Glycoside Hydrolase Family 145

Activities in FamilyL-Rhα-α-1,4-GlcA α-L-rhamnohydrolase (EC 3.2.1.-)
Mechanism Retaining
3D Structure Statusseven-bladed β-propeller
Catalytic Nucleophile/BaseHis
NoteCreated after Munoz-Munoz et al., Proc. Natl. Acad. Sci. USA (2017) (PMID=28396425); these enzymes hydrolyze a-L-rhamnose residues linked to position 4 of a glucuronic acid using an unusual catalytic machinery comprising a histidine residue. The active site of these a-L-rhamnosidases is located on the opposite side of CAZymes with similar b-propeller folds. Surprisingly, the "normal" side of the b-propeller bears the highest residue conservation in the family and may well have another function. The latter is unknown but strong ressemblance to family PL25 suggests a polysaccharide lyase activity.
External resourcesCAZypedia;
Statistics GenBank accession (280); Uniprot accession (1); PDB accession (5); 3D entries (4); cryst (0)
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Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D
 TL08_16695   Actinoalloteichus hymeniacidonis HPA177(T) (=DSM 45092(T)) AOS64139.1    
 SAMN04489716_2891   Actinoplanes derwentensis DSM 43941 SDT21880.1    
 AFR_29060   Actinoplanes friuliensis DSM 7358 AGZ44073.1    
 Amir_4945   Actinosynnema mirum DSM 43827 ACU38771.1    
 APASM_5003   Actinosynnema pretiosum subsp. pretiosum ATCC 31280 AXX32368.1    
 CNX65_24385   Actinosynnema pretiosum X47 ATE56025.1    
 neuraminidase   Alkalitalea saponilacus DSM 24412 ASB47862.1    
 AMEC673_16885   Alteromonas macleodii str. English Channel 673 AFT76059.1    
 FJN15_15590   Alteromonas mediterranea AltCH17 QGX63106.1    
 FJN15_15290   Alteromonas mediterranea AltCH17 QGX63053.1    
 ALT761_04129   Alteromonas sp. 76-1 VEM00340.1    
 ALT761_04175   Alteromonas sp. 76-1 VEM00388.1    
 AMED_7434   Amycolatopsis mediterranei U32 ADJ49148.1    
 YIM_23735   Amycolatopsis sp. YIM 10 QFU89924.1    
 STSP2_00746   Anaerohalosphaera lusitana ST-NAGAB-D1 AQT67598.1    
 neuraminidase   Arachidicoccus sp. KIS59-12 AYD48252.1    
 Astex_3547   Asticcacaulis excentricus CB 48 ADU15177.1    
 EM6_3353   Asticcacaulis excentricus M6 BBF82712.1    
 CGC64_05340   Bacteroides caccae ATCC 43185 ASM67811.1    
 neuraminidase   Bacteroides caecimuris I48 ANU56237.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (BACCELL_00856) 3.2.1.- Bacteroides cellulosilyticus DSM 14838 EEF91490.1   5MVH[A]
 BcellWH2_01654   Bacteroides cellulosilyticus WH2 ALJ58907.1    
 ABI39_02695   Bacteroides dorei CL03T12C01 AND18477.1    
 neuraminidase   Bacteroides dorei DSM 17855 QJR78280.1    
 neuraminidase   Bacteroides dorei isolate HS1_L1_B_010 AII65648.1    
 GV66_02770   Bacteroides dorei isolate HS1_L3_B_079 AII66702.1    
 IY41_02640   Bacteroides dorei isolate HS2_L_2_B_045b ALA72499.1    
 neuraminidase   Bacteroides dorei JR01 QJR74128.1    
 neuraminidase   Bacteroides dorei JR02 QJR69795.1    
 neuraminidase   Bacteroides dorei JR03 QJR65533.1    
 neuraminidase   Bacteroides dorei JR04 QJR61378.1    
 neuraminidase   Bacteroides dorei JR05 QJR53822.1    
 neuraminidase   Bacteroides heparinolyticus F0111 AVM58295.1    
 neuraminidase   Bacteroides intestinalis APC919/174 QDO70678.1    
 neuraminidase   Bacteroides intestinalis APC919/174 QDO67478.1    
 BACINT_00347   Bacteroides intestinalis DSM 17393     5MUM[A]
 neuraminidase   Bacteroides ovatus 3725 D1 iv QDM11160.1    
 Bovatus_03783 / BACOVA_03493   Bacteroides ovatus ATCC 8483 ALJ48388.1   4IRT[A]
 neuraminidase   Bacteroides ovatus FDAARGOS_733 QGT73112.1    
 BACOV975_01572   Bacteroides ovatus V975 SCV07798.1    
 Bacsa_1608   Bacteroides salanitronis DSM 18170 ADY36173.1    
 neuraminidase   Bacteroides sp. CBA7301 QIU94068.1    
 Btheta7330_04610   Bacteroides thetaiotaomicron 7330 ALJ44126.1    
 BatF92_01830   Bacteroides thetaiotaomicron F9-2 BCA48241.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase / exo-acting α-L-rhamnosidase (BT3686;BT_3686) 3.2.1.- Bacteroides thetaiotaomicron VPI-5482 AAO78791.1   5MUK[A]
5MUL[A]
 Bun01g_00430   Bacteroides uniformis NBRC 113350 BBK85673.1    
 BvMPK_4254   Bacteroides vulgatus mpk ALK86796.1    
 VIC01_00644   Bacteroides vulgatus VIC01 QEW35176.1    
 neuraminidase   Bacteroides xylanisolvens H207 QDH54106.1    
 neuraminidase   Bacteroides zoogleoformans ATCC 33285 AVM53996.1    
 ACH79_15865 (fragment)   Bradyrhizobium sp. CCBAU 051011 QHO73899.1    
 DCN14_18050   Burkholderia sp. IDO3 AXK64598.1    
 WS87_04285   Burkholderia sp. MSMB0856 AOJ85942.1    
 WS87_04295   Burkholderia sp. MSMB0856 AOJ85944.1    
 BSFP_027170   Burkholderia stabilis FERMP-21014 BAX59873.1    
 BSFP_027150   Burkholderia stabilis FERMP-21014 BAX59871.1    
 C2869_06290   Catenovulum sp. CCB-QB4 AWB66072.1    
 E5225_06725   Cellulomonas shaoxiangyii Z28 QCB93288.1    
 Mal65_09100   Crateriforma conspicua Mal65 QDV61783.1    
 SAMN06298216_2310   Cytophagales bacterium TFI 002 SOE21857.1    
 neuraminidase   Duganella sp. AF9R3 QJD94036.1    
 neuraminidase   Duganella sp. GN2-R2 QJE01894.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (HMPREF9455_02360) 3.2.1.- Dysgonomonas gadei ATCC BAA-286 EGK01332.1    
 neuraminidase   Dysgonomonas sp. HDW5A QIK59154.1    
 neuraminidase   Dysgonomonas sp. HDW5B QIK53697.1    
 neuraminidase   Echinicola sp. LN3S3 QDH77890.1    
 DN752_08775   Echinicola strongylocentroti MEBiC08714 AWW30209.1    
 Echvi_0488   Echinicola vietnamensis DSM 17526 AGA76772.1    
 CWS02_23110 (fragment)   Enterobacter sp. EA-1 AUP77012.1    
 CWS02_23105 (fragment)   Enterobacter sp. EA-1 AUP77011.1    
 GN242_16660   Erwinia sp. J780 QGU88756.1    
 FLA_5507   Filimonas lacunae NBRC 104114 BAV09458.1    
 EJO69_05305   Flaviflexus salsibiostraticola KCTC 33148 AZN29787.1    
 neuraminidase   Flavihumibacter sp. SB-02 QGW27552.1    
 neuraminidase   Flavisolibacter ginsenosidimutans Gsoil 636 QEC58411.1    
 neuraminidase   Flavobacterium commune PK15 AOZ98835.1    
 HYN56_19350   Flavobacterium crocinum HYN0056 AWK06268.1    
 neuraminidase   Flavobacterium sp. KBS0721 QDW22445.1    
 neuraminidase   Flavobacterium sp. SLB02 SGTM QGK74231.1    
 neuraminidase   Georgenia sp. Z294 QDB80652.1    
 GRFL_2251   Gramella flava JLT2011 APU68975.1    
 neuraminidase   Gramella fulva SH35 AVR47414.1    
 IMCC3135_10240   Granulosicoccus antarcticus IMCC3135 ASJ72142.1    
 YC6258_04555   Gynuella sunshinyii YC6258 AJQ96587.1    
 neuraminidase   Hymenobacter jejuensis 17J68-5 QDA58744.1    
 SAMN04515669_4177   Jiangella sp. DSM 45060 SDT46315.1    
 GT409_02010   Kiritimatiellaeota bacterium S-5007 QHI68278.1    
 NCTC9668_00292   Klebsiella aerogenes NCTC9668 VEC95070.1    
 DA718_12825   Klebsiella huaxiensis WCHKl090001 QBG08014.1    
 CEW81_00155 (fragment)   Kluyvera georgiana YDC799 ASG62411.1    
 GHC38_00095   Kluyvera intermedia HR2 QGH37132.1    
 GHC21_00095   Kluyvera intermedia N2-1 QGH28150.1    
 NCTC12125_03813 (fragment)   Kluyvera intermedia NCTC12125 VDZ85608.1    
 GY169_00395   Kluyvera sp. PO2S7 QIR25340.1    
 Lbys_2519   Leadbetterella byssophila DSM 17132 ADQ18181.1    
 BBK82_39695   Lentzea guizhouensis DHS C013 ANZ43405.1    
 HHL09_22945   Luteolibacter sp. G-1-1-1 QJE98521.1    
 FE772_18170   Lysobacter enzymogenes YC36 QCW27274.1    
 MFFC18_27830 (fragment)   Mariniblastus fucicola FC18 QEG22895.1    
 MFFC18_27850   Mariniblastus fucicola FC18 QEG22897.1    
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