Glycoside Hydrolase Family 13 / Subf 5

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (2426); Uniprot accession (238); PDB accession (24); 3D entries (8); cryst (0)
| 1 | 2 | 3 |
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 SMQ301_1526   Streptococcus thermophilus SMQ-301 AKB98146.1     5
 BGL51_07475   Streptococcus thermophilus ST3 ASX19774.1     5
 SRT_05300 (AmyA)   Streptococcus troglodytae TKU 31 BAQ23791.1     5
 SUB0537   Streptococcus uberis 0140J CAR41297.1 B9DU10   5
 BM449_05095   Synechococcus sp. SynAce01 APD47752.1     5
 N840_1967 (AmyA)   synthetic Escherichia coli C321.deltaA AGX34006.1     5
 A7K99_03600   Tatumella citrea ATCC 39140 ARU97000.1     5
 A7K98_03600   Tatumella citrea DSM 13699 ARU92961.1     5
 AC806_03625   Tetragenococcus halophilus MJ4 AOF48556.1     5
 TEH_10730 (AmY)   Tetragenococcus halophilus NBRC 12172 BAK94400.1     5
 NIES37_18620   Tolypothrix tenuis PCC 7101 NIES-37 BAY97914.1     5
 NIES23_05950   Trichormus variabilis NIES-23 BAY67813.1     5
 alpha amylase, catalytic domain protein, partial (fragment)   uncultured bacterium AMJ35464.1     5
 alpha amylase, catalytic domain protein, partial (fragment)   uncultured bacterium AMJ35403.1     5
 alpha amylase, catalytic domain protein, partial (fragment)   uncultured bacterium AMJ35826.1     5
 α-amylase   uncultured bacterium ACY08858.1 D0V5E8   5
 α-amylase (Amy13A) 3.2.1.1 uncultured bacterium CCD30600.1     5
 pKSBS-4 (probable fragment)   uncultured bacterium ABW94981.1 A8WE99   5
 ORF (fragment)   uncultured bacterium Contig1209 AHF26915.1     5
 K04M3_39910 (AmyS)   Vibrio alginolyticus ARP10581.1     5
 α-amylase (Amy63) 3.2.1.1 Vibrio alginolyticus 129-63 ALP73597.1     5
 AT730_17320   Vibrio alginolyticus ATCC 33787 ALR94094.1     5
 K01M1_39960 (AmyS)   Vibrio alginolyticus K01M1 ARP00823.1     5
 K04M1_39890 (AmyS)   Vibrio alginolyticus K04M1 ARP05523.1     5
 K04M5_40170 (AmyS)   Vibrio alginolyticus K04M5 ARP15680.1     5
 K05K4_40120 (AmyS)   Vibrio alginolyticus K05K4 ARP20734.1     5
 K06K5_39860 (AmyS)   Vibrio alginolyticus K06K5 ARP25807.1     5
 K08M3_39810 (AmyS)   Vibrio alginolyticus K08M3 ARP35607.1     5
 K09K1_15940 (AmyS)   Vibrio alginolyticus K09K1 ARP46232.1     5
 K10K4_40040 (AmyS)   Vibrio alginolyticus K10K4 ARP30876.1     5
 N646_4374   Vibrio alginolyticus NBRC 15630 = ATCC 17749 AGV20183.1     5
 BAU10_20080   Vibrio alginolyticus ZJ-T ANP67253.1     5
 VEA_000056   Vibrio antiquarius EX25 ACY52744.1 D0MCN0   5
 Vca1114GL_04249 (AmyS)   Vibrio campbellii 1114GL AQM70670.1     5
 Vc3S01_A1185 (AmyA)   Vibrio campbellii 20130629003S01 ARR09158.1     5
 VIBHAR_05679   Vibrio campbellii ATCC BAA-1116 ABU73577.1
AGU97592.1
A7N404   5
 A8140_17165   Vibrio campbellii CAIM 519 = NBRC 15631 ATCC 25920, CAIM 519T ARV74389.1     5
 CAY59_17425   Vibrio campbellii LA16-V1 ARR46080.1     5
 BWP24_20870   Vibrio campbellii LMB29 APX08635.1     5
 BG259_20695   Vibrio harveyi APP07693.1     5
 LA59_26690   Vibrio harveyi ATCC 33843 (392 [MAV]) AIV08970.1     5
 AL538_24695   Vibrio harveyi ATCC 43516 AMG00871.1     5
 A9237_21540   Vibrio owensii XSBZ03 AQW60604.1     5
 BMI84_19320   Vibrio parahaemolyticus 1682 APU76149.1     5
 AL464_16925   Vibrio parahaemolyticus ATCC 17802 AMG08491.1     5
 VPBB_A0909   Vibrio parahaemolyticus BB22OP AGB12283.1     5
 VpaChn25_A0928   Vibrio parahaemolyticus CHN25 ANZ12514.1     5
 A6J30_23160   Vibrio parahaemolyticus FDAARGOS_191 ARC21322.1     5
 FORC4_3841   Vibrio parahaemolyticus FORC_004 ALM68814.1     5
 FORC6_3915   Vibrio parahaemolyticus FORC_006 ALG54241.1     5
 FORC8_3841   Vibrio parahaemolyticus FORC_008 AKU57401.1     5
 FORC14_4028   Vibrio parahaemolyticus FORC_014 ANB99724.1     5
 FORC18_3848   Vibrio parahaemolyticus FORC_018 APE86461.1     5
 FORC22_4124   Vibrio parahaemolyticus FORC_022 APC89985.1     5
 FORC23_3703   Vibrio parahaemolyticus FORC_023 AOV92246.1     5
 CO725_01875   Vibrio parahaemolyticus HA2 ATI44430.1     5
 YA91_04695   Vibrio parahaemolyticus MAVP-26 ASZ49913.1     5
 BGM07_004860   Vibrio parahaemolyticus MAVP-Q ASO13744.1     5
 CHR23_019350   Vibrio parahaemolyticus MAVP-R ASQ37952.1     5
 M636_04380   Vibrio parahaemolyticus O1:K33 str. CDC_K4557 AGQ96553.1     5
 M634_22905   Vibrio parahaemolyticus O1:Kuk str. FDA_R31 AGQ93587.1     5
 VPA0999   Vibrio parahaemolyticus RIMD 2210633 O3:K6 BAC62342.1
ACH26143.1
ACH26147.1
ACH26148.1
NP_800509.1
    5
 AB831_20545   Vibrio parahaemolyticus ST631 ANQ58558.1     5
 VPUCM_20884   Vibrio parahaemolyticus UCM-V493 AHJ02005.1     5
 BSZ04_08005   Vibrio rotiferianus B64D1 ASI94941.1     5
 BSZ04_02515   Vibrio rotiferianus B64D1 ASI93912.1     5
 BSZ05_23315   Vibrio shilonii QT6D1 ASI92705.1     5
 AT01_136   Yersinia aldovae 670-83 AJJ63145.1     5
 ACZ76_00750   Yersinia aleksiciae 159 AKP32184.1     5
 YE5303_22781 (AmyA)   Yersinia enterocolitica (type O:5) str. YE53/03 CCQ40982.1     5
 CH48_3382   Yersinia enterocolitica 2516-87 AJJ27615.1     5
 CH49_1398   Yersinia enterocolitica 8081 AJJ22616.1     5
 LI89_08665   Yersinia enterocolitica FORC-002 ALG44798.1     5
 FORC2_2474   Yersinia enterocolitica FORC_002 AKF38621.1     5
 XM56_08275   Yersinia enterocolitica KNG22703 ALG78414.1     5
 LC20_02230 (AmyA)   Yersinia enterocolitica LC20 AHM73483.1     5
 YE1962   Yersinia enterocolitica subsp. enterocolitica 8081 CAL12041.1 A1JN22   5
 YE105_C1767   Yersinia enterocolitica subsp. palearctica 105.5R(r) ADZ42263.1     5
 Y11_16360   Yersinia enterocolitica subsp. palearctica Y11 CBY26822.1     5
 YEW_LK48500 (AmyA) (fragment)   Yersinia enterocolitica W22703 CBX70854.1     5
 CH47_1400   Yersinia enterocolitica WA AJI81434.1     5
 BFS78_09525   Yersinia enterocolitica YE1 AOF35160.1     5
 BB936_10085   Yersinia enterocolitica YE165 AOF14771.1     5
 BED35_10455   Yersinia enterocolitica YE3 AOF31241.1     5
 BED33_12665   Yersinia enterocolitica YE6 AOF23419.1     5
 PL78_17905   Yersinia entomophaga MH96 ANI31684.1     5
 CRN74_09330   Yersinia frederiksenii FDAARGOS_417 ATM86261.1     5
 CRN75_13235   Yersinia frederiksenii FDAARGOS_418 ATM96235.1     5
 AW19_930   Yersinia frederiksenii Y225 AJI86924.1     5
 CH53_3615   Yersinia intermedia Y228 AJJ17937.1     5
 DJ57_1580   Yersinia kristensenii ATCC 33639 AIN19048.1     5
 CH54_733   Yersinia kristensenii Y231 AJJ34148.1     5
 CH64_274   Yersinia rohdei YRA AJJ12514.1     5
 A6J67_16270   Yersinia sp. FDAARGOS_228 ARB85384.1     5
Eukaryota
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 EGX46868.1   Arthrobotrys oligospora ATCC 24927 EGX46868.1     5
 AN3309.2 (AmyG)   Aspergillus nidulans FGSC A4 EAA63277.1 C8VHZ2
Q5B821
  5
 maltotriose-producing α-amylase (AmyD;An01g13610) (intracellular) 3.2.1.1
3.2.1.116
Aspergillus niger CBS 513.88 CAK37367.1 A2QB23   5
 An09g03110 (AmyE)   Aspergillus niger CBS 513.88 CAK40250.1 A2QTS5   5
 ORF   Aspergillus oryzae BAC55903.1 Q877B1   5
 AO090005001193   Aspergillus oryzae RIB40 BAE56147.1 Q2UQL8   5
 AO090003001497   Aspergillus oryzae RIB40 BAE58539.1 Q2UIS6   5
 glycoside hydrolase family 13 protein, partial sequence (Bofut4_p011850.1) (fragment)   Botrytis cinerea T4 CCD43509.1     5
 glycoside hydrolase family 13 protein, partial sequence (Bofut4_p011860.1) (fragment)   Botrytis cinerea T4 CCD43510.1     5
 CGB_G0540W   Cryptococcus gattii WM276 ADV23254.1     5
 CNAG_03146   Cryptococcus neoformans var. grubii H99 AFR96372.1
AFR96372.2
    5
 CNG04200   Cryptococcus neoformans var. neoformans JEC21 AAW44866.1
AAW44867.1
Q5KDF2   5
 α-amylase   Flammulina velutipes L11 AOC97452.1     5
 α-amylase   Flammulina velutipes L11 AOC97453.1     5
 α-amylase (AmyPI12)   Glaciozyma antarctica PI12 AFX74688.1     5
 a-(1,3)-glucan-related enzyme (Amy1)   Histoplasma capsulatum G186A / ATCC 26029 ABK62854.1 A0T074   5
 ORF   Hypocrella siamensis MTCC 10142 ANH56422.1     5
 ORF   Leptosphaeria maculans JN3 v23.1.3 CBX99625.1 E5A7N0   5
 MG03287.4   Magnaporthe oryzae 70-15 XP_360744.1 G4N9G3   5
 MGG_09642   Magnaporthe oryzae 70-15 XP_003721441.1
XP_364797.1
    5
 MGCH7_ch7g964   Magnaporthe oryzae 70-15 EAQ71557.1
XP_364319.1
Q2KES2   5
 BN887_03272   Melanopsichium pennsylvanicum 4 CDI56403.1     5
 NCU09486.1 (B22K18.090)   Neurospora crassa OR74A CAE75731.1
XP_329845.1
    5
 NCU05873.1   Neurospora crassa OR74A XP_325728.1     5
 cell-wall active enzyme / α-amylase (Amy1;PbrAmy1) 2.4.1.-
3.2.1.1
Paracoccidioides brasiliensis IVIC Pb73 / ATCC 3207 ABS11196.1 A7L832   5
 Pc06g01090   Penicillium rubens Wisconsin 54-1255 CAP79102.1 B6GW48   5
 Pc16g06150   Penicillium rubens Wisconsin 54-1255 CAP93285.1 B6H8T1   5
 α-amylase (Pnamy2)   Pholiota microspora BAW15172.1     5
 ORF   Podospora anserina S mat+ CDP29184.1
CAP62108.1
B2AF91   5
 unnamed protein product   Podospora anserina S mat+ CAP70916.1
CDP27512.1
B2B192   5
 RHTO0S_06e01926g   Rhodotorula toruloides CECT1137 CDR41446.1     5
 sscle_16g107710   Sclerotinia sclerotiorum 1980 UF-70 APA16001.1     5
 PIIN_00328   Serendipita indica DSM 11827 CCA66645.1     5
 PIIN_02181   Serendipita indica DSM 11827 CCA68317.1     5
 sr13497   Sporisorium reilianum SRZ2 CBQ71030.1     5
 SPSC_01712   Sporisorium scitamineum SscI8 CDU23082.1     5
 MYCTH_69975   Thermothelomyces thermophila ATCC 42464 AEO58507.1     5
 UBRO_03784   Ustilago bromivora UB2112 SAM62792.1     5
 α-amylase   Xanthophyllomyces dendrorhous CDZ98132.1     5
 ZT1A5_G3343   Zymoseptoria tritici ST99CH_1A5 SMY21905.1     5
 ZT1A5_G2282   Zymoseptoria tritici ST99CH_1A5 SMY20846.1     5
 ZT1E4_G2311 (fragment)   Zymoseptoria tritici ST99CH_1E4 SMR45693.1     5
 ZT1E4_G2312 (fragment)   Zymoseptoria tritici ST99CH_1E4 SMR45694.1     5
 ZT1E4_G3380   Zymoseptoria tritici ST99CH_1E4 SMR46762.1     5
 ZT3D1_G2263   Zymoseptoria tritici ST99CH_3D1 SMR46955.1     5
 ZT3D1_G3308   Zymoseptoria tritici ST99CH_3D1 SMR48000.1     5
 ZT3D7_G3362   Zymoseptoria tritici ST99CH_3D7 SMQ48213.1     5
 ZT3D7_G2315   Zymoseptoria tritici ST99CH_3D7 SMQ47168.1     5
unclassified
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 α-amylase   uncultured organism ADD61689.1 D9ZED5   5
 α-amylase   uncultured organism AIB09475.1     5
 Sequence 128 from patent US 7407677.   unidentified ACH26122.1     5
 Sequence 136 from patent US 7407677 (fragment)   unidentified ACH26126.1     5
 Sequence 164 from patent US 7407677.   unidentified ACH26140.1     5
 Sequence 140 from patent US 7407677.   unidentified ACH26128.1     5
 Sequence 146 from patent US 7407677.   unidentified ACH26131.1     5
 Sequence 166 from patent US 7407677. (possible fragment)   unidentified ACH26141.1     5
 Sequence 106 from patent US 7407677. (fragment)   unidentified ACH26111.1     5
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