Glycoside Hydrolase Family 13 / Subf 7

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (143); Uniprot accession (28); PDB accession (4); 3D entries (2); cryst (0)
Archaea
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 α-amylase (thermostable)   Pyrococcus woesei    
3QGV[A] α-D-Glcp-(1-2)-β-D-Fruf
b-cyclodextrin
2.10
7
 α-amylase (AmyA) 3.2.1.1 Pyrococcus woesei AAD54338.1
AAF44693.1
ACH26211.1
Q7LYT7
Q9P9L0
1MWO[A] 2.20
1MXD[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp
α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp
α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp
2.00
1MXG[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.60
7
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