Glycoside Hydrolase Family 145

Activities in FamilyL-Rhα-α-1,4-GlcA α-L-rhamnohydrolase (EC 3.2.1.-)
Mechanism Retaining
3D Structure Statusseven-bladed β-propeller
Catalytic Nucleophile/BaseHis
NoteCreated after Munoz-Munoz et al., Proc. Natl. Acad. Sci. USA (2017) (PMID=28396425); these enzymes hydrolyze a-L-rhamnose residues linked to position 4 of a glucuronic acid using an unusual catalytic machinery comprising a histidine residue. The active site of these a-L-rhamnosidases is located on the opposite side of CAZymes with similar b-propeller folds. Surprisingly, the "normal" side of the b-propeller bears the highest residue conservation in the family and may well have another function. The latter is unknown but strong ressemblance to family PL25 suggests a polysaccharide lyase activity.
External resourcesCAZypedia;
Statistics GenBank accession (251); Uniprot accession (1); PDB accession (5); 3D entries (4); cryst (0)
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Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D
 EPY81_12305   Acinetobacter pittii NQ-003 QHQ32130.1    
 EPY81_16305   Acinetobacter pittii NQ-003 QHQ32840.1    
 TL08_16695   Actinoalloteichus hymeniacidonis HPA177(T) (=DSM 45092(T)) AOS64139.1    
 SAMN04489716_2891   Actinoplanes derwentensis DSM 43941 SDT21880.1    
 AFR_29060   Actinoplanes friuliensis DSM 7358 AGZ44073.1    
 Amir_4945   Actinosynnema mirum DSM 43827 ACU38771.1    
 APASM_5003   Actinosynnema pretiosum subsp. pretiosum ATCC 31280 AXX32368.1    
 CNX65_24385   Actinosynnema pretiosum X47 ATE56025.1    
 neuraminidase   Alkalitalea saponilacus DSM 24412 ASB47862.1    
 AMEC673_16885   Alteromonas macleodii str. English Channel 673 AFT76059.1    
 FJN15_15590   Alteromonas mediterranea AltCH17 QGX63106.1    
 FJN15_15290   Alteromonas mediterranea AltCH17 QGX63053.1    
 ALT761_04129   Alteromonas sp. 76-1 VEM00340.1    
 ALT761_04175   Alteromonas sp. 76-1 VEM00388.1    
 AMED_7434   Amycolatopsis mediterranei U32 ADJ49148.1    
 YIM_23735   Amycolatopsis sp. YIM 10 QFU89924.1    
 STSP2_00746   Anaerohalosphaera lusitana ST-NAGAB-D1 AQT67598.1    
 neuraminidase   Arachidicoccus sp. KIS59-12 AYD48252.1    
 Astex_3547   Asticcacaulis excentricus CB 48 ADU15177.1    
 EM6_3353   Asticcacaulis excentricus M6 BBF82712.1    
 CGC64_05340   Bacteroides caccae ATCC 43185 ASM67811.1    
 neuraminidase   Bacteroides caecimuris I48 ANU56237.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (BACCELL_00856) 3.2.1.- Bacteroides cellulosilyticus DSM 14838 EEF91490.1   5MVH[A]
 BcellWH2_01654   Bacteroides cellulosilyticus WH2 ALJ58907.1    
 ABI39_02695   Bacteroides dorei CL03T12C01 AND18477.1    
 neuraminidase   Bacteroides dorei isolate HS1_L1_B_010 AII65648.1    
 GV66_02770   Bacteroides dorei isolate HS1_L3_B_079 AII66702.1    
 IY41_02640   Bacteroides dorei isolate HS2_L_2_B_045b ALA72499.1    
 neuraminidase   Bacteroides heparinolyticus F0111 AVM58295.1    
 neuraminidase   Bacteroides intestinalis APC919/174 QDO70678.1    
 neuraminidase   Bacteroides intestinalis APC919/174 QDO67478.1    
 BACINT_00347   Bacteroides intestinalis DSM 17393     5MUM[A]
 neuraminidase   Bacteroides ovatus 3725 D1 iv QDM11160.1    
 Bovatus_03783 / BACOVA_03493   Bacteroides ovatus ATCC 8483 ALJ48388.1   4IRT[A]
 neuraminidase   Bacteroides ovatus FDAARGOS_733 QGT73112.1    
 BACOV975_01572   Bacteroides ovatus V975 SCV07798.1    
 Bacsa_1608   Bacteroides salanitronis DSM 18170 ADY36173.1    
 Btheta7330_04610   Bacteroides thetaiotaomicron 7330 ALJ44126.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase / exo-acting α-L-rhamnosidase (BT3686;BT_3686) 3.2.1.- Bacteroides thetaiotaomicron VPI-5482 AAO78791.1   5MUK[A]
5MUL[A]
 Bun01g_00430   Bacteroides uniformis NBRC 113350 BBK85673.1    
 BvMPK_4254   Bacteroides vulgatus mpk ALK86796.1    
 VIC01_00644   Bacteroides vulgatus VIC01 QEW35176.1    
 neuraminidase   Bacteroides xylanisolvens H207 QDH54106.1    
 neuraminidase   Bacteroides zoogleoformans ATCC 33285 AVM53996.1    
 ACH79_15865 (fragment)   Bradyrhizobium sp. CCBAU 051011 QHO73899.1    
 DCN14_18050   Burkholderia sp. IDO3 AXK64598.1    
 WS87_04285   Burkholderia sp. MSMB0856 AOJ85942.1    
 WS87_04295   Burkholderia sp. MSMB0856 AOJ85944.1    
 BSFP_027170   Burkholderia stabilis FERMP-21014 BAX59873.1    
 BSFP_027150   Burkholderia stabilis FERMP-21014 BAX59871.1    
 C2869_06290   Catenovulum sp. CCB-QB4 AWB66072.1    
 E5225_06725   Cellulomonas sp. Z28 QCB93288.1    
 SAMN06298216_2310   Cytophagales bacterium TFI 002 SOE21857.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (HMPREF9455_02360) 3.2.1.- Dysgonomonas gadei ATCC BAA-286 EGK01332.1    
 neuraminidase   Echinicola sp. LN3S3 QDH77890.1    
 DN752_08775   Echinicola strongylocentroti MEBiC08714 AWW30209.1    
 Echvi_0488   Echinicola vietnamensis DSM 17526 AGA76772.1    
 CWS02_23110 (fragment)   Enterobacter sp. EA-1 AUP77012.1    
 CWS02_23105 (fragment)   Enterobacter sp. EA-1 AUP77011.1    
 GN242_16660   Erwinia sp. J780 QGU88756.1    
 FLA_5507   Filimonas lacunae NBRC 104114 BAV09458.1    
 EJO69_05305   Flaviflexus salsibiostraticola KCTC 33148 AZN29787.1    
 neuraminidase   Flavihumibacter sp. SB-02 QGW27552.1    
 neuraminidase   Flavisolibacter ginsenosidimutans Gsoil 636 QEC58411.1    
 neuraminidase   Flavobacterium commune PK15 AOZ98835.1    
 HYN56_19350   Flavobacterium crocinum HYN0056 AWK06268.1    
 neuraminidase   Flavobacterium sp. KBS0721 QDW22445.1    
 neuraminidase   Flavobacterium sp. SLB01 QGK74231.1    
 neuraminidase   Georgenia sp. Z294 QDB80652.1    
 GRFL_2251   Gramella flava JLT2011 APU68975.1    
 neuraminidase   Gramella fulva SH35 AVR47414.1    
 IMCC3135_10240   Granulosicoccus antarcticus IMCC3135 ASJ72142.1    
 YC6258_04555   Gynuella sunshinyii YC6258 AJQ96587.1    
 neuraminidase   Hymenobacter sp. 17J68-5 QDA58744.1    
 SAMN04515669_4177   Jiangella sp. DSM 45060 SDT46315.1    
 GT409_02010   Kiritimatiellaeota bacterium S-5007 QHI68278.1    
 NCTC9668_00292   Klebsiella aerogenes NCTC9668 VEC95070.1    
 DA718_12825   Klebsiella huaxiensis WCHKl090001 QBG08014.1    
 GW952_25070   Klebsiella michiganensis BD177 QHS48653.1    
 CHQ91_02660   Klebsiella michiganensis F107 QHO84869.1    
 CHQ91_11630   Klebsiella michiganensis F107 QHO86455.1    
 CEW81_00155 (fragment)   Kluyvera georgiana YDC799 ASG62411.1    
 GHC38_00095   Kluyvera intermedia HR2 QGH37132.1    
 GHC21_00095   Kluyvera intermedia N2-1 QGH28150.1    
 NCTC12125_03813 (fragment)   Kluyvera intermedia NCTC12125 VDZ85608.1    
 Lbys_2519   Leadbetterella byssophila DSM 17132 ADQ18181.1    
 BBK82_39695   Lentzea guizhouensis DHS C013 ANZ43405.1    
 FE772_18170   Lysobacter enzymogenes YC36 QCW27274.1    
 MFFC18_27830 (fragment)   Mariniblastus fucicola FC18 QEG22895.1    
 MFFC18_27850   Mariniblastus fucicola FC18 QEG22897.1    
 CJ739_3933   Mariniflexile sp. TRM1-10 AXP82991.1    
 EYF70_08645   Massilia albidiflava DSM 17472 QBI00910.1    
 neuraminidase   Massilia flava DSM 26639 QGZ42953.1    
 neuraminidase   Massilia lutea DSM 17473 QBE67449.1    
 DIR46_11140 (neuraminidase)   Massilia timonae CCUG 43427 AWL04923.1    
 neuraminidase   Massilia umbonata DSMZ 26121 QCP10739.1    
 MROS_0979   Melioribacter roseus P3M-2 AFN74220.1    
 F7D14_09020   Methylocystis parvus BRCS2 QGM97586.1    
 CQW49_07015   Methylosinus trichosporium OB3b ATQ67665.1    
 CQW49_23070   Methylosinus trichosporium OB3b ATQ70845.1    
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