Glycoside Hydrolase Family 13 / Subf 5

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (2341); Uniprot accession (238); PDB accession (24); 3D entries (8); cryst (0)
Eukaryota
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 EGX46868.1   Arthrobotrys oligospora ATCC 24927 EGX46868.1     5
 AN3309.2 (AmyG)   Aspergillus nidulans FGSC A4 EAA63277.1 C8VHZ2
Q5B821
  5
 maltotriose-producing α-amylase (AmyD;An01g13610) (intracellular) 3.2.1.1
3.2.1.116
Aspergillus niger CBS 513.88 CAK37367.1 A2QB23   5
 An09g03110 (AmyE)   Aspergillus niger CBS 513.88 CAK40250.1 A2QTS5   5
 ORF   Aspergillus oryzae BAC55903.1 Q877B1   5
 AO090005001193   Aspergillus oryzae RIB40 BAE56147.1 Q2UQL8   5
 AO090003001497   Aspergillus oryzae RIB40 BAE58539.1 Q2UIS6   5
 glycoside hydrolase family 13 protein, partial sequence (Bofut4_p011850.1) (fragment)   Botrytis cinerea T4 CCD43509.1     5
 glycoside hydrolase family 13 protein, partial sequence (Bofut4_p011860.1) (fragment)   Botrytis cinerea T4 CCD43510.1     5
 CGB_G0540W   Cryptococcus gattii WM276 ADV23254.1     5
 CNAG_03146   Cryptococcus neoformans var. grubii H99 AFR96372.1
AFR96372.2
    5
 CNG04200   Cryptococcus neoformans var. neoformans JEC21 AAW44866.1
AAW44867.1
Q5KDF2   5
 α-amylase   Flammulina velutipes L11 AOC97452.1     5
 α-amylase   Flammulina velutipes L11 AOC97453.1     5
 α-amylase (AmyPI12)   Glaciozyma antarctica PI12 AFX74688.1     5
 a-(1,3)-glucan-related enzyme (Amy1)   Histoplasma capsulatum G186A / ATCC 26029 ABK62854.1 A0T074   5
 ORF   Hypocrella siamensis MTCC 10142 ANH56422.1     5
 ORF   Leptosphaeria maculans JN3 v23.1.3 CBX99625.1 E5A7N0   5
 MG03287.4   Magnaporthe oryzae 70-15 XP_360744.1 G4N9G3   5
 MGG_09642   Magnaporthe oryzae 70-15 XP_003721441.1
XP_364797.1
    5
 MGCH7_ch7g964   Magnaporthe oryzae 70-15 EAQ71557.1
XP_364319.1
Q2KES2   5
 BN887_03272   Melanopsichium pennsylvanicum 4 CDI56403.1     5
 NCU09486.1 (B22K18.090)   Neurospora crassa OR74A CAE75731.1
XP_329845.1
    5
 NCU05873.1   Neurospora crassa OR74A XP_325728.1     5
 cell-wall active enzyme / α-amylase (Amy1;PbrAmy1) 2.4.1.-
3.2.1.1
Paracoccidioides brasiliensis IVIC Pb73 / ATCC 3207 ABS11196.1 A7L832   5
 Pc06g01090   Penicillium rubens Wisconsin 54-1255 CAP79102.1 B6GW48   5
 Pc16g06150   Penicillium rubens Wisconsin 54-1255 CAP93285.1 B6H8T1   5
 α-amylase (Pnamy2)   Pholiota microspora BAW15172.1     5
 ORF   Podospora anserina S mat+ CDP29184.1
CAP62108.1
B2AF91   5
 unnamed protein product   Podospora anserina S mat+ CAP70916.1
CDP27512.1
B2B192   5
 RHTO0S_06e01926g   Rhodotorula toruloides CECT1137 CDR41446.1     5
 sscle_16g107710   Sclerotinia sclerotiorum 1980 UF-70 APA16001.1     5
 PIIN_00328   Serendipita indica DSM 11827 CCA66645.1     5
 PIIN_02181   Serendipita indica DSM 11827 CCA68317.1     5
 sr13497   Sporisorium reilianum SRZ2 CBQ71030.1     5
 SPSC_01712   Sporisorium scitamineum SscI8 CDU23082.1     5
 MYCTH_69975   Thermothelomyces thermophila ATCC 42464 AEO58507.1     5
 UBRO_03784   Ustilago bromivora UB2112 SAM62792.1     5
 α-amylase   Xanthophyllomyces dendrorhous CDZ98132.1     5
 ZT1A5_G3343   Zymoseptoria tritici ST99CH_1A5 SMY21905.1     5
 ZT1A5_G2282   Zymoseptoria tritici ST99CH_1A5 SMY20846.1     5
 ZT3D7_G3362   Zymoseptoria tritici ST99CH_3D7 SMQ48213.1     5
 ZT3D7_G2315   Zymoseptoria tritici ST99CH_3D7 SMQ47168.1     5
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