Glycoside Hydrolase Family 3

Activities in Familyβ-glucosidase (EC 3.2.1.21); xylan 1,4-β-xylosidase (EC 3.2.1.37); β-glucosylceramidase (EC 3.2.1.45); β-N-acetylhexosaminidase (EC 3.2.1.52); α-L-arabinofuranosidase (EC 3.2.1.55); glucan 1,3-β-glucosidase (EC 3.2.1.58); glucan 1,4-β-glucosidase (EC 3.2.1.74); isoprimeverose-producing oligoxyloglucan hydrolase (EC 3.2.1.120); coniferin β-glucosidase (EC 3.2.1.126); exo-1,3-1,4-glucanase (EC 3.2.1.-); β-N-acetylglucosaminide phosphorylases (EC 2.4.1.-)
Mechanism Retaining
Catalytic Nucleophile/BaseAsp (experimental);
Catalytic Proton DonorGlu for hydrolases (experimental); histidine for phosphorylases (experimental)
NoteA recent paper by Macdonald et al. (PMID=25533455) has shown that the enzymes that cleave b-hexosaminides are in fact retaining b-glycoside phosphorylases.
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX ;
Statistics GenBank accession (17231); Uniprot accession (2560); PDB accession (105); 3D entries (33); cryst (4)
All (16380) Archaea (94) Bacteria (15236) Eukaryota (949) Viruses (1) unclassified (100) Structure (33 - 4 cryst) Characterized (293)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ... | 153 |
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D
 Arad_9829 (BglsF)   Agrobacterium radiobacter K84 ACM30787.1 B9JLQ1  
 B0909_20330   Agrobacterium rhizogenes K599 AQS64601.1    
 B0909_04485   Agrobacterium rhizogenes K599 AQS63165.1    
 AGROH133_06635   Agrobacterium sp. H13-3 ADY64638.1    
 BSY240_875   Agrobacterium sp. RAC06 AOG10948.1    
 BSY240_624 (BglB)   Agrobacterium sp. RAC06 AOG09514.1    
 coniferin β-glucosidase (Cbg1;Cbg-1) 3.2.1.21
3.2.1.126
Agrobacterium tumefaciens AAA22082.1 P27034  
 Ach5_16380   Agrobacterium tumefaciens Ach5 AKC07414.1    
 X971_1687   Agrobacterium tumefaciens LBA4213 (Ach5) AHK01564.1    
 AWN88_13505   Agrobacterium tumefaciens S33 AMD59291.1    
 Avi_0107   Agrobacterium vitis S4 ACM35056.1 B9JY26  
 Avi_2673   Agrobacterium vitis S4 ACM36922.1 B9JXE4  
 Avi_6257   Agrobacterium vitis S4 ACM39228.1 B9K2Y0  
 SAMN04489719_1319   Agrococcus carbonis DSM 22965 SDS00498.1    
 SAMN04489720_0891   Agrococcus jejuensis DSM 22002 SDH30777.1    
 SAMN04489720_1193   Agrococcus jejuensis DSM 22002 SDH41453.1    
 SAMN04489720_0909   Agrococcus jejuensis DSM 22002 SDH31152.1    
 ATC03_11050   Agromyces aureus AR33 ANJ27182.1    
 ATC03_09955   Agromyces aureus AR33 ANJ28837.1    
 ATC03_05190   Agromyces aureus AR33 ANJ26209.1    
 ATC03_05935   Agromyces aureus AR33 ANJ26323.1    
 ATC03_01080   Agromyces aureus AR33 ANJ28604.1    
 ATC03_05185   Agromyces aureus AR33 ANJ26208.1    
 ATC03_11045   Agromyces aureus AR33 ANJ27181.1    
 ATC03_10825   Agromyces aureus AR33 ANJ27144.1    
 ATC03_00340   Agromyces aureus AR33 ANJ25446.1    
 ATC03_18970   Agromyces aureus AR33 ANJ29083.1    
 SAMN04489721_2260   Agromyces flavus CPCC 202695 SDS97270.1    
 SAMN04489721_2000   Agromyces flavus CPCC 202695 SDS83745.1    
 SAMN04489721_2103   Agromyces flavus CPCC 202695 SDS89294.1    
 SAMN04489721_0700   Agromyces flavus CPCC 202695 SDS05457.1    
 SAMN04489721_1130   Agromyces flavus CPCC 202695 SDS32759.1    
 SAMN04489721_0302   Agromyces flavus CPCC 202695 SDR79552.1    
 PYTT_2532   Akkermansia glycaniphila SEI01009.1    
 PYTT_1900   Akkermansia glycaniphila SEH93924.1    
 PYTT_2056   Akkermansia glycaniphila SEH95655.1    
 Amuc_2109   Akkermansia muciniphila ATCC BAA-835 ACD05918.1 B2UPP0  
 A4V05_06480   Akkermansia muciniphila YL44 ANU61102.1
ASB34597.1
   
 CDA61_02005   Alcaligenes faecalis BDB4 ASC89223.1    
 AFA_11820   Alcaligenes faecalis JQ135 ASR90082.1    
 ALFP_2284   Alcaligenes faecalis P156 ARP54171.1    
 UZ73_02215   Alcaligenes faecalis ZD02 ALO37180.1    
 ABO_1018   Alcanivorax borkumensis SK2 CAL16466.1 Q0VQT2  
 B5T_01825 (NagZ)   Alcanivorax dieselolei B5 MCCC 1A00001 AFT70100.1    
 S7S_10150   Alcanivorax pacificus W11-5 AJD48442.1    
 CEK62_04440   Alcanivorax sp. N3-7A ASK36418.1    
 AS19_10830   Alcanivorax sp. NBRC 101098 BAP13934.1    
 P40_08695   Alcanivorax xenomutans P40 ARB45486.1    
 APS56_03630   Algibacter alginicilyticus HZ22 ALJ04285.1    
 APS56_05915   Algibacter alginicilyticus HZ22 ALJ06750.1    
 APS56_06025   Algibacter alginicilyticus HZ22 ALJ06754.1    
 APS56_03015   Algibacter alginicilyticus HZ22 ALJ04179.1    
 APS56_02980   Algibacter alginicilyticus HZ22 ALJ06705.1    
 AO498_15075   Algoriphagus sp. M8-2 AMQ57775.1    
 AO498_00075   Algoriphagus sp. M8-2 AMQ54768.1    
 AO498_15615   Algoriphagus sp. M8-2 AMQ57881.1    
 AO498_08400   Algoriphagus sp. M8-2 AMQ56433.1    
 AO498_01080   Algoriphagus sp. M8-2 AMQ54964.1    
 Alide_3168   Alicycliphilus denitrificans BC ADV00890.1 D6VJ78  
 Alide2_1279   Alicycliphilus denitrificans K601 AEB83683.1    
 Aaci_0332   Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 ACV57391.1 C8WRI6  
 Aaci_2630   Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 ACV59634.1 C8WTP2  
 TC41_0453 (BglX)   Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1 AEJ42419.1    
 β-xylosidase (BglX;TC41_2927) 3.2.1.37 Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1 AEJ44817.1    
 glycosyl hydrolase   Alicyclobacillus sp. A4 AMC37202.1    
 VSAL_II0575 (NagZ)   Aliivibrio salmonicida LFI1238 CAQ81329.1 B6ERJ6  
 pVSAL840_55   Aliivibrio salmonicida LFI1238 CAQ81914.1 B6ET26  
 AWOD_II_0544 (NagZ)   Aliivibrio wodanis CED57185.1    
 Alfi_1075   Alistipes finegoldii DSM 17242 AFL77429.1    
 Alfi_1072   Alistipes finegoldii DSM 17242 AFL77426.1    
 Alfi_0076   Alistipes finegoldii DSM 17242 AFL76492.1    
 Alfi_1076   Alistipes finegoldii DSM 17242 AFL77430.1    
 Alfi_1590   Alistipes finegoldii DSM 17242 AFL77929.1    
 Alfi_2660   Alistipes finegoldii DSM 17242 AFL78920.1    
 Alfi_1610   Alistipes finegoldii DSM 17242 AFL77944.1    
 AL1_02170   Alistipes shahii WAL 8301 CBK62920.1 D4IJ08  
 AL1_13740   Alistipes shahii WAL 8301 CBK63828.1 D4ILL6  
 AL1_13780   Alistipes shahii WAL 8301 CBK63832.1 D4ILM0  
 AL1_15910   Alistipes shahii WAL 8301 CBK63996.1 D4IM34  
 AL1_11640   Alistipes shahii WAL 8301 CBK63660.1 D4IL48  
 AL1_13770   Alistipes shahii WAL 8301 CBK63831.1 D4ILL9  
 Mlg_1327   Alkalilimnicola ehrlichii MLHE-1 ABI56676.1 Q0A911  
 Amet_1301   Alkaliphilus metalliredigens QYMF ABR47506.1 A6TMT8  
 Clos_2373   Alkaliphilus oremlandii OhILAs ABW19905.1 A8MJC3  
 CDL62_00090   Alkalitalea saponilacus DSM 24412 ASB50990.1    
 CDL62_10005   Alkalitalea saponilacus DSM 24412 ASB49446.1    
 CDL62_05050   Alkalitalea saponilacus DSM 24412 ASB48548.1    
 CDL62_00100   Alkalitalea saponilacus DSM 24412 ASB47665.1    
 CDL62_11970   Alkalitalea saponilacus DSM 24412 ASB49798.1    
 CDL62_06305   Alkalitalea saponilacus DSM 24412 ASB48771.1    
 CDL62_10000   Alkalitalea saponilacus DSM 24412 ASB49445.1    
 ORF   Alloactinosynnema sp. L-07 CRK57453.1    
 ORF   Alloactinosynnema sp. L-07 CRK61072.1    
 ORF   Alloactinosynnema sp. L-07 CRK62215.1    
 Alvin_0490   Allochromatium vinosum DSM 180 ADC61447.1 D3RNT7  
 SD28_05140   Allofrancisella guangzhouensis 08HL01032 AJC49060.1    
 SAMN04489726_6997   Allokutzneria albata DSM 44149 SDN52292.1    
 SAMN04489726_7597   Allokutzneria albata DSM 44149 SDN65388.1    
 SAMN04489726_5736   Allokutzneria albata DSM 44149 SDN25405.1    
 SAMN04489726_1433   Allokutzneria albata DSM 44149 SDM40043.1    
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