Activities in Family	Galactan:galactan α-galactosyltransferase (EC 2.4.1.-); Galactomannan exo-α-1,6-galactosidase (EC 3.2.1.-); α-galactosidase (EC 3.2.1.22); α-N-acetylgalactosaminidase (EC 3.2.1.49); [retaining] β-L-arabinopyranosidase / β-L-arabinosidase (EC 3.2.1.88); [retaining] isomalto-dextranase (EC 3.2.1.94);
Mechanism	Retaining a
Clan	GH-D
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Asp
Note	Mechanistic commonality with family GH36 demonstrated by Comfort et al. (2007) Biochemistry 46:3319-3330 (PMID: 17323919).
External resources	CAZypedia; PRINTS; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (4924); Uniprot accession (92); PDB accession (51); 3D entries (14); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
isomalto-dextranase (G2d;Imd)	3.2.1.94	Arthrobacter globiformis T6	BAC76886.2 BAA06424.1	Q44052 Q7WSN5	5AWO[A] 1.44 5AWP[A] α-D-Glcp-(1-6)-β-D-Glcp 2.00 5AWQ[A] α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp 1.48
BF1418		Bacteroides fragilis NCTC 9343	CAH07128.1		4NZJ[A] 1.57
BF4189 / BF9343_4081		Bacteroides fragilis NCTC 9343	CAH09862.1		4OGZ[A,B] 2.00
β-L-arabinopyranosidase (Abp;HUS_CDS23)	3.2.1.88	Geobacillus stearothermophilus T-6 NCIMB 40222			4NX0[A,B,C,E,F,G,H] 2.28 4NXK[A,B,C,D,E,F,G,H] 2.30 4NZF[A,B,C,D,E,F,G,H] β-L-Arap 2.19 4NX0[D] 2.28
BH1870		Halalkalibacterium halodurans C-125	BAB05589.1 NP_242736.1		3CC1[A,B] 2.00
β-L-arabinopyranosidase (AgaA2;SaArap27A;SAV_2186) (Arap27A)	3.2.1.88	Streptomyces avermitilis MA-4680 = NBRC 14893	BAC69897.1 NP_823362.1	Q82L26	3A21[A,B] 1.51 3A22[A,B] α-L-Arap 1.90 3A23[A,B] β-D-Galp 1.90
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
α-N-acetylgalactosaminidase (α-NAGAL)	3.2.1.49	Gallus gallus	AAA16614.1 AAE69554.1	Q90744	1KTB[A] 1.90 1KTC[A] α-D-GalpNAc 2.40
α-galactosidase A (Gla;α-GAL)	3.2.1.22	Homo sapiens	AAH02689.1 AAA51676.1 AAA52514.1 AAA52559.1 AAB64203.1 AAE66433.1 AAE69556.1 AAN92813.1 AAP35510.1 AAQ31818.1 AAQ68716.1 AAQ81221.1 AAY03282.1 AAY71885.1 AAY71886.1 AAY71887.1 AAY71888.1 AAY71889.1 AAY71890.1 AAY71891.1 AAY71892.1 AAY71893.1 AAY71894.1 AAY72531.1 AAY72532.1 AAY72533.1 AAY72534.1 AAY72535.1 AAY72536.1 AAY72537.1 AAY72538.1 AAY72539.1 AAY72540.1 ABE23230.1 ABL16360.1 ABL21591.1 ACH14962.1 ACK10792.1 BAA34059.1 BAD96347.1 BAF83784.1 BAG59647.1 BAT62474.1 CAA29232.1 CAA32617.1 CAB55878.1 CAC09605.1 CAE85447.1 CAV33302.1 NP_000160.1	A0A0S3Q2A7 B4DLT5 P06280 Q53HF3 Q53Y83	1R46[A,B] 3.25 1R47[A,B] α-D-Galp 3.45 3GXN[A,B] 3.01 3GXP[A,B] D-1-deoxy-GlcpNAc D-1-deoxy-Manp α-D-Galp 2.20 3GXT[A,B] D-1-deoxy-GlcpNAc β-D-Manp-(1-3)-D-1-deoxy-Manp <carb_like_NOJ> 2.70 3HG2[A,B] β-D-Galp 2.30 3HG3[A,B] α-D-Galp-(1-6)-α-D-Glcp 1.90 3HG4[A,B] β-D-Glcp β-D-Talp2F-(1-4)-ASP 2.30 3HG5[A,B] α-D-Galp β-D-Galp 2.30 3LX9[A,B] α-D-GalpNAc 2.04 3LXA[A,B] α-D-Galp 3.04 3LXB[A,B] 2.85 3LXC[A,B] 2.35 3S5Y[A,B] <carb_like_DGJ> 2.10 3S5Z[A,B] α-D-Galp β-D-Galp 2.00 3TV8[A,B] <carb_like_DGJ> 2.64 4NXS[A,B] <carb_like_2OZ> 2.55 6IBK[A,B] 1.99 6IBM[A,B] 2.07 6IBR[A,B] 2.02 6IBT[A,B] 2.04
α-N-acetylgalactosaminidase / α-galactosidase B (bK250D10.5;NagA;α-NAGAL)	3.2.1.22 3.2.1.49	Homo sapiens	AAA36351.1 AAA51677.1 AAA59902.1 AAB06718.1 AAE69555.1 AAH00095.1 ACM80412.1 ACM85401.1 ASU96594.1 CAB41237.1 CAG30413.1 CAX14869.1 CAX14870.1 NP_000253.1	A0A024R1Q5 A0A248RGB1 P17050	3H53[A,B] 2.01 3H54[A,B] α-D-GalpNAc 2.20 3H55[A,B] α-D-Galp 1.91 3IGU[A,B] β-D-Talp2F-(1-4)-ASP 2.15 4DO4[A,B] <carb_like_DJN> 1.40 4DO5[A,B] <carb_like_DGJ> 1.51 4DO6[A,B] α-D-Glcp β-D-Glcp 1.60
α-galactosidase	3.2.1.22	Nicotiana benthamiana		A0A384E148	6F4C[B] 2.80
α-galactosidase I (Os10g0493600)	3.2.1.22	Oryza sativa Japonica Group	BAB12570.1 AAM92832.1 AAO85428.1 AAP54412.1 ABB47819.1 BAF26853.1 BAT11449.1 BAT11450.1 BAT11451.1 BAT11452.1 NP_922125.1	A0A0N7KS02 A0A0P0XVT5 A0A0P0XW07 F4MGX5 Q9FXT4 Q9FXT4	1UAS[A] α-D-Galp 1.50
α-galactosidase 1 (Mel1;ScMel1)	3.2.1.22	Saccharomyces cerevisiae	CAA26888.1 AAE69557.1 CBY84983.1 CBY84984.1 CUW00903.1 CUW00905.1 CUW00906.1	F4N9N8 P04824	3LRK[A] 1.95 3LRL[A] α-D-Galp-(1-6)-β-D-Glcp 2.40 3LRM[A,B,C,D] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf 2.70
α-galactosidase (Agl1)	3.2.1.22	Trichoderma reesei		Q92456	1T0O[A] β-D-Galp 1.96 1SZN[A] 1.54
α-galactosidase I (GalI;UvGalI)	3.2.1.22	Umbelopsis vinacea			3A5V[A] 2.00
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