Glycoside Hydrolase Family 27

Activities in Familyα-galactosidase (EC 3.2.1.22); α-N-acetylgalactosaminidase (EC 3.2.1.49); isomalto-dextranase (EC 3.2.1.94); β-L-arabinopyranosidase (EC 3.2.1.88); galactan:galactan galactosyltransferase (EC 2.4.1.-)
Mechanism Retaining
ClanGH-D
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorAsp
NoteMechanistic commonality with family GH36 demonstrated by Comfort et al. (2007) Biochemistry 46:3319-3330 (PMID: 17323919).
External resourcesCAZypedia; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (3309); Uniprot accession (93); PDB accession (51); 3D entries (14); cryst (0)
Bacteria
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 isomaltodextranase (ImdA) 3.2.1.94 pubmed
Arthrobacter dextranolyticus T6 BAD34648.1 Q6BE01  
 isomalto-dextranase (G2d;Imd) 3.2.1.94 pubmed
Arthrobacter globiformis T6 BAC76886.2
BAA06424.1
Q44052
Q7WSN5
5AWO[A]
5AWP[A]
5AWQ[A]
 α-galactosidase 3.2.1.22 pubmed
Bacillus sp. N16-5 AML27059.1 A0A140EH88  
 α-galactosidase (BT4157;BT_4157) 3.2.1.22 pubmed
Bacteroides thetaiotaomicron VPI-5482 AAO79262.1
NP_813068.1
Q8A064  
 α-galactosidase 27A (Aga27A) 3.2.1.22   Cellvibrio mixtus ATCC 12120 AAS19696.1 Q6QT41  
 β-L-arabinopyranosidase (CpArap27;Cpin_5740) 3.2.1.88 pubmed
Chitinophaga pinensis DSM 2588 ACU63161.1 C7PCU7  
 β-L-arabinopyranosidase (Abp;HUS_CDS23) 3.2.1.88 pubmed
Geobacillus stearothermophilus T-6 NCIMB 40222     4NX0[A,B,C,E,F,G,H]
4NXK[A,B,C,D,E,F,G,H]
4NZF[A,B,C,D,E,F,G,H]
4NX0[D]
 α-galactosidase (Ccel_1237) (Gal27A) 3.2.1.22 pubmed
Ruminiclostridium cellulolyticum H10 ACL75593.1
ABG76969.1
B8I0L8
Q0PRN2
 
 α-galactosidase (AgaA) (Aga27A) 3.2.1.22 pubmed
Ruminiclostridium josui FERM P-9684 BAB83765.1 Q8VV86  
 α-galactosidase (MelA;AgaB2;SACE_3139) 3.2.1.22 pubmed
Saccharopolyspora erythraea NRRL 2338 AAC99325.1
CAM02417.1
A4FEE2
Q9Z4N5
 
 β-L-arabinopyranosidase (AgaA2;SaArap27A;SAV_2186) (Arap27A) 3.2.1.88 pubmed
Streptomyces avermitilis MA-4680 = NBRC 14893 BAC69897.1
NP_823362.1
Q82L26 3A21[A,B]
3A22[A,B]
3A23[A,B]
Eukaryota
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 [Blood group A] α-N-acetylgalactosaminidase (NagA) 3.2.1.49 doi
Acremonium sp. No.413 BAB08149.1 Q9HFB1  
 galactan:galactan galactosyltransferase 1 (GGT-1) 2.4.1.- pubmed
Ajuga reptans AAR02007.1 Q6TW99  
 α-galactosidase 27A (Gal27A) 3.2.1.22 pubmed
Aspergillus fischeri P1 / CGMCC 3.15369 AGV79321.1
AGV79322.1
A0A023IMK0
A0A088BDE2
 
 α-galactosidase 27B (Gal27B) 3.2.1.22 pubmed
Aspergillus fischeri P1 / CGMCC 3.15369 AJA29661.1 A0A0A7R517  
 α-galactosidase B (AglB) 3.2.1.22 doi
Aspergillus fumigatus IMI 385708 ACO72591.1 C3V6M2
Q4WE86
 
 α-galactosidase (AN7152.2) 3.2.1.22   Aspergillus nidulans FGSC A4 ABF50881.1
EAA61404.1
Q5AX28  
 α-galactosidase B (AglB) 3.2.1.22 pubmed
Aspergillus niger CBS 120.49 / N400 CAB46229.1 Q9Y865  
 α-N-acetylgalactosaminidase / α-galactosidase (AglA;An06g00170) 3.2.1.49 pubmed
Aspergillus niger CBS 513.88 CAK44933.1 A2QL72  
 α-galactosidase II (AglB;An02g11150) (Gal27B) 3.2.1.22 pubmed
Aspergillus niger CBS 513.88 CAK44445.1 A2QEJ9  
 α-N-acetylgalactosaminidase (partial) 3.2.1.49 pubmed
Aspergillus niger CCIM K2 AEQ39734.1 G5D7B5  
 α-galactosidase A (Ag1A;AglA) 3.2.1.22 pubmed
Aspergillus niger N402 CAA44950.1
AAE69559.1
P28351  
 α-galactosidase (AglB) 3.2.1.22 pubmed
Aspergillus niger No.499 BAU20370.1 A0A0U5AE32  
 α-galactosidase (AgalB) 3.2.1.22 pubmed
Bispora sp. MEY-1 ADN30030.1 E2E1R9  
 α-galactosidase 3.2.1.22 pubmed
Coffea arabica AAA33022.1
AAQ77888.1
Q42656  
 α-galactosidase (Gal1) 3.2.1.22 pubmed
Coffea arabica CATURRA ROJO T2308 CAI47559.1 Q5DUH8  
 α-galactosidase (Gal1) 3.2.1.22 pubmed
Coffea canephora 97 CAI47560.1 Q5DUH7  
 α-galactosidase (fragment) 3.2.1.22   Colocasia esculenta MQL72187.1    
 acid α-galactosidase 2 3.2.1.22 doi
Cucumis sativus ABC88435.1 Q2HYY3  
 α-galactosidase (Aga27A) 3.2.1.22 pubmed
Cyamopsis tetragonoloba CAA32772.1
AAA72859.1
AAE69558.1
P14749  
 α-galactosidase (DEHA0F28490g) 3.2.1.22 pubmed
Debaryomyces hansenii CBS767 CAG89932.1 Q6BJW5  
 α-galactosidase (Aga27A) 3.2.1.22 pubmed
Evansstolkia leycettana JCM12802 AJF11712.1 A0A0H3V1A2  
 α-galactosidase / β-L-arabinopyranosidase (FoAp2) 3.2.1.22
3.2.1.88
pubmed
pubmed
Fusarium oxysporum 12S BAH10648.1 B7XH21  
 α-galactosidase / β-L-arabinopyranosidase (FoAp1) 3.2.1.22
3.2.1.88
pubmed
pubmed
Fusarium oxysporum 12S BAH10649.1 B7XH22  
 α-N-acetylgalactosaminidase (α-NAGAL) 3.2.1.49 pubmed
Gallus gallus AAA16614.1
AAE69554.1
Q90744 1KTB[A]
1KTC[A]
 α-galactosidase (Gla) 3.2.1.22 pubmed
Helianthus annuus BAC66445.1 Q84VQ7  
 α-galactosidase A (Gla;α-GAL) 3.2.1.22 pubmed
Homo sapiens AAH02689.1
AAA51676.1
AAA52514.1
AAA52559.1
AAB64203.1
AAE66433.1
AAE69556.1
AAN92813.1
AAP35510.1
AAQ31818.1
AAQ68716.1
AAQ81221.1
AAY03282.1
AAY71885.1
AAY71886.1
AAY71887.1
AAY71888.1
AAY71889.1
AAY71890.1
AAY71891.1
AAY71892.1
AAY71893.1
AAY71894.1
AAY72531.1
AAY72532.1
AAY72533.1
AAY72534.1
AAY72535.1
AAY72536.1
AAY72537.1
AAY72538.1
AAY72539.1
AAY72540.1
ABE23230.1
ABL16360.1
ABL21591.1
ACH14962.1
ACK10792.1
BAA34059.1
BAD96347.1
BAF83784.1
BAG59647.1
BAT62474.1
CAA29232.1
CAA32617.1
CAB55878.1
CAC09605.1
CAE85447.1
CAV33302.1
NP_000160.1
A0A0S3Q2A7
B4DLT5
P06280
Q53HF3
Q53Y83
1R46[A,B]
1R47[A,B]
3GXN[A,B]
3GXP[A,B]
3GXT[A,B]
3HG2[A,B]
3HG3[A,B]
3HG4[A,B]
3HG5[A,B]
3LX9[A,B]
3LXA[A,B]
3LXB[A,B]
3LXC[A,B]
3S5Y[A,B]
3S5Z[A,B]
3TV8[A,B]
4NXS[A,B]
6IBK[A,B]
6IBM[A,B]
6IBR[A,B]
6IBT[A,B]
 α-N-acetylgalactosaminidase / α-galactosidase B (bK250D10.5;NagA;α-NAGAL) 3.2.1.22
3.2.1.49
pubmed
Homo sapiens AAA36351.1
AAA51677.1
AAA59902.1
AAB06718.1
AAE69555.1
AAH00095.1
ACM80412.1
ACM85401.1
ASU96594.1
CAB41237.1
CAG30413.1
CAX14869.1
CAX14870.1
NP_000253.1
A0A024R1Q5
A0A248RGB1
P17050
3H53[A,B]
3H54[A,B]
3H55[A,B]
3IGU[A,B]
4DO4[A,B]
4DO5[A,B]
4DO6[A,B]
 α-N-acetylgalactosaminidase (NagA) 3.2.1.49   Mus musculus AAC28851.1
AAH21631.1
AAL87527.1
AAL87528.1
BAC36620.1
BAE21730.1
BAE31642.1
CAA11703.1
NP_032695.2
Q3U6T2
Q3UZX5
Q9QWR8
Q9QWR8
 
 α-galactosidase A (Gls;Agl) (lysosomal) 3.2.1.22 pubmed
Mus musculus AAA74453.1
AAA96749.1
AAB47244.1
AAC52583.1
AAC52584.1
AAH09021.1
BAC30508.1
BAC35819.1
BAE26260.1
BAE37692.1
BAE38627.1
BAE38657.1
CAM19136.1
CAM19137.1
NP_038491.1
P51569
Q3TLY5
Q3UM38
Q8BGZ6
 
 α-galactosidase 3.2.1.22 pubmed
Nicotiana benthamiana   A0A384E148 6F4C[B]
 α-galactosidase II (Os07g0452100) 3.2.1.22 pubmed
Oryza sativa Japonica Group BAF21463.1
BAC55816.1
BAC84411.1
BAT01318.1
A0A0P0X580
Q0D6R0
Q84Q36
 
 α-galactosidase III (Os07g0679300) 3.2.1.22 pubmed
Oryza sativa Japonica Group BAC79549.1
BAD31216.1
BAF22553.1
BAG94006.1
BAT03227.1
Q69R81
Q7XIV4
 
 α-galactosidase I (Os10g0493600) 3.2.1.22 pubmed
Oryza sativa Japonica Group BAB12570.1
AAM92832.1
AAO85428.1
AAP54412.1
ABB47819.1
BAF26853.1
BAT11449.1
BAT11450.1
BAT11451.1
BAT11452.1
NP_922125.1
A0A0N7KS02
A0A0P0XVT5
A0A0P0XW07
F4MGX5
Q9FXT4
Q9FXT4
1UAS[A]
 α-galactosidase I (Agl1;AglI) 3.2.1.22 pubmed
Penicillium simplicissimum VTT-D-78090 CAA08915.1 O94221  
 α-galactosidase (AgalB) 3.2.1.22 pubmed
Phanerochaete chrysosporium ME446 AAG24510.1
AAG24511.1
Q9HFZ9
Q9HG00
 
 α-galactosidase 3.2.1.22 doi
Phaseolus vulgaris AAA73964.1
AAR65334.1
Q41100  
 α-galactosidase 1 (Gal1) 3.2.1.22 pubmed
Pisum sativum CAF34023.1 Q5ZP79  
 α-galactosidase (Gal1) 3.2.1.22 pubmed
Rasamsonia emersonii ABU94728.1 A7XZT2  
 α-galactosidase (MEL) 3.2.1.22 pubmed
Saccharomyces carlsbergensis NCYC396 AAA34769.1 Q03647  
 α-galactosidase 1 (Mel1;ScMel1) 3.2.1.22 pubmed
Saccharomyces cerevisiae CAA26888.1
AAE69557.1
CBY84983.1
CBY84984.1
CUW00903.1
CUW00905.1
CUW00906.1
F4N9N8
P04824
3LRK[A]
3LRL[A]
3LRM[A,B,C,D]
 α-galactosidase (Mel1;SPAC869.07c) 3.2.1.22 pubmed
Schizosaccharomyces pombe 972h- CAB60017.1
NP_595012.1
Q9URZ0  
 α-galactosidase (LeaGal) 3.2.1.22 pubmed
Solanum lycopersicum AAF04591.1
AAG16693.1
Q9FUR8
Q9SP05
 
 α-galactosidase (aGal) 3.2.1.22 pubmed
Talaromyces purpureogenus 618 BAA22992.1 O13295  
 α-galactosidase (TcGal27A) (Gal27A) 3.2.1.22   Thermoascus crustaceus JCM12803 ATO91445.1 A0A2D1PE30  
 α-galactosidase (Aga1;aGal1) 3.2.1.22 pubmed
Thermothelomyces thermophilus C1 AFJ59926.1 I2C093  
 α-galactosidase (Agl1) 3.2.1.22 pubmed
Trichoderma reesei   Q92456 1T0O[A]
1SZN[A]
 α-galactosidase 1 (Agl1;AGLI) 3.2.1.22 pubmed
Trichoderma reesei RUTC-30 CAA93244.1 Q92456  
 α-galactosidase 3 (Agl3;AGLIII) 3.2.1.22 pubmed
Trichoderma reesei RUTC-30 CAA93246.1 Q92451  
 α-galactosidase I (GalI;UvGalI) 3.2.1.22 doi
Umbelopsis vinacea     3A5V[A]
 α-galactosidase II 3.2.1.22 pubmed
Umbelopsis vinacea BAA33931.1 O93816  
 α-galactosidase 3.2.1.22 pubmed
Umbelopsis vinacea AAB35252.2 Q02402  
unclassified
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 α-galactosidase (GalR) 3.2.1.22 pubmed
synthetic construct QCC19990.1
QCC19991.1
   
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