Activities in Family	β-galactosidase (EC 3.2.1.23) ; β-mannosidase (EC 3.2.1.25); β-glucuronidase (EC 3.2.1.31); α-L-arabinofuranosidase (EC 3.2.1.55); mannosylglycoprotein endo-β-mannosidase (EC 3.2.1.152); exo-β-glucosaminidase (EC 3.2.1.165); α-L-arabinopyranosidase (EC 3.2.1.-); β-galacturonidase (EC 3.2.1.-); β-xylosidase (EC 3.2.1.37); β-D-galactofuranosidase (EC 3.2.1.146); β-glucosidase (EC 3.2.1.21)
Mechanism	Retaining
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
External resources	CAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (24396); Uniprot accession (1654); PDB accession (207); 3D entries (53); cryst (2)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
exo-β-glucosaminidase / exochitosanase (CsxA)	3.2.1.165	Amycolatopsis orientalis	AAX62629.2 AAX62629.1	Q56F26	2VZO[A,B] 1.90 2VZS[A,B] β-D-GlcpN 2.20 2VZT[A,B] β-D-GlcpN-(1-1)-<non_carb> 2.20 2VZU[A,B] β-D-GlcpN-(1-1)-<non_carb> 2.20 2VZV[A,B] β-D-GlcpN-(1-4)-β-D-GlcpN 1.55 2X05[A,B] <carb_like_X05> 1.65 2X09[A,B] <carb_like_X09> 1.50
β-D-galactosidase	3.2.1.23	Arthrobacter sp. 32cB	AHY00656.1		6ETZ[A] 2.00 6H1P[A] 2.30 6SE8[A] 1.60 6SE9[A] β-D-Fruf β-D-Galp β-D-Galp-(1-4)-β-D-Glcp 3.00 6SEA[A] β-D-Galp β-D-Galp-(1-4)-β-D-Glcp 1.45 6SEB[A] β-D-Galp1S-(1-1)-<non_carb> 2.05 6SEC[A] β-D-Galp-(1-1)-<non_carb> 2.40 6SED[A] β-D-Galp 1.90 6ZJP[A] NMR 6ZJQ[A] β-D-Galp 2.50 6ZJR[A] 2.0 6ZJS[A] β-D-Galp 2.00 6ZJT[A] β-D-Galp β-D-Galp-(1-4)-β-D-Fruf 1.55 6ZJU[A] α-D-Glcp-(1-2)-β-D-Fruf 2.40 6ZJV[A] 1.49 6ZJW[A] β-D-Galp 1.77 6ZJX[A] 1.80
β-galactosidase (LacZ)	3.2.1.23	Arthrobacter sp. C2-2	CAD29775.1	Q8KRF6	1YQ2[A,B,C,D,E,F] 2.30
β-galactosidase A / B / C / D / 91 (Bga;BgaD;β-Gal-A;β-Gal-B;β-Gal-C;β-Gal-D;β-Glα-91)	3.2.1.23	Bacillus circulans ATCC 31382	BAJ61032.1		4YPJ[A,B] 1.70
β-galactosidase	3.2.1.23	Bacillus circulans ATCC 31382	QJU69416.1		7CWD[A] α-D-Galp-(1-4)-GLU α-D-Glcp 1.25 7CWI[A] 3.26
BACCELL_00063		Bacteroides cellulosilyticus DSM 14838	EEF92278.1		6B6L[A,B] 1.95
β-glucuronidase (BSEG_01974)	3.2.1.31	Bacteroides dorei 5_1_36/D4	EEO45833.1		6ED1[A,B,C,D] 1.50
BF0029		Bacteroides fragilis NCTC 9343	CAH05808.1	Q5LJ68	3FN9[A,B,C,D] 1.70
β-glucuronidase (BF0328)	3.2.1.31	Bacteroides fragilis NCTC 9343	CAH06099.1	Q5LIC7	3CMG[A] 2.00 5Z1A[A] 2.00
β-glucuronidase (BMSP;HMPREF2532_04191)	3.2.1.31	Bacteroides ovatus KLE1656	KXT42440.1		6D8K[B,C,D] 2.09 6D8K[A] 2.09
β-mannosidase (BT0458;BT_0458) (Man2A)	3.2.1.25	Bacteroides thetaiotaomicron VPI-5482	AAO75565.1 NP_809371.1	Q8AAK6	2JE8[A,B] 2.05 2VJX[A,B] <carb_like_IFL> 1.96 2VL4[A,B] <carb_like_MNM> 3.20 2VMF[A,B] <carb_like_MVL> 1.76 2VO5[A,B] 2.20 2VOT[A,B] 2.31 2VQT[A,B] <carb_like_15A> 1.26 2VQU[A,B] <carb_like_NOY> 2.10 2VR4[A,B] <carb_like_17B> 0.97 2WBK[A,B] β-D-Manp2F-(1-1)-<non_carb> NMR
BT3179		Bacteroides thetaiotaomicron VPI-5482	AAO78285.1 NP_812091.1	Q8A2X6	3DEC[A] 2.19
BT1626		Bacteroides thetaiotaomicron VPI-5482	AAO76733.1 NP_810539.1	Q8A799	3BGA[A,B] 2.35
(BuGH2A)		Bacteroides uniformis NP1			5T98[B] 1.93 5T99[A,B] 1.40 5T98[A] 1.93
Agarolytic β-galactosidase (BuGH2C)	3.2.1.23	Bacteroides uniformis NP1			5T9A[B,C,D] 1.93 5T9G[A,B,C,D] 1.90 5T9A[A] 1.93
β-glucuronidase (BuGUS-2; M094_3283)	3.2.1.31	Bacteroides uniformis str. 3978 T3 i	KDS62590.1		5UJ6[A,B] 1.85 6D50[A,B] β-D-GlcpA 2.57 6D8G[A,B] 1.88 6NZG[A,B] 2.06
β-glucuronidase (BuGUS-1; ERS417307_01040; Uida_4)	3.2.1.31	Bacteroides uniformis str. 3978 T3 i	CUO13985.1		6D1N[A,B] 2.00 6D41[A,B] α-D-GlcpA β-D-GlcpA 2.10 6D6W[A,B,C,D] α-D-GlcpA 1.47 6D7F[A,B,C,D,E,F] β-D-GlcpA-(1-1)-<non_carb> 2.00 6D89[A,B,C,D] 1.70
β-glucuronidase (BuGUS-3; M094_3281)		Bacteroides uniformis str. 3978 T3 i	KDS64778.1		6D1P[A,B] 3.00
BVU_0391		Bacteroides vulgatus ATCC 8482	ABR38109.1	A6KXE5	3GM8[A] NMR
β-galactosidase (BIF3)	3.2.1.23	Bifidobacterium bifidum DSM 20215	CAC14566.2 AAP64961.1 ABI07182.1 CAD19366.1	Q9F4D5	6QUB[A,B] β-D-Galp 2.30 6QUC[A,B] 1.98 6QUD[A] α-D-2-deoxy-Galp-(1-4)-GLU 1.05
lacto-N-neotetraose β-galactosidase (BbgIII)	3.2.1.23	Bifidobacterium bifidum JCM 1254	BAI94821.1		5DMY[A,B,C] 1.80
HMPREF0168_2111 (UidA)	3.2.1.31	Bifidobacterium dentium ATCC 27679	WP_003843201.1	E0QAF3	5Z1B[A,B,C,D] β-D-GlcpA-(1-1)-<non_carb> β-D-GlcpA-(1-2)-unknown 1.80
β-glucuronidase (BglR;CPE0147)	3.2.1.31	Clostridium perfringens str. 13	BAB79853.1 CAD12654.1 NP_561063.1	Q8VNV4 Q8XP19	4JKM[A,B] 2.25 6CXS[A,B] 2.15
DICTH_1429	3.2.1.31	Dictyoglomus thermophilum H-6-12	ACI18585.1	B5YFE0	6XXW[A] 1.96
β-mannosidase (DtMan;DICTH_1692)	3.2.1.25	Dictyoglomus thermophilum H-6-12	ACI19676.1	B5YAN4	5N6U[A,B,C,D] D-1-deoxy-Manp 1.80
β-galacturonidase (EtGalAse)	3.2.1.-	Eisenbergiella tayi			6NCW[B,C,D] 1.20 6NCX[A,B,C,D] α-D-GalpA 1.70 6NCW[A] 1.20
RG69_19155 (LacZ)		Escherichia coli S40	APL67431.1		6TSH[A,B,C,D] <carb_like_DGJ> 1.84 6TSK[A,B,C,D] β-L-Ribp 1.70 6TTE[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.40
β-galactosidase Z (LacZ;Lac-Z;b0344;EO53_16565)	3.2.1.23	Escherichia coli str. K-12 substr. MG1655	AAA24053.1 AAB18068.1 AAC73447.1 AAW06767.1 AAY73171.1 ABN72582.1 AEL16982.1 AIZ92504.1 ALI41840.1 AMC97588.1 AUG15134.1 AVI54877.1 CAA23573.1 CAC87491.1 NP_414878.1	B8LFD6 P00722 Q8VNN2	1BGL[A] 1.4 1BGM[I] 1.60 1DP0[A] 2.40 1F49[A] 1.60 1F4A[A] 1.75 1F4H[A] 2.37 1GHO[I] 2.00 1HN1[A] 3.50 1JYN[A] β-D-Galp-(1-4)-β-D-Glcp 1.80 1JYV[A] β-D-Galp-(1-1)-<non_carb> 2.60 1JYW[A] β-D-Galp-(1-1)-<non_carb> NMR 1JYX[A] β-D-Galp1S-(1-1)-<non_carb> 1.75 1JYY[A] β-D-Galp2F-(1-4)-β-D-Glcp 2.00 1JYZ[I] β-D-Galp2F-(1-4)-β-D-Glcp 1.80 1JZ0[A] α-D-Galp2F-(1-4)-GLU 1.95 1JZ1[I] α-D-Galp2F-(1-4)-GLU 2.19 1JZ2[A] α-D-Galp2F-(1-4)-GLU 1.80 1JZ3[A] α-D-2-deoxy-Galp-(1-4)-GLU 1.95 1JZ4[A] α-D-2-deoxy-Galp-(1-4)-GLU 1.62 1JZ5[A] α-D-Galp β-D-Galp 1.70 1JZ6[A] <carb_like_GTZ> 1.47 1JZ7[A] β-D-Galp 2.20 1JZ8[A] β-D-Galp-(1-6)-β-D-Glcp β-D-Galp-(1-6)-β-D-Manp 1.10 1PX3[A,B,C,D] 2.60 1PX4[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 1.90 3CZJ[A,B,C,D] β-D-Galp 1.87 3DYM[A,B,C,D] 2.40 3DYO[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 1.85 3DYP[A] 1.20 3E1F[1,2,3,4] 1.50 3I3B[A,B,C,D] α-D-Galp β-D-Galp 2.05 3I3D[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.85 3I3E[A,B,C,D] 2.35 3IAP[A,B,C,D] 2.00 3IAQ[A,B,C,D] 1.65 3J7H[A,B,C,D] 2.00 3MUY[1,2,3,4] 1.76 3MUZ[1,2,3,4] 2.8 3MV0[1,2,3,4] 2.25 3MV1[1,2,3,4] 2.20 3SEP[A,B,C,D] 2.30 3T08[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.00 3T09[A,B,C,D] α-D-Galp β-D-Galp 2.00 3T0A[A,B,C,D] 1.70 3T0B[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.40 3T0D[A,B,C,D] α-D-Galp β-D-Galp 3.00 3T2O[A,B,C,D] NMR 3T2P[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.06 3T2Q[A,B,C,D] α-D-Galp β-D-Galp 2.40 3VD3[A,B,C,D] 2.00 3VD4[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 1.80 3VD5[A,B,C,D] 1.13 3VD7[A,B,C,D] <carb_like_GTZ> 2.60 3VD9[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 1.07 3VDA[A,B,C,D] 2.00 3VDB[A,B,C,D] α-D-Galp β-D-Galp 1.90 3VDC[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.00 4CKD[A,B,C,D] 2.00 4DUV[A,B,C,D] α-D-2-deoxy-Galp-(1-4)-GLU 1.70 4DUW[A,B,C,D] β-D-Galp-(1-6)-β-D-Glcp 2.00 4DUX[A,B,C,D] β-L-Ribp 1.51 4TTG[A,B,C,D] 2.40 5A1A[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 1.60 6CVM[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 1.90 6DRV[A,B,C,D] 1.70 6KUZ[A,B,C,D] β-D-Galp-(1-1)-<non_carb> 1.38 6X1Q[A,B,C,D] 2.05
β-glucuronidase (UidA;GusA;GurA;b1617)	3.2.1.31	Escherichia coli str. K-12 substr. MG1655	AAC74689.1 AAA68923.1 AAB30197.1 AAR75623.1 ABE20937.1 ACR88323.1 AIZ91278.1 ALI40610.1 AMC98822.1 AUG16387.1 AVI56132.1 AYG19328.1 CAJ55495.1 CAJ55496.1 CAJ55497.1 CAJ55498.1 CAJ55499.1 CAJ55500.1 NP_416134.1	P05804	3K46[A,B] 1.70 3K4A[A,B] 3.26 3K4D[A,B] <carb_like_EVA> 1.85 3LPF[A,B] 1.75 3LPG[A,B] 2.30 4JHZ[A,B] 1.19 5CZK[A,B] 2.20
β-glucuronidase (EUBELI_20590)	3.2.1.31	[Eubacterium] eligens ATCC 27750	ACR73734.1	C4Z6Z2	6BJQ[A] β-D-GlcpA NMR 6BJW[A] NMR 6BO6[A] 2.35 6D4O[A] 1.80
β-glucuronidase (CG447_12395)	3.2.1.31	Faecalibacterium prausnitzii A2165	ATP00638.1 EEU97254.1		6ED2[A] 2.03
C4Q21_10165	3.2.1.31	Faecalibacterium prausnitzii APC918/95b	AXB29257.1		6U7I[A,B,C,D] 2.05
β-glucuronidase (FP2_04120)	3.2.1.31	Faecalibacterium prausnitzii L2-6 L2/6	CBK98066.1	D4K3H3	6MVF[A,B,C,D,E,F] 1.60
β-1,2-glucuronidase (BN863_22060)	3.2.1.31	Formosa agariphila KMM 3901	CDF79918.1		6HPD[A] 1.79
FsGUS (β-galacturonidase/β-glucuronidase)	3.2.1.31 3.2.1.-	Fusicatenibacter saccharivorans			6NCY[B] 2.02 6NCZ[A,B,C,D,E,F] β-D-GlcpA-(1-1)-<non_carb> 1.15 6NCY[A] 2.02
β-glucuronidase (HMPREF0539_0313; UidA)	3.2.1.31	Lactobacillus rhamnosus LMS2-1	EEN81625.1		6ECA[A,B] 1.93
β-glucuronidase (ZP_02032394.1;PARMER_02407) (26901)	3.2.1.31	Parabacteroides merdae ATCC 43184	ZP_02032394.1		6D7J[A,B,C,D] 2.59 6DXU[A,B,C,D] 2.32
β-galactosidase (BgaL; ParbDG)	3.2.1.23	Paracoccus sp. 32d	ACY69080.1	D1LZK0	5EUV[A,B] 1.80 5LDR[A,B] α-D-Glcp β-D-Galp 1.14
β-glucuronidase	3.2.1.31	Roseburia hominis			6MVH[B,C,D] 1.90 6MVH[A] 1.90
β-glucuronidase	3.2.1.31	Ruminococcus gnavus			6MVG[B,C] 2.00 6MVG[A] 2.00
β-glucuronidase (UidA)	3.2.1.31	Ruminococcus gnavus E1	AAQ76046.1	Q6W7J7	5Z18[A,B,C,D,E,F] 2.36 5Z19[A,B,C,D,E,F] 1.90 6EC6[A,B] 2.50 6JZ1[A,B] 1.96 6JZ2[A,B] 2.30 6JZ3[A,B] 2.49 6JZ4[A,B] <carb_like_EVA> 2.47 6JZ5[A,B] β-D-GlcpA 2.63 6JZ6[A,B] 1.80 6JZ7[A,B] 1.90 6JZ8[A,B] β-D-GlcpA 2.23
β-glucuronidase (SAG0698)	3.2.1.31	Streptococcus agalactiae 2603V/R	AAM99585.1 NP_687713.1 Q8E0N2		4JKK[A] 2.30 4JKL[A,B] 1.76
β-1,4-galactosidase A (BgaA;SP0648;SP_0648)	3.2.1.23	Streptococcus pneumoniae TIGR4	AAK74795.1 ABA35731.1 NP_345155.1	Q9KGU0	4CU6[A] 2.51 4CU7[A] 1.45 4CU8[A] <carb_like_DGJ> 1.06 4CUC[A] β-D-Galp-(1-4)-β-D-GlcpNAc 1.45
β-galactosidase Z (LacZ;TmLac;TM1193)	3.2.1.23	Thermotoga maritima MSB8	AAD36268.1 AAA50597.1 AGL50124.1 AHD18900.1 AKE28970.1 CAA04513.1 NP_228998.1	G4FEF3 Q56307	6S6Z[A,B,C,D,E,F,G,H] 1.80 6SD0[A,B,C,D] 2.80
Betα-glucuronidase (uidA_2)	3.2.1.31	uncultured Clostridium sp.			6U7J[A,B,C,D] 1.80
exo-β-mannanase (XacMan2A; XAC3075) (XacMan2A)	3.2.1.25	Xanthomonas citri pv. citri str. 306	AAM37920.1 NP_643384.1	Q8PI23	6BYC[A] 2.80 6BYE[A,B] β-D-Manp 1.80 6BYG[A,B] β-D-Manp 1.84 6BYI[A,B] β-D-Manp 2.04
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
β-mannosidase (MndA;AnigerBM2;An11g06540)	3.2.1.25	Aspergillus niger CBS 513.88	CAK96951.1 XP_001394632.2 XP_001394632.2	A2QWU9	cryst
β-glucuronidase (PGUS;GUS)	3.2.1.31	Aspergillus oryzae Li-3	ABU68712.1	A7XS03	5C70[A,B] 2.10 5C71[A,B,C,D] α-D-GlcpA-(1-1)-<non_carb> 1.75
β-glucuronidase b (GusB;GUSB)	3.2.1.31	Homo sapiens	AAA52561.1 AAH14142.1 AAQ96851.1 AAR75622.1 ABH92596.1 ACH09936.1 ACM86199.1 BAD97126.1 BAG35619.1 BAG61991.1 BAG64768.1 CAC41170.1 NP_000172.1	P08236	1BHG[A,B] 1.62 3HN3[A,B,D,E] β-D-Manp 1.60
β-galactosidase (Lac4;BgaL)	3.2.1.23	Kluyveromyces lactis CBS2359	AAA35265.1	P00723	3OB8[A,B,C,D] β-D-Galp 1.80 3OBA[A,B,C,D] 1.47
β-mannosidase	3.2.1.25	Mus musculus	AAK18177.1 AAH31409.1 BAE24954.1 BAE38715.1 BAE41690.1 NP_081564.1	Q8K2I4 Q99MS1	6DDT[A] 1.54 6DDU[A] β-D-Manp 1.98
β-mannosidase (ThMan2A) (Man2A)		Trichoderma harzianum IOC-3844	AID21733.1		4CVU[A] 1.90 4UOJ[A,B] 2.10
exo-β-glucosaminidase (Gls93)	3.2.1.165	Trichoderma reesei PC-3-7	BAD99604.1	Q4R1C4	cryst
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