Glycoside Hydrolase Family 27

Activities in Familyα-galactosidase (EC 3.2.1.22); α-N-acetylgalactosaminidase (EC 3.2.1.49); isomalto-dextranase (EC 3.2.1.94); β-L-arabinopyranosidase (EC 3.2.1.88); galactan:galactan galactosyltransferase (EC 2.4.1.-)
Mechanism Retaining
ClanGH-D
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp
Catalytic Proton DonorAsp
NoteMechanistic commonality with family GH36 demonstrated by Comfort et al. (2007) Biochemistry 46:3319-3330 (PMID: 17323919).
External resourcesCAZypedia; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (1562); Uniprot accession (327); PDB accession (47); 3D entries (14); cryst (0)
All (1305) Archaea (1) Bacteria (955) Eukaryota (347) Viruses (1) unclassified (1) Structure (14) Characterized (69)
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Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D
 P350_36305   Burkholderia cepacia JBK9 ALX17362.1    
 P350_36305   Burkholderia cepacia JBK9 ALX17362.1    
 BM43_6103   Burkholderia gladioli ATCC 10248 AJW95302.1    
 BM43_3411   Burkholderia gladioli ATCC 10248 AJW98162.1    
 bgla_2g24110   Burkholderia gladioli BSR3 AEA64841.1    
 A8H28_10015   Burkholderia gladioli pv. gladioli FDAARGOS_188 AWY51490.1    
 A8H28_30970   Burkholderia gladioli pv. gladioli FDAARGOS_188 AWY55415.1    
 CO712_03740   Burkholderia gladioli pv. gladioli FDAARGOS_389 ATF84254.1    
 CO712_26520   Burkholderia gladioli pv. gladioli FDAARGOS_389 ATF88570.1    
 CEJ98_10190   Burkholderia gladioli pv. gladioli KACC 11889 ASD79344.1    
 CEJ98_35250   Burkholderia gladioli pv. gladioli KACC 11889 ASD84067.1    
 bglu_2g20960   Burkholderia glumae BGR1 ACR32404.1 C5AKH4  
 KS03_3832   Burkholderia glumae LMG 2196 = ATCC 33617 AJY62634.1    
 BG90_652 (fragment)   Burkholderia oklahomensis C6786 AJX30412.1
AOI46807.1
AOI46807.1
   
 WG70_27430 (fragment)   Burkholderia oklahomensis EO147 AOI43237.1    
 DM82_376 (fragment)   Burkholderia oklahomensis EO147 AIO65421.1    
 WS54_28685   Burkholderia sp. NRF60-BP8 AOI80898.1    
 AXG89_34120   Burkholderia sp. PAMC 26561 AMH42854.1    
 AX768_18390   Burkholderia sp. PAMC 28687 AMM16180.1    
 BBJ41_36430   Burkholderia stabilis ATCC BAA-67 AOR73446.1    
 CIY_22650   Butyrivibrio fibrisolvens 16/4 CBK74937.1 D4IVZ4  
 bhn_I1287   Butyrivibrio hungatei MB2003 AOZ96321.1    
 bhn_I0082   Butyrivibrio hungatei MB2003 AOZ95118.1    
 bpr_I0090   Butyrivibrio proteoclasticus B316 ADL32841.1 E0RUF1  
 bpr_I0205   Butyrivibrio proteoclasticus B316 ADL32954.1 E0RW88  
 Cabys_2804   Caldithrix abyssi DSM 13497 LF13 APF19552.1    
 Acid345_3249   Candidatus Koribacter versatilis Ellin345 ABF42250.1 Q1ILK0  
 Acid345_1677   Candidatus Koribacter versatilis Ellin345 ABF40678.1 Q1IR22  
 Acid_0705   Candidatus Solibacter usitatus Ellin6076 ABJ81706.1 Q02B60  
 Acid_1472   Candidatus Solibacter usitatus Ellin6076 ABJ82464.1 Q028T8  
 Acid_1588   Candidatus Solibacter usitatus Ellin6076 ABJ82579.1 Q028H3  
 Acid_2820   Candidatus Solibacter usitatus Ellin6076 ABJ83806.1 Q023N4  
 CGC52_01050 (fragment)   Capnocytophaga sp. H2931 ATA74150.1    
 CGC49_01070 (fragment)   Capnocytophaga sp. H4358 ATA72030.1    
 C2869_05770   Catenovulum sp. CCB-QB4 AWB65978.1    
 C2869_03415   Catenovulum sp. CCB-QB4 AWB65540.1    
 C2869_07730   Catenovulum sp. CCB-QB4 AWB68961.1    
 C2869_07570   Catenovulum sp. CCB-QB4 AWB66299.1    
 C2869_00025 (fragment)   Catenovulum sp. CCB-QB4 AWB64924.1    
 Caci_2554   Catenulispora acidiphila DSM 44928 ACU71472.1 C7PXL9  
 Caci_4439   Catenulispora acidiphila DSM 44928 ACU73303.1 C7PW55  
 Caci_4641   Catenulispora acidiphila DSM 44928 ACU73502.1 C7PY42  
 Caci_4912   Catenulispora acidiphila DSM 44928 ACU73772.1 C7Q336  
 Caci_6873   Catenulispora acidiphila DSM 44928 ACU75712.1 C7Q3E7  
 Caci_7918   Catenulispora acidiphila DSM 44928 ACU76741.1 C7QFG4  
 Caci_4880   Catenulispora acidiphila DSM 44928 ACU73740.1 C7Q1L1  
 Caci_5007   Catenulispora acidiphila DSM 44928 ACU73867.1 C7Q3D1  
 Caci_6252   Catenulispora acidiphila DSM 44928 ACU75106.1 C7QK32  
 Caci_6874   Catenulispora acidiphila DSM 44928 ACU75713.1 C7Q3E8  
 Caci_6974   Catenulispora acidiphila DSM 44928 ACU75804.1 C7Q3N9  
 Caci_7223   Catenulispora acidiphila DSM 44928 ACU76052.1 C7Q739  
 Cseg_1839   Caulobacter segnis ATCC 21756 ADG10312.1 D5VJA3  
 Cseg_2011   Caulobacter segnis ATCC 21756 ADG10480.1 D5VGN7  
 B7G68_10310   Caulobacter segnis TK0059 AVQ02206.1    
 B7G68_09440   Caulobacter segnis TK0059 AVQ02046.1    
 Caul_0313   Caulobacter sp. K31 ABZ69450.1 B0T4F0  
 Caul_2129   Caulobacter sp. K31 ABZ71257.1 B0T7R9  
 Caul_2412   Caulobacter sp. K31 ABZ71539.1 B0SVS1  
 Celf_0438   Cellulomonas fimi ATCC 484 AEE44581.1    
 Celf_3623   Cellulomonas fimi ATCC 484 AEE47731.1    
 Cfla_0456   Cellulomonas flavigena DSM 20109 ADG73371.1 D5UHU7  
 Cfla_2742   Cellulomonas flavigena DSM 20109 ADG75628.1 D5UJJ0  
 Celgi_2554   Cellulomonas gilvus ATCC 13127 AEI13053.1    
 CBP52_12330   Cellulomonas sp. PSBB021 ASR55751.1    
 A5M85_12410   Cellulophaga lytica DAU203 APU11057.1    
 CJA_0246 (Aga27A)   Cellvibrio japonicus Ueda107 ACE85287.1 B3PGJ1  
 α-galactosidase 27A (Aga27A) 3.2.1.22 Cellvibrio mixtus ATCC 12120 AAS19696.1 Q6QT41  
 CBR65_18810   Cellvibrio sp. PSBB006 ARU29319.1    
 CBR65_15165   Cellvibrio sp. PSBB006 ARU28677.1    
 B0D95_16685   Cellvibrio sp. PSBB023 AQT61564.1    
 β-L-arabinopyranosidase (CpArap27;Cpin_5740) 3.2.1.88 Chitinophaga pinensis DSM 2588 ACU63161.1 C7PCU7  
 Cabther_B0051   Chloracidobacterium thermophilum B AEP13059.1    
 CCALI_01466   Chthonomonas calidirosea T49 CCW35282.1    
 CCALI_01571   Chthonomonas calidirosea T49 CCW35387.1    
 AES38_01010   Clavibacter capsici PF008 ALD14007.1    
 α-galactosidase (Ccel_1237) (Gal27A) 3.2.1.22 [Clostridium] cellulolyticum H10 ACL75593.1
ABG76969.1
B8I0L8  
 Ccel_1597 (P50)   [Clostridium] cellulolyticum H10 ACL75949.1 B8I2F6  
 Clocel_3640   Clostridium cellulovorans 743B ADL53311.1 D9SWM9  
 A7L45_08695   Clostridium estertheticum subsp. estertheticum DSM 8809 APC40140.1    
 α-galactosidase (AgaA) (Aga27A) 3.2.1.22 Clostridium josui FERM P-9684 BAB83765.1 Q8VV86  
 Cspa_c41060   Clostridium saccharoperbutylacetonicum N1-4(HMT) AGF57859.1    
 CLSAP_38570 (AgaA)   Clostridium saccharoperbutylacetonicum N1-504 AQR96533.1    
 Clo1100_0192   Clostridium sp. BNL1100 AEY64483.1    
 Clo1100_0917   Clostridium sp. BNL1100 AEY65174.1    
 Clo1100_1931   Clostridium sp. BNL1100 AEY66126.1    
 CSTERLE_08105   [Clostridium] stercorarium subsp. leptospartum DSM 9219 ANX01536.1    
 Cst_c16870   [Clostridium] stercorarium subsp. stercorarium DSM 8532 AGI39682.1
AGC68671.1
   
 CSTERTH_08205   [Clostridium] stercorarium subsp. thermolacticum DSM 2910 ANW99008.1    
 CPter291_4451   Collimonas pratensis Ter291 AMP16677.1    
 CPter91_4517   Collimonas pratensis Ter91 AMP06824.1    
 A9D14_04555   Croceicoccus marinus E4A9 ARU15583.1    
 A9D14_16550   Croceicoccus marinus E4A9 ARU17926.1    
 B7495_01920   Cryobacterium sp. LW097 ASD23813.1    
 Deipe_0778   Deinococcus peraridilitoris DSM 19664 AFZ66354.1    
 AAM4_2286   Desulfitobacterium hafniense CED92118.1    
 FH5T_07615   Draconibacterium orientale FH5 AHW59502.1    
 HY57_08620   Dyella japonica A8 AIF47330.1    
 HY57_08625   Dyella japonica A8 AIF47331.1    
 CH75_13760   Dyella jiangningensis SBZ 3-12 AHX14143.1    
 ATSB10_27610   Dyella thiooxydans ATSB10 AND70215.1    
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