Glycoside Hydrolase Family 13 / Subf 3

Activities in Sub Family	α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (4); Uniprot accession (5); PDB accession (25); 3D entries (3); cryst (0)

Bacteria

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

Subf

α-1,4-glucan: phosphate α-maltosyltransferase (GlgE;Rv1327c)

CAA98091.2
AFN49234.1
CCP44085.1

4U33[A,B,C,D,E,F]	α-D-Glcp-(1-4)-α-D-Glcp	3.29
4U3C[A,B,C,D,E,F]	α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp	3.98

α-1,4-glucan: phosphate α-maltosyltransferase (GlgE;KEK_12948)

α-1,4-glucan: phosphate α-maltosyltransferase I (GlgE;SCO5443;SC6A11.19C)

CAB72419.1
CAA04600.1
NP_629581.1

3ZSS[A,B,C,D]		1.80
3ZST[A,B]	a-cyclodextrin	2.30
3ZT5[A,B,C,D]	α-D-Glcp-(1-4)-α-D-Glcp	2.09
3ZT6[A,B,C,D]	α-D-Glcp-(1-4)-α-D-Glcp a-cyclodextrin	2.19
3ZT7[A,B,C,D]	α-D-Glcp-(1-4)-α-D-Glcp b-cyclodextrin	2.50
4CN1[A,B]	α-D-Glcp-(1-4)-α-D-Glcp	2.55
4CN4[A,B]	α-D-Glcp-(1-4)-β-D-Glcp2F-(1-4)-ASP	2.40
4CN6[A,B]	α-D-Glcp-(1-4)-β-D-Glcp	2.29
4U2Y[A,B]	α-D-Glcp-(1-1)-<non_carb>	2.48
4U2Z[A,B]	α-D-Glcp-(1-4)-α-D-Manp1F2F	2.26
4U31[A,B]	α-D-Glcp-(1-1)-<non_carb>	1.85
5CVS[A,B]	α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp	2.30
5LGV[A,B]	α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp	2.50
5LGW[A,B]	α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-6)[α-D-Glcp-(1-4)-α-D-Glcp-(1-4)]-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-β-D-Glcp	1.95
5VSJ[A,B]	α-D-Glcp-(1-1)-<non_carb>	2.46
5VT4[A,B,C,D]	α-D-Glcp-(1-1)-<non_carb>	3.21
7MEL[A,B]	α-D-Glcp-(1-1)-<non_carb>	1.75
7MGY[A,B]	α-D-Glcp-(1-1)-<non_carb>	1.83
7UVD[A,B]	α-D-Glcp-(1-1)-<non_carb>	2.73
8D6K[A,B]	α-D-Glcp-(1-1)-<non_carb>	2.73