Glycoside Hydrolase Family 13 / Subf 8

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (1355); Uniprot accession (188); PDB accession (6); 3D entries (2); cryst (0)
Eukaryota
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 branching-enzyme (Gbe1;HosBE) 2.4.1.18 Homo sapiens AAA58642.1
AAH12098.1
ABE12601.1
BAD92968.1
BAG54265.1
BAG60255.1
BAG62313.1
NP_000149.1
Q04446
Q04446
4BZY[A,B,C] 2.75
5CLT[A,B,C] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.79
5CLW[A,B,C] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.80
8
 starch-branching enzyme 1 (Sbe1;Rbe1;BEI;OsBEI;Os06g0726400) 2.4.1.18 Oryza sativa Japonica Group BAF20543.1
AAD28284.1
AAP68993.1
AAU99032.1
AAX83629.1
ABA16988.1
ABD15942.1
ABD15943.1
ABD15944.1
ABD15945.1
ABE12599.1
ABL74557.1
ABL74558.1
ACM95329.1
ACY56114.1
ADK61026.1
ADK61027.1
AEB33747.1
AEB33748.1
AEB33749.1
AEB33750.1
AEB33751.1
AEB33752.1
AEB33753.1
AEB33754.1
AHL84201.1
AHL84202.1
AHL84212.1
AII21924.1
BAA01584.1
BAA01616.1
BAA01855.1
BAD61712.1
BAD61713.1
BAD61804.1
BAD61805.1
BAG89369.1
BAS99598.1
BAS99599.1
BAS99600.1
Q01401
Q0D9D0
Q7XZP9
3AMK[A] 1.90
3AML[A] 1.70
3VU2[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
β-D-Glcp
2.23
8
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