Known Activities	β-glucosidase (EC 3.2.1.21); β-galactosidase (EC 3.2.1.23); β-mannosidase (EC 3.2.1.25); β-glucuronidase (EC 3.2.1.31); β-D-fucosidase (EC 3.2.1.38); phlorizin hydrolase (EC 3.2.1.62); exo-β-1,4-glucanase (EC 3.2.1.74); 6-phospho-β-galactosidase (EC 3.2.1.85); 6-phospho-β-glucosidase (EC 3.2.1.86); strictosidine β-glucosidase (EC 3.2.1.105); lactase (EC 3.2.1.108); amygdalin β-glucosidase (EC 3.2.1.117); prunasin β-glucosidase (EC 3.2.1.118); raucaffricine β-glucosidase (EC 3.2.1.125); thioglucosidase (EC 3.2.1.147); β-primeverosidase (EC 3.2.1.149); isoflavonoid 7-O-β-apiosyl-β-glucosidase (EC 3.2.1.161); hydroxyisourate hydrolase (EC 3.-.-.-); β-glycosidase (EC 3.2.1.-)
Mechanism	Retaining
Clan	GH-A
3D Structure Status	( β / α ) 8
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental); absent in plant myrosinases
External resources	CAZypedia; HOMSTRAD; InterPro; PFAM; PRINTS; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (3805); Uniprot accession (1211); PDB accession (117); 3D entries (29); cryst (2)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
ASAC_1390		Acidilobus saccharovorans 345-15	ADL19795.1
Aboo_0529		Aciduliprofundum boonei T469	ADD08340.1
Cmaq_0408		Caldivirga maquilingensis IC-167	ABW01253.1	A8MBR0
Cmaq_0652		Caldivirga maquilingensis IC-167	ABW01492.1	A8MCI6
Cmaq_1537		Caldivirga maquilingensis IC-167	ABW02360.1	A8M9E1
DKAM_0253 (fragment)		Desulfurococcus kamchatkensis 1221n	ACL10579.1	B8D332
Sequence 15 from patent US 6368844		Desulfurococcus sp.	CAH68683.1 CAT81435.1
PTO1259		Picrophilus torridus DSM 9790	AAT43844.1
PTO1453		Picrophilus torridus DSM 9790	AAT44038.1
PAE1736 (possible fragment)		Pyrobaculum aerophilum str. IM2	AAL63693.1 NP_559511.1	Q8ZWK9
Pars_0741		Pyrobaculum arsenaticum DSM 13514	ABP50327.1	A4WIW0
Pcal_0630		Pyrobaculum calidifontis JCM 11548	ABO08056.1	A3MTT9
Pisl_1708		Pyrobaculum islandicum DSM 4184	ABL88859.1	A1RV77
PAB1740		Pyrococcus abyssi GE5	CAB49848.1 NP_126617.1	Q9V058
PAB2376 (Bgal)		Pyrococcus abyssi GE5	CAB50447.1 NP_127217.1	Q9UYG5
β-mannosidase (BmnA;PF1208) (Man1)	3.2.1.25	Pyrococcus furiosus DSM 3638	AAL81332.1 ABC06395.1 NP_578937.1
β-glucosidase (CelB;BGLPf;PF0073;Pf-β-gly) (Cel1A)	3.2.1.21	Pyrococcus furiosus DSM 3638	AAC25555.1 AAL80197.1 ABC06396.1 NP_577802.1	Q51723
β-glucosidase (BglA;PF0442) (Cel1B)	3.2.1.21	Pyrococcus furiosus DSM 3638	AAG28457.1 AAL80566.1 NP_578171.1	Q9HHB3
PF0356		Pyrococcus furiosus DSM 3638	AAL80480.1 NP_578085.1	Q8U3U9
β-mannosidase (BmnA)	3.2.1.25	Pyrococcus furiosus DSM 3638 VC1	AAC44387.1 AAM56789.1 CAH68690.1 CAT81449.1	Q51733
alkyl β-glucosidase (membrane-bound) (BGPh;PH0366)	3.2.1.21	Pyrococcus horikoshii OT3	ABC06393.1 BAA29440.1 NP_142340.1	O58104	1VFF[A]
β-mannosidase (BglB;PH0501)	3.2.1.25	Pyrococcus horikoshii OT3	ABC06394.1 BAA29589.1 NP_142473.1	O58237
β-galactosidase	3.2.1.23	Pyrococcus woesei	AAB97862.1	O52629.1
Shell_1057		Staphylothermus hellenicus DSM 12710	ADI32161.1
Shell_0804		Staphylothermus hellenicus DSM 12710	ADI31917.1
Smar_1391		Staphylothermus marinus F1	AAM56784.1 ABN70482.1 CAH68685.1 CAT81439.1	A3DPC2
β-glycosidase (BgaS;Saci_1849)	3.2.1.-	Sulfolobus acidocaldarius DSM 639	AAY81155.1 YP_256448.1
LD85_2633		Sulfolobus islandicus L.D.8.5	ADB88250.1
LS215_2510		Sulfolobus islandicus L.S.2.15	ACP36466.1
M1425_2338		Sulfolobus islandicus M.14.25	ACP39066.1
M1627_2417		Sulfolobus islandicus M.16.27	ACP56269.1
M164_2345		Sulfolobus islandicus M.16.4	ACR42944.1
LacS		Sulfolobus islandicus REN1H1	ABY78030.1	B1P2F7
YG5714_2480		Sulfolobus islandicus Y.G.57.14	ACP46718.1
YN1551_0427		Sulfolobus islandicus Y.N.15.51	ACP47593.1
β-glycosidase (Bgly;SSG)	3.2.1.-	Sulfolobus shibatae	AAA79030.1	P50388
β-glycosidase (LacS)	3.2.1.21 3.2.1.23	Sulfolobus solfataricus 98/2	AAD21094.1
β-galactosidase	3.2.1.23	Sulfolobus solfataricus DSM1616	CAA34074.1	P14288.2
β-glycosidase S (LacS;Ss-β-gly;SSO3019)	3.2.1.- 3.2.1.21 3.2.1.23	Sulfolobus solfataricus P2	AAA72843.1 AAE29896.1 AAK43121.1 ABC06397.1 ACW34039.1 CAC24042.1 CAJ18790.1 NP_344331.1	P22498.2	1GOW[A,B] 1UWI[A,B,C,D] 1UWQ[A,B] 1UWR[A,B] 1UWS[A,B] 1UWT[A,B] 1UWU[A,B] 2CEQ[A,B] 2CER[A,B]
ST0773		Sulfolobus tokodaii str. 7	BAB65785.1 NP_376676.1	Q973X5
Sequence 20 from patent US 6368844		Thermococcus alcaliphilus	AAM56787.1 CAH68688.1 CAT81445.1
β-mannanase (63GB1)		Thermococcus chitonophagus	AAM56793.1 ACM94264.1 CAH68694.1 CAT81457.1
Sequence 21 from patent US 6368844		Thermococcus chitonophagus	AAM56788.1 CAH68689.1 CAT81447.1
β-glycosidase (TK1761)	3.2.1.-	Thermococcus kodakarensis KOD1	BAA78713.1 BAD85950.1 CAT70557.1	Q9YGB8
β-glycosidase (TK1827)	3.2.1.-	Thermococcus kodakarensis KOD1	BAD86016.1 CAT70991.1	Q5JDV2
TSIB_0324		Thermococcus sibiricus MM 739	ACS89390.1
TSIB_0334		Thermococcus sibiricus MM 739	ACS89400.1
TSIB_0580		Thermococcus sibiricus MM 739	ACS89645.1
unnamed protein product		Thermococcus sp.	CAH68686.1 CAT81441.1
Sequence 2 from patent US 6713281		Thermococcus sp. 9N2	AAM56785.1 AAT23807.1
β-glucosidase (BglT)	3.2.1.21	Thermococcus sp. AL662	CAA94187.1	O08324
Tpen_1110		Thermofilum pendens Hrk 5	ABL78509.1	A1RZ79
Tpen_1675		Thermofilum pendens Hrk 5	ABL79070.1	A1S0U0
Ta1323		Thermoplasma acidophilum DSM 1728	CAC12444.1 NP_394779.1	Q9HIL6
TVG0691226	3.2.1.23	Thermoplasma volcanium GSS1	BAB59827.1 NP_111204.1	Q97AX4
Tneu_0727		Thermoproteus neutrophilus V24Sta	ACB39666.1	B1YD03
Tagg_1110		Thermosphaera aggregans DSM 11486	ADG91379.1
β-glycosidase (Tα-β-gly)	3.2.1.-	Thermosphaera aggregans M11TL	AAD43138.1 AAM56782.1	Q9YGA8	1QVB[A,B]