Glycoside Hydrolase Family 27

Activities in Familyα-galactosidase (EC 3.2.1.22); α-N-acetylgalactosaminidase (EC 3.2.1.49); isomalto-dextranase (EC 3.2.1.94); β-L-arabinopyranosidase (EC 3.2.1.88); galactan:galactan galactosyltransferase (EC 2.4.1.-)
Mechanism Retaining
ClanGH-D
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorAsp
NoteMechanistic commonality with family GH36 demonstrated by Comfort et al. (2007) Biochemistry 46:3319-3330 (PMID: 17323919).
External resourcesCAZypedia; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (2108); Uniprot accession (327); PDB accession (51); 3D entries (14); cryst (0)
All (1852) Archaea (1) Bacteria (1429) Eukaryota (417) Viruses (1) unclassified (4) Structure (14) Characterized (70)
| 1 | 2 | 3 | 4 | 5 |
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3D
 α-N-acetylgalactosaminidase (NagaB) (fragment)   Salmo salar ACI33826.1 B5X3E5  
 AgaL   Salmo salar ACN10914.1 C0HA45  
 NagaB   Salmo salar ACN10976.1 C0HAA7  
 BT072612 (fragment)   Salmo salar ACN60308.1 C0PUN5  
 ORF   Salvia miltiorrhiza AAU86897.1 Q5XTZ3  
 α-N-acetylgalactosaminidase (NagA)   Schistosoma japonicum CAX70042.1
AAW26732.1
CAX70041.1
CAX70043.1
Q5DBS4  
 unknown (fragment)   Schistosoma japonicum AAX25755.1
AAX25755.2
Q5C3M0  
 SJCHGC03011 (fragment)   Schistosoma japonicum AAX25683.2 Q5C3U2  
 Smp_089290   Schistosoma mansoni Puerto Rico CAY17477.1
CCD80298.1
C1M0A5  
 Smp_170830 (fragment)   Schistosoma mansoni Puerto Rico CAY17473.1
CCD80294.1
C1M097  
 Smp_089270 (fragment)   Schistosoma mansoni Puerto Rico CAY17474.1
CCD80295.1
C1M098  
 Smp_170840 (fragment)   Schistosoma mansoni Puerto Rico CAY17475.1
CCD80296.1
C1M099  
 Smp_179260 (partial)   Schistosoma mansoni Puerto Rico CAZ37305.1
CCD80291.1
C4QPB8  
 Smp_179260 (partial)   Schistosoma mansoni Puerto Rico CAZ37305.1
CCD80291.1
C4QPB8  
 Smp_179250   Schistosoma mansoni Puerto Rico CAZ37304.1
CCD80292.1
C4QPB7  
 Smp_186710 (fragment)   Schistosoma mansoni Puerto Rico CAZ38188.1
CCD80293.1
C4QRV1  
 α-galactosidase (Mel1;SPAC869.07c) 3.2.1.22 Schizosaccharomyces pombe 972h- CAB60017.1
NP_595012.1
Q9URZ0  
 sscle_03g025620   Sclerotinia sclerotiorum 1980 UF-70 APA07792.1    
 sscle_02g015110   Sclerotinia sclerotiorum 1980 UF-70 APA06741.1    
 sscle_01g005950   Sclerotinia sclerotiorum 1980 UF-70 APA05825.1    
 sscle_07g056960   Sclerotinia sclerotiorum 1980 UF-70 APA10926.1    
 SMAX5B_009290   Scophthalmus maximus AWP11824.1    
 SMAX5B_008254   Scophthalmus maximus AWP19888.1    
 ORF (fragment)   Secale cereale ACJ06604.1 B6VCK1  
 unnamed protein product   Senna occidentalis CAA03733.1    
 PIIN_05502   Serendipita indica DSM 11827 CCA71565.1    
 PIIN_08399   Serendipita indica DSM 11827 CCA74446.1    
 PIIN_08905   Serendipita indica DSM 11827 CCA74935.1    
 α-galactosidase (LeaGal) 3.2.1.22 Solanum lycopersicum AAF04591.1
AAG16693.1
Q9FUR8
Q9SP05
 
 SRS1_15386   Sporisorium reilianum f. sp. reilianum SRS1_H2-8 SJX64569.1    
 SRS1_15067   Sporisorium reilianum f. sp. reilianum SRS1_H2-8 SJX64426.1    
 sr15067   Sporisorium reilianum SRZ2 CBQ68784.1    
 sr15386   Sporisorium reilianum SRZ2 CBQ68925.1    
 SPSC_05590   Sporisorium scitamineum SscI8 CDR88758.1    
 SPSC_04760   Sporisorium scitamineum SscI8 CDU24927.1    
 SRAE_1000106900   Strongyloides ratti ED321 Heterogonic CEF62800.1    
 α-galactosidase A   Sus scrofa ACD67895.1
ACD67896.1
D5FGE4  
 α-N-acetylgalactosaminidase (NagA)   Sus scrofa ACA43013.1 B1PK16  
 Agal   Takifugu rubripes CAC44626.1 Q90WL7  
 α-galactosidase (Aga27A) 3.2.1.22 Talaromyces leycettanus JCM12802 AJF11712.1    
 EYB26_006888   Talaromyces marneffei QGA19200.1    
 EYB26_004163   Talaromyces marneffei QGA16496.1    
 α-galactosidase (aGal) 3.2.1.22 Talaromyces purpureogenus 618 BAA22992.1 O13295  
 α-galactopyranosidase (Gla1;GLA1)   Talaromyces purpureogenus ATCC MYA-38 AKH40275.1    
 α-N-acetylgalactosaminidase   Tetranychus truncatus AYV89050.1    
 ORF   Tetranychus truncatus AYV89021.1    
 α-galactosidase (TcGal27A) (Gal27A) 3.2.1.22 Thermoascus crustaceus JCM12803 ATO91445.1    
 MYCTH_2119446 (Aga1)   Thermothelomyces thermophilus ATCC 42464 AEO59170.1    
 MYCTH_2313137 (Aga2)   Thermothelomyces thermophilus ATCC 42464 AEO62124.1    
 α-galactosidase (Aga2)   Thermothelomyces thermophilus C1 AFJ59927.1    
 α-galactosidase (Aga1;aGal1) 3.2.1.22 Thermothelomyces thermophilus C1 AFJ59926.1    
 THITE_2124844   Thermothielavioides terrestris NRRL 8126 AEO71949.1    
 α-galactosidase   Thielavia terrestris CAU709 ARI48112.1    
 α-galactosidase (MELt) 3.2.1.22 Torulaspora delbrueckii BAA86883.1 Q9UVD6  
 TDEL_0E00170 (Tdel0e00170) (fragment)   Torulaspora delbrueckii type strain:CBS 1146 CCE92260.1    
 α-galactosidase (Agl1) 3.2.1.22 Trichoderma reesei   Q92456 1T0O[A]
1SZN[A]
 α-galactosidase 1 (Agl1;AGLI) 3.2.1.22 Trichoderma reesei RUTC-30 CAA93244.1 Q92456  
 α-galactosidase 3 (Agl3;AGLIII) 3.2.1.22 Trichoderma reesei RUTC-30 CAA93246.1 Q92451  
 CAMPLR22A2D_LOCUS929   Triticum aestivum SPT16333.1    
 Sequence 105792 from patent US 7214786   Triticum aestivum ABT18322.1    
 Sequence 107403 from patent US 7214786   Triticum aestivum ABT19933.1    
 ORF (fragment)   Triticum monococcum ACJ06602.1 B6VCJ9  
 ORF (fragment)   Triticum urartu ACJ06601.1 B6VCJ8  
 α-galactosidase I (GalI;UvGalI) 3.2.1.22 Umbelopsis vinacea     3A5V[A]
 α-galactosidase II 3.2.1.22 Umbelopsis vinacea BAA33931.1 O93816  
 α-galactosidase 3.2.1.22 Umbelopsis vinacea AAB35252.2 Q02402  
 UBRO_06272   Ustilago bromivora UB2112 SAM83953.1    
 FKW77_004773   Venturia effusa albino QDS73740.1    
 FKW77_004936   Venturia effusa albino QDS72189.1    
 FKW77_002637   Venturia effusa albino QDS77633.1    
 FKW77_008431   Venturia effusa albino QDS73423.1    
 FKW77_000775   Venturia effusa albino QDS74720.1    
 α-galactosidase   Vernicia fordii ARV78455.1    
 VIGAN_09002800 (Vigan.09g002800) (fragment)   Vigna angularis var. angularis BAT96745.1    
 VIGAN_11209000 (Vigan.11g209000)   Vigna angularis var. angularis BAU02541.1    
 VIGAN_01092100 (Vigan.01g092100)   Vigna angularis var. angularis BAT73439.1    
 VIGAN_04422100 (Vigan.04g422100)   Vigna angularis var. angularis BAT86554.1    
 VIGAN_02181800 (Vigan.02g181800)   Vigna angularis var. angularis BAT79025.1    
 VIGAN_04113100 (Vigan.04g113100) (fragment)   Vigna angularis var. angularis BAT83891.1    
 VIGAN_04114600 (Vigan.04g114600)   Vigna angularis var. angularis BAT83904.1    
 DEO72_LG2g4260 (fragment)   Vigna unguiculata QCD83911.1    
 DEO72_LG2g5009   Vigna unguiculata QCD84654.1    
 DEO72_LG11g2359   Vigna unguiculata QCE15349.1    
 DEO72_LG7g2734   Vigna unguiculata QCE01437.1    
 DEO72_LG10g3364   Vigna unguiculata QCE12123.1    
 DEO72_LG6g872 (fragment)   Vigna unguiculata QCD96170.1    
 DEO72_LG5g3100   Vigna unguiculata QCD95010.1    
 DEO72_LG5g2393 (fragment)   Vigna unguiculata QCD94310.1    
 DEO72_LG8g2712   Vigna unguiculata QCE04674.1    
 DEO72_LG11g139   Vigna unguiculata QCE13146.1    
 VITISV_043596   Vitis vinifera CAN75523.1 A5ACL5  
 VITISV_001661 (fragment)   Vitis vinifera CAN75822.1 A5AM08  
 VITISV_000962   Vitis vinifera CAN66885.1 A5ALZ9  
 VITISV_041251   Vitis vinifera CAN81856.1 A5C5Q1  
 α-galactosidase   Xanthophyllomyces dendrorhous CED85437.1    
 Unknown (protein for IMAGE:5156001)   Xenopus laevis AAH72931.1 Q6GQ17  
 Unknown (protein for MGC:81044)   Xenopus laevis AAH71089.1 Q6GR44  
 Unknown (protein for MGC:130872)   Xenopus laevis AAI06338.1 Q3KQ87  
 LOC100145767 protein   Xenopus tropicalis AAI61735.1 B1WBF4  
 Unknown (protein for IMAGE:7874727)   Xenopus tropicalis AAI54864.1
AAI22060.1
A8WGV2  
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