Glycoside Hydrolase Family 16

Activities in Familyxyloglucan:xyloglucosyltransferase (EC 2.4.1.207); keratan-sulfate endo-1,4-β-galactosidase (EC 3.2.1.103); endo-1,3-β-glucanase / laminarinase (EC 3.2.1.39); endo-1,3(4)-β-glucanase (EC 3.2.1.6); licheninase (EC 3.2.1.73); β-agarase (EC 3.2.1.81); κ-carrageenase (EC 3.2.1.83); xyloglucanase (EC 3.2.1.151); endo-β-1,3-galactanase (EC 3.2.1.181); [retaining] β-porphyranase (EC 3.2.1.178); hyaluronidase (EC 3.2.1.35); endo-β-1,4-galactosidase (EC 3.2.1.-); chitin β-1,6-glucanosyltransferase (EC 2.4.1.-); β-transglycosidase (EC 2.4.1.-); β-glycosidase (EC 3.2.1.-); β-carrageenase (EC 3.2.1.-)
Mechanism Retaining
ClanGH-B
3D Structure Statusβ-jelly roll
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteMany members have been assigned to subfamilies as described by Viborg et al. (2019) J Biol Chem. 2019 Sep 9 (PMID: 31501245).
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE; XTH-World;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (12736); Uniprot accession (1506); PDB accession (109); 3D entries (53); cryst (1)
All (11470) Archaea (27) Bacteria (7305) Eukaryota (4051) Viruses (62) unclassified (25) Structure (53 - 1 cryst) Characterized (229)
unclassified
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 β-glucanase precursor   feces metagenome SJX74186.1     3
 UFOVP1247_35   freshwater metagenome CAB4193193.1    
 UFOVP970_75   freshwater metagenome CAB4174734.1    
 lichenase (Licbm2)   Transformation vector pBISN1-IN ACI16381.1     21
 β-agarase, partial   uncultured microorganism ANT83229.1     16
 β-glucanase (G05-26) 3.2.1.- uncultured organism ACY24737.1 D8VMV3   25
 endo-β-1,3(4)-glucanase (G05-27) 3.2.1.73 uncultured organism ACY24738.1 D8VMV4   25
 β-glucanase 3.2.1.- uncultured organism ADD61790.1 D9ZEN6   21
 ORF   uncultured organism ADD61857.1 D9ZEV3   3
 ORF   uncultured organism ADD61979.1 D9ZF75   3
 ORF   uncultured organism AGU11906.1    
 ORF (fragment)   uncultured organism AGU11270.1    
 Sequence 30041 from patent US 7504490.   unidentified ACP97823.1     18
 Sequence 1 from patent US 6541233   unidentified AAP61550.1     21
 Sequence 2 from patent US 7541162.   unidentified ACS10738.1     3
 LMG31885_29920   unidentified CAD7739295.1
CAD7739298.1
    3
 JN350_04000   unidentified QSB24733.1     3
 JN350_15435   unidentified QSB22785.1    
 JN350_15115   unidentified QSB22738.1    
 JN350_16780   unidentified QSB22978.1    
 JN350_18305   unidentified QSB23214.1    
 JN350_14590   unidentified QSB22660.1    
 LMG31886_30480   unidentified GBBC 2199 CAD7740169.1     3
 MM171B00931_0002   viral metagenome QJB03038.1    
 MM171A01442_0002   viral metagenome QJA98926.1    
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