Glycoside Hydrolase Family 145

Activities in FamilyL-Rhα-α-1,4-GlcA α-L-rhamnohydrolase (EC 3.2.1.-)
Mechanism Retaining
3D Structure Statusseven-bladed β-propeller
Catalytic Nucleophile/BaseHis
NoteCreated after Munoz-Munoz et al., Proc. Natl. Acad. Sci. USA (2017) (PMID=28396425); these enzymes hydrolyze a-L-rhamnose residues linked to position 4 of a glucuronic acid using an unusual catalytic machinery comprising a histidine residue. The active site of these a-L-rhamnosidases is located on the opposite side of CAZymes with similar b-propeller folds. Surprisingly, the "normal" side of the b-propeller bears the highest residue conservation in the family and may well have another function. The latter is unknown but strong ressemblance to family PL25 suggests a polysaccharide lyase activity.
External resourcesCAZypedia;
Statistics GenBank accession (225); Uniprot accession (1); PDB accession (5); 3D entries (4); cryst (0)
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D
 TL08_16695   Actinoalloteichus hymeniacidonis HPA177(T) (=DSM 45092(T)) AOS64139.1    
 SAMN04489716_2891   Actinoplanes derwentensis DSM 43941 SDT21880.1    
 AFR_29060   Actinoplanes friuliensis DSM 7358 AGZ44073.1    
 Amir_4945   Actinosynnema mirum DSM 43827 ACU38771.1    
 APASM_5003   Actinosynnema pretiosum subsp. pretiosum ATCC 31280 AXX32368.1    
 CNX65_24385   Actinosynnema pretiosum X47 ATE56025.1    
 neuraminidase   Alkalitalea saponilacus DSM 24412 ASB47862.1    
 AMEC673_16885   Alteromonas macleodii str. English Channel 673 AFT76059.1    
 ALT761_04129   Alteromonas sp. 76-1 VEM00340.1    
 ALT761_04175   Alteromonas sp. 76-1 VEM00388.1    
 AMED_7434   Amycolatopsis mediterranei U32 ADJ49148.1    
 YIM_23735   Amycolatopsis sp. YIM 10 QFU89924.1    
 neuraminidase   Arachidicoccus sp. KIS59-12 AYD48252.1    
 Astex_3547   Asticcacaulis excentricus CB 48 ADU15177.1    
 EM6_3353   Asticcacaulis excentricus M6 BBF82712.1    
 CGC64_05340   Bacteroides caccae ATCC 43185 ASM67811.1    
 neuraminidase   Bacteroides caecimuris I48 ANU56237.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (BACCELL_00856) 3.2.1.- Bacteroides cellulosilyticus DSM 14838 EEF91490.1   5MVH[A]
 BcellWH2_01654   Bacteroides cellulosilyticus WH2 ALJ58907.1    
 ABI39_02695   Bacteroides dorei CL03T12C01 AND18477.1    
 neuraminidase   Bacteroides dorei isolate HS1_L1_B_010 AII65648.1    
 GV66_02770   Bacteroides dorei isolate HS1_L3_B_079 AII66702.1    
 IY41_02640   Bacteroides dorei isolate HS2_L_2_B_045b ALA72499.1    
 neuraminidase   Bacteroides heparinolyticus F0111 AVM58295.1    
 neuraminidase   Bacteroides intestinalis APC919/174 QDO70678.1    
 neuraminidase   Bacteroides intestinalis APC919/174 QDO67478.1    
 BACINT_00347   Bacteroides intestinalis DSM 17393     5MUM[A]
 neuraminidase   Bacteroides ovatus 3725 D1 iv QDM11160.1    
 Bovatus_03783 / BACOVA_03493   Bacteroides ovatus ATCC 8483 ALJ48388.1   4IRT[A]
 BACOV975_01572   Bacteroides ovatus V975 SCV07798.1    
 Bacsa_1608   Bacteroides salanitronis DSM 18170 ADY36173.1    
 Btheta7330_04610   Bacteroides thetaiotaomicron 7330 ALJ44126.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase / exo-acting α-L-rhamnosidase (BT3686;BT_3686) 3.2.1.- Bacteroides thetaiotaomicron VPI-5482 AAO78791.1   5MUK[A]
5MUL[A]
 Bun01g_00430   Bacteroides uniformis NBRC 113350 BBK85673.1    
 BvMPK_4254   Bacteroides vulgatus mpk ALK86796.1    
 VIC01_00644   Bacteroides vulgatus VIC01 QEW35176.1    
 neuraminidase   Bacteroides xylanisolvens H207 QDH54106.1    
 neuraminidase   Bacteroides zoogleoformans ATCC 33285 AVM53996.1    
 DCN14_18050   Burkholderia sp. IDO3 AXK64598.1    
 WS87_04285   Burkholderia sp. MSMB0856 AOJ85942.1    
 WS87_04295   Burkholderia sp. MSMB0856 AOJ85944.1    
 BSFP_027170   Burkholderia stabilis FERMP-21014 BAX59873.1    
 BSFP_027150   Burkholderia stabilis FERMP-21014 BAX59871.1    
 C2869_06290   Catenovulum sp. CCB-QB4 AWB66072.1    
 E5225_06725   Cellulomonas sp. Z28 QCB93288.1    
 SAMN06298216_2310   Cytophagales bacterium TFI 002 SOE21857.1    
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (HMPREF9455_02360) 3.2.1.- Dysgonomonas gadei ATCC BAA-286 EGK01332.1    
 neuraminidase   Echinicola sp. LN3S3 QDH77890.1    
 DN752_08775   Echinicola strongylocentroti MEBiC08714 AWW30209.1    
 Echvi_0488   Echinicola vietnamensis DSM 17526 AGA76772.1    
 CWS02_23110 (fragment)   Enterobacter sp. EA-1 AUP77012.1    
 CWS02_23105 (fragment)   Enterobacter sp. EA-1 AUP77011.1    
 FLA_5507   Filimonas lacunae NBRC 104114 BAV09458.1    
 EJO69_05305   Flaviflexus salsibiostraticola KCTC 33148 AZN29787.1    
 neuraminidase   Flavisolibacter ginsenosidimutans Gsoil 636 QEC58411.1    
 neuraminidase   Flavobacterium commune PK15 AOZ98835.1    
 HYN56_19350   Flavobacterium crocinum HYN0056 AWK06268.1    
 neuraminidase   Flavobacterium sp. KBS0721 QDW22445.1    
 neuraminidase   Georgenia sp. Z294 QDB80652.1    
 GRFL_2251   Gramella flava JLT2011 APU68975.1    
 neuraminidase   Gramella sp. SH35 AVR47414.1    
 IMCC3135_10240   Granulosicoccus antarcticus IMCC3135 ASJ72142.1    
 YC6258_04555   Gynuella sunshinyii YC6258 AJQ96587.1    
 neuraminidase   Hymenobacter sp. 17J68-5 QDA58744.1    
 SAMN04515669_4177   Jiangella sp. DSM 45060 SDT46315.1    
 NCTC9668_00292   Klebsiella aerogenes NCTC9668 VEC95070.1    
 DA718_12825   Klebsiella sp. WCHKl090001 QBG08014.1    
 CEW81_00155 (fragment)   Kluyvera georgiana YDC799 ASG62411.1    
 NCTC12125_03813 (fragment)   Kluyvera intermedia NCTC12125 VDZ85608.1    
 Lbys_2519   Leadbetterella byssophila DSM 17132 ADQ18181.1    
 BBK82_39695   Lentzea guizhouensis DHS C013 ANZ43405.1    
 FE772_18170   Lysobacter enzymogenes YC36 QCW27274.1    
 MFFC18_27830 (fragment)   Mariniblastus fucicola FC18 QEG22895.1    
 MFFC18_27850   Mariniblastus fucicola FC18 QEG22897.1    
 CJ739_3933   Mariniflexile sp. TRM1-10 AXP82991.1    
 EYF70_08645   Massilia albidiflava DSM 17472 QBI00910.1    
 neuraminidase   Massilia lutea DSM 17473 QBE67449.1    
 DIR46_11140 (neuraminidase)   Massilia timonae CCUG 43427 AWL04923.1    
 neuraminidase   Massilia umbonata DSMZ 26121 QCP10739.1    
 MROS_0979   Melioribacter roseus P3M-2 AFN74220.1    
 CQW49_07015   Methylosinus trichosporium OB3b ATQ67665.1    
 CQW49_23070   Methylosinus trichosporium OB3b ATQ70845.1    
 DXT68_02925   Microbacterium foliorum NRRL B-24224 AXL11201.1    
 C6C15_15090   Microbacterium sp. str. China AVL98316.1    
 CSX12_16590   Microbacterium sp. Y-01 AZH79949.1    
 MILUP08_44594   Micromonospora lupini str. Lupac 08 CCH19719.1    
 neuraminidase   Mucilaginibacter ginsenosidivorax KHI28 QEC76455.1    
 neuraminidase   Mucilaginibacter gossypii P4 QEM15375.1    
 neuraminidase   Mucilaginibacter rubeus P1 QEM11342.1    
 neuraminidase   Mucilaginibacter rubeus P2 QEM02756.1    
 neuraminidase   Muribaculum sp. TLL-A4 QCD37093.1    
 Niako_0094   Niastella koreensis GR20-10 AEV96495.1    
 EXE57_02480   Nocardioides sp. MMS17-SY117 QBR91261.1    
 neuraminidase   Olivibacter sp. LS-1 QEK99377.1    
 OPIT5_15820 (fragment)   Opitutaceae bacterium TAV5 AHF91469.1    
 OPIT5_06860   Opitutaceae bacterium TAV5 AHF89982.1    
 OPIT5_12240   Opitutaceae bacterium TAV5 AHF90862.1    
 OPIT5_28600   Opitutaceae bacterium TAV5 AHF93563.1    
 Oter_1376   Opitutus terrae PB90-1 ACB74661.1 B1ZRY5  
 E6C60_3333   Paenibacillus sp. HB172198 QCT04044.1    
 GAGA_0128   Paraglaciecola agarilytica NO2 GAC02993.1    
 GCHA_4714   Paraglaciecola chathamensis S18K6 GAC12631.1    
 Phep_0953   Pedobacter heparinus DSM 2366 ACU03175.1    
 neuraminidase   Pedobacter sp. CJ43 QEK51395.1    
 neuraminidase   Pedobacter sp. KBS0701 QDW25600.1    
 neuraminidase   Pedobacter steynii DX4 AOM80927.1    
 STSP2_00746   Phycisphaerae bacterium ST-NAGAB-D1 AQT67598.1    
 PLANPX_0435   Planctomycetales bacterium PX69 BBO30823.1    
 Enr13x_39200   Planctomycetes bacterium Enr13 QDV44059.1    
 K2D_06720   Planctomycetes bacterium K2D QDV77085.1    
 Mal65_09100   Planctomycetes bacterium Mal65 QDV61783.1    
 MalM25_16610   Planctomycetes bacterium MalM25 QDT68736.1    
 MalM25_16520   Planctomycetes bacterium MalM25 QDT68727.1    
 Pan181_31490   Planctomycetes bacterium Pan181 QDU56937.1    
 Pan181_12980   Planctomycetes bacterium Pan181 QDU55112.1    
 Pan265_13080   Planctomycetes bacterium Pan265 QDU71458.1    
 Pla163_03170   Planctomycetes bacterium Pla163 QDU83219.1    
 Spa11_06910   Planctomycetes bacterium Spa11 QDV72513.1    
 C6W10_03295   Plantactinospora sp. BB1 AVT35643.1    
 C6361_06725   Plantactinospora sp. BC1 AVT29231.1    
 EAO79_03990   Plantibacter sp. PA-3-X8 AZH82150.1    
 neuraminidase   Polaribacter reichenbachii 6Alg 8T APZ45927.1    
 neuraminidase   Polaribacter reichenbachii KCTC 23969 AUC19789.1    
 neuraminidase   Pseudoflavitalea sp. 5GH32-13 AXY77893.1    
 AL527_05800   Pseudomonas fulva FDAARGOS_167 AVF54731.1    
 NCTC13098_02598 (fragment)   Raoultella terrigena NCTC13098 VDR26257.1    
 C7V51_06805   Rathayibacter iranicus NCCPB 2253 AZZ55625.1    
 AW14_13375   Siansivirga zeaxanthinifaciens CC-SAMT-1 AJR04497.1    
 sce9313   Sorangium cellulosum So ce56 CAN99486.1    
 SOCE836_108110   Sorangium cellulosum So ce836 AUX38564.1    
 SOCEGT47_037240   Sorangium cellulosum So ceGT47 AUX23201.1    
 SCE1572_52500   Sorangium cellulosum So0157-2 AGP42384.1    
 CA265_06055   Sphingobacteriaceae bacterium GW460-11-11-14-LB5 ARS39259.1    
 neuraminidase   Sphingobacterium psychroaquaticum SJ-25 QBQ40808.1    
 Sph21_0793   Sphingobacterium sp. 21 ADZ77369.1    
 neuraminidase   Sphingobacterium sp. B29 APU96225.1    
 NCTC11429_01309   Sphingobacterium thalpophilum NCTC11429 VTR34304.1    
 E5554_14945   Sphingobium sp. PAMC28499 QCB39004.1    
 A6768_08740   Sphingobium yanoikuyae S72 ATI80083.1    
 BV87_15370   Sphingobium yanoikuyae SHJ ATP21553.1    
 EBF16_10890   Sphingobium yanoikuyae SJTF8 AYO77339.1    
 NX02_05120   Sphingomonas sanxanigenens DSM 19645 = NX02 AHE52764.1    
 CVN68_10755   Sphingomonas sp. Cra20 ATY32394.1    
 BXU08_18035   Sphingomonas sp. LM7 AQR75307.1    
 EKD16_15315   Streptomonospora sp. M2 QBI54840.1    
 DMT42_34610   Streptomyces actuosus ATCC 25421 AWT46891.1    
 CD934_31805   Streptomyces asterosporus DSM 41452 QDI72772.1    
 C5746_39180   Streptomyces atratus SCSIO_ZH16 AXE81955.1    
 BV401_37085   Streptomyces autolyticus CGMCC0516 AQA15193.1    
 SAVMC3_84080   Streptomyces avermitilis MC3 BBJ55779.1    
 EJC51_41055   Streptomyces brunneus CR22 AZP21923.1    
 CP983_03380   Streptomyces chartreusis ATCC 14922 QEV65825.1    
 CP976_38850   Streptomyces coeruleorubidus ATCC 13740 QEV29484.1    
 CP966_32485   Streptomyces galilaeus ATCC 14969 QEU69464.1    
 DWG14_01012   Streptomyces griseorubiginosus 3E-1 AYC36802.1    
 ELQ87_03215   Streptomyces griseoviridis F1-27 AZS83413.1    
 ELQ87_38825   Streptomyces griseoviridis F1-27 AZS89544.1    
 CEB94_37610   Streptomyces hawaiiensis ATCC 12236 QCD59870.1    
 SHXM_02217   Streptomyces hygroscopicus XM201 AQW48754.1    
 ABB07_03375   Streptomyces incarnatus NRRL 8089 AKJ09098.1    
 SLCG_7636   Streptomyces lincolnensis LC-G AXG58791.1    
 SLINC_7649   Streptomyces lincolnensis NRRL 2936 ANS69873.1    
 SMALA_6697 (fragment)   Streptomyces malaysiensis DSM 4137 ATL86917.1
QDL69581.1
   
 CP967_01715   Streptomyces nitrosporeus ATCC 12769 QEU70842.1    
 CP972_32645   Streptomyces prasinus ATCC 13879 QEV09719.1    
 A4E84_37460   Streptomyces qaidamensis S10(2016) AMW14662.1    
 A4E84_36250   Streptomyces qaidamensis S10(2016) AMW15654.1    
 M271_09285   Streptomyces rapamycinicus NRRL 5491 AGP53470.1    
 CGL27_39655   Streptomyces sp. 11-1-2 ASQ98324.1    
 SAMN05444521_1021   Streptomyces sp. 3214.6 SHH58546.1    
 CXR04_32235   Streptomyces sp. CMB-StM0423 AUH44245.1    
 C1703_36835   Streptomyces sp. Go-475 AXE90615.1    
 DVK44_34825   Streptomyces sp. GSSD-12 AXG82054.1    
 F9278_43055   Streptomyces sp. GY16 QFR01841.1    
 CFP59_01977   Streptomyces sp. M56 AUA09887.1    
 EJ357_03640   Streptomyces sp. MK-45 AZQ32648.1    
 C6376_25320   Streptomyces sp. P3 AVV44267.1    
 QR97_35310   Streptomyces sp. PBH53 AKN74302.1    
 FNV59_47370   Streptomyces sp. RLB1-8 QDO64728.1    
 FNV58_48315   Streptomyces sp. RLB1-9 QDO02725.1    
 FNV62_46715   Streptomyces sp. RLB3-17 QDO44595.1    
 FNV60_45115   Streptomyces sp. RLB3-5 QDO54483.1    
 FNV61_47905   Streptomyces sp. RLB3-6 QDN92214.1    
 FNV63_46920   Streptomyces sp. S1A1-3 QDO34581.1    
 FNV64_48335   Streptomyces sp. S1A1-7 QDN82328.1    
 FNV65_46900   Streptomyces sp. S1A1-8 QDO24460.1    
 FNV66_46635   Streptomyces sp. S1D4-14 QDN72025.1    
 FNV67_47785   Streptomyces sp. S1D4-20 QDN61973.1    
 FNV68_48975   Streptomyces sp. S1D4-23 QDO13039.1    
 D7Y56_26145   Streptomyces sp. S501 QBR09101.1    
 CAG99_02725   Streptomyces sp. SCSIO 03032 ARQ67894.1    
 DLM49_02270   Streptomyces sp. WAC 01438 AZM58535.1    
 DMB38_02170   Streptomyces sp. WAC 06738 AZM44779.1    
 DMB38_30870   Streptomyces sp. WAC 06738 AZM49594.1    
 SSPO_021070   Streptomyces sporoclivatus NBRC 100767 BBJ39389.1    
 THTE_3910   Thermogutta terrifontis R1 ASV76511.1    
 neuraminidase   Verrucomicrobia bacterium HZ-65 ATC65312.1    
 IMCC26134_01360   Verrucomicrobia bacterium IMCC26134 AKC81760.1    
 ZPR_0909   Zunongwangia profunda SM-A87 ADF51256.1
YP_003583452.1
   
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3D
 BCIN_01g05080   Botrytis cinerea B05.10 ATZ45789.1    
 BofuT4_P044640.1   Botrytis cinerea T4 CCD45446.1    
 SAMEA4029010_CIC11G00000001359   [Candida] intermedia CBS 141442 SGZ47226.1    
 SAMEA4029009_CIC11G00000000585   [Candida] intermedia PYCC 4715 SGZ49612.1    
 SETTUDRAFT_33355   Exserohilum turcica Et28A EOA84368.1    
 FFUJ_14848   Fusarium fujikuroi IMI 58289 CCT67810.1    
 FGRAMPH1_01T21617   Fusarium graminearum PH-1 SCB65023.1    
 FVRRES_10681   Fusarium venenatum A3/5 CEI70604.1    
 FVRRES_08057   Fusarium venenatum A3/5 CEI67980.1    
 UCRNP2_6875 (fragment)   Neofusicoccum parvum UCRNP2 EOD46375.1    
 NCU00990   Neurospora crassa OR74A EAA34664.3    
 Putative protein of unknown function (fragment)   Podospora anserina S mat+ CDP22308.1
CAP59667.1
   
 PODCO_100820   Podospora comata VBB71297.1    
 PoMZ_06168   Pyricularia oryzae QBZ64470.1    
 MGG_14899   Pyricularia oryzae 70-15 EHA47224.1    
 SAPIO_CDS0694   Scedosporium apiospermum IHEM 14462 KEZ46379.1    
 SCHCODRAFT_232948   Schizophyllum commune H4-8 EFJ01384.1    
 SERLA73DRAFT_161488   Serpula lacrymans var. lacrymans S7.3 EGN97454.1    
 M422DRAFT_185901   Sphaerobolus stellatus SS14 KIJ31449.1    
 MYCTH_2307864   Thermothelomyces thermophilus ATCC 42464 AEO59504.1    
 THITE_2120556   Thermothielavioides terrestris NRRL 8126 AEO69808.1    
 BN946_scf185016.g41   Trametes cinnabarina BRFM137 CDO73884.1    
 TRAVEDRAFT_174832   Trametes versicolor FP-101664 SS1 EIW52757.1    
 CANTEDRAFT_94676   Yamadazyma tenuis ATCC 10573 EGV61793.1    
Top


Last update: 2019-11-09 © Copyright 1998-2019
AFMB - CNRS - Université d'Aix-Marseille