Glycoside Hydrolase Family 27

Activities in Familyα-galactosidase (EC 3.2.1.22); α-N-acetylgalactosaminidase (EC 3.2.1.49); isomalto-dextranase (EC 3.2.1.94); β-L-arabinopyranosidase (EC 3.2.1.88); galactan:galactan galactosyltransferase (EC 2.4.1.-)
Mechanism Retaining
ClanGH-D
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorAsp
NoteMechanistic commonality with family GH36 demonstrated by Comfort et al. (2007) Biochemistry 46:3319-3330 (PMID: 17323919).
External resourcesCAZypedia; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (1394); Uniprot accession (327); PDB accession (46); 3D entries (13); cryst (0)
All (1138) Archaea (1) Bacteria (807) Eukaryota (329) unclassified (1) Structure (13) Characterized (66)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 isomalto-dextranase (G2d;Imd) 3.2.1.94 Arthrobacter globiformis T6 BAC76886.2
BAA06424.1
Q44052
Q7WSN5
5AWO[A] 1.44
5AWP[A] α-D-Glcp-(1-6)-β-D-Glcp
2.00
5AWQ[A] α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp
1.48
 BH1870   Bacillus halodurans C-125 BAB05589.1
NP_242736.1
Q9KBQ5
3CC1[A,B] 2.00
 BF1418   Bacteroides fragilis NCTC 9343 CAH07128.1 Q5LFG6
4NZJ[A] 1.57
 BF4189 / BF9343_4081   Bacteroides fragilis NCTC 9343 CAH09862.1 Q5L7T7
4OGZ[A,B] 2.00
 β-L-arabinopyranosidase (Abp;HUS_CDS23) 3.2.1.88 Geobacillus stearothermophilus T-6 NCIMB 40222    
4NX0[A,B,C,E,F,G,H] 2.28
4NXK[A,B,C,D,E,F,G,H] 2.30
4NZF[A,B,C,D,E,F,G,H] β-L-Arap
2.19
4NX0[D] 2.28
 β-L-arabinopyranosidase (AgaA2;SaArap27A;SAV_2186) (Arap27A) 3.2.1.88 Streptomyces avermitilis MA-4680 = NBRC 14893 BAC69897.1
NP_823362.1
 
3A21[A,B] 1.51
3A22[A,B] α-L-Arap
1.90
3A23[A,B] β-D-Galp
1.90
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 α-N-acetylgalactosaminidase (α-NAGAL) 3.2.1.49 Gallus gallus AAA16614.1
AAE69554.1
Q90744
1KTB[A] 1.90
1KTC[A] α-D-GalpNAc
2.40
 α-galactosidase A (Gla;α-GAL) 3.2.1.22 Homo sapiens AAH02689.1
AAA51676.1
AAA52514.1
AAA52559.1
AAB64203.1
AAE66433.1
AAE69556.1
AAN92813.1
AAP35510.1
AAQ31818.1
AAQ68716.1
AAQ81221.1
AAY03282.1
AAY71885.1
AAY71886.1
AAY71887.1
AAY71888.1
AAY71889.1
AAY71890.1
AAY71891.1
AAY71892.1
AAY71893.1
AAY71894.1
AAY72531.1
AAY72532.1
AAY72533.1
AAY72534.1
AAY72535.1
AAY72536.1
AAY72537.1
AAY72538.1
AAY72539.1
AAY72540.1
ABE23230.1
ABL16360.1
ABL21591.1
ACH14962.1
ACK10792.1
BAA34059.1
BAD96347.1
BAF83784.1
BAG59647.1
BAT62474.1
CAA29232.1
CAA32617.1
CAB55878.1
CAC09605.1
CAE85447.1
CAV33302.1
NP_000160.1
P06280
Q53Y83
1R46[A,B] 3.25
1R47[A,B] α-D-Galp
3.45
3GXN[A,B] 3.01
3GXP[A,B] D-1-deoxy-GlcpNAc
D-1-deoxy-Manp
α-D-Galp
2.20
3GXT[A,B] D-1-deoxy-GlcpNAc
β-D-Manp-(1-3)-D-1-deoxy-Manp
<carb_like_NOJ>
2.70
3HG2[A,B] β-D-Galp
2.30
3HG3[A,B] α-D-Galp-(1-6)-α-D-Glcp
1.90
3HG4[A,B] β-D-Glcp
β-D-Talp2F-(1-4)-ASP
2.30
3HG5[A,B] α-D-Galp
β-D-Galp
2.30
3LX9[A,B] α-D-GalpNAc
2.04
3LXA[A,B] α-D-Galp
3.04
3LXB[A,B] 2.85
3LXC[A,B] 2.35
3S5Y[A,B] <carb_like_DGJ>
2.10
3S5Z[A,B] α-D-Galp
β-D-Galp
2.00
3TV8[A,B] <carb_like_DGJ>
2.64
4NXS[A,B] <carb_like_2OZ>
2.55
 α-N-acetylgalactosaminidase / α-galactosidase B (bK250D10.5;NagA;α-NAGAL) 3.2.1.22
3.2.1.49
Homo sapiens AAA36351.1
AAA51677.1
AAA59902.1
AAB06718.1
AAE69555.1
AAH00095.1
ACM80412.1
ACM85401.1
ASU96594.1
CAB41237.1
CAG30413.1
CAX14869.1
CAX14870.1
NP_000253.1
P17050
3H53[A,B] 2.01
3H54[A,B] α-D-GalpNAc
2.20
3H55[A,B] α-D-Galp
1.91
3IGU[A,B] β-D-Talp2F-(1-4)-ASP
2.15
4DO4[A,B] <carb_like_DJN>
1.40
4DO5[A,B] <carb_like_DGJ>
1.51
4DO6[A,B] α-D-Glcp
β-D-Glcp
1.60
 α-galactosidase I (Os10g0493600) 3.2.1.22 Oryza sativa Japonica Group BAB12570.1
AAM92832.1
AAO85428.1
AAP54412.1
ABB47819.1
BAF26853.1
BAT11449.1
BAT11450.1
BAT11451.1
BAT11452.1
NP_922125.1
F4MGX5
Q9FXT4
Q9FXT4
1UAS[A] α-D-Galp
1.50
 α-galactosidase 1 (Mel1;ScMel1) 3.2.1.22 Saccharomyces cerevisiae CAA26888.1
AAE69557.1
CBY84983.1
CBY84984.1
CUW00903.1
CUW00905.1
CUW00906.1
F4N9N8
P04824
3LRK[A] 1.95
3LRL[A] α-D-Galp-(1-6)-β-D-Glcp
2.40
3LRM[A,B,C,D] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf
2.70
 α-galactosidase (Agl1) 3.2.1.22 Trichoderma reesei   Q92456
1T0O[A] β-D-Galp
1.96
1SZN[A] 1.54
 α-galactosidase I (GalI;UvGalI) 3.2.1.22 Umbelopsis vinacea    
3A5V[A] 2.00
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