Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (277); Uniprot accession (16); PDB accession (4); 3D entries (2); cryst (0)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
PAP_00275		Palaeococcus pacificus DY20341	AIF68502.1			7
PFC_01500		Pyrococcus furiosus COM1	AFN03271.1			7
PFDSM3638_02375		Pyrococcus furiosus DSM 3638	QEK78190.1			7
α-amylase (AmyA; PFA; PfAmy; PF0477)	3.2.1.1	Pyrococcus furiosus DSM 3638	NP_578206.1 AAB67705.1 AAC45663.1 AAL80601.1	O08452 Q8U3I9		7
α-amylase (thermostable)		Pyrococcus woesei			3QGV[A]	7
α-amylase (AmyA)	3.2.1.1	Pyrococcus woesei	AAD54338.1 AAF44693.1 ACH26211.1	Q7LYT7 Q9P9L0	1MWO[A] 1MXD[A] 1MXG[A]	7
PYCH_18550		Pyrococcus yayanosii CH1	AEH25510.1			7
FPV09_08980		Thermococcus aciditolerans SY113	QEK15204.1			7
A3L01_09160		Thermococcus barossii SHCK-94	ASJ05521.1			7
TIRI35C_2170		Thermococcus camini IRI35c	CAD5245324.1			7
A3L02_07325 (fragment)		Thermococcus celer Vu 13	ASI99378.1			7
CL1_1040		Thermococcus cleftensis	AFL95243.1			7
α-amylase (TeAmy; TEU_02420)	3.2.1.1	Thermococcus eurythermalis A501	AIU69287.1 WP_050002265.1			7
TGAM_0222 (AmyA-1)		Thermococcus gammatolerans EJ3	ACS32724.1			7
X802_03495 (fragment)		Thermococcus guaymasensis DSM 11113	AJC72690.1			7
α-amylase (Amy)	3.2.1.1	Thermococcus hydrothermalis AL662	AAC97877.1 AAC97878.1 ACH26213.1	O93647 O93648		7
α-amylase (ApkA;TK1884)	3.2.1.1	Thermococcus kodakarensis KOD1	BAA21130.1 ACH26210.1 BAD86073.1 CAT70635.1	O33476 Q5JER7		7
POG15_09595		Thermococcus kodakarensis TS900	WCN27785.1			7
POG21_09580		Thermococcus kodakarensis TS901	WCN30081.1			7
α-amylase (TO_Amyl;TON_1660)	3.2.1.1	Thermococcus onnurineus NA1	ABA26948.1 ACJ17150.1	B6YUG5 Q2QC88		7
A0127_05805		Thermococcus peptonophilus OG-1	AMQ18717.1			7
A7C91_03825 (fragment)		Thermococcus piezophilus CDGS	ANF22396.1			7
A3L09_01615		Thermococcus profundus DT 5432	ASJ02053.1			7
A3L11_02635		Thermococcus siculi RG-20	ASJ08179.1			7
ADU37_CDS21030		Thermococcus sp. 2319x1	ALV63800.1			7
GQS_07090		Thermococcus sp. 4557	AEK73316.1			7
α-amylase (BD5031)	3.2.1.1	Thermococcus sp. AEPII 1a	AAM48113.1 ACH26099.1	Q8NKR4		7
TAM4_1203		Thermococcus sp. AM4	EEB73455.2			7
α-amylase (BD5064)	3.2.1.1	Thermococcus sp. GU5L5	AAM48112.1 ACH26098.1	Q8NKR5		7
α-amylase (THJA)	3.2.1.1	Thermococcus sp. HJ21	ABU98335.1	B4X9V8		7
A3L12_00010		Thermococcus sp. P6	ASJ09798.1			7
α-amylase	3.2.1.1	Thermococcus sp. Rt3	AAB87860.1 ACH26212.1	O50200		7
α-amylase (Amy)	3.2.1.1	Thermococcus thioreducens	AAT11125.1	Q66SB4		7
A3L14_00190		Thermococcus thioreducens OGL-20P	ASJ11397.1			7
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
MQE35_14105		Abyssalbus ytuae MT3330	UOB16864.1			7
FGM00_09895		Aggregatimonas sangjinii F202Z8	QCX00409.1			7
FK178_01260		Antarcticibacterium arcticum PAMC 28998	QED36430.1			7
FHG64_04555		Antarcticibacterium flavum KCTC 52984	QCY68724.1			7
D1815_22575		Aquimarina sp. AD1	AXT58784.1			7
D1816_00085		Aquimarina sp. AD10	AXT58812.1			7
D1816_10195		Aquimarina sp. AD10	AXT60703.1			7
D1818_08500		Aquimarina sp. BL5	AXT50860.1			7
AUTU_14950		Aureibacter tunicatorum NBRC 107587	BDD04012.1			7
B9G69_010600		Bdellovibrio sp. SKB1291214	UYL07493.1			7
BREVNS_1670		Brevinematales bacterium NS	QJR22420.1			7
BREVNS_2269		Brevinematales bacterium NS	QJR23019.1			7
BREVNS_1294		Brevinematales bacterium NS	QJR22044.1			7
CaldiYA01_19950		Caldicellulosiruptor diazotrophicus YA01	BCS82035.1			7
Calkro_2177		Caldicellulosiruptor kronotskyensis 2002	ADQ47015.1			7
Calow_0294		Caldicellulosiruptor owensensis OL	ADQ03895.1			7
CGC47_06505		Capnocytophaga canimorsus 7120	ATA77254.1			7
Ccan_22280 (Bla)		Capnocytophaga canimorsus Cc5	AEK24343.1			7
CGC54_06485		Capnocytophaga canimorsus H3936	ATA94000.1			7
CGC56_06725		Capnocytophaga canimorsus H5594	ATA91886.1			7
D8L92_08505		Capnocytophaga canimorsus RBWH	AYW37327.1			7
DKB58_07075		Capnocytophaga canimorsus Rmd13-Crie	AWL78717.1			7
AXF12_02095		Capnocytophaga haemolytica CCUG 32990	AMD84430.1			7
SAMEA44541418_01332 (AmyS)		Capnocytophaga haemolytica NCTC12947	SNV10595.1			7
CGC53_04725		Capnocytophaga leadbetteri H6253	ATA81701.1			7
Coch_0631		Capnocytophaga ochracea DSM 7271	ACU92189.1			7
OLG90_00645		Capnocytophaga ochracea KCOM 2191	UZD36432.1			7
OL230_02985		Capnocytophaga ochracea KCOM 26681	UZD39147.1			7
OL231_08330		Capnocytophaga ochracea KCOM 28121	UZD40185.1			7
CBG49_00375		Capnocytophaga sp. ChDC OS43	ASF41659.1			7
FOC45_10550		Capnocytophaga sp. FDAARGOS_737	QGS18688.1			7
CGC52_05680		Capnocytophaga sp. H2931	ATA74953.1			7
CGC49_05925		Capnocytophaga sp. H4358	ATA72859.1			7
AM608_06725		Capnocytophaga sp. oral taxon 323 str. F0383	ALC97351.1			7
C3V44_09080		Capnocytophaga sp. oral taxon 864 F0512	AVM55753.1			7
C4H12_12130		Capnocytophaga sp. oral taxon 878 F0545	AVM51143.1			7
HW278_04505		Capnocytophaga sp. oral taxon 902 W10638	QLF50014.1			7
Ctha_2426		Chloroherpeton thalassium ATCC 35110	ACF14875.1			7
GRFL_0201		Christiangramia flava JLT2011	APU66925.1			7
GRFL_2435		Christiangramia flava JLT2011	APU69159.1			7
orf2122		Christiangramia forsetii KT0803	CAL67106.1			7
C7S20_17985		Christiangramia fulva SH35	AVR47549.1			7
MKO06_15420		Gramella oceanisediminis GC03-9	UNG10390.1			7
MKO06_13655		Gramella oceanisediminis GC03-9	UNG10885.1			7
LPB144_01590		Christiangramia salexigens LPB0144	APG59177.1			7
LPB144_06405		Christiangramia salexigens LPB0144	APG60068.1			7
T8I65_05200		Christiangramia sp. OXR-203	WPY99603.1			7
EGY05_17260		Chryseobacterium arthrosphaerae FDAARGOS_519	AYZ13580.1			7
P2W68_11845		Chryseobacterium arthrosphaerae FS91703	WET00301.1			7
J8N07_11465		Chryseobacterium arthrosphaerae LMY	UEQ78883.1			7
I2F65_16160		Chryseobacterium arthrosphaerae SQ099	QUY54407.1			7
EG339_00650		Chryseobacterium bernardetii G0229	AZB23226.1			7
EG351_10520		Chryseobacterium bernardetii H4638	AZB34008.1			7
H5J24_02515		Chryseobacterium capnotolerans DH-B6	UHO39052.1			7
EG345_01920		Chryseobacterium carnipullorum F9942	AZA63594.1			7
EG346_11020		Chryseobacterium carnipullorum G0188	AZA48680.1			7
JNG87_00960		Chryseobacterium cucumeris MW-6	QRA43356.1			7
MUU74_08755		Chryseobacterium daecheongense F23	UOV00031.1			7
PFY10_09395		Chryseobacterium daecheongense PL22-22A	WBV58660.1			7
ODZ84_09955		Chryseobacterium fluminis MMS21-Ot14	UZT99854.1			7
OK18_03240		Chryseobacterium gallinarum DSM 27622	AKK71784.1			7
FOB44_18270		Chryseobacterium gallinarum FDAARGOS_636	QIY92477.1			7
PE065_05740		Chryseobacterium gambrini DMF-6	WBX98755.1			7
CRDW_16750		Chryseobacterium gambrini DW100	BEV04301.1			7
PFY09_08595		Chryseobacterium gambrini PL22-1A	WBV54368.1			7
DDI74_09770		Chryseobacterium gleum 110146	QBJ86529.1			7
NCTC12078_01127 (Amys_1)		Chryseobacterium gleum 3012STDY6944375	VFB03138.1			7
I6I60_08365		Chryseobacterium gleum FDAARGOS_1103	QQY33764.1			7
NCTC11432_02467 (Amys_2)		Chryseobacterium gleum NCTC11432	VEE08068.1			7
NCTC11409_02099 (Amys_1)		Chryseobacterium indologenes 3012STDY6981895	VFA42089.1			7
JHW31_10120		Chryseobacterium indologenes 3125	QQQ73054.1			7
LJF28_07365		Chryseobacterium indologenes 742	UDQ55480.1			7
H3Z85_03525		Chryseobacterium indologenes DSM 16777	QPQ52554.1			7
PYS58_11520		Chryseobacterium indologenes Ery-6B	WET51749.1			7
CEQ15_14350		Chryseobacterium indologenes FDAARGOS_337	ASE62591.1			7
CRN76_13870		Chryseobacterium indologenes FDAARGOS_379	ATN06411.1			7
EGY07_02505		Chryseobacterium indologenes FDAARGOS_510	AYZ34515.1			7
EGX91_09855		Chryseobacterium indologenes FDAARGOS_537	AYY84828.1			7
FOB56_10910		Chryseobacterium indologenes FDAARGOS_648	QIX81713.1			7
EG352_11110		Chryseobacterium indologenes H5559	AZB18286.1			7
EU348_05750		Chryseobacterium indologenes StR 01	QBA20716.1			7
EG359_10485		Chryseobacterium joostei DSM 16927	AZB00024.1			7
EG341_17055		Chryseobacterium lactis G0197	AZB05567.1			7
EG342_00930		Chryseobacterium lactis KC_1864	AZA80565.1			7
EG343_09220		Chryseobacterium nakagawai G0041	AZA90793.1			7
NCTC13529_04436 (Amys_1)		Chryseobacterium nakagawai NCTC13529	VEH22325.1			7
M0D58_14935		Chryseobacterium nepalense AC3	UPQ75332.1			7
N0B40_19850		Chryseobacterium oranimense 33-M-3	UWX60628.1			7
CJF12_18350		Chryseobacterium piperi ATCC BAA-1782	ASW76037.1			7
KB553_11105		Chryseobacterium rhizoplanae XL97	UCA62040.1			7
EG349_08280		Chryseobacterium shandongense G0207	AZA86784.1			7
EG350_15760		Chryseobacterium shandongense G0239	AZA58549.1			7
EG353_06300		Chryseobacterium shandongense H5143	AZA95197.1			7
PGH12_08810		Chryseobacterium sp. CY350	WBZ97235.1			7
KYG33_10665		Chryseobacterium sp. D764	QXU51472.1			7
I6I61_04110		Chryseobacterium sp. FDAARGOS 1104	QQV03531.1			7
EG344_15645		Chryseobacterium sp. G0162	AZB10147.1			7
EG347_09985		Chryseobacterium sp. G0186	AZA77824.1			7
EG348_02660		Chryseobacterium sp. G0201	AZA51983.1			7
SFA27_04245		Chryseobacterium sp. HR92	WPO91895.1			7
ATE47_11975		Chryseobacterium sp. IHB B 17019	ALR31194.1			7
SD427_15270		Chryseobacterium sp. JJR-5R	WPO82116.1			7
CHRYMOREF3P_2039		Chryseobacterium sp. JV274 F3_Prior	CAD0221046.1			7
K0U91_09785		Chryseobacterium sp. LJ668	QYK15363.1			7
KIK00_11175		Chryseobacterium sp. MA9	UTX50787.1			7
LF887_19445		Chryseobacterium sp. MEBOG06	UKB83165.1			7
LF886_05855		Chryseobacterium sp. MEBOG07	UKB80516.1			7
KBP46_19280		Chryseobacterium sp. PCH239	QWT85565.1			7
RHP76_10345		Chryseobacterium sp. SG20098	WNI38875.1			7
CHSO_4148		Chryseobacterium sp. StRB126	BAP33185.1			7
CEY12_10440		Chryseobacterium sp. T16E-39	ASK30499.1			7
PZ898_10630		Chryseobacterium sp. WX	WFB69871.1			7
KKI44_09390		Chryseobacterium sp. ZHDP1	QWA40389.1			7
CA2559_00080		Croceibacter atlanticus HTCC2559	EAP87105.1			7
CYPRO_2872		Cyclonatronum proteinivorum Omega	AXJ02110.1			7
GWR55_05380		Edaphobacter sp. 12200R-103	QHS51230.1			7
HME9304_02348		Flagellimonas maritima HME9304	AWX45335.1			7
LV716_02850 (fragment)		Flagellimonas sp. HMM57	UII76745.1			7
LV716_02855 (fragment)		Flagellimonas sp. HMM57	UII76746.1			7
α-amylase, partial (Amy16)		Flammeovirga pacifica WPAGA1	AKJ26156.1			7
MY04_3248		Flammeovirga sp. MY04	ANQ50613.2 ANQ50613.1			7
KMW28_20510		Flammeovirga yaeyamensis NBRC:100898	QWG01982.1			7
C1H87_05890		Flavivirga eckloniae ECD14	AUP78269.1			7
α-amylase (FSA)	3.2.1.1	Flavobacteriaceae bacterium BPA / 5DNS001	AGD88873.1			7
UJ101_02334		Flavobacteriaceae bacterium UJ101	APD07834.1			7
UJ101_01162		Flavobacteriaceae bacterium UJ101	APD06687.1			7
FUA48_00505		Flavobacterium alkalisoli XS-5	QEE48109.1			7
LLY39_09385		Flavobacterium anhuiense T4	URM38716.1			7
DVK85_00800		Flavobacterium arcticum SM1502	AXG72845.1			7
L0669_10265		Flavobacterium bizetiae HJ-32-4	UTN06280.1			7
TRV642_2106 (AmyS)		Flavobacterium collinsii TRV642	CAI2767020.1			7
J0383_05795		Flavobacterium endoglycinae BB8	QSW90327.1			7
HYN86_09330		Flavobacterium fluviale HYN0086	AXB56788.1			7
V6624_04775		Flavobacterium ginsenosidimutans YTB16	WXK50947.1			7
M0M44_10140		Flavobacterium humidisoli MMS21-Er5	UPZ17692.1			7
NYQ10_09300		Flavobacterium johnsoniae G16	WJS96639.1			7
BB050_00652 (AmyS)		Flavobacterium johnsoniae GSE09	AOC93801.1			7
Fjoh_1948		Flavobacterium johnsoniae UW101	ABQ04980.1			7
SR927_08910		Flavobacterium johnsoniae UW101 ATCC 17061	WQG83222.1			7
K1I41_00785		Flavobacterium litorale WSW3-B6	QYJ68450.1			7
NLJ00_10610		Flavobacterium panici BSSL-CR3	UUF16550.1			7
Q1W71_22485		Flavobacterium pectinovorum ZE23VCel01	WKL47707.1			7
ALW18_06450		Flavobacterium psychrophilum Z1	AOE52185.1			7
AMR72_06460		Flavobacterium psychrophilum Z2	ALM48568.1			7
LXD69_11860		Flavobacterium sediminilitoris YSM-43	UOX32735.1			7
AABD74_10020		Flavobacterium soyae 55	WYZ21784.1			7
EAG11_00970		Flavobacterium sp. 140616W15	AYN02899.1			7
FK004_00180 (fragment)		Flavobacterium sp. AJ004	AWG23753.1			7
FK004_00190 (fragment)		Flavobacterium sp. AJ004	AWG23755.1			7
M4I44_10120		Flavobacterium sp. B183	URC14721.1			7
FVB9288_02461 (AmyS)		Flavobacterium sp. CECT 9288	CAH0336748.1			7
K5V07_11780		Flavobacterium sp. CHNK8	QZK91135.1			7
HAV12_018805		Flavobacterium sp. CLA17	QSB26399.1			7
LNP23_09940		Flavobacterium sp. F-323	UFH44519.1			7
LNP22_09640		Flavobacterium sp. F-70	UFH37000.1			7
FLGSB24_17770		Flavobacterium sp. GSB-24	BDU25033.1			7
FLAK523_00210		Flavobacterium sp. K5-23	UQD54889.1			7
PQ462_04870		Flavobacterium sp. KACC 22758	WDF60698.1			7
SCB73_11775		Flavobacterium sp. KACC 22761	WPO76947.1			7
PQ463_22850		Flavobacterium sp. KACC 22763	WDF64438.1			7
B0M43_0010040		Flavobacterium sp. KBS0721	QDW20432.1			7
V5J73_03165		Flavobacterium sp. KS-LB2	WWP41662.1			7
LPB248_05965		Flavobacterium sp. LPB0248 LPB248	QLC65848.1			7
HQN62_06545		Flavobacterium sp. M31R6	QKJ62802.1			7
IHE43_20925		Flavobacterium sp. MDT1-60	QOG02220.1			7
GIY83_14910		Flavobacterium sp. SLB02 SGTM	QGK75319.1			7
G7A72_05920		Flavobacterium sp. Sr18	QIH38365.1			7
N4T20_09980		Flavobacterium sp. TR2	UWY30248.1			7
P0R33_07960		Flavobacterium sp. YJ01	WET04261.1			7
Q1W72_12405		Flavobacterium sp. ZE23DGlu08	WKL43146.1			7
FUAX_44700		Fulvitalea axinellae DSM 100852	BDD12038.1			7
FUAX_45970		Fulvitalea axinellae DSM 100852	BDD12165.1			7
LVD16_15795		Fulvivirga ligni W9P-11	UII19307.1			7
LVD15_22470		Fulvivirga maritima SW1-E11	UII26040.1			7
LVD17_21290		Fulvivirga ulvae SS9-22	UII30831.1			7
HX109_04315		Galbibacter sp. bg1	QLE00826.1			7
SAMN04487764_3183		Gillisia sp. Hel1_33_143	SDS83390.1			7
SAMN04488553_0218		Gramella sp. MAR_2010_147	SDR67666.1			7
SAMN04488553_2877		Gramella sp. MAR_2010_147	SDS69681.1			7
GOB94_09235		Granulicella sp. 5B5	QMV18844.1			7
FTO74_04920		Granulicella sp. WH15	QHN02785.1			7
AciX9_1331		Granulicella tundricola MP5ACTX9	ADW68393.1			7
Hore_18730		Halothermothrix orenii H 168	ACL70622.1			7
IMCC3317_41410		Kordia antarctica IMCC3317	QHI38747.1			7
KORDIASMS9_02351		Kordia sp. SMS9	AXG70122.1			7
Lbys_2308		Leadbetterella byssophila DSM 17132	ADQ17984.1			7
LDL79_06065		Leeuwenhoekiella palythoae F5	UBZ11678.1			7
Lupro_04465		Lutibacter profundi LP1	AMC10538.1			7
Q3Y49_15145		Marivirga harenae KCTC 92433	WKV11538.1			7
QYS49_37335		Marivirga sp. BDSF4-3	WMN11138.1			7
Ftrac_0472		Marivirga tractuosa DSM 4126	ADR20477.1			7
MATR_19020		Marivirga tractuosa NBRC 15989	BDD15077.1			7
IVG45_09845		Methylomonas sp. LL1	QPK65205.1			7
MgSA37_01758		Mucilaginibacter gotjawali SA3-7	BAU53589.1			7
EJ995_05150		Nonlabens ponticola MJ115	AZQ43647.1			7
DZC78_14095		Olleya aquimaris DAU311	AXO81475.1			7
CW732_07600		Olleya sp. Bg11-27	AUC75545.1			7
H0I26_07330		Olleya sp. HaHaR_3_96	QXP61440.1			7
AY601_1910		Pedobacter cryoconitis PAMC 27485	AMP98817.1			7
H7F33_08995		Pedobacter sp. PAMC26386	QNK64933.1			7
PBC10988_32510		Planctomycetales bacterium 10988	QGJ71544.1			7
RJD25_00865		Pontibacter sp. G13	WNJ19014.1			7
E9099_02550		Psychroserpens sp. NJDZ02	QCE40341.1			7
RHOA_4443		Rhodovastum atsumiense G2-11	CAH2603154.1			7
DCC35_20285		Roseivirga pacifica 9dcg1	QCK16905.1			7
LDL76_16595		Salegentibacter mishustinae F1	UBZ06963.1			7
AO058_10120		Salegentibacter sp. T436	APS39205.1			7
JRG66_00430		Salinimicrobium tongyeongense HN-2-9-2	UZH55406.1			7
LS482_04205		Sinomicrobium kalidii HD2P242	UGU17076.1			7
LS482_13810		Sinomicrobium kalidii HD2P242	UGU14768.1			7
SAMN06298216_0723		Spirosomataceae bacterium TFI 002	SOE20227.1			7
TJEJU_1257 (SusA)		Tenacibaculum jejuense KCTC 22618	SNR14997.1			7
BTO06_03440		Tenacibaculum sp. SZ-18	AUC16998.1			7
FTW19_17995		Terriglobus albidus ORNL	QEE29707.1			7
Terro_1447		Terriglobus roseus DSM 18391	AFL87755.1			7
SAMN05444167_1973		Terriglobus roseus GAS232	SDF29322.1			7
AciPR4_2183		Terriglobus saanensis SP1PR4	ADV82985.1			7
KDW03_05505		Thermospira aquatica F1F22	URA11253.1			7
KDW03_03995		Thermospira aquatica F1F22	URA10974.1			7
α-amylase, glycoside hydrolase family GH13-7		uncultured bacterium	AOE07240.1			7
MQE36_07220		Zhouia spongiae HN-Y44	UNZ00126.1			7
zobellia_369 (SusA)		Zobellia galactanivorans DsijT	CAZ94442.1			7
α-amylase (AmyZ2;ZPR_2825)	3.2.1.1	Zunongwangia profunda SM-A87	ADF53145.1			7
PBT91_06195		Zunongwangia sp. HGR-M22	WBL26852.1			7
PBT89_11145		Zunongwangia sp. HRR-M8	WBL21290.1			7
INR75_17240		Zunongwangia sp. SCSIO 43204	UAB83896.1			7
unclassified
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
α-amylase (BD5063)	3.2.1.1	uncultured organism	AAM48114.1 ACH26097.1	Q8JZK3		7
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