Glycoside Hydrolase Family 27

Known Activities	α-galactosidase (EC 3.2.1.22); α-N-acetylgalactosaminidase (EC 3.2.1.49); isomalto-dextranase (EC 3.2.1.94); β-L-arabinopyranosidase (EC 3.2.1.88)
Mechanism	Retaining
Clan	GH-D
3D Structure Status	( β / α ) ₈
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Asp
Note	Mechanistic commonality with family GH36 demonstrated by Comfort et al. (2007) Biochemistry 46:3319-3330 (PMID: 17323919).
External resources	CAZypedia; InterPro; PFAM; PRINTS; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (634); Uniprot accession (304); PDB accession (33); 3D entries (10); cryst (1)

Summary

All (459) Archaea (1) Bacteria (229) Eukaryota (228) unclassified (1) Structure (10 - 1 cryst) Characterized (66)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
isomaltodextranase (ImdA)	3.2.1.94	Arthrobacter dextranolyticus T6	BAD34648.1	Q6BE01
isomaltodextranase (G2d;Imd)	3.2.1.94	Arthrobacter globiformis T6	BAA06424.1 BAC76886.2	Q44052 Q7WSN5	cryst
α-galactosidase 27A (Aga27A)	3.2.1.22	Cellvibrio mixtus ATCC 12120	AAS19696.1	Q6QT41
galactosidase (Ccel_1237) (Gal27A)	3.2.1.22	Clostridium cellulolyticum H10 ATCC 35319	ABG76969.1 ACL75593.1	B8I0L8
α-galactosidase (AgaA) (Aga27A)	3.2.1.22	Clostridium josui FERM P-9684	BAB83765.1	Q8VV86
β-L-arabinopyranosidase (Abp)	3.2.1.88	Geobacillus stearothermophilus T-6 NCIMB 40222
α-galactosidase (MelA;AgaB2;SACE_3139)	3.2.1.22	Saccharopolyspora erythraea NRRL 2338	AAC99325.1 CAM02417.1	Q9Z4N5
β-L-arabinopyranosidase (AgaA2;SaArap27A;SAV_2186) (Arap27A)	3.2.1.88	Streptomyces avermitilis MA-4680	BAC69897.1 NP_823362.1		3A21[A,B] 3A22[A,B] 3A23[A,B]
α-galactosidase I (α-GalI) (peptide fragment)	3.2.1.22	Streptomyces griseoloalbus
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
[Blood group A] α-N-acetylgalactosaminidase (NagA)	3.2.1.49	Acremonium sp. No.413	BAB08149.1	Q9HFB1
galactan:galactan galactosyltransferase 1 (GGT-1)	2.4.1.-	Ajuga reptans	AAR02007.1	Q6TW99
α-galactosidase B (AglB)	3.2.1.22	Aspergillus fumigatus IMI 385708	ACO72591.1	C3V6M2 Q4WE86
α-galactosidase (AN7152.2)	3.2.1.22	Aspergillus nidulans FGSC A4	ABF50881.1 EAA61404.1
α-galactosidase B (AglB)	3.2.1.22	Aspergillus niger CBS 120.49 / N400	CAB46229.1	Q9Y865
α-N-acetylgalactosaminidase / α-galactosidase (AglA;An06g00170)	3.2.1.22 3.2.1.49	Aspergillus niger CBS 513.88	CAK44933.1	A2QL72
α-galactosidase II (AglB;An02g11150) (Gal27B)	3.2.1.22	Aspergillus niger CBS 513.88	CAK44445.1	A2QEJ9
α-N-acetylgalactosaminidase (fragment)	3.2.1.49	Aspergillus niger CCIM K2	AEQ39734.1
α-galactosidase A (Ag1A;AglA)	3.2.1.22	Aspergillus niger N402	AAE69559.1 CAA44950.1	P28351.1
α-galactosidase (AgalB)	3.2.1.22	Bispora sp. MEY-1	ADN30030.1	E2E1R9
α-galactosidase	3.2.1.22	Coffea arabica	AAA33022.1 AAQ77888.1	Q42656
α-galactosidase (Gal1)	3.2.1.22	Coffea arabica CATURRA ROJO T2308	CAI47559.1	Q5DUH8
α-galactosidase (Gal1)	3.2.1.22	Coffea canephora 97	CAI47560.1	Q5DUH7
acid α-galactosidase 2	3.2.1.22	Cucumis sativus	ABC88435.1	Q2HYY3
α-galactosidase	3.2.1.22	Cyamopsis tetragonoloba	AAA72859.1 AAE69558.1 CAA32772.1	P14749.1
α-galactosidase (DEHA0F28490g)	3.2.1.22	Debaryomyces hansenii CBS767	CAG89932.1
α-galactosidase / β-L-arabinopyranosidase (FoAp2)	3.2.1.22 3.2.1.88	Fusarium oxysporum 12S	BAH10648.1	B7XH21
α-galactosidase / β-L-arabinopyranosidase (FoAp1)	3.2.1.22 3.2.1.88	Fusarium oxysporum 12S	BAH10649.1	B7XH22
α-N-acetylgalactosaminidase	3.2.1.49	Gallus gallus	AAA16614.1 AAE69554.1	Q90744	1KTB[A] 1KTC[A]
α-galactosidase (fragment)	3.2.1.22	Ganoderma lucidum
α-galactosidase	3.2.1.22	Glycine max	AAA73963.1 AAR65332.1 ACU23822.1	Q39811
α-galactosidase (Gla)	3.2.1.22	Helianthus annuus	BAC66445.1	Q84VQ7
α-galactosidase A (Gla;α-GAL)	3.2.1.22	Homo sapiens	AAA51676.1 AAA52514.1 AAA52559.1 AAB64203.1 AAE66433.1 AAE69556.1 AAH02689.1 AAN92813.1 AAP35510.1 AAQ31818.1 AAQ68716.1 AAQ81221.1 AAY03282.1 AAY71885.1 AAY71886.1 AAY71887.1 AAY71888.1 AAY71889.1 AAY71890.1 AAY71891.1 AAY71892.1 AAY71893.1 AAY71894.1 AAY72531.1 AAY72532.1 AAY72533.1 AAY72534.1 AAY72535.1 AAY72536.1 AAY72537.1 AAY72538.1 AAY72539.1 AAY72540.1 ABE23230.1 ABL16360.1 ABL21591.1 ACH14962.1 ACH14962.1 ACK10792.1 BAA34059.1 BAD96347.1 BAF83784.1 BAG59647.1 CAA29232.1 CAA32617.1 CAB55878.1 CAC09605.1 CAE85447.1 CAV33302.1 NP_000160.1	P06280.1 Q53Y83	1R46[A,B] 1R47[A,B] 3GXN[A,B] 3GXP[A,B] 3GXT[A,B] 3HG2[A,B] 3HG3[A,B] 3HG4[A,B] 3HG5[A,B] 3LX9[A,B] 3LXA[A,B] 3LXB[A,B] 3LXC[A,B] 3S5Y[A,B] 3S5Z[A,B] 3TV8[A,B]
α-N-acetylgalactosaminidase / α-galactosidase B (bK250D10.5;NagA;α-NAGAL)	3.2.1.22 3.2.1.49	Homo sapiens	AAA36351.1 AAA51677.1 AAA59902.1 AAB06718.1 AAE69555.1 AAH00095.1 ACM80412.1 ACM85401.1 ACM85401.1 CAB41237.1 CAG30413.1 CAX14869.1 CAX14870.1 NP_000253.1	P17050.2	3H53[A,B] 3H54[A,B] 3H55[A,B] 3IGU[A,B]
α-galactosidase (Agl1)	3.2.1.22	Hypocrea jecorina		Q92456	1SZN[A] 1T0O[A]
α-galactosidase 1 (Agl1)	3.2.1.22	Hypocrea jecorina RUTC-30	CAA93244.1	Q92456
α-galactosidase 3 (Agl3)	3.2.1.22	Hypocrea jecorina RUTC-30	CAA93246.1	Q92451
α-N-acetylgalactosaminidase (NagA)	3.2.1.49	Mus musculus	AAC28851.1 AAH21631.1 AAL87527.1 AAL87528.1 BAC36620.1 BAE21730.1 BAE31642.1 CAA11703.1 NP_032695.2	Q9QWR8 Q9QWR8.2
α-galactosidase A (Gls;Agl) (lysosomal)	3.2.1.22	Mus musculus	AAA74453.1 AAA96749.1 AAB47244.1 AAC52583.1 AAC52584.1 AAH09021.1 BAC30508.1 BAC35819.1 BAE26260.1 BAE37692.1 BAE38627.1 BAE38657.1 CAM19136.1 CAM19137.1 NP_038491.1	P51569 Q8BGZ6
α-galactosidase II (Os07g0452100)	3.2.1.22	Oryza sativa Japonica Group	BAC55816.1 BAC84411.1 BAF21463.1	Q0D6R0
α-galactosidase III (Os07g0679300)	3.2.1.22	Oryza sativa Japonica Group	BAC79549.1 BAD31216.1 BAF22553.1 BAG94006.1	Q7XIV4
α-galactosidase I (Os10g0493600)	3.2.1.22	Oryza sativa Japonica Group	AAM92832.1 AAO85428.1 AAP54412.1 ABB47819.1 BAB12570.1 BAF26853.1 NP_922125.1	Q9FXT4 Q9FXT4.1	1UAS[A]
α-galactosidase (aGal)	3.2.1.22	Penicillium purpurogenum 618	BAA22992.1	O13295
α-galactosidase I (Agl1;AglI)	3.2.1.22	Penicillium simplicissimum VTT-D-78090	CAA08915.1	O94221
α-galactosidase III (AgalIII) (peptide fragment)	3.2.1.22	Penicillium simplicissimum VTT-D-78090
α-galactosidase (AgalB)	3.2.1.22	Phanerochaete chrysosporium ME446	AAG24511.1	Q9HFZ9
α-galactosidase (AgalA)	3.2.1.22	Phanerochaete chrysosporium ME446	AAG24510.1	Q9HG00
α-galactosidase	3.2.1.22	Phaseolus vulgaris	AAA73964.1 AAR65334.1	Q41100
α-galactosidase 1 (Gal1)	3.2.1.22	Pisum sativum	CAF34023.1	Q5ZP79
α-galactosidase (MEL)	3.2.1.22	Saccharomyces carlsbergensis NCYC396	AAA34769.1	Q03647
α-galactosidase 1 (Mel1)	3.2.1.22	Saccharomyces cerevisiae	AAE69557.1 CAA26888.1 CBY84983.1 CBY84984.1	P04824.1	3LRK[A] 3LRL[A] 3LRM[A,B,C,D]
α-galactosidase 2 (Mel2)	3.2.1.22	Saccharomyces cerevisiae	CAA85737.1	P41945
α-galactosidase 5 (Mel5)	3.2.1.22	Saccharomyces cerevisiae	CAA85740.1	P41946
α-galactosidase 6 (Mel6)	3.2.1.22	Saccharomyces cerevisiae	CAA85739.1	P41947
α-galactosidase (MELj)	3.2.1.22	Saccharomyces mikatae IFO 1816	CAA64760.1	Q11129
α-galactosidase (MELp)	3.2.1.22	Saccharomyces paradoxus 61-248	CAA64759.1	Q09187
α-galactosidase (Mel1;SPAC869.07c)	3.2.1.22	Schizosaccharomyces pombe 972h-	CAB60017.1 NP_595012.1	Q9URZ0
α-galactosidase (LeaGal)	3.2.1.22	Solanum lycopersicum	AAF04591.1 AAG16693.1	Q9FUR8 Q9SP05
α-galactosidase (Gal1)	3.2.1.22	Talaromyces emersonii	ABU94728.1	A7XZT2
α-galactosidase (peptide fragments)	3.2.1.22	Thermomyces lanuginosus IMI 158749
α-galactosidase (MELt)	3.2.1.22	Torulaspora delbrueckii	BAA86883.1	Q9UVD6
α-galactosidase I (GalI;UvGalI)	3.2.1.22	Umbelopsis vinacea			3A5V[A]
α-galactosidase II	3.2.1.22	Umbelopsis vinacea	BAA33931.1	O93816
α-galactosidase	3.2.1.22	Umbelopsis vinacea	AAB35252.2	Q02402
α-N-acetylgalactosaminidase I (peptide fragment)	3.2.1.49	uncultured eukaryote (squid)
α-N-acetylgalactosaminidase II (peptide fragment)	3.2.1.49	uncultured eukaryote (squid)
α-galactosidase (MELr)	3.2.1.22	Zygosaccharomyces mrakii IFO 1835	BAA99555.1	Q9P4V4

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.