Glycoside Hydrolase Family 145

Activities in FamilyL-Rhα-α-1,4-GlcA-specific α-L-rhamnosidase (EC 3.2.1.-)
Mechanism Retaining
3D Structure Statusseven-bladed β-propeller
Catalytic Nucleophile/BaseHis
NoteCreated after Munoz-Munoz et al., Proc. Natl. Acad. Sci. USA (2017) (PMID=28396425); these enzymes hydrolyze a-L-rhamnose residues linked to position 4 of a glucuronic acid using an unusual catalytic machinery comprising a histidine residue. The active site of these a-L-rhamnosidases is located on the opposite side of CAZymes with similar b-propeller folds. Surprisingly, the "normal" side of the b-propeller bears the highest residue conservation in the family and may well have another function. The latter is unknown but strong ressemblance to family PL25 suggests a polysaccharide lyase activity.
Statistics GenBank accession (99); Uniprot accession (1); PDB accession (5); 3D entries (4); cryst (0)
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3D
 BofuT4_P044640.1   Botrytis cinerea T4 CCD45446.1    
 SAMEA4029010_CIC11G00000001359   [Candida] intermedia CBS 141442 SGZ47226.1    
 SAMEA4029009_CIC11G00000000585   [Candida] intermedia PYCC 4715 SGZ49612.1    
 CANTEDRAFT_94676   Candida tenuis ATCC 10573 EGV61793.1    
 FFUJ_14848   Fusarium fujikuroi IMI 58289 CCT67810.1    
 FGRAMPH1_01T21617   Fusarium graminearum PH-1 SCB65023.1    
 MGG_14899   Magnaporthe oryzae 70-15 EHA47224.1    
 UCRNP2_6875 (fragment)   Neofusicoccum parvum UCRNP2 EOD46375.1    
 NCU00990   Neurospora crassa OR74A EAA34664.3    
 Putative protein of unknown function (fragment)   Podospora anserina S mat+ CDP22308.1
CAP59667.1
   
 SAPIO_CDS0694   Scedosporium apiospermum IHEM 14462 KEZ46379.1    
 SCHCODRAFT_232948   Schizophyllum commune H4-8 EFJ01384.1    
 SERLA73DRAFT_161488   Serpula lacrymans var. lacrymans S7.3 EGN97454.1    
 SETTUDRAFT_33355   Setosphaeria turcica Et28A EOA84368.1    
 M422DRAFT_185901   Sphaerobolus stellatus SS14 KIJ31449.1    
 MYCTH_2307864   Thermothelomyces thermophila ATCC 42464 AEO59504.1    
 THITE_2120556   Thielavia terrestris NRRL 8126 AEO69808.1    
 BN946_scf185016.g41   Trametes cinnabarina BRFM137 CDO73884.1    
 TRAVEDRAFT_174832   Trametes versicolor FP-101664 SS1 EIW52757.1    
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