Glycoside Hydrolase Family 31

Activities in Familyα-glucosidase (EC 3.2.1.20); α-galactosidase (EC 3.2.1.22); α-mannosidase (EC 3.2.1.24); α-1,3-glucosidase (EC 3.2.1.84); sucrase-isomaltase (EC 3.2.1.48) (EC 3.2.1.10); α-xylosidase (EC 3.2.1.177); α-glucan lyase (EC 4.2.2.13); isomaltosyltransferase (EC 2.4.1.-); oligosaccharide α-1,4-glucosyltransferase (EC 2.4.1.161); α-N-acetylgalactosaminidase (EC 3.2.1.49); sulfoquinovosidase (EC 3.2.1.199)
Mechanism Retaining
ClanGH-D
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorAsp
External resourcesCAZypedia; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME;
Statistics GenBank accession (16235); Uniprot accession (1017); PDB accession (122); 3D entries (28); cryst (0)
All (15727) Archaea (121) Bacteria (14063) Eukaryota (1530) unclassified (13) Structure (28) Characterized (123)
Archaea
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 α-glucosidase with α-mannosidase activity (MalA;SsMalA;Sso1_0793) 3.2.1.20
3.2.1.24
Saccharolobus solfataricus 98/2 AAC38215.1
ACX91047.1
AET42970.1
D0KQM8
G8GCW5
O59645
2G3M[A,B,C,D,E,F] 1.70
2G3N[A,B,C,D,E,F] β-D-Glcp-(1-1)-ethyl
2.20
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 SY94_3281   Agrobacterium tumefaciens F4 KJX86999.1  
5OHS[A,B,C,D,E,F,G,H] α-D-Glcp6SH-(1-1)-<non_carb>
2.30
5OHY[A,B,C,D] 1.71
 α-xylosidase (Yici_5;BoGH31A;BACOVA_02646;Bovatus_03066) 3.2.1.177 Bacteroides ovatus ATCC 8483 ALJ47674.1  
5JOU[A] 2.00
5JOV[A] α-L-Idop5F-(1-4)-ASP
2.00
 α-glucosidase (BT0339;BT_0339) 3.2.1.20 Bacteroides thetaiotaomicron VPI-5482 AAO75446.1
NP_809252.1
Q8AAX3
5F7C[A,B,C,D] 1.70
 α-glucosidase (BT3299;BT_3299) 3.2.1.20 Bacteroides thetaiotaomicron VPI-5482 AAO78405.1
NP_812211.1
Q8A2K6
5DJW[A,B] 3.00
 α-glucosidase / maltase-glucoamylase (Ro-aG1;RUMOBE_03919) 3.2.1.20 Blautia obeum ATCC 29174 ZP_01966167.1 A5ZY13
3FFJ[A,B] NMR
3M46[A,B] 2.09
3M6D[A,B] 2.10
3MKK[A,B] α-D-Glcp-(1-6)-β-D-Glcp
1.60
3N04[A,B] 1.60
3NQQ[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Manp
α-D-Glcp
2.00
3NSX[A,B] 1.90
3NUK[A,B] 2.00
3NXM[A,B,C,D] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.70
3PHA[A,B,C,D] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.12
3POC[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp
NMR
6C9X[A,B] 1.50
6C9Z[A,B] 2.15
6CA1[A,B] <carb_like_MIG>
1.70
6CA3[A,B] <carb_like_MIG>
1.60
 oligosaccharide α-1,4-transglucosylase 31B (CjAgd31B;CJA_3248) (Agd31B) 2.4.1.161 Cellvibrio japonicus Ueda107 ACE84782.1 B3PEE6
4B9Y[A] 1.80
4B9Z[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp-(1-4)-β-D-Gulp
1.00
4BA0[A] β-D-Glcp5F-(1-4)-ASP
1.95
5I23[A] 1.84
5I24[A] 1.90
5NPB[A] 1.35
5NPC[A] 1.70
5NPD[A] 1.70
5NPE[A] 1.7
 α-xylosidase 31A (CjXyl31A;CJA_2706) (Xyl31A) 3.2.1.177 Cellvibrio japonicus Ueda107 ACE86259.1 B3PBD9
2XVG[A] 0.98
2XVK[A] β-D-Xylp5F-(1-4)-ASP
2.40
2XVL[A] 1.22
 α-N-acetylgalactosaminidase (WOW_01747) 3.2.1.49 Enterococcus faecalis ATCC 10100 EOK08638.1  
6M76[A] 1.80
6M77[A] β-D-GalpNAc
1.73
 α-xylosidase (YicI;EcYicI;b3656) 3.2.1.177 Escherichia coli str. K-12 substr. MG1655 AAC76680.1
AAA62009.1
AIZ93543.1
ALI38655.1
AMC96544.1
AUG18368.1
AVI53824.1
AYG17401.1
NP_418113.1
P31434
1WE5[A,B,C,D,E,F] 1.70
1XSI[A,B,C,D,E,F] 1.4
1XSJ[A,B,C,D,E,F] 2.30
1XSK[A,B,C,D,E,F] β-D-Xylp5F-(1-4)-ASP
2.04
2F2H[A,B,C,D,E,F] α-D-Xylp-(1-6)-β-D-Glcp6SH-(1-1)-<non_carb>
2.11
 sulfoquinovosidase (SQase;YihQ;b3878) 3.2.1.199 Escherichia coli str. K-12 substr. MG1655 AAC76875.1
AAB03011.1
AIZ93343.1
ALI38454.1
AMC96746.1
AUG18567.1
AVI54027.1
AYG21326.1
NP_418314.1
P32138
5AED[A,B] 2.21
5AEE[A,B] α-D-Glcp6SH-(1-1)-<non_carb>
2.80
5AEG[A,B] α-L-Idop5F-(1-4)-ASP
1.18
5OHT[A,B] 2.35
 EUBREC_2592   [Eubacterium] rectale ATCC 33656 ACR76323.1 C4ZGB7
6PNR[A,C] 1.48
 dextranase/6-α-glucosyltransferase (Fjoh_4430) 3.2.1.11
2.4.1.-
Flavobacterium johnsoniae UW101 ABQ07434.1 A5FBI1
6JR6[A,B,C,D] 2.09
6JR7[A,B,C,D] α-D-Glcp-(1-6)-α-D-Glcp
2.80
6JR8[A,B,C,D] α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp
2.40
 cycloalternan-specific α-1,3-glucosidase / α-1,3-isomaltosidase (Kfla_1895) 3.2.1.204 Kribbella flavida DSM 17836 ADB30989.1 D2PPM7
5X3I[A,B] 3.10
5X3J[A,B] 1.46
5X3K[A,B] α-D-Glcp-(1-6)-α-D-Glcp
2.10
 cycloalternan-forming enzyme / 3-α-isomaltosyltransferase (CAFE;LmCAFE;Lmo2446) 2.4.1.- Listeria monocytogenes EGD-e CAD00524.1
NP_465969.1
Q8Y4J2
4KMQ[A] 2.00
4KWU[A] α-D-Glcp
β-D-Glcp
2.55
5F7U[A] α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-3)[α-D-Glcp-(1-6)]-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp
1.91
5HPO[A] α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.80
5HXM[A] α-D-Glcp-(1-6)-α-D-Glcp
1.76
5I0D[A,B] α-D-Glcp
α-D-Glcp-(1-3)-α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp
2.15
 6-α-glucosyltransferase (Ps6GT31A) (6GT31A) 2.4.1.- Paenibacillus sp. 598K BAV17026.1  
5X7O[A,B] 1.65
5X7P[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp
2.10
5X7Q[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N
α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp
2.18
5X7R[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N
α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-β-D-Glcp
β-D-Glcp
1.70
5X7S[A,B] NMR
 α-galactosidase (PsGal31A) (Gal31A) 3.2.1.22 Pseudopedobacter saltans NBRC 100064 BAR72452.1  
4XPO[A] 2.15
4XPP[A] β-D-Galp
2.0
4XPQ[A] β-L-Fucp
1.85
4XPR[A] 1.85
4XPS[A] α-D-Galp-(1-1)-<non_carb>
3.50
 cycloalternan-specific α-1,3-glucosidase (CADE;TpCADE;TpLmo0182;CQ11_05330) 3.2.1.204 Trueperella pyogenes TP6375 AHU89502.1  
5F7S[A,B] 1.80
5I0E[B] α-D-Glcp-(1-6)-α-D-Glcp
2.00
5I0F[B] α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-β-D-Glcp-(1-4)-ASP
2.25
5I0G[B] 1.90
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 glycosyl hydrolases family 31 family protein   Aspergillus niger    
6DRU[A] α-D-Xylp
α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp
β-D-Galp-(1-2)-α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp
1.50
6DRU[B] α-D-Xylp
α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp
β-D-Galp-(1-2)-α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp
1.50
 α-glucosidase (Agl;SBG) 3.2.1.20 Beta vulgaris BAM74081.1
BAM72724.1
 
3W37[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.89
3W38[A] 1.60
3WEL[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.60
3WEM[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.35
3WEN[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.59
3WEO[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
3.00
 α-1,3-glucosidase II α-subunit (GIIa;CTHT_0064960) 3.2.1.84 Chaetomium thermophilum var. thermophilum DSM 1495 EGS17181.1  
5DKX[A] 1.40
5DKY[A] <carb_like_NOJ>
2.30
5DKZ[A] α-D-Glcp-(1-3)-α-D-Glcp
1.38
5DL0[A] α-D-Glcp-(1-3)-α-D-Manp
1.78
5JQP[A] 1.30
 α-1,4-glucan lyase isozyme 1 (GLq1;Ag111) 4.2.2.13 Gracilariopsis lemaneiformis CAB51910.1
AAR66623.1
ABA16064.1
Q9STC1
2X2H[A,B,C,D] 1.60
2X2I[A,B,C,D] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
1.60
2X2J[A,B,C,D] <carb_like_NOJ>
2.80
4AMW[A,B,C,D] α-L-Idop5F-(1-4)-ASP
2.88
4AMX[A,B,C,D] β-D-Glcp5F-(1-4)-ASP
1.70
 α-glucosidase A (GAA) (lysosomal) 3.2.1.20 Homo sapiens CAA68763.1
AAA52506.1
AAB33842.1
AAH40431.1
ABI53718.1
ACM80396.1
ACM84029.1
BAH13042.1
CAA38907.1
CAA68764.1
CAC12967.1
CAE85448.1
NP_000143.1
P10253
P10253
Q8IWE7
5KZW[A] α-D-Glcp-(1-6)-β-D-Glcp
2.97
5KZX[A] α-D-Glcp-(1-6)-β-D-Glcp
1.94
5NN3[A] 1.75
5NN4[A] 1.45
5NN5[A] <carb_like_NOJ>
2.10
5NN6[A] <carb_like_MIG>
2.30
5NN8[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.05
 maltase-glucoamylase (MgaM;Mga;MGAML) 3.2.1.20 Homo sapiens AAC39568.2
AAI20873.1
AAL83560.1
NP_004659.1
O43451
Q8TE24
3TON[A,B] 1.49
3TOP[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.80
 maltase-glucoamylase (MgaM;Mga;MGAML) 3.2.1.20 Homo sapiens AAC39568.2
AAI20873.1
AAL83560.1
AAP21875.1
NP_004659.1
O43451
Q86UM5
Q8TE24
2QLY[A] 2.01
2QMJ[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.29
3CTT[A] <carb_like_3CU>
1.57
3L4T[A] <carb_like_BJ1>
1.06
3L4U[A] <carb_like_DSK>
2.03
3L4V[A] <carb_like_KTL>
1.90
3L4W[A] <carb_like_MIG>
1.70
3L4X[A] <carb_like_NR3>
1.61
3L4Y[A] <carb_like_NR4>
1.95
3L4Z[A] <carb_like_SSD>
2.05
 sucrase-isomaltase (SI) 3.2.1.10
3.2.1.48
Homo sapiens AAT18166.1
AAA60551.1
AAI15035.1
AAI16453.1
AAI16454.1
AAI32835.1
AAI32861.1
AAT19036.1
ABH87017.1
ACM85129.1
ACP58672.1
CAA45140.1
NP_001032.1
P14410
3LPO[A,B,C,D] α-D-Manp-(1-6)[α-D-Manp-(1-3)]-β-D-Manp-(1-4)-β-D-GlcpNAc-(1-4)-D-1-deoxy-GlcpNAc
3.20
3LPP[A,B,C,D] <carb_like_KTL>
2.34
 α-glucosidase II (α-GluII;AB;GanAB;GANAB;G2an;Kiaa0088 )   Mus musculus AAC53182.1
AAH94437.1
AAI17889.1
AAI17890.1
AAI28070.1
BAB30982.1
BAC27099.1
BAC38370.1
BAC65483.1
BAE29025.1
NP_032086.1
A1A4T2
Q149A6
Q8BHN3
Q8BHN3
5F0E[A] 1.70
5H9O[A,C] α-D-Glcp
2.66
5HJO[A,C] D-1,2-deoxy-Glcp
α-D-2-deoxy-Glcp-(1-3)-D-1,2-deoxy-Glcp
2.20
5HJR[A,C] β-D-Glcp5F-(1-4)-ASP
1.99
5IED[A] <carb_like_CTS>
1.86
5IEE[A] <carb_like_NOJ>
1.55
5IEF[A] <carb_like_NBV>
2.18
5IEG[A] 1.95
unclassified
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 α-xylosidase (Mexyl31)   uncultured microorganism BAU88419.1  
5ZN6[A,B,C,D,E,F,G,H] 1.85
5ZN7[A,B,C,D,E,F,G,H] α-D-Xylp
1.60
Top


Last update: 2021-01-11 © Copyright 1998-2021
AFMB - CNRS - Université d'Aix-Marseille