Activities in Family	Mixed-linkage xylan-specific endo-β-1,4-xylanase (cleaves glycosidic bond after β-1,3-linkage) (EC 3.2.1.-); Exo-β-1,4-mannobiohydrolase / mannobiose-producing exo-β-mannanase (EC 3.2.1.100); Endo-β-1,3-xylanase (EC 3.2.1.32); Endo-β-1,4-glucanase (EC 3.2.1.4); Lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); Endo-β-1,4-mannanase (EC 3.2.1.78);
Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	formerly known as cellulase family I.
External resources	CAZypedia; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (5261); Uniprot accession (85); PDB accession (44); 3D entries (21); cryst (2)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
endo-β-1,4-glucanase / β-1,3:1,4-glucanase H (CelH) (Lic26A;Cel5E)	3.2.1.73	Acetivibrio thermocellus NCIB 10682	AAA23225.1	H6SHY4 P16218	2BV9[A] 1.50 2BVD[A] β-D-Glcp-(1-1)-<non_carb> 1.6 2CIP[A] β-D-Glcp-(1-3)-β-D-Glcp-(1-1)-<non_carb> 1.40 2CIT[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-α-D-Glcp2F-(1-4)-GLU 1.40 2V3G[A] β-D-Glcp-(1-1)-<non_carb> <carb_like_NOY> 1.20 2VI0[A] β-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp4SH-(1-4)-β-D-Glcp-(1-1)-methyl 1.51 6ZPL[C] 3.95
mixed-linkage xylan-specific endo-β-1,4-xylanase (AlXyn26A; M4I23_16475) (Xyn26A)	3.2.1.-	Algibacter sp. L4_22	MCL5130009.1		7XJR[A] 1.85 7XS3[A,B] β-D-Xylp-(1-3)-β-D-Xylp-(1-4)-β-D-Xylp 1.90
endo-β-1,4-mannanase (ManB;YdhT;BLi00735;BL00606)	3.2.1.78	Bacillus licheniformis DSM 13 = ATCC 14580	AAU39670.1 AAU22320.1 AAU22321.2	Q65MP4	cryst
β-mannanase 26A (AmA) (alkaline) (Man26A)	3.2.1.78	Bacillus sp. JAMB750	BAE80444.1	Q2ACI1	4YN5[A] 1.80
β-mannanase 26A (BsMan26A; YdhT; BSU05880) (Man26A)	3.2.1.78	Bacillus subtilis subsp. subtilis str. 168	BAA19712.1 AIY91875.1 AQR80540.1 AQR84753.1 CAB12407.1 CAB12407.2 NP_388469.1	A0A6M3Z7Z2 O05512	2WHK[A] 1.70 3CBW[A,B] 1.27
β-1,4-mannanase (Man; BcMan)	3.2.1.78	Bacillus subtilis Z-2	AAV84100.1	Q5PSP8	2QHA[A,B] 1.45
exo-β-1,4-mannanase 26A (Mana_1;BoMan26A;Bovatus_02927;BACOVA_02092) (periplasmic) (Man26A)	3.2.1.100	Bacteroides ovatus ATCC 8483	ALJ47536.1	A7LW88	4ZXO[A] 1.50
β-1,4-mannanase 26B (Mana_2;BoMan26B;Bovatus_02928;BACOVA_02093) (Man26B)	3.2.1.78 3.2.1.100	Bacteroides ovatus ATCC 8483	ALJ47537.1	A7LW89	6HF2[A] 1.69 6HF4[A] α-D-Galp-(1-6)[β-D-Manp-(1-4)]-β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp 1.78
β-mannanase (Man26A)	3.2.1.78	Cellulomonas fimi ATCC 484	AAD42774.1	Q9XCV5	2BVT[A,B] β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp 2.9 2BVY[A] 2.25 2X2Y[A,B] 2.35 3TP4[A,B] 1.98
β-1,4-mannanase 26A (ManA; CjMan26A; CJA_2770; eMAN; E-BMACJ) (Man26A)	3.2.1.78	Cellvibrio japonicus Ueda107	ACE82849.1 CAA57670.2	B3PBK3 P49424	1GVY[A] β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp2F-(1-1)-<non_carb> 1.70 1GW1[A] β-D-Manp-(1-4)-β-D-Manp-(1-4)-α-D-Manp2F-(1-4)-GLU 1.65 1J9Y[A] 1.85 1ODZ[A,B] β-D-Manp-(1-4)-α-D-Manp 1.4 1R7O[A] 1.85 2WHM[A] β-D-Manp-(1-4)-α-D-Manp 1.50
mannobiose-producing exo-β-mannanase (CJA_0236;CjMan26C) (Man26C)	3.2.1.100	Cellvibrio japonicus Ueda107	ACE84009.1	B3PGI1	2VX4[A] 1.70 2VX5[A] β-D-Manp 1.47 2VX6[A] α-D-Galp-(1-6)[β-D-Manp-(1-4)]-β-D-Manp-(1-4)-β-D-Manp-(1-4)-β-D-Manp 1.57 2VX7[A] β-D-Manp-(1-4)-β-D-Manp 1.80 4CD4[A] β-D-Manp-(1-1)-<non_carb> <carb_like_IFM> 1.20 4CD5[A] β-D-Manp <carb_like_MVL> 1.10
β-mannanase (KMAN; KMAN-3)	3.2.1.78	Klebsiella oxytoca KUB-CW2-3	AIT71003.1		7EET[A] 2.57
[diatom β-mannan] β-1,4-mannanase 26A (GH26A; FG28_RS02340; FG28DRAFT_0469)	3.2.1.78	Muricauda sp. MAR_2010_75	WP_036379595.1		6Q75[A,B] 1.75
endo-β-1,4-mannanase 26C (GH26C; FG28DRAFT_0463)	3.2.1.78	Muricauda sp. MAR_2010_75	WP_036379585.1		6Q78[A,B] 1.50
mixed-linkage β-glucanase/xyloglucanase (Orf4; CMCase; PbrCel5A; PBR_0368) (Cel5A)		Segatella bryantii B14	AAC97596.1 AAA22909.1 AAB20175.1	D8DY19 O06842 Q44878 Q53324	6D2W[A,B] 2.10 6D2X[A] 1.72
endo-β-1,3-xylanase (XylC; TnXyn26A; CTN_0617) (Xyn26A)	3.2.1.32	Thermotoga neapolitana DSM 4359	ACM22793.1 AAB70868.1	B9K760 O33841	cryst
β-1,3-xylanase (Xyl4)	3.2.1.32	Vibrio sp. AX-4	BAD51934.1	D5MP61	2DDX[A] 0.86 3VPL[A] β-D-Xylp-(1-3)-β-D-Xylp-(1-3)-α-D-Xylp2F-(1-4)-GLU β-D-Xylp-(1-4)-β-D-Xylp-(1-3)-β-D-Xylp2F-(1-1)-<non_carb> 1.20
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
endo-β-1,4-mannanase (PaMan26A;Pa_5_1950) (Man26A)	3.2.1.78	Podospora anserina S mat+	CAP61906.1 ADO14135.1 CDP28981.1	B2AEP0 E2GHW2	3ZM8[A] 2.85
β-mannanase (ManC; RsManC; RsMan26C) (Man26C)	3.2.1.78	Symbiotic protist of Reticulitermes speratus	BAL68133.1	H7CGE2	3WDQ[A] 1.30 3WDR[A] β-D-Glcp-(1-4)-α-D-Manp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Manp 1.40
endo-β-1,4-mannanase 26A (YpenMan26A)	3.2.1.78	Yunnania penicillata Man26A	AYU65281.1	A0A3G4RHU4	6HPF[A] α-D-Galp-(1-6)[β-D-Manp-(1-4)]-β-D-Manp-(1-4)[α-D-Galp-(1-6)]-α-D-Manp 1.36
unclassified
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
endo-mannanase		metagenome			6UEH[A] 1.85
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