Glycoside Hydrolase Family 36

Known Activities	α-galactosidase (EC 3.2.1.22); α-N-acetylgalactosaminidase (EC 3.2.1.49); stachyose synthase (EC 2.4.1.67); raffinose synthase (EC 2.4.1.82)
Mechanism	Retaining
Clan	GH-D
3D Structure Status	( β / α ) ₈
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Asp (experimental)
Note	Mechanistic commonality with family GH27 demonstrated by Comfort et al. (2007) Biochemistry, 46: 3319-3330 (PMID: 17323919)
External resources	CAZypedia; InterPro; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME;
Statistics	GenBank accession (1001); Uniprot accession (559); PDB accession (6); 3D entries (6); cryst (2)

Summary

All (839) Archaea (21) Bacteria (699) Eukaryota (119) Structure (6 - 2 cryst) Characterized (55)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
α-galactosidase (SSO3127)	3.2.1.22	Sulfolobus solfataricus P2	AAK43227.1 CAC24100.1 NP_344437.1	Q97U94
α-galactosidase (ST2554)	3.2.1.22	Sulfolobus tokodaii str. 7	BAB67666.1 BAK54821.1 NP_378557.1	Q96XG2
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
α-galactosidase (Aga)	3.2.1.22	Bifidobacterium adolescentis DSM 20083	AAD30994.1 AAD30994.2	Q9XCX2
α-galactosidase (MelA)	3.2.1.22	Bifidobacterium bifidum NCIMB 41171	ABD96085.1	Q1KTD9
α-galactosidase (Aga)	3.2.1.22	Bifidobacterium breve 203	AAK96217.2	Q93CM6
α-galactosidase (Aga2)	3.2.1.22	Bifidobacterium breve 203	ABB76662.1	Q2XQ11
α-galactosidase I (GalA1;BLD_1483)	3.2.1.22	Bifidobacterium longum DJO10A	ACD98928.1	B3DP69
α-galactosidase I (AgA;BL1518)	3.2.1.22	Bifidobacterium longum NCC2705	AAN25312.1 NP_696676.1
α-galactosidase (AglL)	3.2.1.22	Bifidobacterium longum VMKB44	AAG02023.1	Q9F9W6
α-galactosidase (AgaA)	3.2.1.22	Carnobacterium maltaromaticum BA	AAL27305.1	Q93DW8
α-N-acetylgalactosaminidase (AagA)	3.2.1.49	Clostridium perfringens ATCC 10543	AAM55479.1 ABI01263.1	Q8XNK8
α-galactosidase (Aga36A) (Aga36A)	3.2.1.22	Clostridium stercorarium F-9	BAC56887.1	Q84IQ0
α-galactosidase (RafA;Dda3937_03029)	3.2.1.22	Dickeya dadantii 3937	ADM97865.1	E0SII4
α-galactosidase (AgaB;AgaN;EfmE980_0219)	3.2.1.22	Enterococcus faecium E980	EFF38777.1	D4QHE9
α-galactosidase (RafA)	3.2.1.22	Escherichia coli K-12 MG1655	AAA24497.1	P16551.1
α-galactosidase (GalA17)	3.2.1.22	Flavobacterium sp. TN17	ADK91093.1	E5DWL9
α-galactosidase (AgaB;AgaB1)	3.2.1.22	Geobacillus stearothermophilus KVE39	AAG49421.1	Q934H7	cryst
α-galactosidase (AgaA;Aga1)	3.2.1.22	Geobacillus stearothermophilus KVE39	AAG49420.1	Q9ALJ4	cryst
α-galactosidase (AgaN)	3.2.1.22	Geobacillus stearothermophilus NUB3621	AAD23585.1	Q9X624
α-galactosidase (MelA;Aga;LBA1438)	3.2.1.22	Lactobacillus acidophilus NCFM	AAV43263.1		2XN0[A,B] 2XN1[A,B,C,D] 2XN2[A]
α-galactosidase (MelA)	3.2.1.22	Lactobacillus fermentum CRL722	AAT37673.1	Q6IYF5
α-galactosidase (MelA)	3.2.1.22	Lactobacillus plantarum ATCC 8014	AAF02774.3	Q9L905
α-galactosidase (MelA)	3.2.1.22	Lactobacillus plantarum DSM 20205	CAI60220.1	Q5DU70
α-galactosidase (Aga)	3.2.1.22	Lactococcus raffinolactis ATCC 43920	AAM18789.1 AAO26321.1 CAD60129.1	Q7X1P3
α-galactosidase (AGA)	3.2.1.22	Leuconostoc mesenteroides SY1	AAU94931.1	Q5XLI6
α-galactosidase (Agα-MJ11)	3.2.1.22	Pedobacter nyackensis MJ11 CGMCC 2503	ACN78884.1	D2DFB2
α-galactosidase	3.2.1.22	Pseudoalteromonas sp. KMM 701 (VKM B-2135 D)	ABF72189.1	Q19AX0
α-galactosidase/sucrose kinase (AgaSK;RUGNEv3_61222)	3.2.1.22	Ruminococcus gnavus E1	CCA61959.1		2YFO[A]
α-galactosidase (Aga)	3.2.1.22	Streptococcus mutans INGBRITT	AAA26933.1	P27756
α-galactosidase (SCO0274;SCF85.02)	3.2.1.22	Streptomyces coelicolor A3(2)	CAB54159.1 NP_624603.1	Q9S2D9
α-galactosidase (Aga)	3.2.1.22	Streptomyces sp. S27 ACCC 41168	ACN78885.1 ADK91095.1	D2DFB3
α-galactosidase (GalA;Tm1192) (Gal36)	3.2.1.22	Thermotoga maritima MSB8	AAD36267.1 AAM56791.1 CAA04514.1 CAD29026.1 CAH68692.1 CAT81453.1 NP_228997.1	O33835	1ZY9[A]
α-galactosidase (AglA)	3.2.1.22	Thermotoga neapolitana 5068	AAC38607.1	Q9R7H1
α-galactosidase (AgaT)	3.2.1.22	Thermus brockianus ITI360	AAD33668.1	Q9X6C7
α-galactosidase (AglA)	3.2.1.22	Thermus sp. T2	BAA76597.1 CAB65477.1	Q9WXC1
α-galactosidase (AgaT)	3.2.1.22	Thermus thermophilus TH125	AAD32628.1	Q9X6D2
α-galactosidase (AgaY;Agα-Y;GalA;YP1469)	3.2.1.22	Yersinia pestis biovar Microtus str. 91001	AAS61708.1 ACH90515.1 NP_992831.1
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
alkaline α-galactosidase (Sip2;AtSip2;At3g57520)	3.2.1.22	Arabidopsis thaliana	AAK92707.1 AEE79665.1 AEE79666.1 AEE79667.1 BAE98518.1 CAB66109.1 NP_191311.1	Q94A08 Q9SCM1
α-galactosidase (AglC;AN8138.2)	3.2.1.22	Aspergillus nidulans FGSC A4	ABF50891.1 EAA58775.1	C8V6Q1
α-galactosidase C / I (AglC)	3.2.1.22	Aspergillus niger CBS 120.49 / N400	CAB63901.1	Q9UUZ4
α-galactosidase (seed imbibition protein;Sip) (fragment)	3.2.1.22	Cicer arietinum	CAA65125.1	Q39466
alkaline α-galactosidase I (Aga1)	3.2.1.22	Cucumis melo	AAM75139.1	Q84NI7
alkaline α-galactosidase II (Aga2)	3.2.1.22	Cucumis melo	AAM75140.1	Q84NI6
α-galactosidase (Agα-F75)	3.2.1.22	Gibberella sp. F75	ACQ72829.1 ACS12899.1	C6FJG8
α-galactosidase 2 / II (Agl2;AGLII)	3.2.1.22	Hypocrea jecorina RUTC-30	CAA93245.1	Q92457
α-galactosidase	3.2.1.22	Lichtheimia corymbifera IFO 8084	AAF68953.1 ABB43117.1	Q9P8N4
raffinose synthase (P0583G08.2;OJ1276_B06.31;OJ1276_B06.38)	2.4.1.82	Oryza sativa Japonica Group	BAB64768.1 BAB92522.1 BAD68247.1 BAD68321.1 BAF04051.1
alkaline α-galactosidase (OsAkaGal;Osh69;Os08g0495800)	3.2.1.22	Oryza sativa Japonica Group	AAL65392.2 BAD10121.1 BAD10122.1 BAF24047.1	Q8W2G5
α-galactosidase II (AgalII) (peptide fragment)	3.2.1.22	Penicillium simplicissimum VTT-D-78090
α-galactosidase (Agl1)	3.2.1.22	Penicillium sp. F63 CGMCC1669	ABC70181.1	A4Z4V0
raffinose synthase (Rfs)	2.4.1.82	Pisum sativum	CAD20127.2	Q8VWN6
stachyose synthase (Sts1)	2.4.1.67	Pisum sativum	CAC38094.1 CAD55555.1	Q93XK2
α-galactosidase (Agα-F78)	3.2.1.22	Rhizopus sp. ACCC 30795 F78	ACM48349.1	C7SEV1
α-galactosidase (peptide fragment)	3.2.1.22	Thermomyces lanuginosus CBS 395.62/B
stachyose synthase	2.4.1.67	Vigna angularis	CAB64363.1	Q9SBZ0

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.