Glycoside Hydrolase Family 33

Activities in Familysialidase or neuraminidase (EC 3.2.1.18); trans-sialidase (EC 2.4.1.-); 2-keto-3-deoxynononic acid hydrolase (EC 3.2.1.-); anhydrosialidase (EC 4.2.2.15); 3-deoxy-D-manno-octulosonic-acid hydrolase (EC 3.2.1.-)
Mechanism Retaining
ClanGH-E
3D Structure Status6-fold β-propeller
Catalytic Nucleophile/BaseTyr+Glu
Catalytic Proton DonorNot known
External resourcesHOMSTRAD;
Commercial Enzyme Provider(s)MEGAZYME;
Statistics GenBank accession (3547); Uniprot accession (686); PDB accession (144); 3D entries (19); cryst (0)
All (3289) Archaea (1) Bacteria (2885) Eukaryota (371) Viruses (32) Structure (19) Characterized (96)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 BACCAC_01090 (23295)   Bacteroides caccae ATCC 43185 ZP_01959484.1 A5ZDY0
4Q6K[A,B,C,D] 1.90
 sialidase (BtsA;BTSA;BT0455) 3.2.1.18 Bacteroides thetaiotaomicron VPI-5482 AAO75562.1
NP_809368.1
Q8AAK9
4BBW[A] 2.30
 sialidase (CpNanI;CPF_0721) 3.2.1.18 Clostridium perfringens ATCC 13124 ABG83208.1 Q0TT67
4L2E[A] 1.90
5TSP[A,B] 1.24
 exo-α-sialidase (NanI;CPSA;CPE0725) 3.2.1.18 Clostridium perfringens str. 13 BAB80431.1
NP_561641.1
Q8XMG4
2BF6[A] α-D-Neup5Ac
0.97
2VK5[A] 0.97
2VK6[A] <carb_like_DAN>
1.50
2VK7[A,B] α-L-Manp3F5Ac-(2-0)-TYR
1.2
 sialidase (NedA) 3.2.1.18 Micromonospora viridifaciens BAA00852.1
AAQ70871.1
Q02834
1EUR[A] 1.82
1EUS[A] <carb_like_DAN>
2.00
1EUT[A] 2.50
1EUU[A] β-D-Galp
2.50
1W8N[A] β-D-Galp
<carb_like_DAN>
2.1
1W8O[A] β-D-Galp-(1-4)-α-D-Glcp
1.70
1WCQ[A,B,C] <carb_like_DAN>
2.1
2BER[A] β-D-Neup5Ac
1.8
2BQ9[A,B,C] β-D-Galp
2.00
2BZD[A,B,C] β-D-Galp
2.0
4J9T[A] 1.40
 BDI_2946   Parabacteroides distasonis ATCC 8503 ABR44654.1 A6LG40
4FJ6[A] 1.90
 anhydrosialidase (RgNanH;HMPREF1201_01421) (intramolecular) 4.2.2.15 Ruminococcus gnavus ETD19277.1  
4X47[A] <carb_like_DAN>
2.00
4X49[A] <carb_like_G39>
2.01
4X4A[A] 1.71
4X6K[A] 1.94
 sialidase (NanH;STSA) 3.2.1.18 Salmonella typhimurium TA262 AAA27168.1 P29768
1DIL[A] D-1,2,3,4-deoxy-Xylp
<carb_like_AXP>
1.90
1DIM[A] 1.60
2SIL[A] 1.60
2SIM[A] <carb_like_DAN>
1.60
3SIL[A] 1.05
 sialidase A (NanA) 3.2.1.18 Streptococcus pneumoniae 6 CAA51473.1
ACJ76903.1
B8XTT3
P62575
Q59959
5KKY[A,B] 2.39
 neuraminidase (NanC) 3.2.1.18 Streptococcus pneumoniae G54 ACF56230.1 B5E561
4YW0[A,B] 2.05
4YW1[A,B] α-D-Neup5Ac
<carb_like_DAN>
2.25
4YW2[A,B] α-D-Neup5Ac-(2-6)-β-D-Galp
2.00
4YW3[A,B] α-D-Neup5Ac
<carb_like_DAN>
2.05
4YW4[A,B] 2.20
4YW5[A,B] <carb_like_G39>
2.30
4YZ1[A,B] 1.97
4YZ2[A,B] <carb_like_DAN>
2.06
4YZ3[A,B] <carb_like_G39>
2.38
4YZ4[A,B] α-D-Neup5Ac
2.15
4YZ5[A,B] α-D-Neup5Ac-(2-3)-β-D-Galp
<carb_like_DAN>
2.27
5F9T[A,B] α-L-Manp3F5Ac-(2-0)-TYR
2.05
 neuraminidase (NanC) 3.2.1.18 Streptococcus pneumoniae G54 ACF56230.1 B5E561
4YW0[A,B] 2.05
4YW1[A,B] α-D-Neup5Ac
<carb_like_DAN>
2.25
4YW2[A,B] α-D-Neup5Ac-(2-6)-β-D-Galp
2.00
4YW3[A,B] α-D-Neup5Ac
<carb_like_DAN>
2.05
4YW4[A,B] 2.20
4YW5[A,B] <carb_like_G39>
2.30
4YZ1[A,B] 1.97
4YZ2[A,B] <carb_like_DAN>
2.06
4YZ3[A,B] <carb_like_G39>
2.38
4YZ4[A,B] α-D-Neup5Ac
2.15
4YZ5[A,B] α-D-Neup5Ac-(2-3)-β-D-Galp
<carb_like_DAN>
2.27
5F9T[A,B] α-L-Manp3F5Ac-(2-0)-TYR
2.05
 sialidase A (NanA)   Streptococcus pneumoniae R6 AAL00340.1
ACJ76904.1
NP_359129.1
P62576
2VVZ[A,B] <carb_like_DAN>
2.5
2W20[A,B] 1.49
3H71[A,B] 1.70
3H72[A,B] α-D-Neup5Ac
1.70
3H73[A,B] <carb_like_DAN>
1.70
 intramolecular trans-sialidase B (NanB;SP_1687) 4.2.2.15 Streptococcus pneumoniae TIGR4 AAK75766.1
NP_346126.1
Q54727
2JKB[A] 1.54
2VW0[A] 2.3
2VW1[A] <carb_like_DAN>
2.39
2VW2[A] 1.7
4FOQ[A] 1.99
4FOV[A] 2.29
4FOW[A] 2.10
4FOY[A] 1.84
4FP2[A] 2.05
4FP3[A] 2.74
4FPC[A] 2.32
4FPE[A] 2.18
4FPF[A] 2.23
4FPG[A] 2.58
4FPH[A] 1.94
4FPJ[A] 1.98
4FPK[A] 2.40
4FPL[A] 2.10
4FPO[B] 2.59
4FPY[A] 2.18
4FQ4[A] 1.84
4XE9[A] 1.84
4XHB[A] 1.88
4XHX[A] 2.10
4XIK[A] 1.91
4XIL[A] 1.90
4XIO[A] 1.80
4XJ8[A] 2.75
4XJ9[A] 1.98
4XJA[A] 1.98
4XJU[A] 1.94
4XJW[A] 1.53
4XJZ[A] 1.56
4XMA[A] 2.09
4XMI[A] 1.97
4XOG[A] <carb_like_DAN>
2.09
4XYX[A] 2.10
 intramolecular trans-sialidase B (NanB;SP_1687) 4.2.2.15 Streptococcus pneumoniae TIGR4 AAK75766.1
NP_346126.1
Q54727
2JKB[A] 1.54
2VW0[A] 2.3
2VW1[A] <carb_like_DAN>
2.39
2VW2[A] 1.7
4FOQ[A] 1.99
4FOV[A] 2.29
4FOW[A] 2.10
4FOY[A] 1.84
4FP2[A] 2.05
4FP3[A] 2.74
4FPC[A] 2.32
4FPE[A] 2.18
4FPF[A] 2.23
4FPG[A] 2.58
4FPH[A] 1.94
4FPJ[A] 1.98
4FPK[A] 2.40
4FPL[A] 2.10
4FPO[B] 2.59
4FPY[A] 2.18
4FQ4[A] 1.84
4XE9[A] 1.84
4XHB[A] 1.88
4XHX[A] 2.10
4XIK[A] 1.91
4XIL[A] 1.90
4XIO[A] 1.80
4XJ8[A] 2.75
4XJ9[A] 1.98
4XJA[A] 1.98
4XJU[A] 1.94
4XJW[A] 1.53
4XJZ[A] 1.56
4XMA[A] 2.09
4XMI[A] 1.97
4XOG[A] <carb_like_DAN>
2.09
4XYX[A] 2.10
 neuraminidase (NanA;SpneT_02001817)   Streptococcus pneumoniae TIGR4 ZP_01407762.1  
2YA4[A,B] 1.80
2YA5[A,B] α-D-Neup5Ac
2.00
2YA6[A,B] <carb_like_DAN>
2.00
2YA7[A,B,C,D] <carb_like_ZMR>
1.89
2YA8[A,B] <carb_like_G39>
1.75
 sialidase / neuraminidase (NanH;VcNA;VCSA) 3.2.1.18 Vibrio cholerae 569B 395 AAA27546.1 P37060
1KIT[A] 2.30
1W0O[A] α-D-Neup5Ac
<carb_like_DAN>
1.9
1W0P[A] α-D-Neup5Ac
1.6
 sialidase / neuraminidase (NanH;VcNA;VCSA) 3.2.1.18 Vibrio cholerae 569B 395 AAA27546.1 P37060
1KIT[A] 2.30
1W0O[A] α-D-Neup5Ac
<carb_like_DAN>
1.9
1W0P[A] α-D-Neup5Ac
1.6
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 2-keto-3-deoxynononic acid sialidase (KDNase;AfKDNase;Afu4g13800;AFUA_4G13800) (6407) 3.2.1.18
3.2.1.-
Aspergillus fumigatus Af293 EAL89414.2  
2XCY[A,B] 1.84
2XZI[A,B] α-D-Neup
1.45
2XZJ[A,B] 1.84
2XZK[A,B] α-L-Manp3F-(2-0)-TYR
<carb_like_K99>
1.50
4M4N[A,B] 1.84
4M4U[A,B] 1.84
4M4V[A,B] <carb_like_DAN>
1.84
 sialidase (Neu2) (cytosolic) 3.2.1.18 Homo sapiens CAB41449.1
AAH69151.1
AAI07054.1
AAY24360.1
NP_005374.1
Q9Y3R4
1SNT[A] 1.75
1SO7[A] 1.49
1VCU[A,B] <carb_like_DAN>
2.85
2F0Z[A] <carb_like_ZMR>
2.80
2F10[A] <carb_like_BCZ>
2.90
2F11[A] β-D-4-deoxy-GlcpANAc-(1-1)-<non_carb>
2.57
2F12[A] β-D-4-deoxy-GlcpANAc-(1-1)-<non_carb>
2.27
2F13[A] α-L-4-deoxy-IdopANAc-(1-1)-<non_carb>
2.26
2F24[A] 1.76
2F25[A,B] <carb_like_DAN>
1.95
2F26[A] 1.58
2F27[A,B] <carb_like_DAN>
2.15
2F28[A] 1.67
2F29[A,B] <carb_like_DAN>
2.92
4NC5[A] α-L-Manp3F5Ac-(2-0)-TYR
2.51
4NCS[A] α-L-Glcp3F5Ac-(2-0)-TYR
2.20
 intramolecular trans-sialidase L (MDSA) 4.2.2.15 Macrobdella decora AAC47263.1 Q27701
1SLI[A] <carb_like_DAN>
2.00
1SLL[A] 2.00
2SLI[A] 1.80
3SLI[A] 1.80
4SLI[A] <carb_like_CNP>
1.80
 intramolecular trans-sialidase L (MDSA) 4.2.2.15 Macrobdella decora AAC47263.1 Q27701
1SLI[A] <carb_like_DAN>
2.00
1SLL[A] 2.00
2SLI[A] 1.80
3SLI[A] 1.80
4SLI[A] <carb_like_CNP>
1.80
 trans-sialidase (TcTS) 2.4.1.- Trypanosoma cruzi AAA66352.1
AAO30670.1
AAT21542.1
Q26966
1MR5[A] 2.25
1MS0[A,B] β-D-Galp-(1-4)-α-D-Glcp
<carb_like_DAN>
2.50
1MS1[A,B] <carb_like_DAN>
1.80
1MS3[A,B] 1.65
1MS4[A,B] 2.21
1MS5[A,B] 2.00
1MS8[A,B] <carb_like_DAN>
2.00
1MS9[A,B] β-D-Galp-(1-4)-β-D-Glcp
1.58
1S0I[A] α-D-Neup5Ac-(2-3)-β-D-Galp-(1-4)-α-D-Glcp
1.60
1S0J[A] α-D-Neup5Ac-(2-1)-<non_carb>
1.65
1S0K[A] α-L-Manp3F5Ac-(2-0)-TYR
1.60
2AH2[A] α-L-Manp3F5Ac-(2-0)-TYR
1.60
3B69[A] α-L-Manp3F5Ac9N-(2-0)-TYR
1.67
3OPZ[A,B,C,M] 3.40
3PJQ[A] β-D-Galp-(1-4)-α-D-Glcp
2.10
 sialidase (TrSA) 3.2.1.18 Trypanosoma rangeli AAC95493.1
AAC95494.1
AAC95495.1
O44049
1MZ5[A] 2.20
1MZ6[A] <carb_like_DAN>
2.90
1N1S[A] 1.64
1N1T[A] <carb_like_DAN>
1.60
1N1V[A] <carb_like_DAN>
2.10
1N1Y[A] α-D-Neup5Ac
2.80
2A75[A] α-L-Manp3F5Ac-(2-0)-TYR
1.95
2AGS[A] α-L-Manp3F-(2-0)-TYR
1.70
2FHR[A] α-L-Manp3F5Ac-(2-0)-TYR
2.20
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