolaris maydis C4 AAB84005.1 O13385  
 Bgl1   Bipolaris maydis C5 AAB82946.1 O13391  
 β-xylosidase B (AxylB)   Blastobotrys adeninivorans CUT08920.1    
 β-xylosidase A (AxylA)   Blastobotrys adeninivorans CUT08919.1    
 BCIN_10g05630   Botrytis cinerea B05.10 ATZ54571.1    
 BCIN_09g04850   Botrytis cinerea B05.10 ATZ53691.1    
 BCIN_06g04840   Botrytis cinerea B05.10 ATZ51038.1    
 BCIN_09g06990   Botrytis cinerea B05.10 ATZ53949.1    
 BCIN_08g06830   Botrytis cinerea B05.10 ATZ53072.1    
 BCIN_09g06990   Botrytis cinerea B05.10 ATZ53950.1    
 BCIN_10g05590   Botrytis cinerea B05.10 ATZ54567.1    
 BCIN_09g02640   Botrytis cinerea B05.10 ATZ53410.1    
 BCIN_15g02440   Botrytis cinerea B05.10 ATZ57693.1    
 BCIN_14g00480   Botrytis cinerea B05.10 ATZ56815.1    
 BCIN_10g05590   Botrytis cinerea B05.10 ATZ54566.1    
 BCIN_12g02830   Botrytis cinerea B05.10 ATZ55716.1    
 BCIN_05g07270   Botrytis cinerea B05.10 ATZ50372.1    
 BCIN_09g05460 (Bcsap1)   Botrytis cinerea B05.10 ATZ53770.1    
 BCIN_02g00420   Botrytis cinerea B05.10 ATZ46654.1    
 BCIN_03g08710   Botrytis cinerea B05.10 ATZ48686.1    
 BCIN_01g09960   Botrytis cinerea B05.10 ATZ46390.1    
 BCIN_09g06990   Botrytis cinerea B05.10 ATZ53947.1
 β-glucosidase / avenacinase (Sap1)
Botrytis cinerea SAS56 CAB61489.1 Q9UVJ6  
 glycoside hydrolase family 3 protein (Bofut4_p072500.1)   Botrytis cinerea T4 CCD33571.1    
 glycoside hydrolase family 3 protein (Bofut4_p068660.1)   Botrytis cinerea T4 CCD43026.1    
 glycoside hydrolase family 3 protein (Bofut4_p013340.1)   Botrytis cinerea T4 CCD43225.1    
 glycoside hydrolase family 3 protein (Bofut4_p053920.1)   Botrytis cinerea T4 CCD44491.1    
 glycoside hydrolase family 3 protein (Bofut4_p107060.1)   Botrytis cinerea T4 CCD48322.1    
 glycoside hydrolase family 3 protein (Bofut4_p025780.1)   Botrytis cinerea T4 CCD50179.1    
 glycoside hydrolase family 3 protein (Bofut4_p088840.1)   Botrytis cinerea T4 CCD50485.1    
 Bofut4_p086700.1   Botrytis cinerea T4 CCD50891.1    
 glycoside hydrolase family 3 protein (Bofut4_p082280.1)   Botrytis cinerea T4 CCD52069.1    
 glycoside hydrolase family 3 protein (Bofut4_p078070.1)   Botrytis cinerea T4 CCD52514.1    
 glycoside hydrolase family 3 protein (Bofut4_p141070.1)   Botrytis cinerea T4 CCD56845.1    
 glycoside hydrolase family 3 protein (Bofut4_p074410.1) (fragment)   Botrytis cinerea T4 CCD42639.1    
 glycoside hydrolase family 3 protein (Bofut4_p004830.1)   Botrytis cinerea T4 CCD47324.1    
 Bofut4_p087400.1 (Sap1) (fragment)   Botrytis cinerea T4 CCD50836.1    
 glycoside hydrolase family 3 protein (Bofut4_p086620.1)   Botrytis cinerea T4 CCD50883.1    
 glycoside hydrolase family 3 protein (Bofut4_p150540.1) (fragment)   Botrytis cinerea T4 CCD55922.1    
 unnamed protein product   Brassica napus CAW52650.1
 unnamed protein product   Brassica napus CAW52655.1
 unnamed protein product   Brassica napus CAW52654.1
 unnamed protein product   Brassica napus CAW52649.1
 β-glucosidase   Brettanomyces anomalus YV396 AKS48904.1    
 β-glucosidase   Brettanomyces bruxellensis YV397 AKS48905.1    
 Glycosyl hydrolase family 3   Caecomyces sp. Iso3 AWI66943.1    
 ORF (fragment)   Camellia sinensis ANQ46488.1    

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