Activities in Family	α-glucosidase (EC 3.2.1.20); α-galactosidase (EC 3.2.1.22); α-mannosidase (EC 3.2.1.24); α-1,3-glucosidase (EC 3.2.1.84); sucrase-isomaltase (EC 3.2.1.48) (EC 3.2.1.10); α-xylosidase (EC 3.2.1.177); α-glucan lyase (EC 4.2.2.13); isomaltosyltransferase (EC 2.4.1.-); oligosaccharide α-1,4-glucosyltransferase (EC 2.4.1.161); α-N-acetylgalactosaminidase (EC 3.2.1.49); sulfoquinovosidase (EC 3.2.1.199)
Mechanism	Retaining
Clan	GH-D
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Asp
External resources	CAZypedia; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME;
Statistics	GenBank accession (16235); Uniprot accession (1017); PDB accession (122); 3D entries (28); cryst (0)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
α-glucosidase with α-mannosidase activity (MalA;SsMalA;Sso1_0793)	3.2.1.20 3.2.1.24	Saccharolobus solfataricus 98/2	AAC38215.1 ACX91047.1 AET42970.1	D0KQM8 G8GCW5 O59645	2G3M[A,B,C,D,E,F] 1.70 2G3N[A,B,C,D,E,F] β-D-Glcp-(1-1)-ethyl 2.20
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
SY94_3281		Agrobacterium tumefaciens F4	KJX86999.1		5OHS[A,B,C,D,E,F,G,H] α-D-Glcp6SH-(1-1)-<non_carb> 2.30 5OHY[A,B,C,D] 1.71
α-xylosidase (Yici_5;BoGH31A;BACOVA_02646;Bovatus_03066)	3.2.1.177	Bacteroides ovatus ATCC 8483	ALJ47674.1		5JOU[A] 2.00 5JOV[A] α-L-Idop5F-(1-4)-ASP 2.00
α-glucosidase (BT0339;BT_0339)	3.2.1.20	Bacteroides thetaiotaomicron VPI-5482	AAO75446.1 NP_809252.1	Q8AAX3	5F7C[A,B,C,D] 1.70
α-glucosidase (BT3299;BT_3299)	3.2.1.20	Bacteroides thetaiotaomicron VPI-5482	AAO78405.1 NP_812211.1	Q8A2K6	5DJW[A,B] 3.00
α-glucosidase / maltase-glucoamylase (Ro-aG1;RUMOBE_03919)	3.2.1.20	Blautia obeum ATCC 29174	ZP_01966167.1	A5ZY13	3FFJ[A,B] NMR 3M46[A,B] 2.09 3M6D[A,B] 2.10 3MKK[A,B] α-D-Glcp-(1-6)-β-D-Glcp 1.60 3N04[A,B] 1.60 3NQQ[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Manp α-D-Glcp 2.00 3NSX[A,B] 1.90 3NUK[A,B] 2.00 3NXM[A,B,C,D] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.70 3PHA[A,B,C,D] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.12 3POC[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp NMR 6C9X[A,B] 1.50 6C9Z[A,B] 2.15 6CA1[A,B] <carb_like_MIG> 1.70 6CA3[A,B] <carb_like_MIG> 1.60
oligosaccharide α-1,4-transglucosylase 31B (CjAgd31B;CJA_3248) (Agd31B)	2.4.1.161	Cellvibrio japonicus Ueda107	ACE84782.1	B3PEE6	4B9Y[A] 1.80 4B9Z[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp-(1-4)-β-D-Gulp 1.00 4BA0[A] β-D-Glcp5F-(1-4)-ASP 1.95 5I23[A] 1.84 5I24[A] 1.90 5NPB[A] 1.35 5NPC[A] 1.70 5NPD[A] 1.70 5NPE[A] 1.7
α-xylosidase 31A (CjXyl31A;CJA_2706) (Xyl31A)	3.2.1.177	Cellvibrio japonicus Ueda107	ACE86259.1	B3PBD9	2XVG[A] 0.98 2XVK[A] β-D-Xylp5F-(1-4)-ASP 2.40 2XVL[A] 1.22
α-N-acetylgalactosaminidase (WOW_01747)	3.2.1.49	Enterococcus faecalis ATCC 10100	EOK08638.1		6M76[A] 1.80 6M77[A] β-D-GalpNAc 1.73
α-xylosidase (YicI;EcYicI;b3656)	3.2.1.177	Escherichia coli str. K-12 substr. MG1655	AAC76680.1 AAA62009.1 AIZ93543.1 ALI38655.1 AMC96544.1 AUG18368.1 AVI53824.1 AYG17401.1 NP_418113.1	P31434	1WE5[A,B,C,D,E,F] 1.70 1XSI[A,B,C,D,E,F] 1.4 1XSJ[A,B,C,D,E,F] 2.30 1XSK[A,B,C,D,E,F] β-D-Xylp5F-(1-4)-ASP 2.04 2F2H[A,B,C,D,E,F] α-D-Xylp-(1-6)-β-D-Glcp6SH-(1-1)-<non_carb> 2.11
sulfoquinovosidase (SQase;YihQ;b3878)	3.2.1.199	Escherichia coli str. K-12 substr. MG1655	AAC76875.1 AAB03011.1 AIZ93343.1 ALI38454.1 AMC96746.1 AUG18567.1 AVI54027.1 AYG21326.1 NP_418314.1	P32138	5AED[A,B] 2.21 5AEE[A,B] α-D-Glcp6SH-(1-1)-<non_carb> 2.80 5AEG[A,B] α-L-Idop5F-(1-4)-ASP 1.18 5OHT[A,B] 2.35
EUBREC_2592		[Eubacterium] rectale ATCC 33656	ACR76323.1	C4ZGB7	6PNR[A,C] 1.48
dextranase/6-α-glucosyltransferase (Fjoh_4430)	3.2.1.11 2.4.1.-	Flavobacterium johnsoniae UW101	ABQ07434.1	A5FBI1	6JR6[A,B,C,D] 2.09 6JR7[A,B,C,D] α-D-Glcp-(1-6)-α-D-Glcp 2.80 6JR8[A,B,C,D] α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp 2.40
cycloalternan-specific α-1,3-glucosidase / α-1,3-isomaltosidase (Kfla_1895)	3.2.1.204	Kribbella flavida DSM 17836	ADB30989.1	D2PPM7	5X3I[A,B] 3.10 5X3J[A,B] 1.46 5X3K[A,B] α-D-Glcp-(1-6)-α-D-Glcp 2.10
cycloalternan-forming enzyme / 3-α-isomaltosyltransferase (CAFE;LmCAFE;Lmo2446)	2.4.1.-	Listeria monocytogenes EGD-e	CAD00524.1 NP_465969.1	Q8Y4J2	4KMQ[A] 2.00 4KWU[A] α-D-Glcp β-D-Glcp 2.55 5F7U[A] α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp-(1-3)[α-D-Glcp-(1-6)]-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp 1.91 5HPO[A] α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.80 5HXM[A] α-D-Glcp-(1-6)-α-D-Glcp 1.76 5I0D[A,B] α-D-Glcp α-D-Glcp-(1-3)-α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp 2.15
6-α-glucosyltransferase (Ps6GT31A) (6GT31A)	2.4.1.-	Paenibacillus sp. 598K	BAV17026.1		5X7O[A,B] 1.65 5X7P[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp 2.10 5X7Q[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp 2.18 5X7R[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-β-D-Glcp β-D-Glcp 1.70 5X7S[A,B] NMR
α-galactosidase (PsGal31A) (Gal31A)	3.2.1.22	Pseudopedobacter saltans NBRC 100064	BAR72452.1		4XPO[A] 2.15 4XPP[A] β-D-Galp 2.0 4XPQ[A] β-L-Fucp 1.85 4XPR[A] 1.85 4XPS[A] α-D-Galp-(1-1)-<non_carb> 3.50
cycloalternan-specific α-1,3-glucosidase (CADE;TpCADE;TpLmo0182;CQ11_05330)	3.2.1.204	Trueperella pyogenes TP6375	AHU89502.1		5F7S[A,B] 1.80 5I0E[B] α-D-Glcp-(1-6)-α-D-Glcp 2.00 5I0F[B] α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-6)-β-D-Glcp-(1-4)-ASP 2.25 5I0G[B] 1.90
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
glycosyl hydrolases family 31 family protein		Aspergillus niger			6DRU[A] α-D-Xylp α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp β-D-Galp-(1-2)-α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp 1.50 6DRU[B] α-D-Xylp α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp β-D-Galp-(1-2)-α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp 1.50
α-glucosidase (Agl;SBG)	3.2.1.20	Beta vulgaris	BAM74081.1 BAM72724.1		3W37[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.89 3W38[A] 1.60 3WEL[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.60 3WEM[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.35 3WEN[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.59 3WEO[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 3.00
α-1,3-glucosidase II α-subunit (GIIa;CTHT_0064960)	3.2.1.84	Chaetomium thermophilum var. thermophilum DSM 1495	EGS17181.1		5DKX[A] 1.40 5DKY[A] <carb_like_NOJ> 2.30 5DKZ[A] α-D-Glcp-(1-3)-α-D-Glcp 1.38 5DL0[A] α-D-Glcp-(1-3)-α-D-Manp 1.78 5JQP[A] 1.30
α-1,4-glucan lyase isozyme 1 (GLq1;Ag111)	4.2.2.13	Gracilariopsis lemaneiformis	CAB51910.1 AAR66623.1 ABA16064.1	Q9STC1	2X2H[A,B,C,D] 1.60 2X2I[A,B,C,D] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp 1.60 2X2J[A,B,C,D] <carb_like_NOJ> 2.80 4AMW[A,B,C,D] α-L-Idop5F-(1-4)-ASP 2.88 4AMX[A,B,C,D] β-D-Glcp5F-(1-4)-ASP 1.70
α-glucosidase A (GAA) (lysosomal)	3.2.1.20	Homo sapiens	CAA68763.1 AAA52506.1 AAB33842.1 AAH40431.1 ABI53718.1 ACM80396.1 ACM84029.1 BAH13042.1 CAA38907.1 CAA68764.1 CAC12967.1 CAE85448.1 NP_000143.1	P10253 P10253 Q8IWE7	5KZW[A] α-D-Glcp-(1-6)-β-D-Glcp 2.97 5KZX[A] α-D-Glcp-(1-6)-β-D-Glcp 1.94 5NN3[A] 1.75 5NN4[A] 1.45 5NN5[A] <carb_like_NOJ> 2.10 5NN6[A] <carb_like_MIG> 2.30 5NN8[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.05
maltase-glucoamylase (MgaM;Mga;MGAML)	3.2.1.20	Homo sapiens	AAC39568.2 AAI20873.1 AAL83560.1 NP_004659.1	O43451 Q8TE24	3TON[A,B] 1.49 3TOP[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.80
maltase-glucoamylase (MgaM;Mga;MGAML)	3.2.1.20	Homo sapiens	AAC39568.2 AAI20873.1 AAL83560.1 AAP21875.1 NP_004659.1	O43451 Q86UM5 Q8TE24	2QLY[A] 2.01 2QMJ[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.29 3CTT[A] <carb_like_3CU> 1.57 3L4T[A] <carb_like_BJ1> 1.06 3L4U[A] <carb_like_DSK> 2.03 3L4V[A] <carb_like_KTL> 1.90 3L4W[A] <carb_like_MIG> 1.70 3L4X[A] <carb_like_NR3> 1.61 3L4Y[A] <carb_like_NR4> 1.95 3L4Z[A] <carb_like_SSD> 2.05
sucrase-isomaltase (SI)	3.2.1.10 3.2.1.48	Homo sapiens	AAT18166.1 AAA60551.1 AAI15035.1 AAI16453.1 AAI16454.1 AAI32835.1 AAI32861.1 AAT19036.1 ABH87017.1 ACM85129.1 ACP58672.1 CAA45140.1 NP_001032.1	P14410	3LPO[A,B,C,D] α-D-Manp-(1-6)[α-D-Manp-(1-3)]-β-D-Manp-(1-4)-β-D-GlcpNAc-(1-4)-D-1-deoxy-GlcpNAc 3.20 3LPP[A,B,C,D] <carb_like_KTL> 2.34
α-glucosidase II (α-GluII;AB;GanAB;GANAB;G2an;Kiaa0088 )		Mus musculus	AAC53182.1 AAH94437.1 AAI17889.1 AAI17890.1 AAI28070.1 BAB30982.1 BAC27099.1 BAC38370.1 BAC65483.1 BAE29025.1 NP_032086.1	A1A4T2 Q149A6 Q8BHN3 Q8BHN3	5F0E[A] 1.70 5H9O[A,C] α-D-Glcp 2.66 5HJO[A,C] D-1,2-deoxy-Glcp α-D-2-deoxy-Glcp-(1-3)-D-1,2-deoxy-Glcp 2.20 5HJR[A,C] β-D-Glcp5F-(1-4)-ASP 1.99 5IED[A] <carb_like_CTS> 1.86 5IEE[A] <carb_like_NOJ> 1.55 5IEF[A] <carb_like_NBV> 2.18 5IEG[A] 1.95
unclassified
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
α-xylosidase (Mexyl31)		uncultured microorganism	BAU88419.1		5ZN6[A,B,C,D,E,F,G,H] 1.85 5ZN7[A,B,C,D,E,F,G,H] α-D-Xylp 1.60
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