Glycoside Hydrolase Family 16 / Subf 1

Activities in Sub Family	[retaining] β-1,3-glucanosyltransglucosidase (EC 2.4.1.-); Hyaluronidase / hyaluronoglucosaminidase (EC 3.2.1.35); Endo-β-1,3-glucanase / laminarinase (EC 3.2.1.39); Endo-β-1,3(4)-glucanase / lichenase-laminarinase (EC 3.2.1.6); Lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73);
Mechanism	Retaining e
Clan	GH-B
3D Structure Status	β-jelly roll
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Many members have been assigned to subfamilies as described by Viborg et al. (2019) J Biol Chem. 2019 Sep 9 (PMID: 31501245).
External resources	CAZypedia; HOMSTRAD; PRINTS; PROSITE; XTH-World;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (5539); Uniprot accession (16); PDB accession (12); 3D entries (3); cryst (0)

Eukaryota

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

Subf

exo-β-1,3-glucosyltransferase / elongating β-transglucosylase (Bgt16A;PtBgt16A)

1.59

β-1,3-1,4-glucanase (PtLic16A) (Lic16A)

3WDT[A,B,C,D]		1.98
3WDU[A,B,C,D]	β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp	2.25
3WDV[A,B,C,D]	β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp	1.94
3WDW[A,B]		1.80
3WDX[A,B]	β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-α-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp	1.90
3WDY[A,B,C,D]	β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp	1.94

β-1,3-1,6- / β-1,3-1,4-glucan hydrolase (laminarinase) (Lam16A)

2CL2[A]		1.35
2W39[A]	β-D-Glcp-(1-3)-β-D-Glcp	1.10
2W52[A]	β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp β-D-Glcp-(1-6)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp	1.56
2WLQ[A]	β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp	1.40
2WNE[A]	β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-α-D-Glcp	2.12