Activities in Sub Family	[retaining] α-1,4-glucan branching enzyme (EC 2.4.1.18); [retaining] α-amylase (EC 3.2.1.1);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (1076); Uniprot accession (59); PDB accession (10); 3D entries (3); cryst (0)

Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
branching-enzyme (Gbe1;HosBE)	2.4.1.18	Homo sapiens	AAA58642.1 AAH12098.1 ABE12601.1 BAD92968.1 BAG54265.1 BAG60255.1 BAG62313.1 NP_000149.1	B4DNJ3 B4DUF1 Q04446 Q04446 Q59ET0	4BZY[A,B,C] 2.75 5CLT[A,B,C] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.79 5CLW[A,B,C] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.80	8
α-amylase (CAGL0M03377g)	3.2.1.1	Nakaseomyces glabratus CBS 138	CAG62470.1	Q6FJV0	7P43[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.93 7P44[A,B] 2.40 7P45[A] 2.09	8
starch-branching enzyme 1 (Sbe1;Rbe1;BEI;OsBEI;Os06g0726400)	2.4.1.18	Oryza sativa Japonica Group	BAF20543.1 AAD28284.1 AAP68993.1 AAU99032.1 AAX83629.1 ABA16988.1 ABD15942.1 ABD15943.1 ABD15944.1 ABD15945.1 ABE12599.1 ABL74557.1 ABL74558.1 ACM95329.1 ACY56114.1 ADK61026.1 ADK61027.1 AEB33747.1 AEB33748.1 AEB33749.1 AEB33750.1 AEB33751.1 AEB33752.1 AEB33753.1 AEB33754.1 AHL84201.1 AHL84202.1 AHL84212.1 AII21924.1 BAA01584.1 BAA01616.1 BAA01855.1 BAD61712.1 BAD61713.1 BAD61804.1 BAD61805.1 BAG89369.1 BAS99598.1 BAS99599.1 BAS99600.1	A0A076FSW4 A0A0P0X161 A0A0P0X168 A1YQH7 A1YQH8 B7EAH2 Q01401 Q0D9D0 Q7XZP9	3AMK[A] 1.90 3AML[A] 1.70 3VU2[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp β-D-Glcp 2.23 7ML5[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.35	8
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