Glycoside Hydrolase Family 16 / Subf 20

Activities in Sub Family	Xyloglucan:xyloglucosyl transferase (EC 2.4.1.207); Xyloglucan-specific endo-β-1,4-glucanase / endo-xyloglucanase (EC 3.2.1.151);
Mechanism	Retaining e
Clan	GH-B
3D Structure Status	β-jelly roll
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Many members have been assigned to subfamilies as described by Viborg et al. (2019) J Biol Chem. 2019 Sep 9 (PMID: 31501245).
External resources	CAZypedia; HOMSTRAD; PRINTS; PROSITE; XTH-World;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (20); Uniprot accession (33); PDB accession (6); 3D entries (2); cryst (0)

Eukaryota

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

Subf

xyloglucan endotransglycosylase 16A (PttXET16-34;XTH16-34) (Xet16A)

AAN87142.1
CBD24810.1

1UMZ[A,B]	β-D-Galp-(1-2)-α-D-Xylp-(1-6)[α-D-Xylp-(1-6)-β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-D-1-deoxy-Glcp	1.8
1UN1[A,B]		2.1

xyloglucanase (NXG1;NXET) (formerly xyloglucan endotransferase 1)

2UWA[A,B,C]		1.8
2UWB[A,B]		2.0
2UWC[A,B]		2.30
2VH9[A,B]	β-D-Galp-(1-2)-α-D-Xylp-(1-6)[α-D-Xylp-(1-6)-β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[β-D-Galp-(1-2)-α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU	2.1