Glycoside Hydrolase Family 16

Activities in Familyxyloglucan:xyloglucosyltransferase (EC 2.4.1.207); keratan-sulfate endo-1,4-β-galactosidase (EC 3.2.1.103); endo-1,3-β-glucanase / laminarinase (EC 3.2.1.39); endo-1,3(4)-β-glucanase (EC 3.2.1.6); licheninase (EC 3.2.1.73); β-agarase (EC 3.2.1.81); κ-carrageenase (EC 3.2.1.83); xyloglucanase (EC 3.2.1.151); endo-β-1,3-galactanase (EC 3.2.1.181); [retaining] β-porphyranase (EC 3.2.1.178); hyaluronidase (EC 3.2.1.35); endo-β-1,4-galactosidase (EC 3.2.1.-); chitin β-1,6-glucanosyltransferase (EC 2.4.1.-); β-transglycosidase (EC 2.4.1.-); β-glycosidase (EC 3.2.1.-); endo-β-1,3-galactanase (EC 3.2.1.181); β-carrageenase (EC 3.2.1.-)
Mechanism Retaining
ClanGH-B
3D Structure Statusβ-jelly roll
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteMany members have been assigned to subfamilies as described by Viborg et al. (2019) J Biol Chem. 2019 Sep 9 (PMID: 31501245).
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE; XTH-World;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (10528); Uniprot accession (1506); PDB accession (96); 3D entries (48); cryst (1)
All (9282) Archaea (26) Bacteria (5792) Eukaryota (3373) Viruses (54) unclassified (37) Structure (48 - 1 cryst) Characterized (214)
unclassified
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 β-glucanase precursor   feces metagenome SJX74186.1     3
 lichenase (Licbm2)   Transformation vector pBISN1-IN ACI16381.1     21
 β-agarase, partial   uncultured microorganism ANT83229.1     16
 β-glucanase (G05-26) 3.2.1.- uncultured organism ACY24737.1 D8VMV3   25
 endo-β-1,3(4)-glucanase (G05-27) 3.2.1.73 uncultured organism ACY24738.1 D8VMV4   25
 β-glucanase 3.2.1.- uncultured organism ADD61790.1 D9ZEN6   21
 ORF   uncultured organism ADD61857.1 D9ZEV3   3
 ORF   uncultured organism ADD61979.1 D9ZF75   3
 ORF   uncultured organism AGU11906.1    
 ORF (fragment)   uncultured organism AGU11270.1    
 Sequence 30041 from patent US 7504490.   unidentified ACP97823.1     18
 Sequence 1 from patent US 6541233   unidentified AAP61550.1     21
 Sequence 2 from patent US 7541162.   unidentified ACS10738.1     3
 GWR56_11710   unidentified QHS56170.1     3
 GWR55_09970   unidentified QHS52030.1     3
 GXP71_02835   unidentified QHT55131.1     24
 GXP71_00260   unidentified QHT54687.1     3
 GXP71_16885   unidentified QHT57583.1     3
 GXP71_16740   unidentified QHT57556.1     21
 GXP67_19995   unidentified QHT68766.1    
 GXP67_35905   unidentified QHT71674.1     3
 GXP70_14910   unidentified QHT61115.1    
 GXP70_14795   unidentified QHT61096.1    
 GXP70_24495   unidentified QHT62826.1    
 GXP70_16600   unidentified QHT61421.1    
 GXP70_14390   unidentified QHT61022.1     3
 GXP70_14290   unidentified QHT61003.1    
 GXP70_24460   unidentified QHT62820.1    
 GWR21_30630   unidentified QHS63780.1     3
 GWR21_30640   unidentified QHS63782.1     3
 GWR21_26225   unidentified QHS62954.1     3
 GWR21_12950   unidentified QHS60466.1     3
 GWR21_02960   unidentified QHS58591.1     10
 GWK76_02530   unidentified QHU92560.1    
 GWK74_02600   unidentified QHU90844.1    
 GWK78_02480   unidentified QHU94267.1    
 GWK77_02665   unidentified QHU93419.1    
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