Glycoside Hydrolase Family 2

Activities in Familyβ-galactosidase (EC 3.2.1.23) ; β-mannosidase (EC 3.2.1.25); β-glucuronidase (EC 3.2.1.31); α-L-arabinofuranosidase (EC 3.2.1.55); mannosylglycoprotein endo-β-mannosidase (EC 3.2.1.152); exo-β-glucosaminidase (EC 3.2.1.165)
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (11711); Uniprot accession (1662); PDB accession (120); 3D entries (28); cryst (2)
All (10460) Archaea (46) Bacteria (10105) Eukaryota (290) unclassified (19) Structure (28 - 2 cryst) Characterized (147)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 exo-β-glucosaminidase / exochitosanase (CsxA) 3.2.1.165 Amycolatopsis orientalis AAX62629.2
AAX62629.1
Q56F26
2VZO[A,B] 2.24
2VZS[A,B] β-D-GlcpN
1.85
2VZT[A,B] β-D-GlcpN-(1-1)-<non_carb>
2.20
2VZU[A,B] β-D-GlcpN-(1-1)-<non_carb>
2.10
2VZV[A,B] β-D-GlcpN-(1-4)-β-D-GlcpN
2.70
2X05[A,B] <carb_like_X05>
2.30
2X09[A,B] <carb_like_X09>
2.40
 β-galactosidase (LacZ) 3.2.1.23 Arthrobacter sp. C2-2 CAD29775.1 Q8KRF6
1YQ2[A,B,C,D,E,F] 1.90
 β-galactosidase A / B / C / D / 91 (Bga;BgaD;β-Gal-A;β-Gal-B;β-Gal-C;β-Gal-D;β-Glα-91) 3.2.1.23 Bacillus circulans ATCC 31382 BAJ61032.1  
4YPJ[A,B] 2.50
 BACCELL_00063   Bacteroides cellulosilyticus DSM 14838 EEF92278.1  
6B6L[A,B] 2.00
 BF0029   Bacteroides fragilis NCTC 9343 CAH05808.1 Q5LJ68
3FN9[A,B,C,D] 2.70
 BF0328   Bacteroides fragilis NCTC 9343 CAH06099.1 Q5LIC7
3CMG[A] 1.90
 β-mannosidase (BT0458;BT_0458) (Man2A) 3.2.1.25 Bacteroides thetaiotaomicron VPI-5482 AAO75565.1
NP_809371.1
Q8AAK6
2JE8[A,B] 1.7
2VJX[A,B] <carb_like_IFL>
1.85
2VL4[A,B] <carb_like_MNM>
1.90
2VMF[A,B] <carb_like_MVL>
2.1
2VO5[A,B] 2.3
2VOT[A,B] 1.95
2VQT[A,B] <carb_like_15A>
2.1
2VQU[A,B] <carb_like_NOY>
1.9
2VR4[A,B] <carb_like_17B>
1.8
2WBK[A,B] β-D-Manp2F-(1-1)-<non_carb>
2.10
 BT3179   Bacteroides thetaiotaomicron VPI-5482 AAO78285.1
NP_812091.1
Q8A2X6
3DEC[A] 2.80
 BT1626   Bacteroides thetaiotaomicron VPI-5482 AAO76733.1
NP_810539.1
Q8A799
3BGA[A,B] 2.10
 BuGH2A   Bacteroides uniformis NP1    
5T98[B] 2.60
5T99[A,B] 2.40
5T98[A] 2.60
 BuGH2C   Bacteroides uniformis NP1    
5T9A[B,C,D] 2.50
5T9G[A,B,C,D] 2.40
5T9A[A] 2.50
 β-glucuronidase   Bacteroides uniformis str. 3978 T3 i KDS62590.1  
5UJ6[A,B] 1.90
 BVU_0391   Bacteroides vulgatus ATCC 8482 ABR38109.1 A6KXE5
3GM8[A] 2.40
 lacto-N-neotetraose β-galactosidase (BbgIII) 3.2.1.23 Bifidobacterium bifidum JCM 1254 BAI94821.1  
5DMY[A,B,C] 1.95
 β-glucuronidase (BglR;CPE0147)   Clostridium perfringens str. 13 BAB79853.1
CAD12654.1
NP_561063.1
Q8VNV4
Q8XP19
4JKM[A,B] 2.26
 β-mannosidase (DtMan;DICTH_1692) 3.2.1.25 Dictyoglomus thermophilum H-6-12 ACI19676.1 B5YAN4
5N6U[A,B,C,D] D-1-deoxy-Manp
3.08
 β-galactosidase (LacZ) 3.2.1.23 Escherichia coli CAC87491.1 Q8VNN2
4TTG[A,B,C,D] 1.60
 β-galactosidase Z (LacZ;Lac-Z;b0344;EO53_16565) 3.2.1.23 Escherichia coli str. K-12 substr. MG1655 AAA24053.1
AAB18068.1
AAC73447.1
AAW06767.1
AAY73171.1
ABN72582.1
AEL16982.1
AIZ92504.1
ALI41840.1
AMC97588.1
CAA23573.1
NP_414878.1
B8LFD6
P00722
1BGL[A] 2.50
1BGM[I] 2.50
1DP0[A] 1.70
1F49[A] 2.50
1F4A[A] 2.80
1F4H[A] 2.80
1GHO[I] 2.50
1HN1[A] 3.00
1JYN[A] β-D-Galp-(1-4)-β-D-Glcp
1.80
1JYV[A] β-D-Galp-(1-1)-<non_carb>
1.75
1JYW[A] β-D-Galp-(1-1)-<non_carb>
1.55
1JYX[A] β-D-Galp1S-(1-1)-<non_carb>
1.75
1JYY[A] β-D-Galp2F-(1-4)-β-D-Glcp
2.70
1JYZ[I] β-D-Galp2F-(1-4)-β-D-Glcp
2.70
1JZ0[A] α-D-Galp2F-(1-4)-GLU
2.60
1JZ1[I] α-D-Galp2F-(1-4)-GLU
2.60
1JZ2[A] α-D-Galp2F-(1-4)-GLU
2.10
1JZ3[A] α-D-2-deoxy-Galp-(1-4)-GLU
1.75
1JZ4[A] α-D-2-deoxy-Galp-(1-4)-GLU
2.10
1JZ5[A] α-D-Galp
β-D-Galp
1.80
1JZ6[A] <carb_like_GTZ>
2.10
1JZ7[A] β-D-Galp
1.50
1JZ8[A] β-D-Galp-(1-6)-β-D-Glcp
β-D-Galp-(1-6)-β-D-Manp
1.50
1PX3[A,B,C,D] 1.60
1PX4[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb>
1.60
3CZJ[A,B,C,D] β-D-Galp
2.05
3DYM[A,B,C,D] 2.05
3DYO[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb>
1.80
3E1F[1,2,3,4] 3.00
3I3B[A,B,C,D] α-D-Galp
β-D-Galp
2.20
3I3D[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb>
2.20
3I3E[A,B,C,D] 2.10
3IAP[A,B,C,D] 2.00
3IAQ[A,B,C,D] 2.70
3J7H[A,B,C,D] 3.20
3MUY[1,2,3,4] 2.50
3MUZ[1,2,3,4] 1.90
3MV0[1,2,3,4] 2.20
3MV1[1,2,3,4] 2.20
3SEP[A,B,C,D] 2.05
3T08[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb>
2.00
3T09[A,B,C,D] α-D-Galp
β-D-Galp
1.75
3T0A[A,B,C,D] 1.90
3T0B[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb>
2.40
3T0D[A,B,C,D] α-D-Galp
β-D-Galp
1.93
3T2O[A,B,C,D] 1.85
3T2P[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb>
2.60
3T2Q[A,B,C,D] α-D-Galp
β-D-Galp
2.40
3VD3[A,B,C,D] 2.80
3VD4[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb>
2.00
3VD5[A,B,C,D] 2.70
3VD7[A,B,C,D] <carb_like_GTZ>
2.87
3VD9[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb>
2.05
3VDA[A,B,C,D] 2.50
3VDB[A,B,C,D] α-D-Galp
β-D-Galp
2.05
3VDC[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb>
2.55
4CKD[A,B,C,D] 13.00
4DUV[A,B,C,D] α-D-2-deoxy-Galp-(1-4)-GLU
2.10
4DUW[A,B,C,D] β-D-Galp-(1-6)-β-D-Glcp
2.20
4DUX[A,B,C,D] β-L-Ribp
2.30
5A1A[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb>
2.20
 β-glucuronidase (UidA;GusA;GurA;b1617) 3.2.1.31 Escherichia coli str. K-12 substr. MG1655 AAC74689.1
AAA68923.1
AAB30197.1
AAR75623.1
ABE20937.1
ACR88323.1
AIZ91278.1
ALI40610.1
AMC98822.1
CAJ55495.1
CAJ55496.1
CAJ55497.1
CAJ55498.1
CAJ55499.1
CAJ55500.1
NP_416134.1
P05804
3K46[A,B] 2.50
3K4A[A,B] 2.90
3K4D[A,B] <carb_like_EVA>
2.39
3LPF[A,B] 2.26
3LPG[A,B] 2.42
4JHZ[A,B] 2.83
5CZK[A,B] 2.39
 β-galactosidase (BgaL;(Par?DG) 3.2.1.23 Paracoccus sp. 32d ACY69080.1 D1LZK0
5EUV[A,B] 2.40
5LDR[A,B] α-D-Glcp
β-D-Galp
3.15
 SAG0698   Streptococcus agalactiae 2603V/R AAM99585.1
NP_687713.1
 
4JKK[A] 2.59
4JKL[A,B] 2.29
 β-1,4-galactosidase A (BgaA;SP0648;SP_0648) 3.2.1.23 Streptococcus pneumoniae TIGR4 AAK74795.1
ABA35731.1
NP_345155.1
Q9KGU0
4CU6[A] 2.70
4CU7[A] 2.20
4CU8[A] <carb_like_DGJ>
2.50
4CUC[A] β-D-Galp-(1-4)-β-D-GlcpNAc
2.20
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 β-mannosidase (MndA;AnigerBM2;An11g06540) 3.2.1.25 Aspergillus niger CBS 513.88 CAK96951.1
XP_001394632.2
XP_001394632.2
A2QWU9
cryst    
 β-glucuronidase (PGUS;GUS) 3.2.1.31 Aspergillus oryzae Li-3 ABU68712.1 A7XS03
5C70[A,B] 3.10
5C71[A,B,C,D] α-D-GlcpA-(1-1)-<non_carb>
2.62
 β-glucuronidase b (GusB;GUSB) 3.2.1.31 Homo sapiens AAA52561.1
AAH14142.1
AAQ96851.1
AAR75622.1
ABH92596.1
ACH09936.1
ACM86199.1
BAD97126.1
BAG35619.1
BAG61991.1
BAG64768.1
CAC41170.1
NP_000172.1
P08236
1BHG[A,B] 2.53
3HN3[A,B,D,E] β-D-Manp
1.70
 β-galactosidase (Lac4;BgaL) 3.2.1.23 Kluyveromyces lactis CBS2359 AAA35265.1 P00723
3OB8[A,B,C,D] β-D-Galp
2.80
3OBA[A,B,C,D] 2.75
 β-mannosidase (ThMan2A) (Man2A)   Trichoderma harzianum IOC-3844 AID21733.1  
4CVU[A] 1.90
4UOJ[A,B] 2.50
 exo-β-glucosaminidase (Gls93) 3.2.1.165 Trichoderma reesei PC-3-7 BAD99604.1 Q4R1C4
cryst    
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