Glycoside Hydrolase Family 145

Activities in FamilyL-Rhα-α-1,4-GlcA-specific α-L-rhamnosidase (EC 3.2.1.-)
Mechanism Retaining
3D Structure Statusseven-bladed β-propeller
Catalytic Nucleophile/BaseHis
NoteCreated after Munoz-Munoz et al., Proc. Natl. Acad. Sci. USA (2017) (PMID=28396425); these enzymes hydrolyze a-L-rhamnose residues linked to position 4 of a glucuronic acid using an unusual catalytic machinery comprising a histidine residue. The active site of these a-L-rhamnosidases is located on the opposite side of CAZymes with similar b-propeller folds. Surprisingly, the "normal" side of the b-propeller bears the highest residue conservation in the family and may well have another function. The latter is unknown but strong ressemblance to family PL25 suggests a polysaccharide lyase activity.
Statistics GenBank accession (94); Uniprot accession (1); PDB accession (5); 3D entries (4); cryst (0)
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D
 TL08_16695   Actinoalloteichus hymeniacidonis HPA177(T) (=DSM 45092(T)) AOS64139.1    
 SAMN04489716_2891   Actinoplanes derwentensis DSM 43941 SDT21880.1    
 AFR_29060   Actinoplanes friuliensis DSM 7358 AGZ44073.1    
 Amir_4945   Actinosynnema mirum DSM 43827 ACU38771.1    
 CNX65_24385   Actinosynnema pretiosum X47 ATE56025.1    
 neuraminidase   Alkalitalea saponilacus DSM 24412 ASB47862.1    
 AMEC673_16885   Alteromonas macleodii str. English Channel 673 AFT76059.1    
 AMED_7434   Amycolatopsis mediterranei U32 ADJ49148.1    
 Astex_3547   Asticcacaulis excentricus CB 48 ADU15177.1    
 neuraminidase   Bacteroides caccae ATCC 43185 ASM67811.1    
 neuraminidase   Bacteroides caecimuris I48 ANU56237.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (BACCELL_00856) 3.2.1.- Bacteroides cellulosilyticus DSM 14838 EEF91490.1   5MVH[A]
 BcellWH2_01654   Bacteroides cellulosilyticus WH2 ALJ58907.1    
 ABI39_02695   Bacteroides dorei CL03T12C01 AND18477.1    
 neuraminidase   Bacteroides dorei isolate HS1_L1_B_010 AII65648.1    
 GV66_02770   Bacteroides dorei isolate HS1_L3_B_079 AII66702.1    
 IY41_02640   Bacteroides dorei isolate HS2_L_2_B_045b ALA72499.1    
 BACINT_00347   Bacteroides intestinalis DSM 17393     5MUM[A]
 Bovatus_03783 / BACOVA_03493   Bacteroides ovatus ATCC 8483 ALJ48388.1   4IRT[A]
 BACOV975_01572   Bacteroides ovatus V975 SCV07798.1    
 Bacsa_1608   Bacteroides salanitronis DSM 18170 ADY36173.1    
 Btheta7330_04610   Bacteroides thetaiotaomicron 7330 ALJ44126.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase / exo-acting α-L-rhamnosidase (BT3686;BT_3686) 3.2.1.- Bacteroides thetaiotaomicron VPI-5482 AAO78791.1   5MUK[A]
5MUL[A]
 BvMPK_4254   Bacteroides vulgatus mpk ALK86796.1    
 BSFP_027170   Burkholderia stabilis FERMP-21014 BAX59873.1    
 BSFP_027150   Burkholderia stabilis FERMP-21014 BAX59871.1    
 SAMN06298216_2310   Cytophagales bacterium TFI 002 SOE21857.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (HMPREF9455_02360) 3.2.1.- Dysgonomonas gadei ATCC BAA-286 EGK01332.1    
 Echvi_0488   Echinicola vietnamensis DSM 17526 AGA76772.1    
 FLA_5507   Filimonas lacunae NBRC 104114 BAV09458.1    
 neuraminidase   Flavobacterium communis PK15 AOZ98835.1    
 GRFL_2251   Gramella flava JLT2011 APU68975.1    
 IMCC3135_10240   Granulosicoccus antarcticus IMCC3135 ASJ72142.1    
 YC6258_04555   Gynuella sunshinyii YC6258 AJQ96587.1    
 SAMN04515669_4177   Jiangella sp. DSM 45060 SDT46315.1    
 CEW81_00155 (fragment)   Kluyvera georgiana YDC799 ASG62411.1    
 Lbys_2519   Leadbetterella byssophila DSM 17132 ADQ18181.1    
 BBK82_39695   Lentzea guizhouensis DHS C013 ANZ43405.1    
 MROS_0979   Melioribacter roseus P3M-2 AFN74220.1    
 MILUP08_44594   Micromonospora lupini str. Lupac 08 CCH19719.1    
 Niako_0094   Niastella koreensis GR20-10 AEV96495.1    
 OPIT5_15820 (fragment)   Opitutaceae bacterium TAV5 AHF91469.1    
 OPIT5_06860   Opitutaceae bacterium TAV5 AHF89982.1    
 OPIT5_12240   Opitutaceae bacterium TAV5 AHF90862.1    
 Oter_1376   Opitutus terrae PB90-1 ACB74661.1 B1ZRY5  
 GAGA_0128   Paraglaciecola agarilytica NO2 GAC02993.1    
 GCHA_4714   Paraglaciecola chathamensis S18K6 GAC12631.1    
 Phep_0953   Pedobacter heparinus DSM 2366 ACU03175.1    
 neuraminidase   Pedobacter steynii DX4 AOM80927.1    
 STSP2_00746   Phycisphaerae bacterium ST-NAGAB-D1 AQT67598.1    
 neuraminidase   Polaribacter reichenbachii 6Alg 8T APZ45927.1    
 AW14_13375   Siansivirga zeaxanthinifaciens CC-SAMT-1 AJR04497.1    
 sce9313   Sorangium cellulosum So ce56 CAN99486.1    
 SCE1572_52500   Sorangium cellulosum So0157-2 AGP42384.1    
 CA265_06055   Sphingobacteriaceae bacterium GW460-11-11-14-LB5 ARS39259.1    
 Sph21_0793   Sphingobacterium sp. 21 ADZ77369.1    
 neuraminidase   Sphingobacterium sp. B29 APU96225.1    
 NX02_05120   Sphingomonas sanxanigenens DSM 19645 = NX02 AHE52764.1    
 BXU08_18035   Sphingomonas sp. LM7 AQR75307.1    
 BV401_37085   Streptomyces autolyticus CGMCC0516 AQA15193.1    
 SHXM_02217   Streptomyces hygroscopicus XM201 AQW48754.1    
 ABB07_03375   Streptomyces incarnatus NRRL 8089 AKJ09098.1    
 SLINC_7649   Streptomyces lincolnensis NRRL 2936 ANS69873.1    
 M271_09285   Streptomyces rapamycinicus NRRL 5491 AGP53470.1    
 CGL27_39655   Streptomyces sp. 11-1-2 ASQ98324.1    
 SAMN05444521_1021   Streptomyces sp. 3124.6 SHH58546.1    
 QR97_35310   Streptomyces sp. PBH53 AKN74302.1    
 A4E84_37460   Streptomyces sp. S10(2016) AMW14662.1    
 A4E84_36250   Streptomyces sp. S10(2016) AMW15654.1    
 CAG99_02725   Streptomyces sp. SCSIO 03032 ARQ67894.1    
 THTE_3910   Thermogutta terrifontis R1 ASV76511.1    
 neuraminidase   Verrucomicrobia bacterium HZ-65 ATC65312.1    
 IMCC26134_01360   Verrucomicrobia bacterium IMCC26134 AKC81760.1    
 ZPR_0909   Zunongwangia profunda SM-A87 ADF51256.1
YP_003583452.1
   
Top


Last update: 2017-10-09 © Copyright 1998-2017
AFMB - CNRS - Université d'Aix-Marseille