Glycoside Hydrolase Family 27

Known Activities	α-galactosidase (EC 3.2.1.22); α-N-acetylgalactosaminidase (EC 3.2.1.49); isomalto-dextranase (EC 3.2.1.94); β-L-arabinopyranosidase (EC 3.2.1.88)
Mechanism	Retaining
Clan	GH-D
3D Structure Status	( β / α ) ₈
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Asp
Note	Mechanistic commonality with family GH36 demonstrated by Comfort et al. (2007) Biochemistry 46:3319-3330 (PMID: 17323919).
External resources	CAZypedia; InterPro; PFAM; PRINTS; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (634); Uniprot accession (304); PDB accession (33); 3D entries (10); cryst (1)

Summary

All (459) Archaea (1) Bacteria (229) Eukaryota (228) unclassified (1) Structure (10 - 1 cryst) Characterized (66)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
isomaltodextranase (G2d;Imd)	3.2.1.94	Arthrobacter globiformis T6	BAA06424.1 BAC76886.2	Q44052 Q7WSN5	cryst
BH1870		Bacillus halodurans C-125	BAB05589.1 NP_242736.1	Q9KBQ5	3CC1[A,B]
β-L-arabinopyranosidase (AgaA2;SaArap27A;SAV_2186) (Arap27A)	3.2.1.88	Streptomyces avermitilis MA-4680	BAC69897.1 NP_823362.1		3A21[A,B] 3A22[A,B] 3A23[A,B]
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
α-N-acetylgalactosaminidase	3.2.1.49	Gallus gallus	AAA16614.1 AAE69554.1	Q90744	1KTB[A] 1KTC[A]
α-galactosidase A (Gla;α-GAL)	3.2.1.22	Homo sapiens	AAA51676.1 AAA52514.1 AAA52559.1 AAB64203.1 AAE66433.1 AAE69556.1 AAH02689.1 AAN92813.1 AAP35510.1 AAQ31818.1 AAQ68716.1 AAQ81221.1 AAY03282.1 AAY71885.1 AAY71886.1 AAY71887.1 AAY71888.1 AAY71889.1 AAY71890.1 AAY71891.1 AAY71892.1 AAY71893.1 AAY71894.1 AAY72531.1 AAY72532.1 AAY72533.1 AAY72534.1 AAY72535.1 AAY72536.1 AAY72537.1 AAY72538.1 AAY72539.1 AAY72540.1 ABE23230.1 ABL16360.1 ABL21591.1 ACH14962.1 ACH14962.1 ACK10792.1 BAA34059.1 BAD96347.1 BAF83784.1 BAG59647.1 CAA29232.1 CAA32617.1 CAB55878.1 CAC09605.1 CAE85447.1 CAV33302.1 NP_000160.1	P06280.1 Q53Y83	1R46[A,B] 1R47[A,B] 3GXN[A,B] 3GXP[A,B] 3GXT[A,B] 3HG2[A,B] 3HG3[A,B] 3HG4[A,B] 3HG5[A,B] 3LX9[A,B] 3LXA[A,B] 3LXB[A,B] 3LXC[A,B] 3S5Y[A,B] 3S5Z[A,B] 3TV8[A,B]
α-N-acetylgalactosaminidase / α-galactosidase B (bK250D10.5;NagA;α-NAGAL)	3.2.1.22 3.2.1.49	Homo sapiens	AAA36351.1 AAA51677.1 AAA59902.1 AAB06718.1 AAE69555.1 AAH00095.1 ACM80412.1 ACM85401.1 ACM85401.1 CAB41237.1 CAG30413.1 CAX14869.1 CAX14870.1 NP_000253.1	P17050.2	3H53[A,B] 3H54[A,B] 3H55[A,B] 3IGU[A,B]
α-galactosidase (Agl1)	3.2.1.22	Hypocrea jecorina		Q92456	1SZN[A] 1T0O[A]
α-galactosidase I (Os10g0493600)	3.2.1.22	Oryza sativa Japonica Group	AAM92832.1 AAO85428.1 AAP54412.1 ABB47819.1 BAB12570.1 BAF26853.1 NP_922125.1	Q9FXT4 Q9FXT4.1	1UAS[A]
α-galactosidase 1 (Mel1)	3.2.1.22	Saccharomyces cerevisiae	AAE69557.1 CAA26888.1 CBY84983.1 CBY84984.1	P04824.1	3LRK[A] 3LRL[A] 3LRM[A,B,C,D]
α-galactosidase I (GalI;UvGalI)	3.2.1.22	Umbelopsis vinacea			3A5V[A]