Glycoside Hydrolase Family 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (60437); Uniprot accession (7858); PDB accession (485); 3D entries (113); cryst (2)
All (55616) Archaea (381) Bacteria (51891) Eukaryota (3214) unclassified (130) Structure (113 - 2 cryst) Characterized (813)
| 1 | ... | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 |
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 amylase-2   Tetraodon nigroviridis CAC87126.1 Q8UWE4   24
 amylase-3 3.2.1.1 Tetraodon nigroviridis CAC87127.1 Q8UWE3   24
 amylase   Tetraodon nigroviridis CAD20312.1
CAD20263.1
Q8QFS2   24
 TBLA_0C01380 (Tbla0c01380)   Tetrapisispora blattae CBS 6284 type strain:CBS 6284 CCH59953.1     8
 TBLA_0J00110 (Tbla0j00110)   Tetrapisispora blattae CBS 6284 type strain:CBS 6284 CCH63012.1     25
 TPHA_0B03910 (Tpha0b03910)   Tetrapisispora phaffii CBS 4417 CCE62063.1     8
 TPHA_0I03290 (Tpha0i03290)   Tetrapisispora phaffii CBS 4417 CCE64830.1     25
 α-amylase (Amy) (fragment)   Thaumatomyia notata AAY88858.1 Q3SC90   15
 α-amylase (Amy) (fragment)   Thaumatomyia notata AAY88859.1 Q3SC89   15
 α-amylase 3.2.1.1 Thermomyces lanuginosus CAA03110.1
CAA03111.1
    1
 oligo-1,6-glucosidase (OgL)   Thermomyces lanuginosus SSBP APY25376.1     40
 MYCTH_2295601   Thermothelomyces thermophila ATCC 42464 AEO53795.1     25
 MYCTH_2303065   Thermothelomyces thermophila ATCC 42464 AEO57204.1     40
 MYCTH_2303089   Thermothelomyces thermophila ATCC 42464 AEO57214.1     40
 MYCTH_108416   Thermothelomyces thermophila ATCC 42464 AEO58445.1     22
 MYCTH_2305783   Thermothelomyces thermophila ATCC 42464 AEO58446.1     1
 MYCTH_69975   Thermothelomyces thermophila ATCC 42464 AEO58507.1     5
 MYCTH_2312374   Thermothelomyces thermophila ATCC 42464 AEO61801.1     8
 MYCTH_2122625 (fragment)   Thermothelomyces thermophila ATCC 42464 AEO53757.1    
 MYCTH_77082   Thermothelomyces thermophila ATCC 42464 AEO54195.1     1
 THITE_2114181   Thielavia terrestris NRRL 8126 AEO66220.1     25
 THITE_2146540   Thielavia terrestris NRRL 8126 AEO69575.1     40
 THITE_2056265   Thielavia terrestris NRRL 8126 AEO70654.1     40
 THITE_2124116   Thielavia terrestris NRRL 8126 AEO71574.1     8
 THITE_152693   Thielavia terrestris NRRL 8126 AEO65810.1     1
 secreted protein   Thraustotheca clavata ATCC 34112 AIG55646.1    
 secreted protein   Thraustotheca clavata ATCC 34112 AIG55646.1     1
 secreted protein   Thraustotheca clavata ATCC 34112 AIG55673.1     1
 amylase-2   Thunnus orientalis BAL14132.1     24
 amylase-1   Thunnus orientalis BAL14131.1     24
 α-amylase (Amy2) (fragment)   Thunnus thynnus CAX33863.1 B9WPP5   24
 TDEL_0B05680 (Tdel0b05680)   Torulaspora delbrueckii type strain:CBS 1146 CCE90697.1     8
 TDEL_0B07670 (Tdel0b07670)   Torulaspora delbrueckii type strain:CBS 1146 CCE90896.1     40
 TDEL_0D06530 (Tdel0d06530)   Torulaspora delbrueckii type strain:CBS 1146 CCE92237.1
AAQ75122.1
Q5J9B3   40
 TDEL_0H00300 (Tdel0h00300)   Torulaspora delbrueckii type strain:CBS 1146 CCE93889.1     25
 TDEL_0A00110 (Tdel0a00110)   Torulaspora delbrueckii type strain:CBS 1146 CCE89343.1     40
 ORF   Toxoplasma gondii AAU01394.1 Q5IXJ0  
 ORF   Toxoplasma gondii AAU01393.1 Q5IXJ1   9
 TgIb.2080   Toxoplasma gondii RH CAJ20682.1 Q1JSE3   8
 TgIb.2080   Toxoplasma gondii RH CAJ20682.1 Q1JSE3   8
 α-amylase   Tribolium castaneum CAA30009.1
AFO37738.1
P09107   15
 α-amylase I 3.2.1.1 Tribolium castaneum AAA03708.1
AGW27504.1
AGW27506.1
Q26854   15
 α-amylase II 3.2.1.1 Tribolium castaneum AAA03709.1 Q26855   15
 α-amylase   Tribolium castaneum AGW27505.1     15
 α-amylase (TRa2) 3.2.1.1 Trichoderma viride IAM 5141 / JCM22452 BAG69580.1 B5BQC3   1
 glycoside hydrolase family 13 protein (Tmamy1)   Tricholoma matsutake ATCC 64715 BBA80377.1     32
 glycoside hydrolase family 13 protein (Tmamy1)   Tricholoma matsutake J-1 BBC62173.1     32
 glycoside hydrolase family 13 protein (Tmamy1)   Tricholoma matsutake NBRC 30604 BBA80373.1     32
 glycoside hydrolase family 13 protein (Tmamy1)   Tricholoma matsutake NBRC 30605 BBA80374.1     32
 glycoside hydrolase family 13 protein (Tmamy1)   Tricholoma matsutake NBRC 30773 BBA80375.1     32
 glycoside hydrolase family 13 protein (Tmamy1)   Tricholoma matsutake NBRC 33137 BBA80376.1     32
 glycoside hydrolase family 13 protein (Tmamy1)   Tricholoma matsutake NBRC 6933 BBA80372.1     32
 glycoside hydrolase family 13 protein (Tmamy1)   Tricholoma matsutake Z-1 BBC62174.1     32
 TVAG_185930   Trichomonas vaginalis G3 ATCC PRA-98 EAY23285.1 A2D8N6  
 sucrose hydrolase (TvSuh)   Trilocha varians BAP18684.1     17
 amylase, partial (fragment)   Triticum aestivum AMC30631.1     6
 α-amylase 2, partial (fragment)   Triticum aestivum ANZ54399.1     6
 α-amylase 3   Triticum aestivum ANZ54396.1     6
 α-amylase 2, partial (fragment)   Triticum aestivum ANZ54397.1     6
 α-amylase 3   Triticum aestivum ANZ54395.1     6
 α-amylase 2, partial (fragment)   Triticum aestivum ANZ54398.1     6
 α-amylase 1   Triticum aestivum ATY36095.1     6
 α-amylase 4   Triticum aestivum ATY36105.1     6
 α-amylase 1   Triticum aestivum ATY36099.1     6
 α-amylase 4   Triticum aestivum ATY36106.1     6
 α-amylase 1   Triticum aestivum ATY36094.1     6
 α-amylase 2   Triticum aestivum ATY36102.1     6
 α-amylase 1   Triticum aestivum ATY36098.1     6
 α-amylase 4   Triticum aestivum ATY36107.1     6
 α-amylase 2   Triticum aestivum ATY36103.1     6
 α-amylase 2   Triticum aestivum ATY36101.1     6
 α-amylase 1   Triticum aestivum ATY36093.1     6
 α-amylase 2   Triticum aestivum ATY36104.1     6
 α-amylase 1   Triticum aestivum ATY36097.1     6
 α-amylase 1   Triticum aestivum ATY36100.1     6
 α-amylase 1   Triticum aestivum ATY36096.1     6
 isoamylase wDBE-D1   Triticum aestivum AAP44580.1 Q7XA15   11
 Sequence 54 from patent US 6730825   Triticum aestivum AAU99042.1
ACM95339.1
    8
 isoamylase 3 (Isa3;TaISA3) 3.2.1.68 Triticum aestivum AEV92948.1     11
 starch branching enzyme III   Triticum aestivum AFH58741.1     8
 starch branching enzyme IIb (SBE IIb)   Triticum aestivum AAW80631.1 Q24M29   8
 isoamylase II, partial (fragment)   Triticum aestivum AFU92143.1     11
 α-amylase 3.2.1.1 Triticum aestivum AAA34259.1
CAA29252.1
CAZ78894.1
P08117   6
 branching enzyme 2.4.1.18 Triticum aestivum AAB17086.1
AAQ37450.1
O24397   8
 branching enzyme I 2.4.1.18 Triticum aestivum CAB40980.1
AAG27622.1
CAB40979.1
CAB40981.1
Q9FUU8
Q9XGB2
  8
 branching enzyme I 2.4.1.18 Triticum aestivum AAU99038.1
ABN48660.1
ACM95335.1
CAA72987.1
O04074   8
 branching enzyme I   Triticum aestivum AAB61925.1
AAU99030.1
AAU99037.1
ACM95327.1
ACM95334.1
O24393   8
 branching enzyme II 2.4.1.18 Triticum aestivum CAA72154.1
AAU99020.1
AAU99039.1
ABU93825.1
ABU93826.1
ABU93827.1
ABU93828.1
ACF32780.1
ACF32781.1
ACF32785.1
ACM95317.1
ACM95336.1
CCD41775.1
P93691   8
 isoamylase 1 (fragment)   Triticum aestivum AAD33890.1 Q9XFG7   11
 putative amylase (fragment)   Triticum aestivum CAA31917.1 P11786  
 Sequence 2 from patent US 7041484   Triticum aestivum ABH73745.1     8
 Sequence 2 from patent US 7041484   Triticum aestivum ABH73745.1     8
 Sequence 4 from patent US 7074579   Triticum aestivum ABI01021.1
AHB20173.1
AHY18982.1
AHY18983.1
    6
 high pI α-amylase (Amy1-1A) (fragment)   Triticum aestivum AHB20171.1     6
 high pI α-amylase (Amy1-1B) (fragment)   Triticum aestivum AHB20172.1     6
 high pI α-amylase (Amy1-2B) (fragment)   Triticum aestivum AHB20174.1     6
 high pI α-amylase (Amy1-3A) (fragment)   Triticum aestivum AHB20175.1     6
 Sequence 106729 from patent US 7214786   Triticum aestivum ABT19259.1     11
 isoamylase 3.2.1.68 Triticum aestivum AAL31015.1
AAL31016.1
AAY75324.1
ABA70415.1
ABA70416.1
CAC07514.1
CAC07515.1
CAC41016.2
CAC82925.1
Q8VWM3
Q8VWN0
Q8W546
Q8W547
  11
 starch branching enzyme IIa (fragment)   Triticum aestivum ACF32789.1 B4YSG1   8
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