Activities in Family	sialidase or neuraminidase (EC 3.2.1.18); trans-sialidase (EC 2.4.1.-); anhydrosialidase (EC 4.2.2.15); Kdo hydrolase (EC 3.2.1.-); 2-keto-3-deoxynononic acid hydrolase / KDNase (EC 3.2.1.-)
Mechanism	Retaining
Clan	GH-E
3D Structure Status	6-fold β-propeller
Catalytic Nucleophile/Base	Tyr+Glu
Catalytic Proton Donor	Not known
External resources	HOMSTRAD;
Commercial Enzyme Provider(s)	MEGAZYME;
Statistics	GenBank accession (7303); Uniprot accession (682); PDB accession (159); 3D entries (21); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
Sialidase (Hz136)	3.2.1.18	Alistipes			6MNJ[B] 3.35 6MNJ[A] 3.35
BACCAC_01090 (23295)		Bacteroides caccae ATCC 43185	ZP_01959484.1	A5ZDY0	4Q6K[A,B,C,D] 2.00
sialidase (BtsA;BTSA;BT0455)	3.2.1.18	Bacteroides thetaiotaomicron VPI-5482	AAO75562.1 NP_809368.1	Q8AAK9	4BBW[A] 2.30
sialidase (CpNanI;CPF_0721)	3.2.1.18	Clostridium perfringens ATCC 13124	ABG83208.1	Q0TT67	4L2E[A] 2.30 5TSP[A,B] 2.00
exo-α-sialidase (NanI;CPSA;CPE0725)	3.2.1.18	Clostridium perfringens str. 13	BAB80431.1 NP_561641.1	Q8XMG4	2BF6[A] α-D-Neup5Ac 2.09 2VK5[A] 1.80 2VK6[A] <carb_like_DAN> 1.10 2VK7[A,B] α-L-Manp3F5Ac-(2-0)-TYR 1.10
sialidase (NedA)	3.2.1.18	Micromonospora viridifaciens	BAA00852.1 AAQ70871.1	Q02834	1EUR[A] 1.85 1EUS[A] <carb_like_DAN> 2.50 1EUT[A] 3.00 1EUU[A] β-D-Galp 2.00 1W8N[A] β-D-Galp <carb_like_DAN> 1.62 1W8O[A] β-D-Galp-(1-4)-α-D-Glcp 1.30 1WCQ[A,B,C] <carb_like_DAN> 2.30 2BER[A] β-D-Neup5Ac 1.45 2BQ9[A,B,C] β-D-Galp 2.20 2BZD[A,B,C] β-D-Galp 2.00 4J9T[A] 2.30
BDI_2946		Parabacteroides distasonis ATCC 8503	ABR44654.1	A6LG40	4FJ6[A] 1.50
anhydrosialidase (RgNanH;HMPREF1201_01421) (intramolecular)	4.2.2.15	Ruminococcus gnavus	ETD19277.1		4X47[A] <carb_like_DAN> 2.60 4X49[A] <carb_like_G39> 1.85 4X4A[A] 2.35 4X6K[A] 2.10
sialidase (NanH;STSA)	3.2.1.18	Salmonella typhimurium TA262	AAA27168.1	P29768	1DIL[A] L-1,2,3,4-deoxy-Ribp <carb_like_AXP> 2.25 1DIM[A] 1.60 2SIL[A] 1.30 2SIM[A] <carb_like_DAN> 2.0 3SIL[A] 2.20 6TRG[XXX] 6TVI[AAA] 7AEY[AAA] 7AF2[AAA]
sialidase A (NanA)	3.2.1.18	Streptococcus pneumoniae 6	CAA51473.1 ACJ76903.1	B8XTT3 P62575 Q59959	5KKY[A,B] 2.30 7A54[A] <carb_like_DAN> 2.20 7A5X[A] 2.30
neuraminidase (NanC)	3.2.1.18	Streptococcus pneumoniae G54	ACF56230.1	B5E561	4YW0[A,B] 1.48 4YW1[A,B] α-D-Neup5Ac <carb_like_DAN> 1.27 4YW2[A,B] α-D-Neup5Ac-(2-6)-β-D-Galp 2.00 4YW3[A,B] α-D-Neup5Ac <carb_like_DAN> 1.98 4YW4[A,B] 2.10 4YW5[A,B] <carb_like_G39> 1.90 4YZ1[A,B] 2.18 4YZ2[A,B] <carb_like_DAN> 3.20 4YZ3[A,B] <carb_like_G39> 3.00 4YZ4[A,B] α-D-Neup5Ac 1.58 4YZ5[A,B] α-D-Neup5Ac-(2-3)-β-D-Galp <carb_like_DAN> 2.00 5F9T[A,B] α-L-Manp3F5Ac-(2-0)-TYR 2.30
neuraminidase (NanC)	3.2.1.18	Streptococcus pneumoniae G54	ACF56230.1	B5E561	4YW0[A,B] 1.48 4YW1[A,B] α-D-Neup5Ac <carb_like_DAN> 1.27 4YW2[A,B] α-D-Neup5Ac-(2-6)-β-D-Galp 2.00 4YW3[A,B] α-D-Neup5Ac <carb_like_DAN> 1.98 4YW4[A,B] 2.10 4YW5[A,B] <carb_like_G39> 1.90 4YZ1[A,B] 2.18 4YZ2[A,B] <carb_like_DAN> 3.20 4YZ3[A,B] <carb_like_G39> 3.00 4YZ4[A,B] α-D-Neup5Ac 1.58 4YZ5[A,B] α-D-Neup5Ac-(2-3)-β-D-Galp <carb_like_DAN> 2.00 5F9T[A,B] α-L-Manp3F5Ac-(2-0)-TYR 2.30
sialidase A (NanA)		Streptococcus pneumoniae R6	AAL00340.1 ACJ76904.1 NP_359129.1	P62576	2VVZ[A,B] <carb_like_DAN> 2.00 2W20[A,B] 2.48 3H71[A,B] 1.80 3H72[A,B] α-D-Neup5Ac 1.80 3H73[A,B] <carb_like_DAN> 2.30
intramolecular trans-sialidase B (NanB;SP_1687)	4.2.2.15	Streptococcus pneumoniae TIGR4	AAK75766.1 NP_346126.1	Q54727	2JKB[A] 2.00 2VW0[A] 3.90 2VW1[A] <carb_like_DAN> 2.12 2VW2[A] 1.90 4FOQ[A] 1.40 4FOV[A] 2.28 4FOW[A] 1.70 4FOY[A] 1.80 4FP2[A] 2.14 4FP3[A] 1.85 4FPC[A] 1.80 4FPE[A] 1.79 4FPF[A] 1.80 4FPG[A] 2.10 4FPH[A] 2.80 4FPJ[A] 1.80 4FPK[A] 1.62 4FPL[A] 2.00 4FPO[B] 2.90 4FPY[A] 2.00 4FQ4[A] 1.48 4XE9[A] 3.30 4XHB[A] 2.26 4XHX[A] 2.15 4XIK[A] 1.80 4XIL[A] 1.50 4XIO[A] 1.80 4XJ8[A] 1.20 4XJ9[A] 1.6 4XJA[A] 2.00 4XJU[A] 2.15 4XJW[A] 2.30 4XJZ[A] 1.50 4XMA[A] 1.31 4XMI[A] 1.20 4XOG[A] <carb_like_DAN> 1.95 4XYX[A] 2.40
intramolecular trans-sialidase B (NanB;SP_1687)	4.2.2.15	Streptococcus pneumoniae TIGR4	AAK75766.1 NP_346126.1	Q54727	2JKB[A] 2.00 2VW0[A] 3.90 2VW1[A] <carb_like_DAN> 2.12 2VW2[A] 1.90 4FOQ[A] 1.40 4FOV[A] 2.28 4FOW[A] 1.70 4FOY[A] 1.80 4FP2[A] 2.14 4FP3[A] 1.85 4FPC[A] 1.80 4FPE[A] 1.79 4FPF[A] 1.80 4FPG[A] 2.10 4FPH[A] 2.80 4FPJ[A] 1.80 4FPK[A] 1.62 4FPL[A] 2.00 4FPO[B] 2.90 4FPY[A] 2.00 4FQ4[A] 1.48 4XE9[A] 3.30 4XHB[A] 2.26 4XHX[A] 2.15 4XIK[A] 1.80 4XIL[A] 1.50 4XIO[A] 1.80 4XJ8[A] 1.20 4XJ9[A] 1.6 4XJA[A] 2.00 4XJU[A] 2.15 4XJW[A] 2.30 4XJZ[A] 1.50 4XMA[A] 1.31 4XMI[A] 1.20 4XOG[A] <carb_like_DAN> 1.95 4XYX[A] 2.40
neuraminidase (NanA;SpneT_02001817)		Streptococcus pneumoniae TIGR4	ZP_01407762.1		2YA4[A,B] 2.70 2YA5[A,B] α-D-Neup5Ac 2.45 2YA6[A,B] <carb_like_DAN> 2.18 2YA7[A,B,C,D] <carb_like_ZMR> 1.90 2YA8[A,B] <carb_like_G39> 1.85
Sialidase26		unidentified bacterium			6MRV[A] <carb_like_DAN> 2.10 6MRX[A,B,C,D] 1.70 6MYV[A,B,C,D] 1.85 6MRV[B] <carb_like_DAN> 2.10
sialidase / neuraminidase (NanH;VcNA;VCSA)	3.2.1.18	Vibrio cholerae 569B 395	AAA27546.1	P37060	1KIT[A] 1.96 1W0O[A] α-D-Neup5Ac <carb_like_DAN> 2.50 1W0P[A] α-D-Neup5Ac 1.80 6EKS[A] <carb_like_G39> 1.90 6EKU[A] <carb_like_ZMR> 1.90
sialidase / neuraminidase (NanH;VcNA;VCSA)	3.2.1.18	Vibrio cholerae 569B 395	AAA27546.1	P37060	1KIT[A] 1.96 1W0O[A] α-D-Neup5Ac <carb_like_DAN> 2.50 1W0P[A] α-D-Neup5Ac 1.80 6EKS[A] <carb_like_G39> 1.90 6EKU[A] <carb_like_ZMR> 1.90
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
2-keto-3-deoxynononic acid sialidase (KDNase;AfKDNase;Afu4g13800;AFUA_4G13800) (6407)	3.2.1.18 3.2.1.-	Aspergillus fumigatus Af293	EAL89414.2		2XCY[A,B] 1.80 2XZI[A,B] α-D-Neup 1.90 2XZJ[A,B] 2.00 2XZK[A,B] α-L-Manp3F-(2-0)-TYR <carb_like_K99> 2.20 4M4N[A,B] 2.15 4M4U[A,B] 1.20 4M4V[A,B] <carb_like_DAN> 2.40
sialidase (Neu2) (cytosolic)	3.2.1.18	Homo sapiens	CAB41449.1 AAH69151.1 AAI07054.1 AAY24360.1 NP_005374.1	Q9Y3R4	1SNT[A] 2.40 1SO7[A] 2.30 1VCU[A,B] <carb_like_DAN> 2.30 2F0Z[A] <carb_like_ZMR> 2.40 2F10[A] <carb_like_BCZ> 1.47 2F11[A] β-D-4-deoxy-GlcpANAc-(1-1)-<non_carb> 2.19 2F12[A] β-D-4-deoxy-GlcpANAc-(1-1)-<non_carb> 1.76 2F13[A] α-L-4-deoxy-IdopANAc-(1-1)-<non_carb> 2.10 2F24[A] 2.68 2F25[A,B] <carb_like_DAN> 1.65 2F26[A] 2.30 2F27[A,B] <carb_like_DAN> 2.10 2F28[A] 2.25 2F29[A,B] <carb_like_DAN> 2.05 4NC5[A] α-L-Manp3F5Ac-(2-0)-TYR 1.8 4NCS[A] α-L-Glcp3F5Ac-(2-0)-TYR 1.89
intramolecular trans-sialidase L (MDSA)	4.2.2.15	Macrobdella decora	AAC47263.1	Q27701	1SLI[A] <carb_like_DAN> 1.80 1SLL[A] 3.00 2SLI[A] 2.30 3SLI[A] 2.00 4SLI[A] <carb_like_CNP> 2.85
intramolecular trans-sialidase L (MDSA)	4.2.2.15	Macrobdella decora	AAC47263.1	Q27701	1SLI[A] <carb_like_DAN> 1.80 1SLL[A] 3.00 2SLI[A] 2.30 3SLI[A] 2.00 4SLI[A] <carb_like_CNP> 2.85
trans-sialidase (TcTS)	2.4.1.-	Trypanosoma cruzi	AAA66352.1 AAO30670.1 AAT21542.1	Q26966	1MR5[A] 1.97 1MS0[A,B] β-D-Galp-(1-4)-α-D-Glcp <carb_like_DAN> 2.61 1MS1[A,B] <carb_like_DAN> 1.95 1MS3[A,B] NMR 1MS4[A,B] NMR 1MS5[A,B] 1.70 1MS8[A,B] <carb_like_DAN> 1.50 1MS9[A,B] β-D-Galp-(1-4)-β-D-Glcp 2.70 1S0I[A] α-D-Neup5Ac-(2-3)-β-D-Galp-(1-4)-α-D-Glcp 1.30 1S0J[A] α-D-Neup5Ac-(2-1)-<non_carb> 1.75 1S0K[A] α-L-Manp3F5Ac-(2-0)-TYR NMR 2AH2[A] α-L-Manp3F5Ac-(2-0)-TYR 2.64 3B69[A] α-L-Manp3F5Ac9N-(2-0)-TYR 1.75 3OPZ[A,B,C,M] 1.70 3PJQ[A] β-D-Galp-(1-4)-α-D-Glcp 1.9
sialidase (TrSA)	3.2.1.18	Trypanosoma rangeli	AAC95493.1 AAC95494.1 AAC95495.1	O44049	1MZ5[A] 1.96 1MZ6[A] <carb_like_DAN> 2.75 1N1S[A] 2.02 1N1T[A] <carb_like_DAN> 1.81 1N1V[A] <carb_like_DAN> 2.00 1N1Y[A] α-D-Neup5Ac 2.00 1WCS[A] 2.21 2A75[A] α-L-Manp3F5Ac-(2-0)-TYR 1.55 2AGS[A] α-L-Manp3F-(2-0)-TYR 1.50 2FHR[A] α-L-Manp3F5Ac-(2-0)-TYR 2.40
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