Glycoside Hydrolase Family 27

Activities in Familyα-galactosidase (EC 3.2.1.22); α-N-acetylgalactosaminidase (EC 3.2.1.49); isomalto-dextranase (EC 3.2.1.94); β-L-arabinopyranosidase (EC 3.2.1.88); galactan:galactan galactosyltransferase (EC 2.4.1.-)
Mechanism Retaining
ClanGH-D
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorAsp
NoteMechanistic commonality with family GH36 demonstrated by Comfort et al. (2007) Biochemistry 46:3319-3330 (PMID: 17323919).
External resourcesCAZypedia; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (3309); Uniprot accession (93); PDB accession (51); 3D entries (14); cryst (0)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 BH1870   Alkalihalobacillus halodurans C-125 BAB05589.1
NP_242736.1
 
3CC1[A,B] 2.00
 isomalto-dextranase (G2d;Imd) 3.2.1.94 Arthrobacter globiformis T6 BAC76886.2
BAA06424.1
Q44052
Q7WSN5
5AWO[A] 1.44
5AWP[A] α-D-Glcp-(1-6)-β-D-Glcp
2.00
5AWQ[A] α-D-Glcp-(1-6)-α-D-Glcp
α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp
1.48
 BF1418   Bacteroides fragilis NCTC 9343 CAH07128.1  
4NZJ[A] 1.57
 BF4189 / BF9343_4081   Bacteroides fragilis NCTC 9343 CAH09862.1  
4OGZ[A,B] 2.00
 β-L-arabinopyranosidase (Abp;HUS_CDS23) 3.2.1.88 Geobacillus stearothermophilus T-6 NCIMB 40222    
4NX0[A,B,C,E,F,G,H] 2.28
4NXK[A,B,C,D,E,F,G,H] 2.30
4NZF[A,B,C,D,E,F,G,H] β-L-Arap
2.19
4NX0[D] 2.28
 β-L-arabinopyranosidase (AgaA2;SaArap27A;SAV_2186) (Arap27A) 3.2.1.88 Streptomyces avermitilis MA-4680 = NBRC 14893 BAC69897.1
NP_823362.1
Q82L26
3A21[A,B] 1.51
3A22[A,B] α-L-Arap
1.90
3A23[A,B] β-D-Galp
1.90
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 α-N-acetylgalactosaminidase (α-NAGAL) 3.2.1.49 Gallus gallus AAA16614.1
AAE69554.1
Q90744
1KTB[A] 1.90
1KTC[A] α-D-GalpNAc
2.40
 α-galactosidase A (Gla;α-GAL) 3.2.1.22 Homo sapiens AAH02689.1
AAA51676.1
AAA52514.1
AAA52559.1
AAB64203.1
AAE66433.1
AAE69556.1
AAN92813.1
AAP35510.1
AAQ31818.1
AAQ68716.1
AAQ81221.1
AAY03282.1
AAY71885.1
AAY71886.1
AAY71887.1
AAY71888.1
AAY71889.1
AAY71890.1
AAY71891.1
AAY71892.1
AAY71893.1
AAY71894.1
AAY72531.1
AAY72532.1
AAY72533.1
AAY72534.1
AAY72535.1
AAY72536.1
AAY72537.1
AAY72538.1
AAY72539.1
AAY72540.1
ABE23230.1
ABL16360.1
ABL21591.1
ACH14962.1
ACK10792.1
BAA34059.1
BAD96347.1
BAF83784.1
BAG59647.1
BAT62474.1
CAA29232.1
CAA32617.1
CAB55878.1
CAC09605.1
CAE85447.1
CAV33302.1
NP_000160.1
A0A0S3Q2A7
B4DLT5
P06280
Q53HF3
Q53Y83
1R46[A,B] 3.25
1R47[A,B] α-D-Galp
3.45
3GXN[A,B] 3.01
3GXP[A,B] D-1-deoxy-GlcpNAc
D-1-deoxy-Manp
α-D-Galp
2.20
3GXT[A,B] D-1-deoxy-GlcpNAc
β-D-Manp-(1-3)-D-1-deoxy-Manp
<carb_like_NOJ>
2.70
3HG2[A,B] β-D-Galp
2.30
3HG3[A,B] α-D-Galp-(1-6)-α-D-Glcp
1.90
3HG4[A,B] β-D-Glcp
β-D-Talp2F-(1-4)-ASP
2.30
3HG5[A,B] α-D-Galp
β-D-Galp
2.30
3LX9[A,B] α-D-GalpNAc
2.04
3LXA[A,B] α-D-Galp
3.04
3LXB[A,B] 2.85
3LXC[A,B] 2.35
3S5Y[A,B] <carb_like_DGJ>
2.10
3S5Z[A,B] α-D-Galp
β-D-Galp
2.00
3TV8[A,B] <carb_like_DGJ>
2.64
4NXS[A,B] <carb_like_2OZ>
2.55
6IBK[A,B] 1.99
6IBM[A,B] 2.07
6IBR[A,B] 2.02
6IBT[A,B] 2.04
 α-N-acetylgalactosaminidase / α-galactosidase B (bK250D10.5;NagA;α-NAGAL) 3.2.1.22
3.2.1.49
Homo sapiens AAA36351.1
AAA51677.1
AAA59902.1
AAB06718.1
AAE69555.1
AAH00095.1
ACM80412.1
ACM85401.1
ASU96594.1
CAB41237.1
CAG30413.1
CAX14869.1
CAX14870.1
NP_000253.1
A0A024R1Q5
A0A248RGB1
P17050
3H53[A,B] 2.01
3H54[A,B] α-D-GalpNAc
2.20
3H55[A,B] α-D-Galp
1.91
3IGU[A,B] β-D-Talp2F-(1-4)-ASP
2.15
4DO4[A,B] <carb_like_DJN>
1.40
4DO5[A,B] <carb_like_DGJ>
1.51
4DO6[A,B] α-D-Glcp
β-D-Glcp
1.60
 α-galactosidase 3.2.1.22 Nicotiana benthamiana   A0A384E148
6F4C[B] 2.80
 α-galactosidase I (Os10g0493600) 3.2.1.22 Oryza sativa Japonica Group BAB12570.1
AAM92832.1
AAO85428.1
AAP54412.1
ABB47819.1
BAF26853.1
BAT11449.1
BAT11450.1
BAT11451.1
BAT11452.1
NP_922125.1
A0A0N7KS02
A0A0P0XVT5
A0A0P0XW07
F4MGX5
Q9FXT4
Q9FXT4
1UAS[A] α-D-Galp
1.50
 α-galactosidase 1 (Mel1;ScMel1) 3.2.1.22 Saccharomyces cerevisiae CAA26888.1
AAE69557.1
CBY84983.1
CBY84984.1
CUW00903.1
CUW00905.1
CUW00906.1
F4N9N8
P04824
3LRK[A] 1.95
3LRL[A] α-D-Galp-(1-6)-β-D-Glcp
2.40
3LRM[A,B,C,D] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf
2.70
 α-galactosidase (Agl1) 3.2.1.22 Trichoderma reesei   Q92456
1T0O[A] β-D-Galp
1.96
1SZN[A] 1.54
 α-galactosidase I (GalI;UvGalI) 3.2.1.22 Umbelopsis vinacea    
3A5V[A] 2.00
Top


Last update: 2021-11-30 © Copyright 1998-2021
AFMB - CNRS - Université d'Aix-Marseille